| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,953,017 | C→A | 69.7% | V364V (GTG→GTT) | pepP ← | proline aminopeptidase P II |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,953,017 | 0 | C | A | 69.7% | 54.1 / 36.2 | 56 | V364V (GTG→GTT) | pepP | proline aminopeptidase P II |
| Reads supporting (aligned to +/- strand): ref base C (3/14); new base A (12/27); total (15/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.13e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCTACGGTCAGTACCATGCCCGGTTCCAGAATGCGCGAGCGATCCTGACCATAAACACCCACGTCATGGACATCCAGTCCTAACCAGTGGCTAAGGCCATGCATAAAGAAAGGACGATGGGC > minE/1952958‑1953079 | tctACGGTCAGTACCATGCCCGGTTCCAGAATGCGCGAGCGATCCTGACCATAAACACCCACGTCATg < 1:993092/68‑1 (MQ=255) aCCATGCCCGGTTCCAGAATGCGCGAGCGATCCTGACCATAAACACCCACGTCATGGACATCCAGTCCt < 1:1825313/69‑1 (MQ=255) ttCCAGAATGCGCGAGCGATCCTGACCATAAACACCCACGTCATGGACATCCAGTCCTAACCAGTGGCt < 1:2720012/69‑1 (MQ=255) ttCCAGAATGCGCGAGCGATCCTGACCATAAACACCCACGTCATGGACATCCAGTCCTAACCAGTGGCt < 1:2819991/69‑1 (MQ=255) gAATGCGCGAGCGATCCTGACCATAAACACCCACGTCATGGACATCCAGTCCTAACCAGTGGCTAAGGc < 1:3289229/69‑1 (MQ=255) cgaTCCTGACCATAAACACCAACGTCATGAACATCCAGTCCTAACCAGTGGCt < 1:2353903/53‑1 (MQ=255) cgaTCCTGACCATAAACACCAACGTCATGAACATCCAGTCCTAACCAGTGGCt < 1:2723597/53‑1 (MQ=255) cgaTCCTGACCATAAACACCAACGTCATGAACATCCAGTCCTAACCAGTGGCt < 1:2415683/53‑1 (MQ=255) cgaTCCTGACCATAAACACCAACGTCATGAACATCCAGTCCTAACCAGTGGCt < 1:2455479/53‑1 (MQ=255) cgaTCCTGACCATAAACACCAACGTCATGAACATCCAGTCCTAACCAGTGGCt < 1:2863611/53‑1 (MQ=255) cgaTCCTGACCATAAACACCAACGTCATGAACATCCAGTCCTAACCAGTGGCt < 1:2902930/53‑1 (MQ=255) cgaTCCTGACCATAAACACCAACGTCATGAACATCCAGTCCTAACCAGTGGCt < 1:2936452/53‑1 (MQ=255) cgaTCCTGACCATAAACACCAACGTCATGAACATCCAGTCCTAACCAGTGGCt < 1:3130585/53‑1 (MQ=255) cgaTCCTGACCATAAACACCAACGTCATGAACATCCAGTCCTAACCAGTGGCt < 1:3230472/53‑1 (MQ=255) cgaTCCTGACCATAAACACCAACGTCATGAACATCCAGTCCTAACCAGTGGCt < 1:444971/53‑1 (MQ=255) cgaTCCTGACCATAAACACCAACGTCATGAACATCCAGTCCTAACCAGTGGCt < 1:469322/53‑1 (MQ=255) cgaTCCTGACCATAAACACCAACGTCATGAACATCCAGTCCTAACCAGTGGCt < 1:499868/53‑1 (MQ=255) cgaTCCTGACCATAAACACCAACGTCATGAACATCCAGTCCTAACCAGTGGCt < 1:510156/53‑1 (MQ=255) cgaTCCTGACCATAAACACCAACGTCATGAACATCCAGTCCTAACCAGTGGCt < 1:659020/53‑1 (MQ=255) cgaTCCTGACCATAAACACCAACGTCATGAACATCCAGTCCTAACCAGTGGCt < 1:768255/53‑1 (MQ=255) cgaTCCTGACCATAAACACCAACGTCATGAACATCCAGTCCTAACCAGTGGCt < 1:933463/53‑1 (MQ=255) cgaTCCTGACCATAAACACCAACGTCATGAACATCCAGTCCTAACCAGTGGCt < 1:1430992/53‑1 (MQ=255) cgaTCCTGACCATAAACACCAACGTCATGAACATCCAGTCCTAACCAGTGGCt < 1:110443/53‑1 (MQ=255) cgaTCCTGACCATAAACACCAACGTCATGAACATCCAGTCCTAACCAGTGGCt < 1:1177762/53‑1 (MQ=255) cgaTCCTGACCATAAACACCAACGTCATGAACATCCAGTCCTAACCAGTGGCt < 1:1227694/53‑1 (MQ=255) cgaTCCTGACCATAAACACCAACGTCATGAACATCCAGTCCTAACCAGTGGCt < 1:1309713/53‑1 (MQ=255) cgaTCCTGACCATAAACACCAACGTCATGAACATCCAGTCCTAACCAGTGGCt < 1:1347343/53‑1 (MQ=255) cgaTCCTGACCATAAACACCAACGTCATGAACATCCAGTCCTAACCAGTGGCt < 1:1630512/53‑1 (MQ=255) cgaTCCTGACCATAAACACCAACGTCATGAACATCCAGTCCTAACCAGTGGCt < 1:2204999/53‑1 (MQ=255) gaTCCTGACCATAAACACCAACGTCATGAACATCCAGTCCTAACCAGTGGCt < 1:214027/52‑1 (MQ=255) gaTCCTGACCATAAACACCAACGTCATGAACATCCAGTCCTAACCAGTGGCt < 1:3329761/52‑1 (MQ=255) tCCTGATCATAAACACCAACGTCATGAACATCCAGTCCt > 1:437537/1‑39 (MQ=37) tCCTGACCATAAACACCAACGTCATGAACATCCAGTcc > 1:1419222/1‑38 (MQ=38) tCCTGACCATAAACACCAACGTCATGAACATCCAGTCCt > 1:131156/1‑39 (MQ=38) tCCTGACCATAAACACCAACGTCATGAACATCCAGTCCt > 1:2577899/1‑39 (MQ=38) tCCTGACCATAAACACCAACGTCATGAACATCCAGTCCt > 1:35529/1‑39 (MQ=38) tCCTGACCATAAACACCAACGTCATGAACATCCAGTCCt > 1:2330428/1‑39 (MQ=38) tCCTGACCATAAACACCAACGTCATGAACATCCAGTCCt > 1:525719/1‑39 (MQ=38) tCCTGACCATAAACACCAACGTCATGAACATCCAGTCCt > 1:3085831/1‑39 (MQ=38) tCCTGACCATAAACACCAACGTCATGAACATCCAGTCCt > 1:667395/1‑39 (MQ=38) tCCTGACCATAAACACCAACGTCATGAACATCCAGTCCt > 1:2928680/1‑39 (MQ=38) tCCTGACCATAAACACCAACGTCATGAACATCCAGTCCt > 1:2062719/1‑39 (MQ=38) tCCTGACCATAAACACCAAAGTCATGAACATCCAGTCCt > 1:3153358/1‑39 (MQ=25) gACCATAAACACCCACGTCATGGACATCCAGTCCTAACCAGTGGCTAAGGCCATGCATAAAGAAAGGAc < 1:714860/69‑1 (MQ=255) gACCATAAACACCCACGTCATGGACATCCAGTCCTAACCAGTGGCTAAGGCCATGCATAAAGAAAGGAc < 1:2450630/69‑1 (MQ=255) gACCATAAACACCCACGTCATGGACATCCAGTCCTAACCAGTGGCTAAGGCCATGCATAAAGAAAGGAc < 1:1490562/69‑1 (MQ=255) gACCATAAACACCCACGTCATGGACATCCAGTCCTAACCAGTGGCTAAGGCCATGCATAAAGAAAGGAc < 1:983971/69‑1 (MQ=255) gACCATAAACACCCACGTCATGGACATCCAGTCCTAACCAGTGGCTAAGGCCATGCATAAAGAAAGGAc < 1:1031935/69‑1 (MQ=255) aCCATAAACACCCACGTCATGGACATCCAGTCCTAACCAGTGGCTAAGGCCATGCa < 1:2715988/56‑1 (MQ=255) aCCATAAACACCCACGTCATGGACATCCAGTCCTAACCAGTGGCTAAGGCCATGCa < 1:1348932/56‑1 (MQ=255) aCCATAAACACCCACGTCATGGACATCCAGTCCTAACCAGTGGCTAAGGCCATGCa < 1:1291699/56‑1 (MQ=255) aCCATAAACACCCACGTCATGGACATCCAGTCCTAACCAGTGGCTAAGGCCATGCa < 1:1720019/56‑1 (MQ=255) aaCACCCACGTCATGGACATCCAGTCCTAGCCAGTGGCTAAGGCCATGCATAAAGAAAGGACGATGGGc > 1:756845/1‑69 (MQ=255) aaCACCCACGTCATGGACATCCAGTCCTAACCAGTGGCTAAGGCCATGCATAAAGAAAGGACGATGGGc > 1:2305649/1‑69 (MQ=255) aaCACCCACGTCATGGACATCCAGTCCTAACCAGTGGCTAAGGCCATGCATAAAGAAAGGACGATGGGc > 1:295500/1‑69 (MQ=255) cacCAACGTCATGAACATCCAGTCCTAACCAGTGGCt < 1:2088515/37‑1 (MQ=38) | TCTACGGTCAGTACCATGCCCGGTTCCAGAATGCGCGAGCGATCCTGACCATAAACACCCACGTCATGGACATCCAGTCCTAACCAGTGGCTAAGGCCATGCATAAAGAAAGGACGATGGGC > minE/1952958‑1953079 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |