| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,940,739 | A→T | 34.3% | A53A (GCA→GCT) ‡ | ygfZ → | predicted folate‑dependent regulatory protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,940,739 | 0 | A | T | 34.3% | 66.5 / 52.7 | 70 | A53A (GCA→GCT) ‡ | ygfZ | predicted folate‑dependent regulatory protein |
| Reads supporting (aligned to +/- strand): ref base A (24/22); new base T (18/6); total (42/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.68e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTA > minE/1940675‑1940799 | ttACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCagaag < 1:1162579/69‑1 (MQ=255) ggCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAg < 1:2672796/69‑1 (MQ=255) ggCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAg < 1:116570/69‑1 (MQ=255) ggCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAg < 1:177899/69‑1 (MQ=255) ggCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAg < 1:1258583/69‑1 (MQ=255) ggCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAg < 1:3275531/69‑1 (MQ=255) ggCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAg < 1:2191651/69‑1 (MQ=255) ggCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAg < 1:1396840/69‑1 (MQ=255) ggCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAg < 1:557450/69‑1 (MQ=255) ggCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAg < 1:497588/69‑1 (MQ=255) gcgcGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAg < 1:1554521/68‑1 (MQ=255) gcgGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCa < 1:2703698/68‑1 (MQ=255) gcgGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCa < 1:2853765/68‑1 (MQ=255) gcgGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCa < 1:1964884/68‑1 (MQ=255) gcgGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCa < 1:3143996/68‑1 (MQ=255) gcgGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCa < 1:2319841/68‑1 (MQ=255) gcgGACAGCGAAAAATATATGCAGGGTCAGGTGACACCAGATGTCAGCCAGATGGCAGAAGATCAGCa < 1:2197716/68‑1 (MQ=255) cgGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCa < 1:2148002/67‑1 (MQ=255) cAGCGAAAAATATATGCAAGGCCAGGTGACAGCAGATGTCAGCCAGATGACTGAAGATCAGCACCTGCt < 1:2523523/69‑1 (MQ=255) cAGCGAAAAATATATGCAAGGCCAGGTGACAGCAGATGTCAGCCAGATGACTGAAGATCAGCACCTGCt < 1:1299593/69‑1 (MQ=255) cAGCGAAAAATATATGCAAGGCCAGGTGACAGCAGATGTCAGCCAGATGACTGAAGATCAGCACCTGCt < 1:798537/69‑1 (MQ=255) cAGCGAAAAATATATGCAAGGCCAGGTGACAGCAGATGTCAGCCAGATGACTGAAGATCAGCACCTGCt < 1:872869/69‑1 (MQ=255) cAGCGAAAAATATATGCAAGGCCAGGTGACAGCAGATGTCAGCCAGATGACTGAAGATCAGCACCTGCt < 1:1146191/69‑1 (MQ=255) cAGCGAAAAATATATGCAAGGCCAGGTGACAGCAGATGTCAGCCAGATGACTGAAGATCAGCACCTGCt < 1:1101832/69‑1 (MQ=255) atatatGCAAGGCCAGGTGACAGCAGATGTCAGCCAGATGACTGAAGATCAGCACCTGCTcgccgc > 1:3123724/1‑66 (MQ=255) atatatGCAAGGCCAGGTGACAGCAGATGTCAGCCAGATGACTGAAGATCAGCACCTGCTCGCCGccc > 1:401785/1‑68 (MQ=255) atatatGCAAGGCCAGGTGACAGCAGATGTCAGCCAGATGACTGAAGATCAGCACCTGCTCGCCGCCCa > 1:2788958/1‑69 (MQ=255) atatatGCAAGGCCAGGTGACAGCAGATGTCAGCCAGATGACTGAAGATCAGCACCTGCTCGCCGCCCa > 1:2997910/1‑69 (MQ=255) atatatGCAAGGCCAGGTGACAGCAGATGTCAGCCAGATGACTGAAGATCAGCACCTGCTCGCCGCCCa > 1:2442312/1‑69 (MQ=255) atatatGCAAGGCCAGGTGACAGCAGATGTCAGCCAGATGACTGAAGATCAGCACCTGCTCGCCGCCCa > 1:45782/1‑69 (MQ=255) atatatGCAAGGCCAGGTGACAGCAGATGTCAGCCAGATGACTGAAGATCAGCACCTGCTCGCCGCCCa > 1:77759/1‑69 (MQ=255) atatatGCAAGGCCAGGTGACAGCAGATGTCAGCCAGATGACTGAAGATCAGCACCTGCTCGCCGCCCa > 1:2590218/1‑69 (MQ=255) atatatGCAAGGCCAGGTGACAGCAGATGTCAGCCAGATGACTGAAGATCAGCACCTGCTCGCCGCCCa > 1:7788/1‑69 (MQ=255) atatatGCAAGGCCAGGTGACAGCAGATGTCAGCCAGATGACTGAAGATCAGCACCTGCTCGCCGCCCa > 1:969133/1‑69 (MQ=255) atatatGCAAGGCCAGGTGACAGCAGATGTCAGCCAGATGACTGAAGATCAGCACCTGCTCGCCGCCCa > 1:1032034/1‑69 (MQ=255) atatatGCAAGGCCAGGTGACAGCAGATGTCAGCCAGATGACTGAAGATCAGCACCTGCTCGCCGCCCa > 1:12029/1‑69 (MQ=255) atatatGCAAGGCCAGGTGACAGCAGATGTCAGCCAGATGACTGAAGATCAGCACCTGCTCGCCGCCCa > 1:1682378/1‑69 (MQ=255) atatatGCAAGGCCAGGTGACAGCAGATGTCAGCCAGATGACTGAAGATCAGCACCTGCTCGCCGCCCa > 1:1973466/1‑69 (MQ=255) atatatGCAAGGCCAGGTGACAGCAGATGTCAGCCAGATGACTGAAGATCAGCACCTGCTCGCCGCCCa > 1:1468270/1‑69 (MQ=255) atatatGCAAGGCCAGGTGACAGCAGATGTCAGCCAGATGACTGAAGATCAGCACCTGCTCGCCGCCCa > 1:1179301/1‑69 (MQ=255) atatatGCAAGGCCAGGTGACAGCAGATGTCAGCCAGATGACTGAAGATCAGCACCTGCTCGCCGCCCa > 1:2044883/1‑69 (MQ=255) atatatGCAAGGCCAGGTGACAGCAGATGTCAGCCAGATGACTGAAGATCAGCACCTGCTCGCCGCCCa > 1:2129011/1‑69 (MQ=255) tGACAGCAGATGTCAGCCATATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGt > 1:2459261/1‑68 (MQ=255) tGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCAtt > 1:1335464/1‑54 (MQ=255) tGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGt > 1:852968/1‑68 (MQ=255) tGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGt > 1:54084/1‑68 (MQ=255) gACAGCAGATGTCAGCCAGATGGCATAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTaa > 1:11476/1‑69 (MQ=255) gACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGt > 1:224024/1‑67 (MQ=255) gACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTaa > 1:995108/1‑69 (MQ=255) gACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTaa > 1:1382514/1‑69 (MQ=255) gACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTaa > 1:2330921/1‑69 (MQ=255) gACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTaa > 1:531699/1‑69 (MQ=255) gACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTaa > 1:1495388/1‑69 (MQ=255) gACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTaa > 1:496911/1‑69 (MQ=255) gACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTaa > 1:1737244/1‑69 (MQ=255) gACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTaa > 1:2042025/1‑69 (MQ=255) gACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTaa < 1:2820166/69‑1 (MQ=255) gACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTaa > 1:2835368/1‑69 (MQ=255) gACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTaa > 1:2862709/1‑69 (MQ=255) gACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTaa < 1:1927984/69‑1 (MQ=255) gACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTaa > 1:3214738/1‑69 (MQ=255) gcagATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAAtg > 1:326742/1‑69 (MQ=255) gcagATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAAtg > 1:457849/1‑69 (MQ=255) gcagATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAAtg > 1:2796455/1‑69 (MQ=255) gcagATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAAtg > 1:2303892/1‑69 (MQ=255) gcagATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCAATTGCGACGCCAAAGGTaaaa > 1:1646767/1‑67 (MQ=255) gATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTgg < 1:376730/69‑1 (MQ=255) gTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGccc < 1:500998/40‑1 (MQ=255) gTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGccc < 1:2863339/40‑1 (MQ=255) cAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTa > 1:190030/1‑69 (MQ=255) cAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTa > 1:2002756/1‑69 (MQ=255) | TTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTA > minE/1940675‑1940799 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |