| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,938,253 | A→G | 44.1% | H253H (CAT→CAC) | xerD ← | site‑specific tyrosine recombinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,938,253 | 0 | A | G | 44.1% | 19.5 / 75.0 | 93 | H253H (CAT→CAC) | xerD | site‑specific tyrosine recombinase |
| Reads supporting (aligned to +/- strand): ref base A (25/27); new base G (14/27); total (39/54) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.08e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 | |||||||||||
Only 100 of 111 total aligned reads displayed.
TGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGACGCAACACATGCGGTGACAGCTTTTCGCTGTCGATACCCGCCAGCACAG > minE/1938186‑1938320 | tggtggAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAAt < 1:108278/69‑1 (MQ=255)tggtggAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAAt < 1:3229210/69‑1 (MQ=255)tggtggAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAAt < 1:1852875/69‑1 (MQ=255) ggtggAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAATg < 1:357488/69‑1 (MQ=255) cGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAg > 1:428605/1‑69 (MQ=255) cGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAg > 1:283882/1‑69 (MQ=255) cGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAg > 1:3431638/1‑69 (MQ=255) ccAGTAGCATCTGCACCACGCGCAAATCCGCACCATGATTTAATAAGTGAGTGGCAAAAGCGTGACTCa < 1:502807/69‑1 (MQ=255) ccAGTAGCATCTGCACCACGCGCAAATCCGCACCATGATTTAATAAGTGAGTGGCAAAAGCGTGACGCa < 1:82827/69‑1 (MQ=255) ccAGTAGCATCTGCACCACGCGCAAATCCGCACCATGATTTAATAAGTGAGTGGCAAAAGCGTGACGCa < 1:1222736/69‑1 (MQ=255) ccAGTAGCATCTGCACCACGCGCAAATCCGCACCATGATTTAATAAGTGAGTGGCAAAAGCGTGACGCa < 1:1140483/69‑1 (MQ=255) ccAGTAGCATCTGCACCACGCGCAAATCCGCACCATGATTTAATAAGTGAGTGGCAAAAGCGTGACGCa < 1:3383325/69‑1 (MQ=255) ccAGTAGCATCTGCACCACGCGCAAATCCGCACCATGATTTAATAAGTGAGTGGCAAAAGCGTGACGCa < 1:1940934/69‑1 (MQ=255) ccAGTAGCATCTGCACCACGCGCAAATCCGCACCATGATTTAATAAGTGAGTGGCAAAAGCGTGACGCa < 1:3339364/69‑1 (MQ=255) ccAGTAGCATCTGCACCACGCGCAAATCCGCACCATGATTTAATAAGTGAGTGGCAAAAGCGTGACGCa < 1:3314084/69‑1 (MQ=255) ccAGTAGCATCTGCACCACGCGCAAATCCGCACCATGATTTAATAAGTGAGTGGCAAAAGCGTGACGCa < 1:2032157/69‑1 (MQ=255) ccAGTAGCATCTGCACCACGCGCAAATCCGCACCATGATTTAATAAGTGAGTGGCAAAAGCGTGACGCa < 1:1792729/69‑1 (MQ=255) ccAGTAGCATCTGCACCACGCGCAAATCCGCACCATGATTTAATAAGTGAGTGGCAAAAGCGTGACGCa < 1:2997469/69‑1 (MQ=255) ccAGTAGCATCTGCACCACGCGCAAATCCGCACCATGATTTAATAAGTGAGTGGCAAAAGCGTGACGCa < 1:2184208/69‑1 (MQ=255) ccAGTAGCATCTGCACCACGCGCAAATCCGCACCATGATTTAATAAGTGAGTGGCAAAAGCGTGACGCa < 1:2992552/69‑1 (MQ=255) ccAGTAGCATCTGCACCACGCGCAAATCCGCACCATGATTTAATAAGTGAGTGGCAAAAGCGTGACGCa < 1:295012/69‑1 (MQ=255) ccAGTAGCATCTGCACCACGCGCAAATCCGCACCATGATTTAATAAGTGAGTGGCAAAAGCGTGACGCa < 1:2462475/69‑1 (MQ=255) ccAGTAGCATCTGCACCACGCGCAAATCCGCACCATGATTTAATAAGTGAGTGGCAAAAGCGTGACGCa < 1:2863038/69‑1 (MQ=255) ccAGTAGCATCTGCACCACGCGCAAATCCGCACCATGATTTAATAAGTGAGTGGCAAAAGCGTGACGCa < 1:862896/69‑1 (MQ=255) ccAGTAGCATCTGCACCACGCGCAAATCCGCACCATGATTTAATAAGTGAGTGGCAAAAGCGTGACGCa < 1:797978/69‑1 (MQ=255) ccAGTAGCATCTGCACCACGCGCAAATCCGCACCATGATTTAATAAGTGAGTGGCAAAAGCGTGACGCa < 1:1704433/69‑1 (MQ=255) ccAGTAGCATCTGCACCACGCGCAAATCCGCACCATGATTTAATAAGTGAGTGGCAAAAGCGTGACGCa < 1:1347524/69‑1 (MQ=255) ccAGTAGCATCTGCACCACGCGCAAATCCGCACCATGATTTAATAAGTGAGTGGCAAAAGCGTGACGCa < 1:1653190/69‑1 (MQ=255) ccAGTAGCATCTGCACCACGCGCAAATCCGCACCATGATTTAATAAGTGAGTGGCAAAAGCGTGACGCa < 1:1353057/69‑1 (MQ=255) ccAGTAGCATCTGCACCACGCGCAAATCCGCACCATGATTTAATAAGTGAGTGGCAAAAGCGTGACGCa < 1:599293/69‑1 (MQ=255) ccAGTAGCATCTGCACCACGCGCAAATCCGCACCATGATTTAATAAGTGAGTGGCAAAAGCGTGACGCa < 1:1498877/69‑1 (MQ=255) ccAGTAGCATCTGCACCACGCGCAAATCCGCACCATGATTTAATAAGTGAGTGGCAAAAGCGTGACGCa < 1:1422129/69‑1 (MQ=255) gTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGACGCAaca < 1:1971454/69‑1 (MQ=255) gTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGACGCAaca < 1:1990500/69‑1 (MQ=255) gCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGACGCAACACAt > 1:1703390/1‑69 (MQ=255) gCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGACGCAACACAt > 1:1646881/1‑69 (MQ=255) tGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGa > 1:523821/1‑54 (MQ=255) tGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGa > 1:1239542/1‑54 (MQ=255) tGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGa > 1:2383447/1‑54 (MQ=255) tGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGa > 1:3223931/1‑54 (MQ=255) tGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGa > 1:465949/1‑54 (MQ=255) tGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGa > 1:435877/1‑54 (MQ=255) tGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGa > 1:1753262/1‑54 (MQ=255) tGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGa > 1:807148/1‑54 (MQ=255) tGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGACGCAaca < 1:3138009/61‑1 (MQ=255) tGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGACGCAaca > 1:1059824/1‑61 (MQ=255) tGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGACGCAaca < 1:666335/61‑1 (MQ=255) tGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGACGCAaca < 1:808725/61‑1 (MQ=255) tGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGACGCAaca > 1:102379/1‑61 (MQ=255) tGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGACGCAaca > 1:1691544/1‑61 (MQ=255) tGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGACGCAaca < 1:1991035/61‑1 (MQ=255) tGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGACGCAaca > 1:1360913/1‑61 (MQ=255) tGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGACGCAaca < 1:2063796/61‑1 (MQ=255) tGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGACGCAaca < 1:2112318/61‑1 (MQ=255) tGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGACGCAaca > 1:2246609/1‑61 (MQ=255) tGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGACGCAaca < 1:1176957/61‑1 (MQ=255) tGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTAGCAAAAGCGTGa > 1:1157842/1‑54 (MQ=255) tGCACCACGCGTAAATCCGCACCATGATTTAATAAAAGAGTGGCAAAAg > 1:1127597/1‑49 (MQ=255) tGCACCACGCGCAAATCCGCACCATGATTTAATAAGTGAg > 1:328414/1‑40 (MQ=38) tGCACCACGCGCAAATCCGCACCATGATTTAATAAGTGAGTGGCAAAAGCGTGACGCAACACATGCGGt > 1:476440/1‑69 (MQ=255) tGCACCACGCGCAAATCCGCACCATGATTTAATAAGTGAGTGGCAAAAGCGTGACGCAACACATGCGGt > 1:1861574/1‑69 (MQ=255) tGCACCACGCGCAAATCCGCACCATGATTTAATAAGTGAGTGGCAAAAGCGTGACGCAACACATGCGGt > 1:588904/1‑69 (MQ=255) tGCACCACGCGCAAATCCGCACCATGATTTAATAAGTGAGTGGCAAAAGCGTGACGCAACACATGCGGt > 1:2787190/1‑69 (MQ=255) tGCACCACGCGCAAATCCGCACCATGATTTAATAAGTGAGTGGCAAAAGCGTGACGCAACACATGCGGt > 1:3335642/1‑69 (MQ=255) tGCACCACGCGCAAATCCGCACCATGATTTAATAAGTGAGTGGCAAAAGCGTGACGCAACACATGCGGt > 1:669749/1‑69 (MQ=255) tGCACCACGCGCAAATCCGCACCATGATTTAATAAGTGAGTGGCAAAAGCGTGACGCAACACATGCGGt > 1:3267203/1‑69 (MQ=255) tGCACCACGCGCAAATCCGCACCATGATTTAATAAGTGAGTGGCAAAAGCGTGACGCAACACATGCGGt > 1:449692/1‑69 (MQ=255) tGCACCACGCGCAAATCCGCACCATGATTTAATAAGTGAGTGGCAAAAGCGTGACGCAACACATGCGGt > 1:3171997/1‑69 (MQ=255) tGCACCACGCGCAAATCCGCACCATGATTTAATAAGTGAGTGGCAAAAGCGTGACGCAACACATGCGGt > 1:690640/1‑69 (MQ=255) tGCACCACGCGCAAATCCGCACCATGATTTAATAAGTGAGTGGCAAAAGCGTGACGCAACACATGCGGt > 1:3026316/1‑69 (MQ=255) tGCACCACGCGCAAATCCGCACCATGATTTAATAAGTGAGTGGCAAAAGCGTGACGCAACACATGCGGt > 1:2116239/1‑69 (MQ=255) tGCACCACGCGCAAATCCGCACCATGATTTAATAAGTGAGTGGCAAAAGCGTGACGCAACACATGCGGt > 1:292392/1‑69 (MQ=255) tGCACAACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGACGCAaca < 1:2956824/61‑1 (MQ=255) ccacGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGACGCAACACATGCGGt < 1:1711552/65‑1 (MQ=255) ccacGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGACGCAACACATGCGGt < 1:747935/65‑1 (MQ=255) ccacGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGACGCAACACATGCGGt < 1:835580/65‑1 (MQ=255) ccacGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGACGCAACACATGCGGt < 1:3193060/65‑1 (MQ=255) gTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTCACGCAaca < 1:2644274/51‑1 (MQ=255) cGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGACGCAACACATGCGGTGACAGCTTTTCGCTGTc < 1:2821440/69‑1 (MQ=255) cGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGACGCAACACATGCGGTGACAGCTTTTCGCTGTc < 1:1517854/69‑1 (MQ=255) cGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGACGCAACACATGCGGTGACAGCTTTTCGCTGTc < 1:2013395/69‑1 (MQ=255) cACCATGATTTAATAAATGAGTGGCAAAAGCGTGACGCAACACATGCGGTGACAGCTTTTCTCTGTCGa < 1:1369256/69‑1 (MQ=255) cACCATGATTTAATAAATGAGTGGCAAAAGCGTGACGCAACACATGCGGTGACAGCTTTTCGCTGTCGa < 1:225426/69‑1 (MQ=255) cATGATTTAATAAATGAGTGGCAAAAGCGTGACGCAACACATGCGGTGACAGCTTTTCGCTGTc < 1:2773158/64‑1 (MQ=255) aaTGAGTGGCAAAAGCGTGACGCAACACATGCGGTGACAGCTTTTCGCTGTCGATAccc < 1:1522449/59‑1 (MQ=255) aaTGAGTGGCAAAAGCGTGACGCAACACATGCGGTGACAGCTTTTCGCTGTCGATAccc < 1:2575756/59‑1 (MQ=255) aaTGAGTGGCAAAAGCGTGACGCAACACATGCGGTGACAGCTTTTCGCTGTCGATAccc < 1:79884/59‑1 (MQ=255) aaTGAGTGGCAAAAGCGTGACGCAACACATGCGGTGACAGCTTTTCGCTGTCGATACCCGCcagcacag > 1:2639401/1‑69 (MQ=255) aaTGAGTGGCAAAAGCGTGACGCAACACATGCGGTGACAGCTTTTCGCTGTCGATACCCGCcagcacag > 1:429383/1‑69 (MQ=255) aaTGAGTGGCAAAAGCGTGACGCAACACATGCGGTGACAGCTTTTCGCTGTCGATACCCGCcagcacag > 1:818311/1‑69 (MQ=255) aaTGAGTGGCAAAAGCGTGACGCAACACATGCGGTGACAGCTTTTCGCTGTCGATACCCGCcagcacag > 1:1227918/1‑69 (MQ=255) aaTGAGTGGCAAAAGCGTGACGCAACACATGCGGTGACAGCTTTTCGCTGTCGATACCCGCcagcacag > 1:796313/1‑69 (MQ=255) aaTGAGTGGCAAAAGCGTGACGCAACACATGCGGTGACAGCTTTTCGCTGTCGATACCCGCcagcacag > 1:750943/1‑69 (MQ=255) aaTGAGTGGCAAAAGCGTGACGCAACACATGCGGTGACAGCTTTTCGCTGTCGATACCCGCcagcacag > 1:2112966/1‑69 (MQ=255) aaTGAGTGGCAAAAGCGTGACGCAACACATGCGGTGACAGCTTTTCGCTGTCGATACCCGCcagcacag > 1:3336806/1‑69 (MQ=255) aaTGAGTGGCAAAAGCGTGACGCAACACATGCGGTGACAGCTTTTCGCTGTCGATACCCGCcagcacag > 1:1900376/1‑69 (MQ=255) aaTGAGTGGCAAAAGCGTGACGCAACACATGCGGTGACAGCTTTTCGCTGTCGATACCCGCcagcacag > 1:1746580/1‑69 (MQ=255) aaTGAGTGGCAAAAGCGTGACGCAACACATGCGGTGACAGCTTTTCGCTGTCGATACCCGCcagcacag > 1:469028/1‑69 (MQ=255) aaTGAGTGGCAAAAGCGTGACGCAACACATGCGGTGACAGCTTTTCGCTGTCGATACCCGCcagcaca > 1:1539002/1‑68 (MQ=255) aTGAGTGGCAAAAGCGTGACGCAACACATGCGGTGACAGCTTTTCGCTGTCGATAccc < 1:2629112/58‑1 (MQ=255) | TGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGACGCAACACATGCGGTGACAGCTTTTCGCTGTCGATACCCGCCAGCACAG > minE/1938186‑1938320 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |