| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,953,382 | G→A | 46.9% | L243L (CTG→TTG) | pepP ← | proline aminopeptidase P II |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,953,382 | 0 | G | A | 46.9% | 8.3 / 68.5 | 81 | L243L (CTG→TTG) | pepP | proline aminopeptidase P II |
| Reads supporting (aligned to +/- strand): ref base G (35/8); new base A (20/18); total (55/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.40e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTCACAACCCGCGTCAATCAACACCAGGTCGCCGTCGCGCATTTCACACTCGTTTTCGGTGTAGTGCAGAATGCAGCCGTTTTCACCGCTGCCGACAATGGTGTTATAGGACGGATAGCGCGCACCGTGGCG > minE/1953314‑1953445 | ttCACAACCCGCGTCAATCAACACCAGGTCGCCGTCGCGCATTTCACACTCGTTTTCGGTGTAGTGCAg < 1:1451152/69‑1 (MQ=255)ttCACAACCCGCGTCAATCAACACCAGGTCGCCGTCGCGCATTTCACACTCGTTTTCGGTGTAGTGCAg < 1:1828250/69‑1 (MQ=255)ttCACAACCCGCGTCAATCAACACCAGGTCGCCGTCGCGCATTTCACACTCGTTTTCGGTGTAGTGCAg < 1:2308587/69‑1 (MQ=255) cacaACCCGCGTCAATCAACACCAGGTCGCCGTCGCGCATTTCACACTCGTTTTCGGTGTAGTGCAGaa > 1:2968709/1‑69 (MQ=255) cacaACCCGCGTCAATCAACACCAGGTCGCCGTCGCGCATTTCACACTCGTTTTCGGTGTAGTGCAGaa > 1:1453883/1‑69 (MQ=255) caatcaaCACCAGGTCGCCGTCGCGCATTTCACACTCGTTTTCGGTGTAGTGCAGAATGCAGCCGtttt < 1:1539123/69‑1 (MQ=255) caatcaaCACCAGGTCGCCGTCGCGCATTTCACACTCGTTTTCGGTGTAGTGCAGAATGCAGCCGtttt < 1:1987675/69‑1 (MQ=255) caatcaaCACCAGGTCGCCGTCGCGCATTTCACACTCGTTTTCGGTGTAGTGCAGAATGCAGCCGtttt < 1:20576/69‑1 (MQ=255) caatcaaCACCAGGTCGCCGTCGCGCATTTCACACTCGTTTTCGGTGTAGTGCAGAATGCAGCCGtttt < 1:2132997/69‑1 (MQ=255) caatcaaCACCAGGTCGCCGTCGCGCATTTCACACTCGTTTTCGGTGTAGTGCAGAATGCAGCCGtttt < 1:714072/69‑1 (MQ=255) caatcaaCACCAGGTCGCCGTCGCGCATTTCACACTCGTTTTCGGTGTAGTGCAGAATGCAGCCGtttt < 1:2538073/69‑1 (MQ=255) caatcaaCACCAGGTCGCCGTCGCGCATTTCACACTCGTTTTCGGTGTAGTGCAGAATGCAGCCGtttt < 1:595123/69‑1 (MQ=255) caatcaaCACCAGGTCGCCGTCGCGCATTTCACACTCGTTTTCGGTGTAGTGCAGAATGCAGCCGtttt < 1:1114793/69‑1 (MQ=255) aatcaaCACCAGGTCGCCGTCGCGCATTTCACACTCGTTTTCGGTGTAGTGCAGAATGCAGCCg > 1:1800486/1‑64 (MQ=255) aatcaaCACCAGGTCGCCGTCGCGCATTTCACACTCGTTTTCGGTGTAGTGCAGAATGCAGCCGTTTTc > 1:56279/1‑69 (MQ=255) aatcaaCACCAGGTCGCCGTCGCGCATTTCACACTCGTTTTCGGTGTAGTGCAGAATGCAGCCGTTTTc > 1:289378/1‑69 (MQ=255) aatcaaCACCAGGTCGCCGTCGCGCATTTCACACTCGTTTTCGGTGTAGTGCAGAATGCAGCCGTTTTc > 1:1007134/1‑69 (MQ=255) aaCACCAGGTCGCCGTCGCGCATTTCACACTCGTTTTCGGTGTAGTGCAGAATGCAGCCGTTTTCAcc > 1:2147907/1‑68 (MQ=255) aaCACCAGGTCGCCGTCGCGCATTTCACACTCGTTTTCGGTGTAGTGCAGAATGCAGCCGTTTTCAcc > 1:2700247/1‑68 (MQ=255) aaCACCAGGTCGCCGTCGCGCATTTCACACTCGTTTTCGGTGTAGTGCAGAATGCAGCCGTTTTCAc > 1:2836099/1‑67 (MQ=255) cacCAGGTCGCCGTCGCGCATTTCACACTCGTTTTCGGTGTAGTGCAGAATGCAGCCGTTTTCACCGCt > 1:2407212/1‑69 (MQ=255) cacCAGGTCGCCGTCGCGCATTTCACACTCGTTTTCGGTGTAGTGCAGAATGCAGCCGTTTTCACCGCt > 1:1238949/1‑69 (MQ=255) cacCAGGTCGCCGTCGCGCATTTCACACTCGTTTTCGGTGTAGTGCAGAATGCAGCCGTTTTCACCGCt > 1:2918660/1‑69 (MQ=255) cacCAGGTCGCCGTCGCGCATTTCACACTCGTTTTCGGTGTAGTGCAGAATGCAGCCGTTTTCACCGCt > 1:1413795/1‑69 (MQ=255) cacCAGGTCGCCGTCGCGCATTTCACACTCGTTTTCGGTGTAGTGCAGAATGCAGCCGTTTTCACCGCt > 1:2695352/1‑69 (MQ=255) cacCAGGTCGCCGTCGCGCATTTCACACTCGTTTTCGGTGTAGTGCAGAATGCAGCCGTTTTCACCGCt > 1:3349296/1‑69 (MQ=255) aGGTCGCCGTCGCGCATTTCACACTCGTTTTCGGTGTAGTGCAGAATGCAGCCGTTTTCACCGCTGcc > 1:433681/1‑68 (MQ=255) aGGTCGCCGTCGCGCATTTCACACTCGTTTTCGGTGTAGTGCAGAATGCAGCCGTTTTCACCGCTGcc > 1:1265779/1‑68 (MQ=255) aGGTCGCCGTCGCGCATTTCACACTCGTTTTCGGTGTAGTGCAGAATGCAGCCGTTTTCACCGCTGcc > 1:1628064/1‑68 (MQ=255) aGGTCGCCGTCGCGCATTTCACACTCGTTTTCGGTGTAGTGCAGAATGCAGCCGTTTTCACCGCTGcc > 1:2541141/1‑68 (MQ=255) ggTCGCCGTCGCGCATTTCACACTCGTTTTCGGTGTAGTGCAg < 1:1998373/43‑1 (MQ=255) tCACACTCGTTTTCGGTGTAGTGCAGAATGCAGCCGTTTTCACCGCTGCCGACAATGGTGTTATAgg > 1:1975610/1‑67 (MQ=255) tCACACTCGTTTTCGGTGTAGTGCAGAATGCAGCCGTTTTCACCGCTGCCGACAATGGTGTTATAGGa > 1:3176172/1‑68 (MQ=255) tCACACTCGTTTTCGGTGTAGTGCAGAATGCAGCCGTTTTCACCGCTGCCGACAATGGTGTTATAGGa > 1:174312/1‑68 (MQ=255) tCACACTCGTTTTCGGTGTAGTGCAGAATGCAGCCGTTTTCACCGCTGCCGACAATGGTGTTATAGGa > 1:955219/1‑68 (MQ=255) tCACACTCGTTTTCGGTGTAGTGCAGAATGCAGCCGTTTTCACCGCTGCCGACAATGGTGTTATAGGa > 1:2717005/1‑68 (MQ=255) tCACACTCGTTTTCGGTGTAGTGCAGAATGAAGCCGTTTTCACCGCTGCCGACAATGGTGTTATAGGa > 1:1930093/1‑68 (MQ=255) gTTTTCGGTGTAGTGCAGAATGCAGCCGTTTTCACCGCTGCCGACAATGGTGTTATAGGACGGATAgcg > 1:819965/1‑69 (MQ=255) gTTTTCGGTGTAGTGCAGAATGCAGCCGTTTTCACCGCTGCCGACAATGGTGTTATAGGACGGATAgcg > 1:2059373/1‑69 (MQ=255) tttCGGTGTAGTGCAAAATGCATCCGTTTTCACCGCTGCcaacaa > 1:2955493/1‑45 (MQ=38) tttCGGTGTAGTGCAAAATGCATCCGTTTTCACCGCTGCCAACAATGGTGTTATAGGAAGGGTAacgcg > 1:1131719/1‑69 (MQ=255) tttCGGTGTAGTGCAAAATGCATCCGTTTTCACCGCTGCCAACAATGGTGTTATAGGAAGGGTAacgcg > 1:3405945/1‑69 (MQ=255) tttCGGTGTAGTGCAAAATGCATCCGTTTTCACCGCTGCCAACAATGGTGTTATAGGAAGGGTAacgcg > 1:3018495/1‑69 (MQ=255) tttCGGTGTAGTGCAAAATGCATCCGTTTTCACCGCTGCCAACAATGGTGTTATAGGAAGGGTAacgcg > 1:1612396/1‑69 (MQ=255) tttCGGTGTAGTGCAAAATGCATCCGTTTTCACCGCTGCCAACAATGGTGTTATAGGAAGGGTAacgcg > 1:933391/1‑69 (MQ=255) tttCGGTGTAGTGCAAAATGCATCCGTTTTCACCGCTGCCAACAATGGTGTTATAGGAAGGGTAacgcg > 1:257655/1‑69 (MQ=255) tttCGGTGTAGTGCAAAATGCATCCGTTTTCACCGCTGCCAACAATGGTGTTATAGGAAGGGTAacgcg > 1:1176788/1‑69 (MQ=255) tttCGGTGTAGTGCAAAATGCATCCGTTTTCACCGCTGCCAACAATGGTGTTATAGGAAGGGTAacgcg > 1:248489/1‑69 (MQ=255) tttCGGTGTAGTGCAAAATGCATCCGTTTTCACCGCTGCCAACAATGGTGTTATAGGAAGGGTAacgcg > 1:1723330/1‑69 (MQ=255) tttCGGTGTAGTGCAAAATGCATCCGTTTTCACCGCTGCCAACAATGGTGTTATAGGAAGGGTAacgcg > 1:1441913/1‑69 (MQ=255) tttCGGTGTAGTGCAAAATGCATCCGTTTTCACCGCTGCCAACAATGGTGTTATAGGAAGGGTAacgcg > 1:742529/1‑69 (MQ=255) tttCGGTGTAGTGCAAAATGCATCCGTTTTCACCGCTGCCAACAATGGTGTTATAGGAAGGGTAacgcg > 1:1931780/1‑69 (MQ=255) tttCGGTGTAGTGCAAAATGCATCCGTTTTCACCGCTGCCAACAATGGTGTTATAGGAAGGGTAacgcg > 1:820702/1‑69 (MQ=255) tttCGGTGTAGTGCAAAATGCATCCGTTTTCACCGCTGCCAACAATGGTGTTATAGGAAGGGTAacgcg > 1:2649791/1‑69 (MQ=255) tttCGGTGTAGTGCAAAATGCATCCGTTTTCACCGCTGCCAACAATGGTGTTATAGGAAGGGTAacgcg > 1:1981111/1‑69 (MQ=255) tttCGGTGTAGTGCAAAATGCATCCGTTTTCACCGCTGCCAACAATGGTGTTATAGGAAGGGTAacgc > 1:1393170/1‑68 (MQ=255) tttCGGTGTAGTGCAAAATGCATCCGTTTTCACCGCTGCCAACAATGGTGTTATAGGAAGGGTAacg > 1:27325/1‑67 (MQ=255) tttCGGTGTAGTGCAAAATGCATCCGTTTTCACAGCTGCCAACAATGGTGTTATAGGAAGGGTAacgcg > 1:3113413/1‑69 (MQ=255) tttCGGTGTAGTGCAAAATGAATCCGTTTTCACCGCTGCCAACAATGGTGTTATAGGAAGGGTAacgcg > 1:3267320/1‑69 (MQ=255) ttCGGTGTAGTGCAGAATGCAGCCGTTTTCACCGCTGCCGACAATGGTGTTATAGGACGGATAgcgcgc > 1:749506/1‑69 (MQ=255) tgtAGTGCAAAATGCAGCCGTTTTCACCGCTGCCAACAATGGTGTTATAGGAAGGGTAACGCGCACCGt < 1:9048/69‑1 (MQ=255) tgtAGTGCAAAATGCAGCCGTTTTCACCGCTGCCAACAATGGTGTTATAGGAAGGGTAACGCGCACCGt < 1:998633/69‑1 (MQ=255) tgtAGTGCAAAATGCAGCCGTTTTCACCGCTGCCAACAATGGTGTTATAGGAAGGGTAACGCGCACCGt < 1:636534/69‑1 (MQ=255) tgtAGTGCAAAATGCAGCCGTTTTCACCGCTGCCAACAATGGTGTTATAGGAAGGGTAACGCGCACCGt < 1:609753/69‑1 (MQ=255) tgtAGTGCAAAATGCAGCCGTTTTCACCGCTGCCAACAATGGTGTTATAGGAAGGGTAACGCGCACCGt < 1:3274039/69‑1 (MQ=255) tgtAGTGCAAAATGCAGCCGTTTTCACCGCTGCCAACAATGGTGTTATAGGAAGGGTAACGCGCACCGt < 1:2972208/69‑1 (MQ=255) tgtAGTGCAAAATGCAGCCGTTTTCACCGCTGCCAACAATGGTGTTATAGGAAGGGTAACGCGCACCGt < 1:1143734/69‑1 (MQ=255) tgtAGTGCAAAATGCAGCCGTTTTCACCGCTGCCAACAATGGTGTTATAGGAAGGGTAACGCGCACCGt < 1:2869560/69‑1 (MQ=255) tgtAGTGCAAAATGCAGCCGTTTTCACCGCTGCCAACAATGGTGTTATAGGAAGGGTAACGCGCACCGt < 1:1214321/69‑1 (MQ=255) tgtAGTGCAAAATGCAGCCGTTTTCACCGCTGCCAACAATGGTGTTATAGGAAGGGTAACGCGCACCGt < 1:2740246/69‑1 (MQ=255) tgtAGTGCAAAATGCAGCCGTTTTCACCGCTGCCAACAATGGTGTTATAGGAAGGGTAACGCGCACCGt < 1:2677608/69‑1 (MQ=255) tgtAGTGCAAAATGCAGCCGTTTTCACCGCTGCCAACAATGGTGTTATAGGAAGGGTAACGCGCACCGt < 1:2389771/69‑1 (MQ=255) tgtAGTGCAAAATGCAGCCGTTTTCACCGCTGCCAACAATGGTGTTATAGGAAGGGTAACGCGCACCGt < 1:2323047/69‑1 (MQ=255) tgtAGTGCAAAATGCAGCCGTTTTCACCGCTGCCAACAATGGTGTTATAGGAAGGGTAACGCGCACCGt < 1:2203246/69‑1 (MQ=255) tgtAGTGCAAAATGCAGCCGTTTTCACCGCTGCCAACAATGGTGTTATAGGAAGGGTAACGCGCACCGt < 1:1328254/69‑1 (MQ=255) tgtAGTGCAAAATGCAGCCGTTTTCACCGCTGCCAACAATGGTGTTATAGGAAGGGTAACGCGCACCGt < 1:1848473/69‑1 (MQ=255) tgtAGTGCAAAATGCAGCCGTTTTCACCGCTGCCAACAATGGTGTTATAGGAAGGGTAACGCGCACCGt < 1:154025/69‑1 (MQ=255) gtAGTGCAAAATGCAGCCGTTTTCACCGCTGCCAACAATGGTGTTATAGGAAGGGTAACGCGCACCGt < 1:1056719/68‑1 (MQ=255) gTGCAGAATGCAGCCGTTTTCACCGCTGCCGACAATGGTGTTATAGGACGGATAGCGCGCACCGTGGc > 1:8173/1‑68 (MQ=255) gTGCAGAATGCAGCCGTTTTCACCGCTGCCGACAATGGTGTTATAGGACGGATAGCGCGCACCGTGGCg > 1:1759660/1‑69 (MQ=255) gTGCAGAATGCAGCCGTTTTCACCGCTGCCGACAATGGTGTTATAGGACGGATAGCGCGCACCGTGGCg > 1:725433/1‑69 (MQ=255) gTGCAGAATGCAGCCGTTTTCACCGCTGCCGACAATGGTGTTATAGGACGGATAGCGCGCACCGTGGCg > 1:520699/1‑69 (MQ=255) gTGCAGAATGCAGCCGTTTTCACCGCTGCCGACAATGGTGTTATAGGACGGATAGCGCGCACCGTGGCg > 1:3798/1‑69 (MQ=255) gTGCAGAATGCAGCCGTTTTCACCGCTGCCGACAATGGTGTTATAGGACGGATAGCGCGCACCGTGGCg > 1:1115436/1‑69 (MQ=255) gTGCAGAATGCAGCCGTTTTCACCGCTGCCGACAATGGTGTTATAGGACGGATAGCGCGCACCGTGGCg > 1:3251082/1‑69 (MQ=255) | TTCACAACCCGCGTCAATCAACACCAGGTCGCCGTCGCGCATTTCACACTCGTTTTCGGTGTAGTGCAGAATGCAGCCGTTTTCACCGCTGCCGACAATGGTGTTATAGGACGGATAGCGCGCACCGTGGCG > minE/1953314‑1953445 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |