| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,951,544 | C→G | 75.0% | V14V (GTG→GTC) | visC ← | predicted oxidoreductase, FAD/NAD(P)‑binding domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,951,544 | 0 | C | G | 75.0% | 72.7 / 22.7 | 48 | V14V (GTG→GTC) | visC | predicted oxidoreductase, FAD/NAD(P)‑binding domain |
| Reads supporting (aligned to +/- strand): ref base C (8/4); new base G (20/16); total (28/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.37e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 | |||||||||||
CGCGCTGCTCCAGTACGGCAACGCGTAAGCCGCTCCCCTGTAAGCCACAGGCAACCGCCAGCCCCACCATGCCGCCGCCAACAATGGCTACATCAACACTTTGCATTGTTTATTCCT > minE/1951480‑1951596 | cgcgCTGCTCCAGTACGGCAACGCGTAAGCCGCTCCCCTGTAAGCCACAGGCAACCGCCAGCCccac < 1:2397472/67‑1 (MQ=255) ttctCCAGTACGGCAACGCGTAAGCCGCTACCCTGTAAGCCGCAGGCAACCGCCAGCCCGACCATgccg > 1:3425446/3‑69 (MQ=255) tgctCCAGTACGGCAACGCGTAAGCCGCTACCCTGTAAGCCGCAGGCAACCGCCAGCCCGACCATgccg > 1:1962268/1‑69 (MQ=255) tgctCCAGTACGGCAACGCGTAAGCCGCTACCCTGTAAGCCGCAGGCAACCGCCAGCCCGACCATgccg > 1:2504871/1‑69 (MQ=255) tgctCCAGTACGGCAACGCGTAAGCCGCTACCCTGTAAGCCGCAGGCAACCGCCAGCCCGACCATgccg > 1:2479268/1‑69 (MQ=255) tgctCCAGTACGGCAACGCGTAAGCCGCTACCCTGTAAGCCGCAGGCAACCGCCAGCCCGACCATgccg > 1:2773700/1‑69 (MQ=255) tgctCCAGTACGGCAACGCGTAAGCCGCTACCCTGTAAGCCGCAGGCAACCGCCAGCCCGACCATgccg > 1:2873471/1‑69 (MQ=255) tgctCCAGTACGGCAACGCGTAAGCCGCTACCCTGTAAGCCGCAGGCAACCGCCAGCCCGACCATgccg > 1:2158274/1‑69 (MQ=255) tgctCCAGTACGGCAACGCGTAAGCCGCTACCCTGTAAGCCGCAGGCAACCGCCAGCCCGACCATgccg > 1:2123390/1‑69 (MQ=255) tgctCCAGTACGGCAACGCGTAAGCCGCTACCCTGTAAGCCGCAGGCAACCGCCAGCCCGACCATgccg > 1:1970088/1‑69 (MQ=255) tgctCCAGTACGGCAACGCGTAAGCCGCTACCCTGTAAGCCGCAGGCAACCGCCAGCCCGACCATgccg > 1:3233705/1‑69 (MQ=255) tgctCCAGTACGGCAACGCGTAAGCCGCTACCCTGTAAGCCGCAGGCAACCGCCAGCCCGACCATgccg > 1:1385907/1‑69 (MQ=255) tgctCCAGTACGGCAACGCGTAAGCCGCTACCCTGTAAGCCGCAGGCAACCGCCAGCCCGACCATgccg > 1:459086/1‑69 (MQ=255) tgctCCAGTACGGCAACGCGTAAGCCGCTACCCTGTAAGCCGCAGGCAACCGCCAGCCCGACCATgccg > 1:510882/1‑69 (MQ=255) tgctCCAGTACGGCAACGCGTAAGCCGCTACCCTGTAAGCCGCAGGCAACCGCCAGCCCGACCATgccg > 1:880396/1‑69 (MQ=255) tgctCCAGTACGGCAACGCGTAAGCCGCTACCCTGTAAGCCGCAGGCAACCGCCAGCCCGACCATgccg > 1:1129/1‑69 (MQ=255) tgctCCAGTACGGCAACGCGTAAGCCGCTACCCTGTAAGCCGCAGGCAACCGCCAGCCCGACCATgccg > 1:1010215/1‑69 (MQ=255) tgctCCAGTACGGCAACGCGTAAGCCGCTACCCTGTAAGCCGCAGGCAACCGCCAGCCCGACCATgcc > 1:1930893/1‑68 (MQ=255) tgctCCAGTACGGCAACGCGTAAGCCGCTACCCTGTAAGCCGCAGGCAACCGCCAGCCCGACCATgcc > 1:2610397/1‑68 (MQ=255) tgctCCAGTACGGCAACGCGTAAGCCGCTACCCTGTAAGCCGCAGGCAACCGCCAGCCCGACCATgca > 1:1926565/1‑67 (MQ=255) tgctCCAGTACGGCAACCCGTAAGCCGCTACCCTGTAAGCCGCAGGCAACCGCCAGCCCGACCATgccg > 1:1283378/1‑69 (MQ=255) aCGGCAACGCGTAAGCCGCTCCCCTGTAAGCCACAGGCAACCGCCAGCCCCACCATGCCGCCGCcaaca > 1:207576/1‑69 (MQ=255) aCGGCAACGCGTAAGCCGCTCCCCTGTAAGCCACAGGCAACCGCCAGCCCCACCATGCCGCCGCcaaca > 1:1310379/1‑69 (MQ=255) aCGGCAACGCGTAAGCCGCTCCCCTGTAAGCCACAGGCAACCGCCAGCCCCACCATGCCGCCGCcaaca > 1:3211730/1‑69 (MQ=255) aCGGCAACGCGTAAGCCGCTCCCCTGTAAGCCACAGGCAACCGCCAGCCCCACCATGCCGCCGCcaaca > 1:2800938/1‑69 (MQ=255) cGCTCCCCTGTAAGCCACAGGCAACCGCCAGCCCCACCATGCCGCCGCCAACAATGGCTACATCAacac > 1:1069926/1‑69 (MQ=255) tAAGCCACAGGCAACCGCCAGCCCCACCATGCCGCCGCCAACAATGGCTACATCAACACTTTGCATTg > 1:797785/1‑68 (MQ=255) tAAGCCACAGGCAACCGCCAGCCCCACCATGCCGCCGCCAACAATGGCTACATCAACACTTTGCATTg > 1:2280320/1‑68 (MQ=255) ccgcaGGCAACCGCCAGCCCGACCATGCCGCCGCCAACAATGGCTACATCAACACTTTGCATTGTTTAt < 1:2481321/66‑1 (MQ=255) ccgcaGGCAACCGCCAGCCCGACCATGCCGCCGCCAACAATGGCTACATCAACACTTTGCATTGTTTAt < 1:862679/66‑1 (MQ=255) ccgcaGGCAACCGCCAGCCCGACCATGCCGCCGCCAACAATGGCTACATCAACACTTTGCATTGTTTAt < 1:544717/66‑1 (MQ=255) ccgcaGGCAACCGCCAGCCCGACCATGCCGCCGCCAACAATGGCTACATCAACACTTTGCATTGTTTAt < 1:1164614/66‑1 (MQ=255) ccgcaGGCAACCGCCAGCCCGACCATGCCGCCGCCAACAATGGCTACATCAACACTTTGCATTGTTTAt < 1:481040/66‑1 (MQ=255) ccgcaGGCAACCGCCAGCCCGACCATGCCGCCGCCAACAATGGCTACATCAACACTTTGCATTGTTTAt < 1:1415614/66‑1 (MQ=255) ccgcaGGCAACCGCCAGCCCGACCATGCCGCCGCCAACAATGGCTACATCAACACTTTGCATTGTTTAt < 1:3356395/66‑1 (MQ=255) ccgcaGGCAACCGCCAGCCCGACCATGCCGCCGCCAACAATGGCTACATCAACACTTTGCATTGTTTAt < 1:330329/66‑1 (MQ=255) ccgcaGGCAACCGCCAGCCCGACCATGCCGCCGCCAACAATGGCTACATCAACACTTTGCATTGTTTAt < 1:3289891/66‑1 (MQ=255) ccgcaGGCAACCGCCAGCCCGACCATGCCGCCGCCAACAATGGCTACATCAACACTTTGCATTGTTTAt < 1:323384/66‑1 (MQ=255) ccgcaGGCAACCGCCAGCCCGACCATGCCGCCGCCAACAATGGCTACATCAACACTTTGCATTGTTTAt < 1:1762353/66‑1 (MQ=255) ccgcaGGCAACCGCCAGCCCGACCATGCCGCCGCCAACAATGGCTACATCAACACTTTGCATTGTTTAt < 1:2176457/66‑1 (MQ=255) ccgcaGGCAACCGCCAGCCCGACCATGCCGCCGCCAACAATGGCTACATCAACACTTTGCATTGTTTAt < 1:2311802/66‑1 (MQ=255) ccgcaGGCAACCGCCAGCCCGACCATGCCGCCGCCAACAATGGCTACATCAACACTTTGCATTGTTTAt < 1:2472760/66‑1 (MQ=255) ccgcaGGCAACCGCCAGCCCGACCATGCCGCCGCCAACAATGGCTACATCAACACTTTGCATTGTTTAt < 1:2539606/66‑1 (MQ=255) ccgcaGGCAACCGCCAGCCCGACCATGCAGCCGCCAACAATGGCTACATCAACACTTTGCATTGTTTAt < 1:1163205/66‑1 (MQ=255) ccACAGGCAACCGCCAGCCCCACCATGCCGCCGCCAACAATGGCTACATCAACACTTTGCATTGTTTAt < 1:316210/69‑1 (MQ=255) ccACAGGCAACCGCCAGCCCCACCATGCCGCCGCCAACAATGGCTACATCAACACTTTGCATTGTTTAt < 1:3049197/69‑1 (MQ=255) cacaGGCAACCGCCAGCCCCACCATGCCGCCGCCAACAATGGCTACATCAACACTTTGCATTGTTTAtt < 1:1291294/69‑1 (MQ=255) acaGGCAACCGCCAGCCCCACCATGCCGCCGCCAACAATGGCTACATCAACACTTTGCATTGTTTAtt < 1:3180407/68‑1 (MQ=255) ggCAACCGCCAGCCCCACCATGCCGCCGCCAACAATGGCTACATCAACACTTTGCATTGTTTATTCCt > 1:3319133/1‑68 (MQ=255) | CGCGCTGCTCCAGTACGGCAACGCGTAAGCCGCTCCCCTGTAAGCCACAGGCAACCGCCAGCCCCACCATGCCGCCGCCAACAATGGCTACATCAACACTTTGCATTGTTTATTCCT > minE/1951480‑1951596 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |