| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,945,691 | G→A | 57.8% | D873D (GAC→GAT) | gcvP ← | glycine decarboxylase, PLP‑dependent, subunit (protein P) of glycine cleavage complex |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,945,691 | 0 | G | A | 57.8% | 16.5 / 47.5 | 64 | D873D (GAC→GAT) | gcvP | glycine decarboxylase, PLP‑dependent, subunit (protein P) of glycine cleavage complex |
| Reads supporting (aligned to +/- strand): ref base G (12/15); new base A (27/10); total (39/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.70e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
GACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCGGTTCAACCAT > minE/1945643‑1945744 | gACACCTGCTTTCACCTGATCAATTTCTGCGCGGATAGCCAGCATCGCATCGATAAAGCGATCCAGTTc > 1:5011/1‑69 (MQ=255)gACACCGGCTTTCACCTGATCAATTTCTGCGCGGATAGCCAGCATCGCATCGATAAAGCGATCCAGtt > 1:89754/1‑68 (MQ=255)gACACCGGCTTTCACCTGATCAATTTCTGCGCGGATAGCCAGCATCGCATCGATAAAGCGATCCAGtt > 1:1345689/1‑68 (MQ=255)gACACCGGCTTTCACCTGATCAATTTCTGCGCGGATAGCCAGCATCGCATCGATAAAGCGATCCAGTTc > 1:395206/1‑69 (MQ=255)gACACCGGCTTTCACCTGATCAATTTCTGCGCGGATAGCCAGCATCGCATCGATAAAGCGATCCAGTTc > 1:3286820/1‑69 (MQ=255)gACACCGGCTTTCACCTGATCAATTTCTGCGCGGATAGCCAGCATCGCATCGATAAAGCGATCCAGTTc > 1:1462431/1‑69 (MQ=255)gACACCGGCTTTCACCTGATCAATTTCTGCGCGGATAGCCAGCATCGCATCGATAAAGCGATCCAGTTc > 1:1921715/1‑69 (MQ=255)gACACCGGCTTTCACCTGATCAATTTCTGCGCGGATAGCCAGCATCGCATCGATAAAGCGATCCAGTTc > 1:3111733/1‑69 (MQ=255)gACACCGGCTTTCACCTGATCAATTTCTGCGCGGATAGCCAGCATCGCATCGATAAAGCGATCCAGTTc > 1:757890/1‑69 (MQ=255)gACACCGGCTTTCACCTGATCAATTTCTGCGCGGATAGCCAGCATCGCATCGATAAAGCGATCCAGTTc > 1:3105197/1‑69 (MQ=255)gACACCGGCTTTCACCTGATCAATTTCTGCGCGGATAGCCAGCATCGCATCGATAAAGCGATCCAGTTc > 1:910114/1‑69 (MQ=255)gACACCGGCTTTCACCTGATCAATTTCTGCGCGGATAGCCAGCATCGCATCGATAAAGCGATCCAGTTc > 1:2622445/1‑69 (MQ=255)gACACCGGCTTTCACCTGATCAATTTCTGCGCGGATAGCCAGCATCGCATCGATAAAGCGATCCAGTTc > 1:250619/1‑69 (MQ=255)gACACCGGCTTTCACCTGATCAATTTCTGAGCGGATAGCCAGCATCGCATCGATAAAGCGATCCAGTTc > 1:1843327/1‑69 (MQ=255) acacCGGCTTTCACCTGATCAATTTCTGCGCGGATAGCCAGCATCGCATCGATAAAGCGATCCAGtt > 1:1056712/1‑67 (MQ=255) acacCGGCTTTCACCTGATCAATTTCTGCGCGGATAGCCAGCATCGCATCGATAAAGCGATCCAGTTcc > 1:2662471/1‑69 (MQ=255) acacCGGCTTTCACCTGATCAATTTCTGCGCGGATAGCCAGCATCGCATCGATAAAGCGATCCAGTTcc > 1:3293108/1‑69 (MQ=255) acacCGGCTTTCACCTGATCAATTTCTGCGCGGATAGCCAGCATCGCATCGATAAAGCGATCCAGTTcc > 1:2342729/1‑69 (MQ=255) acacCGGCTTTCACCTGATCAATTTCTGCGCGGATAGCCAGCATCGCATCGATAAAGCGATCCAGTTcc > 1:1793745/1‑69 (MQ=255) acacCGGCTTTCACCTGATCAATTTCTGCGCGGATAGCCAGCATCGCATCGATAAAGCGATCCAGTTcc > 1:439607/1‑69 (MQ=255) acacCGGCTTTCACCTGATCAATTTCTGCGCGGATAGCCAGCATCGCATCGATAAAGCGATCCAGTTcc > 1:1693625/1‑69 (MQ=255) acacCGGCTTTCACCTGATCAATTTCTGCGCGGATAGCCAGCATCGCATCGATAAAGCGATCCAGTTcc > 1:615097/1‑69 (MQ=255) acacCGGCTTTCACCTGATCAATTTCTGCGCGGATAGCCAGCATCGCATCGATAAAGCGATCCAGTTcc > 1:1383584/1‑69 (MQ=255) acacCGGCTTTCACCTGATCAATTTCTGCGCGGATAGCCAGCATCGCATCGATAAAGCGATCCAGTTcc > 1:1254650/1‑69 (MQ=255) acacCGGCTTTCACCTGATCAATTTCTGCGCGGATAGCCAGCATCGCATCGATAAAGCGATCCAGTTcc > 1:864680/1‑69 (MQ=255) acacCGGCTTTCACCTGATCAATTTCTGCGCGGATAGCCAGCATCGCATCGATAAAGCGATCCAGTTcc > 1:1081375/1‑69 (MQ=255) acacCGGCTTTCACCTGATCAATTTCTGCGCGGATAGCCAGCATCGCATCGATAAAGCGATCCAGTTc > 1:1756200/1‑68 (MQ=255) cacCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCa > 1:1812163/1‑69 (MQ=255) cacCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCa > 1:2062885/1‑69 (MQ=255) ggCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTc > 1:3141436/1‑45 (MQ=255) ggCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTc > 1:936958/1‑45 (MQ=255) ggCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTc > 1:2555979/1‑45 (MQ=255) ggCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTc > 1:1062406/1‑45 (MQ=255) ggCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTc > 1:1223287/1‑45 (MQ=255) ggCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTc > 1:1317265/1‑45 (MQ=255) ggCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTc > 1:18026/1‑45 (MQ=255) ggCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTc > 1:2072443/1‑45 (MQ=255) tttCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCg < 1:2229838/37‑1 (MQ=255) tttCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCg < 1:1220393/37‑1 (MQ=255) tttCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCg < 1:512317/37‑1 (MQ=255) tttCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCg < 1:472666/37‑1 (MQ=255) tttCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCg < 1:1907504/37‑1 (MQ=255) tttCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCg < 1:3267113/37‑1 (MQ=255) tttCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCg < 1:192799/37‑1 (MQ=255) tttCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCg < 1:1943232/37‑1 (MQ=255) tttCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCg < 1:2116135/37‑1 (MQ=255) tttCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCg < 1:2615206/37‑1 (MQ=255) tttCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCg < 1:2479746/37‑1 (MQ=255) tttCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCg < 1:2528015/37‑1 (MQ=255) tCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCg < 1:2101780/35‑1 (MQ=255) tCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCg < 1:1908456/35‑1 (MQ=255) tCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAg < 1:1412772/58‑1 (MQ=255) tCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCgg > 1:252000/1‑68 (MQ=255) tCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCgg > 1:1195269/1‑68 (MQ=255) gATAGCCAGCATCGCATCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCGGTTCAACCAt < 1:2728371/69‑1 (MQ=255) gATAGCCAGCATCGCATCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCGGTTCAACCAt < 1:1681291/69‑1 (MQ=255) gATAGCCAGCATCGCATCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCGGTTCAACCAt < 1:2087464/69‑1 (MQ=255) gATAGCCAGCATCGCATCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCGGTTCAACCAt < 1:1704801/69‑1 (MQ=255) gATAGCCAGCATCGCATCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCGGTTCAACCAt < 1:3425845/69‑1 (MQ=255) gATAGCCAGCATCGCATCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCGGTTCAACCAt < 1:2024635/69‑1 (MQ=255) gATAGCCAGCATCGCATCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCGGTTCAACCAt < 1:905457/69‑1 (MQ=255) gATAGCCAGCATCGCATCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCGGTTCAACCAt < 1:1196459/69‑1 (MQ=255) gATAGCCAGCATCGCATCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCGGTTCAACCAt < 1:1966060/69‑1 (MQ=255) aTCGCATCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCGGTTCAACCAt < 1:1925251/59‑1 (MQ=255) | GACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCGGTTCAACCAT > minE/1945643‑1945744 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |