| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,942,643 | T→C | 14.4% | K83K (AAA→AAG) | yqfB ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,942,643 | 0 | T | C | 14.4% | 179.8 / 15.7 | 103 | K83K (AAA→AAG) | yqfB | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (49/39); new base C (12/3); total (61/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.33e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
Only 100 of 104 total aligned reads displayed.
TTTAAAGACATTTAAATTCAATCACATAAAATTGTGTCTGACCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTGCATGTTTTTCAGTCAGCGTATCCAGCGTTA > minE/1942579‑1942710 | tttAAAGACATTTAAATTCAATCACATAAAATTGTGTCTGACCGGGATAGATGTCAGCAATGACtttt < 1:1249832/68‑1 (MQ=255) aGACATTTAAATTCAATCACATAAAATTGTGTCTGACCGGGATAGATGTCAGCAATGACTTTTttcag < 1:1792231/68‑1 (MQ=255) aGACATTTAAATTCAATCACATAAAATTGTGTCTGACCGGGATAGATGTCAGCAATGACTTTTttcag < 1:1541368/68‑1 (MQ=255) aGACATTTAAATTCAATCACATAAAATTGTGTCTGACCGGGATAGATGTCAGCAATGACTTTTttcag < 1:2795182/68‑1 (MQ=255) aGACATTTAAATTCAATCACATAAAATTGTGTCTGACCGGGATAGATGTCAGCAATGACTTTTttcag < 1:1882510/68‑1 (MQ=255) aGACATTTAAATTCAATCACATAAAATTGTGTCTGACCGGGATAGATGTCAGCAATGACTTTTttcag < 1:2387722/68‑1 (MQ=255) aGACATTTAAATTCAATCACATAAAATTGTGTCTGACCGGGATAGATGTCAGCAATGACTTTTttcag < 1:3298531/68‑1 (MQ=255) aGACATTTAAATTCAATCACATAAAATTGTGTCTGACCGGGATAGATGTCAGCAATGACTTTTttcag < 1:1506308/68‑1 (MQ=255) aGACATTTAAATTCAATCACATAAAATTGTGTCTGACCGGGATAGATGTCAGCAATGACTTTTttcag < 1:1479061/68‑1 (MQ=255) aGACATTTAAATTCAATCACATAAAATTGTGTCTGACCGGGATAGATGTCAGCAATGACTTTTttcag < 1:2175421/68‑1 (MQ=255) gACATTTAAATTCAATCACATAAAATTGTGTCTGACCGGGATAGATGTCAGCAATGACTTTTttcag < 1:3126542/67‑1 (MQ=255) gACATTTAAATTCAATCACATAAAACTGTGTCTGGTCGGGATAGATGTCGGCAATGACCTTTttcagtt < 1:3386627/69‑1 (MQ=255) gACATTTAAATTCAATCACATAAAACTGTGTCTGGTCGGGATAGATGTCGGCAATGACCTTTttcagtt < 1:1514975/69‑1 (MQ=255) aaTTCAATCACATAAAATTGTGTCTGACCGGGATAGATGTCAGCAATGACTTTTTTCAGTtcagtcag > 1:2193767/1‑68 (MQ=255) aaTTCAATCACATAAAATTGTGTCTGACCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAgg > 1:1420045/1‑69 (MQ=255) aaTTCAATCACATAAAATTGTGTCTGACCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAgg > 1:63528/1‑69 (MQ=255) aaTTCAATCACATAAAATTGTGTCTGACCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAgg > 1:689309/1‑69 (MQ=255) aaTTCAATCACATAAAATTGTGTCTGACCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAgg > 1:3394399/1‑69 (MQ=255) aaTTCAATCACATAAAATTGTGTCTGACCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAgg > 1:1927596/1‑69 (MQ=255) ttCAATCACATATAGTTGTGTCTGACCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAgg > 1:3195485/1‑67 (MQ=255) ttCAATCACATATAATTGTGTCTGACCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAgg > 1:958335/1‑67 (MQ=255) ttCAATCACATATAATTGTGTCTGACCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAgg > 1:1549728/1‑67 (MQ=255) ttCAATCACATATAATTGTGTCTGACCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAgg > 1:3301113/1‑67 (MQ=255) cAATCACATAAAATTGTGTCTGACCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCa > 1:2619222/1‑69 (MQ=255) cacaTAAAATTGTGTCTGACCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCatat > 1:2628550/1‑68 (MQ=255) cacaTAAAATTGTGTCTGACCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATAtt > 1:2435166/1‑69 (MQ=255) tGACCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTGCATg < 1:1620968/68‑1 (MQ=255) tGACCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTGCATg < 1:457908/68‑1 (MQ=255) tGACCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTGCATg < 1:616570/68‑1 (MQ=255) tGACCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTGCATg < 1:3388366/68‑1 (MQ=255) tGACCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTGCATg < 1:2965099/68‑1 (MQ=255) tGACCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTGCATg < 1:1283367/68‑1 (MQ=255) tGACCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTGCATg < 1:1084370/68‑1 (MQ=255) tGACCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTGCATGt > 1:670436/1‑69 (MQ=255) tGACCGGGATAGATATCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTGCATg < 1:1786458/68‑1 (MQ=255) gggATAGATGTCATCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTGCATGTTTTtc < 1:3324931/69‑1 (MQ=255) gggATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTGCATGTTTTtc < 1:465570/69‑1 (MQ=255) gggATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTGCATGTTTTtc < 1:2981984/69‑1 (MQ=255) gggATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTGCATGTTTTtc < 1:1508691/69‑1 (MQ=255) gggATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTGCATGTTTTtc < 1:1278576/69‑1 (MQ=255) ggATAGATGTCAGCAATGTCTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTGCATGTTTTtca < 1:1528070/69‑1 (MQ=255) ggATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTGCATGTTTTtca < 1:461091/69‑1 (MQ=255) ggATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTGCATGTTTTtc < 1:1265022/68‑1 (MQ=255) aTAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTGCATGTTTTtca > 1:3423588/1‑67 (MQ=255) aTAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTGCATGTTTTtca > 1:1717803/1‑67 (MQ=255) aTAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTGCATGTTTTtca > 1:2943087/1‑67 (MQ=255) aTAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTGCATGTTTTtca > 1:710716/1‑67 (MQ=255) gATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTGCATGTTTTtcagtcag > 1:108521/1‑69 (MQ=255) gATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTGCATGTTTTtcagtcag > 1:565135/1‑69 (MQ=255) gATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTGCATGTTTTtcagtcag > 1:62469/1‑69 (MQ=255) gATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTGCATGTTTTtcagtcag > 1:2875010/1‑69 (MQ=255) gATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTGCATGTTTTtcagtcag > 1:910385/1‑69 (MQ=255) gATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTGCATGTTTTtcagtcag > 1:2823341/1‑69 (MQ=255) gATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTGCATGTTTTtcagtcag > 1:1481381/1‑69 (MQ=255) gATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTGCATGTTTTtcagtcag > 1:1511566/1‑69 (MQ=255) gATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTGCATGTTTTtcagtcag > 1:2524396/1‑69 (MQ=255) gATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTGCATGTTTTtcagtcag > 1:1594811/1‑69 (MQ=255) tGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTGCATGTTTTTCAGTCAGCg < 1:1281423/69‑1 (MQ=255) tGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTGCATGTTTTTCAGTCAGCg < 1:2795776/69‑1 (MQ=255) tGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTGCATGTTTTTCAGTCAGCg < 1:667059/69‑1 (MQ=255) tGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTGCATGTTTTTCAGTCAGCg < 1:56876/69‑1 (MQ=255) aTGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCt > 1:2513718/1‑37 (MQ=255) aTGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCt > 1:2996609/1‑37 (MQ=255) aTGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTg > 1:1162889/1‑38 (MQ=255) aTGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTg > 1:67667/1‑38 (MQ=255) aTGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTg > 1:457319/1‑38 (MQ=255) aTGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTg > 1:2474063/1‑38 (MQ=255) aTGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTg > 1:3115314/1‑38 (MQ=255) aTGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTg > 1:649817/1‑38 (MQ=255) aTGACCTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTGCATGTTTTTCCGTCAGCGTATCCAGc > 1:583850/1‑69 (MQ=255) aTGACCTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTGCATGTTTTTCCGTCAGCGTATCCAGc > 1:587639/1‑69 (MQ=255) aTGACCTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTGCATGTTTTTCCGTCAGCGTATCCAGc > 1:602923/1‑69 (MQ=255) aTGACCTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTGCATGTTTTTCCGTCAGCGTATCCAGc > 1:666528/1‑69 (MQ=255) aTGACCTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTGCATGTTTTTCCGTCAGCGTATCCAGc > 1:1357624/1‑69 (MQ=255) aTGACCTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTGCATGTTTTTCCGTCAGCGTATCCAGc > 1:335440/1‑69 (MQ=255) aTGACCTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTGCATGTTTTTCCGTCAGCGTATCCAGc > 1:3158263/1‑69 (MQ=255) aTGACCTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTGCATGTTTTTCCGTCAGCGTATCCAGc > 1:2584427/1‑69 (MQ=255) aTGACCTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTGCATGTTTTTCCGTCAGCGTATCCAGc > 1:2905159/1‑69 (MQ=255) aTGACCTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTGCATGTTTTTCCGTCAGCGTATCCAGc > 1:924570/1‑69 (MQ=255) aTGACCTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTGCATGTTTTTCCGTCAGCGAATCCAg > 1:862669/1‑68 (MQ=255) aCTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTGCATGTTTTTCAGTCAGCg > 1:1972966/1‑58 (MQ=255) aCTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTGCATGTTTTTCAGTCAGCg > 1:1379041/1‑58 (MQ=255) aCTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTGCATGTTTTTCAGTCAGCg > 1:869539/1‑58 (MQ=255) aCTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTGCATGTTTTTCAGTCAGCg > 1:2459049/1‑58 (MQ=255) aCTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTGCATGTTTTTCAGTCAGCg > 1:1229624/1‑58 (MQ=255) aCTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTGCATGTTTTTCAGTCAGCg > 1:2901579/1‑58 (MQ=255) aCTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTGCATGTTTTTCAGTCAGCg > 1:144539/1‑58 (MQ=255) aCTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTGCATGTTTTTCAGTCAGCGTATCCAGCGt < 1:674325/68‑1 (MQ=255) aCTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTGCATGTTTTTCAGTCAGCGTATCCAGCGt < 1:1846951/68‑1 (MQ=255) aCTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTGCATGTTTTTCAGTCAGCGTATCCAGCGt < 1:389700/68‑1 (MQ=255) aCTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTGCATGTTTTTCAGTCAGCGTATCCAGCGt < 1:2080481/68‑1 (MQ=255) aCTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTGCATGTTTTTCAGTCAGCGTATCCAGCGt < 1:2084510/68‑1 (MQ=255) aCTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTGCATGTTTTTCAGTCAGCGTATCCAGCGt < 1:2419181/68‑1 (MQ=255) aCTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTGCATGTTTTTCAGTCAGCGTATCCAGCGt < 1:2495608/68‑1 (MQ=255) aCTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTGCATGTTTTTCAGTCAGCGTATCCAGCGt < 1:2758832/68‑1 (MQ=255) aCTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCAGCATGTTTTTCAGTCAGCGTATCCAGCGt < 1:1534088/68‑1 (MQ=255) aCTTTTTTCAGTTCAGTCAGGGTCATATTTACCTGCTCTGCATGTTTTTCAGTCAGCg > 1:2870990/1‑58 (MQ=255) cTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTGCATGTTTTTCAGTCAGCGTATCCAGCGTTa > 1:1758065/1‑69 (MQ=255) cTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTGCATGTTTTTCAGTCAGCGTATCCAGCGTTa > 1:1887669/1‑69 (MQ=255) cTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTGCATGTTTTTCAGTCAGCGTATCCAGCGTTa > 1:2161481/1‑69 (MQ=255) | TTTAAAGACATTTAAATTCAATCACATAAAATTGTGTCTGACCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTGCATGTTTTTCAGTCAGCGTATCCAGCGTTA > minE/1942579‑1942710 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |