| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,941,264 | T→C | 54.7% | C228C (TGT→TGC) | ygfZ → | predicted folate‑dependent regulatory protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,941,264 | 0 | T | C | 54.7% | 7.3 / 40.9 | 53 | C228C (TGT→TGC) | ygfZ | predicted folate‑dependent regulatory protein |
| Reads supporting (aligned to +/- strand): ref base T (17/7); new base C (18/11); total (35/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.69e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.83e-01 | |||||||||||
AGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGTTATACCGGACAAGAGATGGTGGCGCGAGCAAAATTCCGTGGTGCCAATAAACG > minE/1941196‑1941317 | aGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCTGTATCAGCTTTAAGAAAGGCTGt < 1:1731497/69‑1 (MQ=255)aGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGt < 1:3241144/69‑1 (MQ=255)aGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGt < 1:3186099/69‑1 (MQ=255)aGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGt < 1:2573987/69‑1 (MQ=255)aGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGt < 1:2528666/69‑1 (MQ=255)aGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGt < 1:1705205/69‑1 (MQ=255)aGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGt < 1:2455766/69‑1 (MQ=255)aGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGt < 1:2415906/69‑1 (MQ=255)aGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGt < 1:211389/69‑1 (MQ=255) gCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGt < 1:3067506/68‑1 (MQ=255) ttATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGTTATACCGGAc < 1:890764/69‑1 (MQ=255) ttATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGTTATACCGGAc < 1:3395666/69‑1 (MQ=255) tATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGTTATACCgg > 1:1202850/1‑66 (MQ=255) tATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGTTATACCgg > 1:733169/1‑66 (MQ=255) tATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGTTATACCgg > 1:480060/1‑66 (MQ=255) tATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGTTATACCgg > 1:2106564/1‑66 (MQ=255) tATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGTTATACCgg > 1:1521968/1‑66 (MQ=255) ccACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGTTATACCGGTCAaga < 1:3110625/68‑1 (MQ=255) ccACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGTTATACCGGACAaga < 1:2945847/68‑1 (MQ=255) ccACAGGCAACCAATCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGTTATACCGGACAaga < 1:3158787/68‑1 (MQ=255) acaGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGTTATACCGGACAAGAGAt < 1:555666/69‑1 (MQ=255) acaGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGTTATACCGGACAAGAGAt < 1:3173143/69‑1 (MQ=255) gACCAACCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGCTATACCGGACAaga > 1:1612778/1‑60 (MQ=255) gACCAACCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGCTATACCGGACAaga > 1:2044616/1‑60 (MQ=255) gACCAACCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGCTATACCGGACAaga > 1:298939/1‑60 (MQ=255) gACCAACCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGCTATACCGGACAaga > 1:2914403/1‑60 (MQ=255) gACCAACCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGCTATACCGGACAaga > 1:1290642/1‑60 (MQ=255) gACCAACCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGCTATACCGGACAaga > 1:2599304/1‑60 (MQ=255) gACCAACCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGCTATACCGGACAaga > 1:1637265/1‑60 (MQ=255) gACCAACCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGCTATACCGGACAaga > 1:3176034/1‑60 (MQ=255) gACCAACCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGCTATACCGGACAaga > 1:1848662/1‑60 (MQ=255) gACCAACCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGCTATACCGGACAaga > 1:669650/1‑60 (MQ=255) gACCAACCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGCTATACCGGACAaga > 1:323304/1‑60 (MQ=255) gACCAACCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGCTATACCGGACAaga > 1:704687/1‑60 (MQ=255) gACCAACCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGCTATACCGGACAaga > 1:417256/1‑60 (MQ=255) gACCAACCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGCTATACCGGACAaga > 1:3262733/1‑60 (MQ=255) gACCAACCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGCTATACCGGACAag > 1:1425842/1‑59 (MQ=255) cAATCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGt < 1:1778892/43‑1 (MQ=255) cAATCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGt < 1:453038/43‑1 (MQ=255) aGCTTTAAGAAAGGCTGTTATACCGGACAAGAGATGGTGGCGCGAGCAGAATTCCGTGGTGCCAATaaa > 1:1139099/1‑69 (MQ=255) aGCTTTAAGAAAGGCTGTTATACCGGACAAGAGATGGTGGCGCGAGCAAAATTCCGTGGTGCCAATaaa > 1:158441/1‑69 (MQ=255) aGCTTTAAGAAAGGCTGTTATACCGGACAAGAGATGGTGGCGCGAGCAAAATTCCGTGGTGCCAATaaa > 1:3037744/1‑69 (MQ=255) aGCTTTAAGAAAGGCTGTTATACCGGACAAGAGATGGTGGCGCGAGCAAAATTCCGTGGTGCCAATaaa > 1:504319/1‑69 (MQ=255) aGCTTTAAGAAAGGCTGTTATACCGGACAAGAGATGGTGGCGCGAGCAAAATTCCGTGGTGCCAATaaa > 1:2577743/1‑69 (MQ=255) aGCTTTAAGAAAGGCTGTTATACCGGACAAGAGATGGTGGCGCGAGCAAAATTCCGTGGTGCCAATaaa > 1:2512112/1‑69 (MQ=255) aGCTTTAAGAAAGGCTGTTATACCGGACAAGAGATGGTGGCGCGAGCAAAATTCCGTGGTGCCAATaaa > 1:2473516/1‑69 (MQ=255) aGCTTTAAGAAAGGCTGTTATACCGGACAAGAGATGGTGGCGCGAGCAAAATTCCGTGGTGCCAATaaa > 1:703797/1‑69 (MQ=255) aGCTTTAAGAAAGGCTGTTATACCGGACAAGAGATGGTGGCGCGAGCAAAATTCCGTGGTGCCAATaaa > 1:707696/1‑69 (MQ=255) aGCTTTAAGAAAGGCTGTTATACCGGACAAGAGATGGTGGCGCGAGCAAAATTCCGTGGTGCCAATaaa > 1:369656/1‑69 (MQ=255) aGCTTTAAGAAAGGCTGTTATACCGGACAAGAGATGGTGGCGCGAGCAAAATTCCGTGGTGCCAATaaa > 1:3350238/1‑69 (MQ=255) aGCTTTAAGAAAGGCTGTTATACCGGACAAGAGATGGTGGCGCGAGCAAAATTCCGTGGTGCCAATaaa > 1:3417425/1‑69 (MQ=255) aGCTTTAAGAAAGGCTGCTATACCGGACAAGAGATGGTGGCGCGAGCAAAATTCCGTGGTGCcaacaa < 1:1253968/68‑1 (MQ=255) aGCTTTAAGAAAGGCTGCTATACCGGACAAGAGATGGTGGCGCGAGCAAAATTCCGTGGTGCcaacaa < 1:3093395/68‑1 (MQ=255) aGCTTTAAGAAAGGCTGCTATACCGGACAAGAGATGGTGGCGCGAGCAAAATTCCGTGGTGCcaacaa < 1:1430929/68‑1 (MQ=255) aGCTTTAAGAAAGGCTGCTATACCGGACAAGAGATGGTGGCGCGAGCAAAATTCCGTGGTGCcaacaa < 1:2851339/68‑1 (MQ=255) aGCTTTAAGAAAGGCTGCTATACCGGACAAGAGATGGTGGCGCGAGCAAAATTCCGTGGTGCcaacaa < 1:2807958/68‑1 (MQ=255) aGCTTTAAGAAAGGCTGCTATACCGGACAAGAGATGGTGGCGCGAGCAAAATTCCGTGGTGCcaacaa < 1:2796636/68‑1 (MQ=255) aGCTTTAAGAAAGGCTGCTATACCGGACAAGAGATGGTGGCGCGAGCAAAATTCCGTGGTGCcaacaa < 1:2324299/68‑1 (MQ=255) aGCTTTAAGAAAGGCTGCTATACCGGACAAGAGATGGTGGCGCGAGCAAAATTCCGTGGTGCcaacaa < 1:2242180/68‑1 (MQ=255) aGCTTTAAGAAAGGCTGCTATACCGGACAAGAGATGGTGGCGCGAGCAAAATTCCGTGGTGCcaacaa < 1:2240679/68‑1 (MQ=255) aGCTTTAAGAAAGGCTGCTATACCGGACAAGAGATGGTGGAGCGAGCAAAATTCCGTGGTGCcaacaa < 1:1945229/68‑1 (MQ=255) gCTTTAAGAAAGGCTGCTATACCGGACAAGAGATGGTGGCGCGAGCAAAATTCCGTGGTGCcaacaa < 1:2222959/67‑1 (MQ=255) cTTTAAGAAAGGCTGCTATACCGGACAAGAGATGGTGGCGCGAGCAAAATTCCGTGGTGCCAACAAAc > 1:3312277/1‑68 (MQ=255) cTTTAAGAAAGGCTGCTATACCGGACAAGAGATGGTGGCGCGAGCAAAATTCCGTGGTGCCAACAAACg > 1:2584374/1‑69 (MQ=255) cTTTAAGAAAGGCTGCTATACCGGACAAGAGATGGTGGCGCGAGCAAAATTCCGTGGTGCCAACAAACg > 1:1867983/1‑69 (MQ=255) | AGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGTTATACCGGACAAGAGATGGTGGCGCGAGCAAAATTCCGTGGTGCCAATAAACG > minE/1941196‑1941317 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |