| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,940,709 | T→C | 27.8% | G43G (GGT→GGC) | ygfZ → | predicted folate‑dependent regulatory protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,940,709 | 0 | T | C | 27.8% | 85.5 / 35.9 | 86 | G43G (GGT→GGC) | ygfZ | predicted folate‑dependent regulatory protein |
| Reads supporting (aligned to +/- strand): ref base T (36/26); new base C (18/6); total (54/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.14e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.41e-02 | |||||||||||
GCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCA > minE/1940649‑1940765 | gCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGAc > 1:1234808/1‑69 (MQ=255)gCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGAc > 1:1521967/1‑69 (MQ=255)gCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGAc > 1:144865/1‑69 (MQ=255)gCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGAc > 1:1527790/1‑69 (MQ=255)gCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGAc > 1:432845/1‑69 (MQ=255)gCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGAc > 1:1317635/1‑69 (MQ=255)gCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGAc > 1:3351994/1‑69 (MQ=255)gCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGAc > 1:3074080/1‑69 (MQ=255)gCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGAc > 1:762774/1‑69 (MQ=255)gCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGAAGGGTCa > 1:2578065/1‑63 (MQ=255)gCTTGATGACTGGGCGCTTGCCACCATTAATGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGAc > 1:2516048/1‑69 (MQ=255) tgaCTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGa > 1:2257208/1‑69 (MQ=255) gggCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCa > 1:2513820/1‑69 (MQ=255) gcTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCa < 1:1878518/69‑1 (MQ=255) gcTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCa < 1:3186942/69‑1 (MQ=255) gcTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCa < 1:2543151/69‑1 (MQ=255) gcTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCa < 1:1741650/69‑1 (MQ=255) gcTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCa < 1:2690024/69‑1 (MQ=255) gcTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCa < 1:2817538/69‑1 (MQ=255) tgacaccaTTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGAt > 1:3338139/4‑69 (MQ=255) tcccaccaTTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGAt > 1:3228132/3‑69 (MQ=255) tGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGAt > 1:722400/1‑69 (MQ=255) tGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGAt > 1:60604/1‑69 (MQ=255) tGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGAt > 1:137717/1‑69 (MQ=255) tGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGAt > 1:1339892/1‑69 (MQ=255) tGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGAt > 1:2090013/1‑69 (MQ=255) tGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGAt > 1:131452/1‑69 (MQ=255) ccaTTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCa < 1:2152518/61‑1 (MQ=255) aTTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGcc > 1:1082707/1‑58 (MQ=255) aTTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGcc > 1:2301574/1‑58 (MQ=255) aTTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCa > 1:1945061/1‑59 (MQ=255) aTTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCa > 1:1478287/1‑59 (MQ=255) aTTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCa > 1:885405/1‑59 (MQ=255) aTTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCa > 1:3003436/1‑59 (MQ=255) aTTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCa > 1:1179592/1‑59 (MQ=255) aTTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCa > 1:2498157/1‑59 (MQ=255) aTTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCa > 1:2399095/1‑59 (MQ=255) aTTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCa > 1:1728813/1‑59 (MQ=255) aTTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCa > 1:1902454/1‑59 (MQ=255) aTTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCa > 1:346992/1‑59 (MQ=255) aTTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCa > 1:1898600/1‑59 (MQ=255) aTTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCa > 1:1877705/1‑59 (MQ=255) aTTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCa > 1:3344080/1‑59 (MQ=255) ttACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCagaag < 1:1162579/69‑1 (MQ=255) ggCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAg < 1:1396840/69‑1 (MQ=255) ggCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAg < 1:2672796/69‑1 (MQ=255) ggCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAg < 1:116570/69‑1 (MQ=255) ggCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAg < 1:497588/69‑1 (MQ=255) ggCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAg < 1:177899/69‑1 (MQ=255) ggCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAg < 1:1258583/69‑1 (MQ=255) ggCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAg < 1:557450/69‑1 (MQ=255) ggCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAg < 1:3275531/69‑1 (MQ=255) ggCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAg < 1:2191651/69‑1 (MQ=255) gcgcGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAg < 1:1554521/68‑1 (MQ=255) gcgGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCa < 1:2319841/68‑1 (MQ=255) gcgGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCa < 1:3143996/68‑1 (MQ=255) gcgGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCa < 1:2853765/68‑1 (MQ=255) gcgGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCa < 1:2703698/68‑1 (MQ=255) gcgGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCa < 1:1964884/68‑1 (MQ=255) gcgGACAGCGAAAAATATATGCAGGGTCAGGTGACACCAGATGTCAGCCAGATGGCAGAAGATCAGCa < 1:2197716/68‑1 (MQ=255) cgGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCa < 1:2148002/67‑1 (MQ=255) cAGCGAAAAATATATGCAAGGCCAGGTGACAGCAGATGTCAGCCAGATGACTGAAGATCAGCACCTGCt < 1:1101832/69‑1 (MQ=255) cAGCGAAAAATATATGCAAGGCCAGGTGACAGCAGATGTCAGCCAGATGACTGAAGATCAGCACCTGCt < 1:872869/69‑1 (MQ=255) cAGCGAAAAATATATGCAAGGCCAGGTGACAGCAGATGTCAGCCAGATGACTGAAGATCAGCACCTGCt < 1:2523523/69‑1 (MQ=255) cAGCGAAAAATATATGCAAGGCCAGGTGACAGCAGATGTCAGCCAGATGACTGAAGATCAGCACCTGCt < 1:798537/69‑1 (MQ=255) cAGCGAAAAATATATGCAAGGCCAGGTGACAGCAGATGTCAGCCAGATGACTGAAGATCAGCACCTGCt < 1:1146191/69‑1 (MQ=255) cAGCGAAAAATATATGCAAGGCCAGGTGACAGCAGATGTCAGCCAGATGACTGAAGATCAGCACCTGCt < 1:1299593/69‑1 (MQ=255) aaaaaTATATGCAGGGTCAGGTGACAGCAGATGTCAGCCa < 1:396915/40‑1 (MQ=255) atatatGCAAGGCCAGGTGACAGCAGATGTCAGCCAGATGACTGAAGATCAGCACCTGCTcgccgc > 1:3123724/1‑66 (MQ=255) atatatGCAAGGCCAGGTGACAGCAGATGTCAGCCAGATGACTGAAGATCAGCACCTGCTCGCCGccc > 1:401785/1‑68 (MQ=255) atatatGCAAGGCCAGGTGACAGCAGATGTCAGCCAGATGACTGAAGATCAGCACCTGCTCGCCGCCCa > 1:12029/1‑69 (MQ=255) atatatGCAAGGCCAGGTGACAGCAGATGTCAGCCAGATGACTGAAGATCAGCACCTGCTCGCCGCCCa > 1:969133/1‑69 (MQ=255) atatatGCAAGGCCAGGTGACAGCAGATGTCAGCCAGATGACTGAAGATCAGCACCTGCTCGCCGCCCa > 1:2997910/1‑69 (MQ=255) atatatGCAAGGCCAGGTGACAGCAGATGTCAGCCAGATGACTGAAGATCAGCACCTGCTCGCCGCCCa > 1:2590218/1‑69 (MQ=255) atatatGCAAGGCCAGGTGACAGCAGATGTCAGCCAGATGACTGAAGATCAGCACCTGCTCGCCGCCCa > 1:7788/1‑69 (MQ=255) atatatGCAAGGCCAGGTGACAGCAGATGTCAGCCAGATGACTGAAGATCAGCACCTGCTCGCCGCCCa > 1:77759/1‑69 (MQ=255) atatatGCAAGGCCAGGTGACAGCAGATGTCAGCCAGATGACTGAAGATCAGCACCTGCTCGCCGCCCa > 1:2442312/1‑69 (MQ=255) atatatGCAAGGCCAGGTGACAGCAGATGTCAGCCAGATGACTGAAGATCAGCACCTGCTCGCCGCCCa > 1:1179301/1‑69 (MQ=255) atatatGCAAGGCCAGGTGACAGCAGATGTCAGCCAGATGACTGAAGATCAGCACCTGCTCGCCGCCCa > 1:2044883/1‑69 (MQ=255) atatatGCAAGGCCAGGTGACAGCAGATGTCAGCCAGATGACTGAAGATCAGCACCTGCTCGCCGCCCa > 1:1032034/1‑69 (MQ=255) atatatGCAAGGCCAGGTGACAGCAGATGTCAGCCAGATGACTGAAGATCAGCACCTGCTCGCCGCCCa > 1:1468270/1‑69 (MQ=255) atatatGCAAGGCCAGGTGACAGCAGATGTCAGCCAGATGACTGAAGATCAGCACCTGCTCGCCGCCCa > 1:45782/1‑69 (MQ=255) atatatGCAAGGCCAGGTGACAGCAGATGTCAGCCAGATGACTGAAGATCAGCACCTGCTCGCCGCCCa > 1:1682378/1‑69 (MQ=255) atatatGCAAGGCCAGGTGACAGCAGATGTCAGCCAGATGACTGAAGATCAGCACCTGCTCGCCGCCCa > 1:2788958/1‑69 (MQ=255) atatatGCAAGGCCAGGTGACAGCAGATGTCAGCCAGATGACTGAAGATCAGCACCTGCTCGCCGCCCa > 1:2129011/1‑69 (MQ=255) atatatGCAAGGCCAGGTGACAGCAGATGTCAGCCAGATGACTGAAGATCAGCACCTGCTCGCCGCCCa > 1:1973466/1‑69 (MQ=255) | GCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCA > minE/1940649‑1940765 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |