Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,941,417 A→G 62.4% V279V (GTA→GTG ygfZ → predicted folate‑dependent regulatory protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,941,4170AG62.4% 44.8 / 47.1 77V279V (GTA→GTGygfZpredicted folate‑dependent regulatory protein
Reads supporting (aligned to +/- strand):  ref base A (21/8);  new base G (18/30);  total (39/38)
Fisher's exact test for biased strand distribution p-value = 4.52e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.91e-03

TGCCGGAAGCTGGTGAAGACTTAGAGCTGAAAATGGGCGAGAACTGGCGTCGTACCGGTACGGTACTGGCTGCGGTAAAACTGGAAGATGGTCAGGTCGTGGTACAGGTCGTCATGAATAACGATATGGA  >  minE/1941353‑1941482
                                                                |                                                                 
tGCCGGAAGCTGGTGAAGACTTAGAGCTGAAAATGGGCGAGAACTGGCGTCGTACCGGTACGGTACTgg                                                               >  1:920339/1‑69 (MQ=255)
tGCCGGAAGCTGGTGAAGACTTAGAGCTGAAAATGGGCGAGAACTGGCGTCGTACCGGTACGGTACTgg                                                               >  1:766354/1‑69 (MQ=255)
tGCCGGAAGCTGGTGAAGACTTAGAGCTGAAAATGGGCGAGAACTGGCGTCGTACCGGTACGGTACTgg                                                               >  1:2992807/1‑69 (MQ=255)
tGCCGGAAGCTGGTGAAGACTTAGAGCTGAAAATGGGCGAGAACTGGCGTCGTACCGGTACGGTACTgg                                                               >  1:3104054/1‑69 (MQ=255)
tGCCGGAAGCTGGTGAAGACTTAGAGCTGAAAATGGGCGAGAACTGGCGTCGTACCGGTACGGTACTgg                                                               >  1:3121900/1‑69 (MQ=255)
tGCCGGAAGCTGGTGAAGACTTAGAGCTGAAAATGGGCGAGAACTGGCGTCGTACCGGTACGGTACTg                                                                >  1:1114958/1‑68 (MQ=255)
       aGCTGGTGAAGACTTAGAGCTGAAAATGGGCGAGAACTGGCGTCGTACCGGTACGGTGCTGGCt                                                             >  1:1218377/1‑64 (MQ=255)
       aGCTGGTGAAGACTTAGAGCTGAAAATGGGCGAGAACTGGCGTCGTACCGGTACGGTGCTGGCTACgg                                                         >  1:3258181/1‑68 (MQ=255)
       aGCTGGTGAAGACTTAGAGCTGAAAATGGGCGAGAACTGGCGTCGTACCGGTACGGTGCTGGCTACgg                                                         >  1:1920340/1‑68 (MQ=255)
       aGCTGGTGAAGACTTAGAGCTGAAAATGGGCGAGAACTGGCGTCGTACCGGTACGGTGCTGGCTACgg                                                         >  1:2219805/1‑68 (MQ=255)
       aGCTGGTGAAGACTTAGAGCTGAAAATGGGCGAGAACTGGCGTCGTACCGGTACGGTGCTGGCTACgg                                                         >  1:2328828/1‑68 (MQ=255)
       aGCTGGTGAAGACTTAGAGCTGAAAATGGGCGAGAACTGGCGTCGTACCGGTACGGTGCTGGCTACgg                                                         >  1:2349576/1‑68 (MQ=255)
       aGCTGGTGAAGACTTAGAGCTGAAAATGGGCGAGAACTGGCGTCGTACCGGTACGGTGCTGGCTACgg                                                         >  1:2447498/1‑68 (MQ=255)
       aGCTGGTGAAGACTTAGAGCTGAAAATGGGCGAGAACTGGCGTCGTACCGGTACGGTGCTGGCTACgg                                                         >  1:2532499/1‑68 (MQ=255)
       aGCTGGTGAAGACTTAGAGCTGAAAATGGGCGAGAACTGGCGTCGTACCGGTACGGTGCTGGCTACgg                                                         >  1:1645231/1‑68 (MQ=255)
       aGCTGGTGAAGACTTAGAGCTGAAAATGGGCGAGAACTGGCGTCGTACCGGTACGGTGCTGGCTACgg                                                         >  1:1617341/1‑68 (MQ=255)
       aGCTGGTGAAGACTTAGAGCTGAAAATGGGCGAGAACTGGCGTCGTACCGGTACGGTGCTGGCTACgg                                                         >  1:1434092/1‑68 (MQ=255)
       aGCTGGTGAAGACTTAGAGCTGAAAATGGGCGAGAACTGGCGTCGTACCGGTACGGTGCTGGCTACgg                                                         >  1:1320367/1‑68 (MQ=255)
       aGCTGGTGAAGACTTAGAGCTGAAAATGGGCGAGAACTGGCGTCGTACCGGTACGGTGCTGGCTACgg                                                         >  1:504753/1‑68 (MQ=255)
       aGCTGGTGAAGACTTAGAGCTGAAAATGGGCGAGAACTGGCGTCGTACCGGTACGGTGCTGGCTACgg                                                         >  1:1167031/1‑68 (MQ=255)
       aGCTGGTGAAGACTTAGAGCTGAAAATGGGCGAGAACTGGCGTCGTACCGGTACGGTGCTGGCTACgg                                                         >  1:836894/1‑68 (MQ=255)
       aGCTGGTGAAGACTTAGAGCTGAAAATGGGCGAGAACTGGCGTCGTACCGGTACGGTGCTGGCTACgg                                                         >  1:1015401/1‑68 (MQ=255)
       aGCTGGTGAAGACTTAGAGCTGAAAAAGGGCGAGAACTGGCGTCGTACCGGTACGGTGCTGGCTACgg                                                         >  1:329645/1‑68 (MQ=255)
       aGCTCGTGAAGACTTAGAGCTGAAAATGGGCGAGAACTGGCGTCGTACCGGTACGGTGCTGGCTACgg                                                         >  1:781950/1‑68 (MQ=255)
         cTGGTGAAGACTTAGAGCTGAAAATGGGCGAGAACTGGCGTCGTACCGGTACGGTACTGGCTTCGGTaa                                                      <  1:4506/69‑1 (MQ=255)
         cTGGTGAAGACTTAGAGCTGAAAATGGGCGAGAACTGGCGTCGTACCGGTACGGTACTGGCTGCGGTaa                                                      <  1:2554770/69‑1 (MQ=255)
         cTGGTGAAGACTTAGAGCTGAAAATGGGCGAGAACTGGCGTCGTACCGGTACGGTACTGGCTGCGGTaa                                                      <  1:3091377/69‑1 (MQ=255)
         cTGGTGAAGACTTAGAGCTGAAAATGGGCGAGAACTGGCGTCGTACCGGTACGGTACTGGCTGCGGTaa                                                      <  1:829513/69‑1 (MQ=255)
         cTGGTGAAGACTTAGAGCTGAAAATGGGCGAGAACTGGCGTCGTACCGGTACGGTACTGGCTGCGGTaa                                                      <  1:1420527/69‑1 (MQ=255)
         cTGGTGAAGACTTAGAGCTGAAAATGGGCGAGAACTGGCGTCGTACCGGTACGGTACTGGCTGCGGTaa                                                      <  1:1639558/69‑1 (MQ=255)
                 gACTTAGAGCTGAAAATGGGCGAGAACTGGCGTCGTACCGGTACGGTACTGGCTGCGGTAAAACTGGaa                                              <  1:886637/69‑1 (MQ=255)
                 gACTTAGAGCTGAAAATGGGCGAGAACTGGCGTCGTACCGGTACGGTACTGGCTGCGGTAAAACTGGaa                                              <  1:2657853/69‑1 (MQ=255)
                  aCTTAGAGCTGAAAATGGGCGAGAACTTGCGTCGTACCGGTACGGTACTGGCTGCGGTAAAACTGGAAg                                             >  1:2767621/1‑69 (MQ=255)
                  aCTTAGAGCTGAAAATGGGCGAGAACTGGCGTCGTACCGGTACGGTACTGGCTGCGGTTAAACTGGAAg                                             >  1:631966/1‑69 (MQ=255)
                  aCTTAGAGCTGAAAATGGGCGAGAACTGGCGTCGTACCGGTACGGTACTGGCTGCGGTAAAACTGGAAg                                             >  1:972127/1‑69 (MQ=255)
                  aCTTAGAGCTGAAAATGGGCGAGAACTGGCGTCGTACCGGTACGGTACTGGCTGCGGTAAAACTGGAAg                                             >  1:1817069/1‑69 (MQ=255)
                  aCTTAGAGCTGAAAATGGGCGAGAACTGGCGTCGTACCGGTACGGTACTGGCTGCGGTAAAACTGGAAg                                             >  1:847450/1‑69 (MQ=255)
                  aCTTAGAGCTGAAAATGGGCGAGAACTGGCGTCGTACCGGTACGGTACTGGCTGCGGTAAAACTGGAAg                                             >  1:579334/1‑69 (MQ=255)
                  aCTTAGAGCTGAAAATGGGCGAGAACTGGCGTCGTACCGGTACGGTACTGGCTGCGGTAAAACTGGAAg                                             >  1:557508/1‑69 (MQ=255)
                  aCTTAGAGCTGAAAATGGGCGAGAACTGGCGTCGTACCGGTACGGTACTGGCTGCGGTAAAACTGGAAg                                             >  1:464617/1‑69 (MQ=255)
                  aCTTAGAGCTGAAAATGGGCGAGAACTGGCGTCGTACCGGTACGGTACTGGCTGCGGTAAAACTGGAAg                                             >  1:3417180/1‑69 (MQ=255)
                  aCTTAGAGCTGAAAATGGGCGAGAACTGGCGTCGTACCGGTACGGTACTGGCTGCGGTAAAACTGGAAg                                             >  1:1004152/1‑69 (MQ=255)
                  aCTTAGAGCTGAAAATGGGCGAGAACTGGCGTCGTACCGGTACGGTACTGGCTGCGGTAAAACTGGAAg                                             >  1:2527073/1‑69 (MQ=255)
                  aCTTAGAGCTGAAAATGGGCGAGAACTGGCGTCGTACCGGTACGGTACTGGCTGCGGTAAAACTGGAAg                                             >  1:246204/1‑69 (MQ=255)
                  aCTTAGAGCTGAAAATGGGCGAGAACTGGCGTCGTACCGGTACGGTACTGGCTGCGGTAAAACTGGAAg                                             >  1:2280483/1‑69 (MQ=255)
                  aCTTAGAGCTGAAAATGGGCGAGAACTGGCGTCGTACCGGTACGGTACTGGCTGCGGTAAAACTGGAAg                                             >  1:2082670/1‑69 (MQ=255)
                  aCTTAGAGCTGAAAATGGGCGAGAACTGGCGTCGTACCGGTACGGTACTGGCTGCGGTAAAACTGGAAg                                             >  1:1759831/1‑69 (MQ=255)
                                           cTGGCGTCGTACCGGTACGGTGCTGTCTACGGTCAAACTGGAAGATGGTCAGGTTGTGGTGCAGgttgt                    <  1:519845/69‑1 (MQ=255)
                                           cTGGCGTCGTACCGGTACGGTGCTGGCTACGGTCAAACTGGAAGATGGTCAGGTTGTGGTGCAtgttgt                    <  1:1749150/69‑1 (MQ=255)
                                           cTGGCGTCGTACCGGTACGGTGCTGGCTACGGTCAAACTGGAAGATGGTCAGGTTGTGGTGCAGgttgt                    <  1:2568220/69‑1 (MQ=255)
                                           cTGGCGTCGTACCGGTACGGTGCTGGCTACGGTCAAACTGGAAGATGGTCAGGTTGTGGTGCAGgttgt                    <  1:2270857/69‑1 (MQ=255)
                                           cTGGCGTCGTACCGGTACGGTGCTGGCTACGGTCAAACTGGAAGATGGTCAGGTTGTGGTGCAGgttgt                    <  1:1365777/69‑1 (MQ=255)
                                           cTGGCGTCGTACCGGTACGGTGCTGGCTACGGTCAAACTGGAAGATGGTCAGGTTGTGGTGCAGgttgt                    <  1:2196679/69‑1 (MQ=255)
                                           cTGGCGTCGTACCGGTACGGTGCTGGCTACGGTCAAACTGGAAGATGGTCAGGTTGTGGTGCAGgttgt                    <  1:584760/69‑1 (MQ=255)
                                           cTGGCGTCGTACCGGTACGGTGCTGGCTACGGTCAAACTGGAAGATGGTCAGGTTGTGGTGCAGgttgt                    <  1:3137680/69‑1 (MQ=255)
                                           cTGGCGTCGTACCGGTACGGTGCTGGCTACGGTCAAACTGGAAGATGGTCAGGTTGTGGTGCAGgttgt                    <  1:1152911/69‑1 (MQ=255)
                                           cTGGCGTCGTACCGGTACGGTGCTGGCTACGGTCAAACTGGAAGATGGTCAGGTTGTGGTGCAGgttgt                    <  1:1055925/69‑1 (MQ=255)
                                           cTGGCGTCGTACCGGTACGGTGCTGGCTACGGTCAAACTGGAAGATGGTCAGGTTGTGGTGCAGgttgt                    <  1:3091334/69‑1 (MQ=255)
                                           cTGGCGTCGTACCGGTACGGTGCTGGCTACGGTCAAACTGGAAGATGGTCAGGTTGTGGTGCAGgttgt                    <  1:1198866/69‑1 (MQ=255)
                                           cTGGCGTCGTACCGGTACGGTGCTGGCTACGGTCAAACTGGAAGATGGTCAGGTTGTGGTGCAGgttgt                    <  1:669316/69‑1 (MQ=255)
                                           cTGGCGTCGTACCGGTACGGTGCTGGCTACGGTCAAACTGGAAGATGGTCAGGTTGTGGTGCAGgttgt                    <  1:2642188/69‑1 (MQ=255)
                                            tGGCGTCGTACCGGTACGGTGCTGGCTACGGTCAAACTGGAAGATGGTCAGGTTGTGGTGCAGgttgt                    <  1:2302118/68‑1 (MQ=255)
                                            tGGCGTCGTACCGGTACGGTGCTGGCTACGGTCAAACTGGAAGATGGTCAGGTTGTGGTGCAGgttgt                    <  1:494346/68‑1 (MQ=255)
                                            tGGCGTCGTACCGGTACGGTGCTGGCTACGGTCAAACTGGAAGATGGTCAGGTTGTGGTGCAGgttgt                    <  1:49277/68‑1 (MQ=255)
                                            tGGCGTCGTACCGGTACGGTGCTGGCTACGGTCAAACTGGAAGATGGTCAGGTTGTGGTGCAGgttgt                    <  1:918221/68‑1 (MQ=255)
                                            tGGCGTCGTACCGGTACGGTGCTGGCTACGGTCAAACTGGAAGATGGTCAGGTTGTGGTGCAGgttgt                    <  1:1342832/68‑1 (MQ=255)
                                            tGGCGTCGTACCGGTACGGTGCTGGCTACGGTCAAACTGGAAGATGGTCAGGTTGTGGTGCAGgttgt                    <  1:3423880/68‑1 (MQ=255)
                                            tGGCGTCGTACCGGTACGGTGCTGGCTACGGTCAAACTGGAAGATGGTCAGGTTGTGGTGCAGgttgt                    <  1:236939/68‑1 (MQ=255)
                                            tGGCGTCGTACCGGTACGGTGCTGGCTACGGTCAAACTGGAAGATGGTCAGGTTGTGGTGCAGgttgt                    <  1:2604550/68‑1 (MQ=255)
                                            tGGCGTCGTACCGGTACGGTGCTGGCTACGGTCAAACTGGAAGATGGTCAGGTTGTGGTGCAGgttgt                    <  1:1400513/68‑1 (MQ=255)
                                            tGGCGTCGTACCGGTACGGTGCTGGCTACGGTCAAACTGGAAGATGGTCAGGTTGTGGTGCAGgttgt                    <  1:697855/68‑1 (MQ=255)
                                            tGGCGTCGTACCGGTACGGTGCTGGCTACGGTCAAACTGGAAGATGGTCAGGTTGTGGTGCAGgttgt                    <  1:3202088/68‑1 (MQ=255)
                                            tGGCGTCGTACCGGTACGGTGCTGGCTACGGTCAAACTGGAAGATGGTCAGGTTGTGGTGCAGgttgt                    <  1:2157028/68‑1 (MQ=255)
                                            tGGCGTCGTACCGGTACGGTGCTGGCTACGGTCAAACTGGAAGATGGTCAGGTTGTGGTGCAGgttgt                    <  1:2633593/68‑1 (MQ=255)
                                            tGGCGTCGTACCGGTACGGTGCTGGCTACGGTCAAACTGGAAGATGGTCAGGTTGTGGTGCAGgttgt                    <  1:961952/68‑1 (MQ=255)
                                            tGGCGTCGTACCGGTACGGTGCTGGCTACGGTCAAACTGGAAGATGGTCAGGTTGTGGTGCAGgttgt                    <  1:610151/68‑1 (MQ=255)
                                            tGGCGTCGTACCGGTACGGTGCTGGCTACGGTCAAACTGGAAGATGGTCAGGTTGTGGTGCAGgttgt                    <  1:1772097/68‑1 (MQ=255)
                                                        ggtacggtacTGGCTGCGGTAAAACTGGAAGATGGTCAGGTCGTGGTACAGGTCGTCATGAATAACGa        <  1:3204123/68‑1 (MQ=255)
                                                        ggtacggtacTGGCTGCGGTAAAACTGGAAGATGGTCAGGTCGTGGTACAGGTCGTCATGAATAACGa        <  1:3153268/68‑1 (MQ=255)
                                                        ggtacggtacTGGCTGCGGTAAAACTGGAAGATGGTCAGGTCGTGGTACAGGTCGTCATGAATAACGa        <  1:2966869/68‑1 (MQ=255)
                                                        ggtacggtacTGGCTGCGGTAAAACTGGAAGATGGGCAGGTCGTGGTACAGGTCGTCATGAATAACGa        <  1:1100678/68‑1 (MQ=255)
                                                         gtacggtgcTGGCTACGGTCAAACTGGAAGATGGTCAGGt                                   >  1:2468555/1‑40 (MQ=25)
                                                         gtacggtgcTGGCTACGGTCAAACTGGAAGATGGTCAGGTTGTGGTGCAGTTTGTCATGAATAACGata      >  1:724736/1‑69 (MQ=255)
                                                         gtacggtgcTGGCTACGGTCAAACTGGAAGATGGTCAGGTTGTGGTGCAGGTTGTCATGAATAACGata      >  1:543725/1‑69 (MQ=255)
                                                         gtacggtgcTGGCTACGGTCAAACTGGAAGATGGTCAGGTTGTGGTGCAGGTTGTCATGAATAACGata      >  1:2379074/1‑69 (MQ=255)
                                                            cggtacTGGCTGCGGTAAAACTGGAAGATGGTCAGGTCGTTGTACAGGTCGTCATGAATAACGATATgg   <  1:1510749/69‑1 (MQ=255)
                                                            cggtacTGGCTGCGGTAAAACTGGAAGATGGTCAGGTCGTGGTACAGGTCGTCATGAATAACGATATgg   >  1:1231856/1‑69 (MQ=255)
                                                            cggtacTGGCTGCGGTAAAACTGGAAGATGGTCAGGTCGTGGTACAGGTCGTCATGAATAACGATATgg   >  1:3161361/1‑69 (MQ=255)
                                                              gtacTGGCTGCTGTAAAACTGGAAGATGGTCAGGTCGTGGTACAGGTCGTCATGAATAACGATATGGa  <  1:2663567/68‑1 (MQ=255)
                                                              gtacTGGCTGCGGTAAAACTGGAAGATGGTCAGGTCGTGGTACAGGTCGTCATGAATAACGa        <  1:1102767/62‑1 (MQ=255)
                                                              gtacTGGCTGCGGTAAAACTGGAAGATGGTCAGGTCGTGGTACAGGTCGTCATGAATAACGATATGGa  <  1:1880487/68‑1 (MQ=255)
                                                              gtacTGGCTGCGGTAAAACTGGAAGATGGTCAGGTCGTGGTACAGGTCGTCATGAATAACGATATGGa  >  1:961915/1‑68 (MQ=255)
                                                              gtacTGGCTGCGGTAAAACTGGAAGATGGTCAGGTCGTGGTACAGGTCGTCATGAATAACGATATGGa  >  1:1786310/1‑68 (MQ=255)
                                                              gtacTGGCTGCGGTAAAACTGGAAGATGGTCAGGTCGTGGTACAGGTCGTCATGAATAACGATATGGa  <  1:2040347/68‑1 (MQ=255)
                                                              gtacTGGCTGCGGTAAAACTGGAAGATGGTCAGGTCGTGGTACAGGTCGTCATGAATAACGATATGGa  >  1:1782332/1‑68 (MQ=255)
                                                              gtacTGGCTGCGGTAAAACTGGAAGATGGTCAGGTCGTGGTACAGGTCGTCATGAATAACGATATGGa  >  1:1480216/1‑68 (MQ=255)
                                                              gtacTGGCTGCGGTAAAACTGGAAGATGGTCAGGTCGTGGTACAGGTCGTCATGAATAACGATATGGa  >  1:2630163/1‑68 (MQ=255)
                                                              gtacTGGCTGCGGTAAAACTGGAAGATGGTCAGGTCGTGGTACAGGTCGTCATGAATAACAATATGGa  <  1:2468377/68‑1 (MQ=255)
                                                               tacTGGCTGCGGTAAAACTGGAAGATGGTCAGGTCGTGGTACAGGTCGTCATGAATAACGATATGGa  <  1:1736467/67‑1 (MQ=255)
                                                                |                                                                 
TGCCGGAAGCTGGTGAAGACTTAGAGCTGAAAATGGGCGAGAACTGGCGTCGTACCGGTACGGTACTGGCTGCGGTAAAACTGGAAGATGGTCAGGTCGTGGTACAGGTCGTCATGAATAACGATATGGA  >  minE/1941353‑1941482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: