| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,943,994 | A→G | 45.5% | P355P (CCA→CCG) | bglA → | 6‑phospho‑beta‑glucosidase A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,943,994 | 0 | A | G | 45.5% | 4.5 / 44.0 | 55 | P355P (CCA→CCG) | bglA | 6‑phospho‑beta‑glucosidase A |
| Reads supporting (aligned to +/- strand): ref base A (15/15); new base G (12/13); total (27/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTG > minE/1943938‑1944055 | ggCAGCGTACCAAACCCGTTTGTTAAAGCATCTGACTGGGGCTGGCAGATTGATCCAGTAGGTCTgcgc < 1:3302572/69‑1 (MQ=255)ggCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGATTGATCCAGTAGGTCTgcgc < 1:2804944/69‑1 (MQ=255)ggCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGATTGATCCAGTAGGTCTgcgc < 1:716507/69‑1 (MQ=255)ggCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGATTGATCCAGTAGGTCTgcgc < 1:1928075/69‑1 (MQ=255)ggCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGATTGATCCAGTAGGTCTgcgc < 1:394261/69‑1 (MQ=255)ggCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGATTGATCCAGTAGGTCTgcgc < 1:1526891/69‑1 (MQ=255)ggCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGATTGATCCAGTAGATCTgcgc < 1:436210/69‑1 (MQ=255) gCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGATTGATCCAGTAGGTCTgcgc < 1:2158182/68‑1 (MQ=255) gCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGATTGATCCAGTAGGTCTgcgc < 1:2586047/68‑1 (MQ=255) gCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGATTGATCCAGTAGGTCTgcgc < 1:3410632/68‑1 (MQ=255) gCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGATTGATCCAGTAGGTCTgcgc < 1:496811/68‑1 (MQ=255) aCCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACt < 1:1366614/69‑1 (MQ=255) aCCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACt < 1:569488/69‑1 (MQ=255) aCCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACt < 1:1074441/69‑1 (MQ=255) tccGTATGTTAAAGCGTCTGACTGGGGCTGGCAGATTGATCCGGTTGGTCTGCGCTACGCACTTTGCGa < 1:438135/68‑1 (MQ=255) tccGTATGTTAAAGCGTCTGACTGGGGCTGGCAGATTGATCCGGTTGGTCTGCGCTACGCACTTTGCGa < 1:3239119/68‑1 (MQ=255) tccGTATGTTAAAGCGTCTGACTGGGGCTGGCAGATTGATCCGGTTGGTCTGCGCTACGCACTTTGCGa < 1:847254/68‑1 (MQ=255) tccGTATGTTAAAGCGTCTGACTGGGGCTGGCAGATTGATCCGGTTGGTCTGCGCTACGCACTTTGCGa < 1:2677372/68‑1 (MQ=255) tccGTATGTTAAAGCGTCTGACTGGGGCTGGCAGATTGATCCGGTTGGTCTGCGCTACGCACTTTGCGa < 1:2187372/68‑1 (MQ=255) tccGTATGTTAAAGCGTCTGACTGGGGCTGGCAGATTGATCCGGTTGGTCTGCGCTACGCACTTTGCGa < 1:992903/68‑1 (MQ=255) tccGTATGTTAAAGCGTCTGACTGGGGCTGGCAGATTGATCCGGTTGGTCTGCGCTACGCACTTTGCGa < 1:1199612/68‑1 (MQ=255) tccGTATGTTAAAGCGTCTGACTGGGGCTGGCAGATTGATCCGGTTGGTCTGCGCTACGCACTTTGCGa < 1:1449904/68‑1 (MQ=255) tccGTATGTTAAAGCGTCTGACTGGGGCTGGCAGATTGATCCGGTTGGTCTGCGCTACGCACTTTGCGa < 1:2104768/68‑1 (MQ=255) tccGTATGTTAAAGCGTCTGACTGGGGCTGGCAGATTGATCCGGTTGGTCTGCGCTACGCACTTTGCGa < 1:2054752/68‑1 (MQ=255) tccGTATGTTAAAGCGTCTGACTGGGGCTGGCAGATTGATCCGGTTGGTCTGCGCTACGCACTTTGCGa < 1:2073786/68‑1 (MQ=255) tccGTATGTTAAAGCGTCTGACTGGGGCTGGCAGATTGATCCGGTTGGGCTGCGCTACGCACTTTGCGa < 1:2981268/68‑1 (MQ=255) cccGTATGTTAAAGCATCTGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACt < 1:2100564/63‑1 (MQ=255) tGTTAAAGCATCTGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGt > 1:2524906/1‑68 (MQ=255) tGTTAAAGCATCTGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGt > 1:3002375/1‑68 (MQ=255) tGTTAAAGCATCTGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGt > 1:1339520/1‑68 (MQ=255) tGTTAAAGCATCTGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGt > 1:44690/1‑68 (MQ=255) tGTTAAAGCATCTGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGt > 1:2624762/1‑68 (MQ=255) tGTTAAAGCATCTGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGt > 1:483129/1‑68 (MQ=255) tGTTAAAGCATCTGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGt > 1:1910316/1‑68 (MQ=255) ttAAAGCATCTGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATg > 1:83558/1‑69 (MQ=255) ttAAAGCATCTGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATg > 1:3253312/1‑69 (MQ=255) cTGACTGGGGCTGGCAGATTGATCCGGTTGGTCTGCGCTACGc > 1:2173487/1‑43 (MQ=37) cTGACTGGGGCTGGCAGATTGATCCGGTTGGTCTGCGCTACGCACTTTGCGAGCTGTATGAGCGtt > 1:1336362/1‑66 (MQ=255) cTGACTGGGGCTGGCAGATTGATCCGGTTGGTCTGCGCTACGCACTTTGCGAGCTGTATGAGCGtt > 1:579077/1‑66 (MQ=255) cTGACTGGGGCTGGCAGATTGATCCGGTTGGTCTGCGCTACGCACTTTGCGAGCTGTATGAGCGtt > 1:1084922/1‑66 (MQ=255) cTGACTGGGGCTGGCAGATTGATCCGGTTGGTCTGCGCTACGCACTTTGCGAGCTGTATGAGCGtt > 1:3036607/1‑66 (MQ=255) cTGACTGGGGCTGGCAGATTGATCCGGTTGGTCTGCGCTACGCACTTTGCGAGCTGTATGAGCGtt > 1:1603640/1‑66 (MQ=255) cTGACTGGGGCTGGCAGATTGATCCGGTTGGTCTGCGCTACGCACTTTGCGAGCTGTATGAGCGtt > 1:1734114/1‑66 (MQ=255) cTGACTGGGGCTGGCAGATTGATCCGGTTGGTCTGCGCTACGCACTTTGCGAGCTGTATGAGCGtt > 1:2576541/1‑66 (MQ=255) cTGACTGGGGCTGGCAGATTGATCCGGTTGGTCTGCGCTACGCACTTTGCGAGCTGTATGAGCGtt > 1:2379569/1‑66 (MQ=255) cTGACTGGGGCTGGCAGATTGATCCGGTTGGTCTGCGCTACGCACTTTGCGAGCTGTATGAGCGtt > 1:2188459/1‑66 (MQ=255) cTGACTGGGGCTGGCAGATTGATCCGGTTGGTCTGCGCTACGCACTTTGCGAGCTGTATGAGCGt > 1:21974/1‑65 (MQ=255) cTGACTGGGGCTGGCAGATTGATCCGGTTGGTCTGCGCTACGCAATTTGCGAGCTGTATGAGCGtt > 1:2189412/1‑66 (MQ=255) gCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCg > 1:201986/1‑68 (MQ=255) gCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGc > 1:3416042/1‑69 (MQ=255) gCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGc > 1:1923780/1‑69 (MQ=255) gCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGc > 1:2699655/1‑69 (MQ=255) gCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGc > 1:1244490/1‑69 (MQ=255) gCAGATTGATCCGGTTGGTCTGCGCTACGCACTTTGCGa < 1:1036783/39‑1 (MQ=37) ttGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTg > 1:2374846/1‑69 (MQ=255) | GGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTG > minE/1943938‑1944055 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |