| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 148,890 | C→T | 41.6% | D682D (GAC→GAT) | hrpB → | predicted ATP‑dependent helicase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 148,890 | 0 | C | T | 41.6% | 32.0 / 59.9 | 84 | D682D (GAC→GAT) | hrpB | predicted ATP‑dependent helicase |
| Reads supporting (aligned to +/- strand): ref base C (17/32); new base T (26/9); total (43/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.14e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 | |||||||||||
CTGGAAACGTGGCTGCTGCCACATATGACTGGCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGC > minE/148825‑148954 | cTGGAAACGTGGCTGCTGCCACATATGACTGGCGTACATTCACTACGCGGCCTGAAATCACTCGACAtt < 1:3388070/69‑1 (MQ=255)cTGGAAACGTGGCTGCTGCCACATATGACTGGCGTACATTCACTACGCGGCCTGAAATCACTCGACAtt < 1:2598280/69‑1 (MQ=255)cTGGAAACGTGGCTGCTGCCACATATGACTGGCGTACATTCACTACGCGGCCTGAAATCACTCGACAtt < 1:2223365/69‑1 (MQ=255)cTGGAAACGTGGCTGCTGCCACATATGACTGGCGTACATTCACTACGCGGCCTGAAATCACTCGACAtt < 1:185983/69‑1 (MQ=255)cTGGAAACGGGGCTGCTGCCACATATGACTGGCGTACATTCACTACGCGGCCTGAAATCACTCGACAtt < 1:929772/69‑1 (MQ=255) tGGAAACGTGGCTACTGCCGCATATGACAGGCGTACATTCGCTACGCGGCCTGAAATCACTCGATAttt < 1:2416856/69‑1 (MQ=255) tGGAAACGTGGCTACTGCCGCATATGACAGGCGTACATTCGCTACGCGGCCTGAAATCACTCGATAttt < 1:2757252/69‑1 (MQ=255) cGTGGCTGCTGCCACATATGACTGGCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAgg < 1:485420/69‑1 (MQ=255) cGTGGCTGCTGCCACATATGACTGGCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAgg < 1:2602550/69‑1 (MQ=255) cGTGGCTGCTGCCACATATGACTGGCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAgg < 1:2643014/69‑1 (MQ=255) cGTGGCTGCTGCCACATATGACTGGCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAgg < 1:2454004/69‑1 (MQ=255) cGTGGCTGCTGCCACATATGACTGGCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAgg < 1:393385/69‑1 (MQ=255) cGTGGCTGCTGCCACATATGACTGGCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAgg < 1:2647002/69‑1 (MQ=255) cGTGGCTGCTGCCACATATGACTGGCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAgg < 1:1262357/69‑1 (MQ=255) cGTGGCTGCTGCCACATATGACTGGCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAgg < 1:1724245/69‑1 (MQ=255) cGTGGCTGCTGCCACATATGACTGGCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAgg < 1:653384/69‑1 (MQ=255) cGTGGCTGCTGCCACATATGACTGGCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAgg < 1:1541273/69‑1 (MQ=255) cGTGGCTGCTGCCACATATGACTGGCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAgg < 1:819204/69‑1 (MQ=255) cGTGGCTGCTGCCACATATGACTGGCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAgg < 1:674579/69‑1 (MQ=255) cGTGGCTGCTGCCACATATGACTGGCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAgg < 1:2853208/69‑1 (MQ=255) cGTGGCTGCTGCCACATATGACTGGCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAgg < 1:340328/69‑1 (MQ=255) gTGGCTGCTGCCACATATGACTGGCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGc > 1:1599397/1‑69 (MQ=255) gTGGCTGCTGCCACATATGACTGGCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGc > 1:1858225/1‑69 (MQ=255) tGGCTGCTGCCACATATGACTGGCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGc > 1:2105837/1‑68 (MQ=255) tGGCTGCTGCCACATATGACTGGCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGc > 1:3211148/1‑68 (MQ=255) tGGCTGCTGCCACATATGACTGGCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGc > 1:2245390/1‑68 (MQ=255) tGGCTGCTGCCACATATGACTGGCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGc > 1:857525/1‑68 (MQ=255) tGGCTGCTGCCACATATGACTGGCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGc > 1:1681077/1‑68 (MQ=255) tatGACTGGCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTa > 1:624921/1‑67 (MQ=255) tatGACTGGCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTAc > 1:475617/1‑68 (MQ=255) tatGACTGGCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTAc > 1:1513278/1‑68 (MQ=255) tatGACTGGCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTAc > 1:704516/1‑68 (MQ=255) tatGACTGGCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTAc > 1:2909482/1‑68 (MQ=255) tatGACTGGCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTA‑‑CGGATTACTTg > 1:2262924/1‑69 (MQ=255) tGGCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTg < 1:2779072/69‑1 (MQ=255) tGGCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTg < 1:2690422/69‑1 (MQ=255) tGGCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTg < 1:3226368/69‑1 (MQ=255) tGGCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTg < 1:295239/69‑1 (MQ=255) tGGCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTg < 1:1335083/69‑1 (MQ=255) tGGCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTg < 1:1341235/69‑1 (MQ=255) tGGCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTg < 1:2461582/69‑1 (MQ=255) tGGCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTg < 1:1390568/69‑1 (MQ=255) tGGCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTg < 1:2212652/69‑1 (MQ=255) tGGCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTg < 1:796865/69‑1 (MQ=255) ggCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTg < 1:2651460/68‑1 (MQ=255) ggCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTg < 1:1915262/68‑1 (MQ=255) ggCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTg > 1:1503119/1‑68 (MQ=255) cATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGa > 1:2904961/1‑66 (MQ=255) tCACTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACtt > 1:2875209/1‑54 (MQ=255) tCACTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTg > 1:2523818/1‑55 (MQ=255) ggCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTg < 1:2707373/69‑1 (MQ=255) aaTCACTCGATATTTATCAGGCACTGCGTGGATTACTTGATTGGGTAATGCAGCAAc > 1:673008/1‑57 (MQ=255) aaTCACTCGATATTTATCAGGCACTGCGTGGATTACTTGATTGGGTAATGCAGCAAc > 1:1466616/1‑57 (MQ=255) aaTCACTCGATATTTATCAGGCACTGCGTGGATTACTTGATTGGGTAATGCAGCAAc > 1:2897989/1‑57 (MQ=255) aaTCACTCGATATTTATCAGGCACTGCGTGGATTACTTGATTGGGTAATGCAGCAACg > 1:1099266/1‑58 (MQ=255) aaTCACTCGATATTTATCAGGCACTGCGTGGATTACTTGATTGGGTAATGCAGCAACg > 1:323742/1‑58 (MQ=255) aaTCACTCGATATTTATCAGGCACTGCGTGGATTACTTGATTGGGTAATGCAGCAACg > 1:467820/1‑58 (MQ=255) aaTCACTCGATATTTATCAGGCACTGCGTGGATTACTTGATTGGGTAATGCAGCAACg > 1:3427916/1‑58 (MQ=255) aaTCACTCGATATTTATCAGGCACTGCGTGGATTACTTGATTGGGTAATGCAGCAACg > 1:447417/1‑58 (MQ=255) aaTCACTCGATATTTATCAGGCACTGCGTGGATTACTTGATTGGGTAATGCAGCAACg > 1:907407/1‑58 (MQ=255) aaTCACTCGATATTTATCAGGCACTGCGTGGATTACTTGATTGGGTAATGCAGCAACg > 1:2239662/1‑58 (MQ=255) aaTCACTCGATATTTATCAGGCACTGCGTGGATTACTTGATTGGGTAATGCAGCAACg > 1:1452912/1‑58 (MQ=255) aaTCACTCGATATTTATCAGGCACTGCGTGGATTACTTGATTGGGTAATGCAGCAACg > 1:134512/1‑58 (MQ=255) aaTCACTCGATATTTATCAGGCACTGCGTGGATTACTTGATTGGGTAATGCAGCAACg > 1:1557250/1‑58 (MQ=255) aaTCACTCGATATTTATCAGGCACTGCGTGGATTACTTGATTGGGTAATGCAGCAACg > 1:1576817/1‑58 (MQ=255) aaTCACTCGATATTTATCAGGCACTGCGTGGATTACTTGATTGGGTAATGCAGCAACg > 1:1753849/1‑58 (MQ=255) aaTCACTCGATATTTATCAGGCACTGCGTGGATTACTTGATTGGGTAATGCAGCAACg > 1:1821939/1‑58 (MQ=255) aaTCACTCGATATTTATCAGGCACTGCGTGGATTACTTGATTGGGTAATGCAGCAACg > 1:1182674/1‑58 (MQ=255) aaTCACTCGATATTTATCAGGCACTGCGTGGATTACTTGATTGGGTAATGCAGCAACg > 1:211965/1‑58 (MQ=255) aaTCACTCGATATTTATCAGGCACTGCGTGGATTACTTGATTGGGTAATGCAGCAACg > 1:1110830/1‑58 (MQ=255) aaTCACTCGATATTTATCAGGCACTGCGTGGATTACTTGATTGGGTAATGCAGCAACg > 1:2265562/1‑58 (MQ=255) aaTCACTCGATATTTATCAGGCACTGCGTGGATTACTTGATTGGGTAATGCAGCAACg > 1:1065860/1‑58 (MQ=255) aaTCACTCGATATTTATCAGGCACTGCGTGGATTACTTGATTGGGTAATGCAGCAACg > 1:2785088/1‑58 (MQ=255) aaTCACTCGATATTTATCAGGCACTGCGTGGATTACTTGATTGGGTAATGCAGCAACg > 1:2796979/1‑58 (MQ=255) aaTCACTCGATATTTATCAGGCACTGCGTGGATTACTTGATTGGGTAATGCAGCAACg > 1:2883623/1‑58 (MQ=255) aaTCACTCGATATTTATCAGGCACTGCGTGGATTACTTGATTGGGTAATGCAGCAACg > 1:3190253/1‑58 (MQ=255) aaTCACTCGATATTTATCAGGCACTGCGTGGATTACTTGATTGGGTAATACAGCAACg > 1:2790594/1‑58 (MQ=255) tCGATATTTATCAGGCACTGCGTGGATTACTTGATTGGGTAATGCAGCAACGTCTGGATAGTGAATTGc < 1:992673/69‑1 (MQ=255) tCGATATTTATCAGGCACTGCGTGGATTACTTGATTGGGTAATGCAGCAACGTCTGGATAGTGAATTGc < 1:141690/69‑1 (MQ=255) tCGATATTTATCAGGCACTGCGTGGATTACTTGATTGGGTAATGCAGCAACGTCTGGATAGTGAATTGc < 1:1535134/69‑1 (MQ=255) tCGATATTTATCAGGCACTGCGTGGATTACTTGATTGGGTAATGCAGCAACGTCTGGATAGTGAATTGc < 1:1871733/69‑1 (MQ=255) tCGATATTTATCAGGCACTGCGTGGATTACTTGATTGGGTAATGCAGCAACGTCTGGATAGTGAATTGc < 1:444617/69‑1 (MQ=255) tCGATATTTATCAGGCACTGCGTGGATTACTTGATTGGGTAATGCAGCAACGTCTGGATAGTGAATTGc < 1:2709310/69‑1 (MQ=255) tCGATATTTATCAGGCACTGCGTGGATTACTTGATTGGGTAATGCAGCAACGTCTGGATAGTGAATTGc < 1:2932262/69‑1 (MQ=255) | CTGGAAACGTGGCTGCTGCCACATATGACTGGCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGC > minE/148825‑148954 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |