| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,950,925 | G→A | 56.9% | H221Y (CAT→TAT) | visC ← | predicted oxidoreductase, FAD/NAD(P)‑binding domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,950,925 | 0 | G | A | 56.9% | 14.9 / 47.9 | 65 | H221Y (CAT→TAT) | visC | predicted oxidoreductase, FAD/NAD(P)‑binding domain |
| Reads supporting (aligned to +/- strand): ref base G (18/10); new base A (29/8); total (47/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.67e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.08e-01 | |||||||||||
TGCTGCATCCGCTGCGCTTCCTCTGGCGACAGTGACCAGACAATCGAGCAAAGATGCGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGCC > minE/1950870‑1950983 | tgctgcATCCGCTGCGCTTCCTCTGGCGACAGTGACCAGACAATCGAGCAAAGATGCGGATCGCTAAGc > 1:660793/1‑69 (MQ=255)tgctgcATCCGCTGCGCTTCCTCTGGCGACAGTGACCAGACAATCGAGCAAAGATGCGGATCGCTAAGc > 1:1931387/1‑69 (MQ=255)tgctgcATCCGCTGCGCTTCCTCTGGCGACAGTGACCAGACAATCGAGCAAAGATGCGGATCGCTAAGc > 1:1297122/1‑69 (MQ=255)tgctgcATCCGCTGCGCTTCCTCTGGCGACAGTGACCAGACAATCGAGCAAAGATGCGGATCGCTAAGc > 1:2592673/1‑69 (MQ=255)tgctgcATCCGCTGCGCTTCCTCTGGCGACAGTGACCAGACAATCGAGCAAAGATGCGGATCGCTAAGc > 1:1778805/1‑69 (MQ=255)tgctgcATCCGCTGCGCTTCCTCTGGCGACAGTGACCAGACAATCGAGCAAAGATGCGGATCGCTAAGc > 1:256203/1‑69 (MQ=255) tgcATCCGCAGCGCTTCCTCTGGCGACAGTGACCAGACAATCGAGCAAAGATACGGATCGCTAAGCgg > 1:2129979/1‑68 (MQ=255) tgcATCCGCAGCGCTTCCTCTGGCGACAGTGACCAGACAATCGAGCAAAGATACGGATCGCTAAGCgg > 1:3327377/1‑68 (MQ=255) tgcATCCGCAGCGCTTCCTCTGGCGACAGTGACCAGACAATCGAGCAAAGATACGGATCGCTAAGCgg > 1:3162384/1‑68 (MQ=255) tgcATCCGCAGCGCTTCCTCTGGCGACAGTGACCAGACAATCGAGCAAAGATACGGATCGCTAAGCgg > 1:2385263/1‑68 (MQ=255) cGCTGCGCTTCCTCTGGCGACAGTGACCAGACAATCGAGCAAAGATGCGGATCGCTAAGCGGTaaaaa > 1:2592633/1‑68 (MQ=255) cGCTGCGCTTCCTCTGGCGACAGTGACCAGACAATCGAGCAAAGATGCGGATCGCTAAGCGGTAAAAAg > 1:2792062/1‑69 (MQ=255) cGCTGCGCTTCCTCTGGCGACAGTGACCAGACAATCGAGCAAAGATGCGGATCGCTAAGCGGTAAAAAg < 1:2158018/69‑1 (MQ=255) cGCTGCGCTTCCTCTGGCGACAGTGACCAGACAATCGAGCAAAGATGCGGATCGCTAAGCGGTAAAAAg > 1:2700004/1‑69 (MQ=255) cGCTGCGCTTCCTCTGGCGACAGTGACCAGACAATCGAGCAAAGATGCGGATCGCTAAGCGGTAAAAAg > 1:2910252/1‑69 (MQ=255) cGCTGCGCTTCCTCTGGCGACAGTGACCAGACAATCGAGCAAAGATGCGGATCGCTAAGCGGTAAAAAg < 1:3214317/69‑1 (MQ=255) cGCTGCGCTTCCTCTGGCGACAGTGACCAGACAATCGAGCAAAGATGCGGATCGCTAAGCGGTAAAAAg < 1:960592/69‑1 (MQ=255) cGCTGCGCTTCCTCTGGCGACAGTGACAAGACAATCGAGCAAAGATGCGGATCGCTAAGCGGTAAAAAg > 1:2772445/1‑69 (MQ=255) cTTCCTCTGGCGACAGTGACCAGACAATCGAGCAAAGATGCGGATCGCTAAGCGGTAAAAAGGCCAg < 1:1986146/67‑1 (MQ=255) ccTCTGGCGACAGTGCCCAGACAATCGAGCAAAGATACGGATCGCTAAGCGGTAAAAAGGCCAGAATg > 1:1695551/1‑68 (MQ=255) ccTCTGGCGACAGTGACCAGACAATCGAGCAAAGATACGGATCGCTAAGCGGTAAAAAgg > 1:2982570/1‑60 (MQ=255) ccTCTGGCGACAGTGACCAGACAATCGAGCAAAGATACGGATCGCTAAGCGGTAAAAAGGCCAGAATGc > 1:1447845/1‑69 (MQ=255) ccTCTGGCGACAGTGACCAGACAATCGAGCAAAGATACGGATCGCTAAGCGGTAAAAAGGCCAGAATGc > 1:1124164/1‑69 (MQ=255) ccTCTGGCGACAGTGACCAGACAATCGAGCAAAGATACGGATCGCTAAGCGGTAAAAAGGCCAGAATGc > 1:3058192/1‑69 (MQ=255) ccTCTGGCGACAGTGACCAGACAATCGAGCAAAGATACGGATCGCTAAGCGGTAAAAAGGCCAGAATGc > 1:3069702/1‑69 (MQ=255) ccTCTGGCGACAGTGACCAGACAATCGAGCAAAGATACGGATCGCTAAGCGGTAAAAAGGCCAGAATGc > 1:3215479/1‑69 (MQ=255) ccTCTGGCGACAGTGACCAGACAATCGAGCAAAGATACGGATCGCTAAGCGGTAAAAAGGCCAGAATGc > 1:3318781/1‑69 (MQ=255) ccTCTGGCGACAGTGACCAGACAATCGAGCAAAGATACGGATCGCTAAGCGGTAAAAAGGCCAGAATGc > 1:3386075/1‑69 (MQ=255) ccTCTGGCGACAGTGACCAGACAATCGAGCAAAGATACGGATCGCTAAGCGGTAAAAAGGCCAGAATGc > 1:489199/1‑69 (MQ=255) ccTCTGGCGACAGTGACCAGACAATCGAGCAAAGATACGGATCGCTAAGCGGTAAAAAGGCCAGAATGc > 1:562994/1‑69 (MQ=255) ccTCTGGCGACAGTGACCAGACAATCGAGCAAAGATACGGATCGCTAAGCGGTAAAAAGGCCAGAATGc > 1:591905/1‑69 (MQ=255) ccTCTGGCGACAGTGACCAGACAATCGAGCAAAGATACGGATCGCTAAGCGGTAAAAAGGCCAGAATGc > 1:621228/1‑69 (MQ=255) ccTCTGGCGACAGTGACCAGACAATCGAGCAAAGATACGGATCGCTAAGCGGTAAAAAGGCCAGAATGc > 1:708024/1‑69 (MQ=255) ccTCTGGCGACAGTGACCAGACAATCGAGCAAAGATACGGATCGCTAAGCGGTAAAAAGGCCAGAATGc > 1:772615/1‑69 (MQ=255) ccTCTGGCGACAGTGACCAGACAATCGAGCAAAGATACGGATCGCTAAGCGGTAAAAAGGCCAGAATGc > 1:843432/1‑69 (MQ=255) ccTCTGGCGACAGTGACCAGACAATCGAGCAAAGATACGGATCGCTAAGCGGTAAAAAGGCCAGAATGc > 1:913404/1‑69 (MQ=255) ccTCTGGCGACAGTGACCAGACAATCGAGCAAAGATACGGATCGCTAAGCGGTAAAAAGGCCAGAATGc > 1:1518259/1‑69 (MQ=255) ccTCTGGCGACAGTGACCAGACAATCGAGCAAAGATACGGATCGCTAAGCGGTAAAAAGGCCAGAATGc > 1:2835007/1‑69 (MQ=255) ccTCTGGCGACAGTGACCAGACAATCGAGCAAAGATACGGATCGCTAAGCGGTAAAAAGGCCAGAATGc > 1:1798558/1‑69 (MQ=255) ccTCTGGCGACAGTGACCAGACAATCGAGCAAAGATACGGATCGCTAAGCGGTAAAAAGGCCAGAATGc > 1:2284973/1‑69 (MQ=255) ccTCTGGCGACAGTGACCAGACAATCGAGCAAAGATACGGATCGCTAAGCGGTAAAAAGGCCAGAATGc > 1:245024/1‑69 (MQ=255) ccTCTGGCGACAGTGACCAGACAATCGAGCAAAGATACGGATCGCTAAGCGGTAAAAAGGCCAGAATGc > 1:2173704/1‑69 (MQ=255) ccTCTGGCGACAGTGACCAGACAATCGAGCAAAGATAAGGATCGCTAAGCGGTAAAAAGGCCAGAATGc > 1:2133958/1‑69 (MQ=255) ccTCTGGCGAAAGTGACCAGACAATCGAGCAAAGATACGGATc > 1:2909037/1‑43 (MQ=38) gACAGTGACCAGACAATCGAGCAAAGATGCGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCa < 1:2820516/69‑1 (MQ=255) gACAGTGACCAGACAATCGAGCAAAGATGCGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCa < 1:1033427/69‑1 (MQ=255) gACAGTGACCAGACAATCGAGCAAAGATGCGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCa < 1:635844/69‑1 (MQ=255) gACAGTGACCAGACAATCGAGCAAAGATGCGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCa < 1:714310/69‑1 (MQ=255) gACAGTGACCAGACAATCGAGCAAAGATGCGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCa < 1:882821/69‑1 (MQ=255) gACAGTGACCAGACAATCGAGCAAAGATGCGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCa < 1:1892562/69‑1 (MQ=255) gACCAGACAATCGAGCAAAGATGCGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGaaa > 1:2239254/1‑69 (MQ=255) cGAGCAAAGATGCGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTc > 1:705289/1‑48 (MQ=255) cGAGCAAAGATGCGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCcgcg > 1:1157367/1‑68 (MQ=255) cGAGCAAAGATGCGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCgcgc > 1:483010/1‑69 (MQ=255) cGAGCAAAGATGCGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCgcgc > 1:2543301/1‑69 (MQ=255) cGAGCAAAGATGCGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCgcgc > 1:1196229/1‑69 (MQ=255) cGAGCAAAGATGCGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCgcgc > 1:1432858/1‑69 (MQ=255) gAGCAAAGATACGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGcc < 1:2101461/69‑1 (MQ=255) gAGCAAAGATACGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGcc < 1:1154773/69‑1 (MQ=255) gAGCAAAGATACGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGcc < 1:2314226/69‑1 (MQ=255) gAGCAAAGATACGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGcc < 1:577235/69‑1 (MQ=255) gAGCAAAGATACGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGcc < 1:2381700/69‑1 (MQ=255) gAGCAAAGATACGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGcc < 1:182218/69‑1 (MQ=255) gAGCAAAGATACGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGcc < 1:3257558/69‑1 (MQ=255) gAGCAAAGATACGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGcc < 1:178531/69‑1 (MQ=255) | TGCTGCATCCGCTGCGCTTCCTCTGGCGACAGTGACCAGACAATCGAGCAAAGATGCGGATCGCTAAGCGGTAAAAAGGCCAGAATGCCTTCGCCATGGAAAACCTGCCGCGCC > minE/1950870‑1950983 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |