| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,956,137 | C→T | 42.4% | intergenic (+80/+309) | ygfA → / ← serA | predicted ligase/D‑3‑phosphoglycerate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,956,137 | 0 | C | T | 42.4% | 47.4 / 78.9 | 106 | intergenic (+80/+309) | ygfA/serA | predicted ligase/D‑3‑phosphoglycerate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base C (33/28); new base T (32/13); total (65/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.06e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.37e-01 | |||||||||||
Only 100 of 128 total aligned reads displayed.
CGCATCGCCCTGATTGACATCGTTGATTCTTTGACCTAATTTAGTGAGTAAGGGTAAGGGAGGATTGCTCCTCCCCTGAGACTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAGTATG > minE/1956070‑1956200 | cGCATCGCCCTGATTGACATCGTTGATTCTTTGACCTAATTTAGTGAGTAAGGGTAAGGGAGGATTGct > 1:72019/1‑69 (MQ=255)cGCATCGCCCTGATTGACATCGTTGATTCTTTGACCTAATTTAGTGAGTAAGGGTAAGGGAGGATTGct > 1:1392063/1‑69 (MQ=255) gCATCGCCCTGATTGACATCGTTGATTCTTTGACCTAATTTAGTGAGTAAGGGTAAGGGAGGATTGctc < 1:527096/69‑1 (MQ=255) gCATCGCCCTGATTGACATCGTTGATTCTTTGACCTAATTTAGTGAGTAAGGGTAAGGGAGGATTGctc < 1:2425939/69‑1 (MQ=255) gCATCGCCCTGATTGACATCGTTGATTCTTTGACCTAATTTAGTGAGTAAGGGTAAGGGAGGATTGctc < 1:235653/69‑1 (MQ=255) gCATCGCCCTGATTGACATCGTTGATTCTTTGACCTAATTTAGTGAGTAAGGGTAAGGGAGGATTGctc < 1:2317728/69‑1 (MQ=255) gCATCGCCCTGATTGACATCGTTGATTCTTTGACCTAATTTAGTGAGTAAGGGTAAGGGAGGATTGctc < 1:2204266/69‑1 (MQ=255) gCATCGCCCTGATTGACATCGTTGATTCTTTGACCTAATTTAGTGAGTAAGGGTAAGGGAGGATTGctc < 1:2151172/69‑1 (MQ=255) gCATCGCCCTGATTGACATCGTTGATTCTTTGACCTAATTTAGTGAGTAAGGGTAAGGGAGGATTGctc < 1:2034175/69‑1 (MQ=255) gCATCGCCCTGATTGACATCGTTGATTCTTTGACCTAATTTAGTGAGTAAGGGTAAGGGAGGATTGctc < 1:1892751/69‑1 (MQ=255) gCATCGCCCTGATTGACATCGTTGATTCTTTGACCTAATTTAGTGAGTAAGGGTAAGGGAGGATTGctc < 1:3095605/69‑1 (MQ=255) gCATCGCCCTGATTGACATCGTTGATTCTTTGACCTAATTTAGTGAGTAAGGGTAAGGGAGGATTGctc < 1:1593476/69‑1 (MQ=255) gCATCGCCCTGATTGACATCGTTGATTCTTTGACCTAATTTAGTGAGTAAGGGTAAGGGAGGATTGctc < 1:3343930/69‑1 (MQ=255) gCATCGCCCTGATTGACATCGTTGATTCTTTGACCTAATTTAGTGAGTAAGGGTAAGGGAGGATTGctc < 1:955846/69‑1 (MQ=255) gCATCGCCCTGATTGACATCGTTGATTCTTTGACCTAATTTAGTGAGTAAGGGTAAGGGAGGATTGctc < 1:1129459/69‑1 (MQ=255) aTCGCCCTGATTGACATCGTTGATTCTTTGACCTAATTTAGTGAGTAAGGGTAAGGGAGGATTGctcct < 1:1855479/69‑1 (MQ=255) aTCGCCCTGATTGACATCGTTGATTCTTTGACCTAATTTAGTGAGTAAGGGTAAGGGAGGATTGctcct < 1:767772/69‑1 (MQ=255) aTCGCCCTGATTGACATCGTTGATTCTTTGACCTAATTTAGTGAGTAAGGGTAAGGGAGGATTGctcct < 1:2806213/69‑1 (MQ=255) gATTGACATCGTTGATTCTTTGCCCTAATTTAGTGAGTAAGGGTAAGGGAGGATTGCTCCTCCCCTgag > 1:1979262/1‑69 (MQ=255) gATTGACATCGTTGATTCTTTGACCTAATTTAGTGAGTAAGGGTAAGGGAGGATTGCTCCTCCCCTtag > 1:2395242/1‑69 (MQ=255) gATTGACATCGTTGATTCTTTGACCTAATTTAGTGAGTAAGGGTAAGGGAGGATTGCTCCTCCCCTgag > 1:31703/1‑69 (MQ=255) gATTGACATCGTTGATTCTTTGACCTAATTTAGTGAGTAAGGGTAAGGGAGGATTGCTCCTCCCCTgag > 1:3363106/1‑69 (MQ=255) gATTGACATCGTTGATTCTTTGACCTAATTTAGTGAGTAAGGGTAAGGGAGGATTGCTCCTCCCCTgag > 1:2293049/1‑69 (MQ=255) gATTGACATCGTTGATTCTTTGACCTAATTTAGTGAGTAAGGGTAAGGGAGGATTGCTCCTCCCCTgag > 1:2328455/1‑69 (MQ=255) gATTGACATCGTTGATTCTTTGACCTAATTTAGTGAGTAAGGGTAAGGGAGGATTGCTCCTCCCCTgag > 1:2110176/1‑69 (MQ=255) gATTGACATCGTTGATTCTTTGACCTAATTTAGTGAGTAAGGGTAAGGGAGGATTGCTCCTCCCCTgag > 1:255975/1‑69 (MQ=255) gATTGACATCGTTGATTCTTTGACCTAATTTAGTGAGTAAGGGTAAGGGAGGATTGCTCCTCCCCTgag > 1:1926654/1‑69 (MQ=255) gATTGACATCGTTGATTCTTTGACCTAATTTAGTGAGTAAGGGTAAGGGAGGATTGCTCCTCCCCTgag < 1:1176102/69‑1 (MQ=255) gATTGACATCGTTGATTCTTTGACCTAATTTAGTGAGTAAGGGTAAGGGAGGATTGCTCCTCCCCTga > 1:3381536/1‑68 (MQ=255) tGACATCGTTGATTCTTTGACCTAATTTAGTGAGTAAGGGTAAGGGAGGATTGCTCCTCCCCTGAgact > 1:602477/1‑69 (MQ=255) tGACATCGTTGATTCTTTGACCTAATTTAGTGAGTAAGGGTAAGGGAGGATTGCTCCTCCCCTGAgact > 1:1737949/1‑69 (MQ=255) tGACATCGTTGATTCTTTGACCTAATTTAGTGAGTAAGGGTAAGGGAGGATTGCTCCTCCCCTGAgact > 1:461079/1‑69 (MQ=255) tGACATCGTTGATTCTTTGACCTAATTTAGTGAGTAAGGGTAAGGGAGGATTGCTCCTCCCCTGAgact > 1:2209050/1‑69 (MQ=255) cATCGTTGATTCTTTGACCTAATTTAGTGAGTAAGGGTAAGGGAGGATTGCTCCTCCCCTGAgactga < 1:139158/68‑1 (MQ=255) cATCGTTGATTCTTTGACCTAATTTAGTGAGTAAGGGTAAGGGAGGATTGCTCCTCCCCTGAgactga < 1:2319020/68‑1 (MQ=255) cATCGTTGATTCTTTGACCTAATTTAGTGAGTAAGGGTAAGGGAGGATTGCTCCTCCCCTGAgactga < 1:845511/68‑1 (MQ=255) cATCGTTGATTCTTTGACCTAATTTAGTGAGTAAGGGTAAGGGAGGATTGCTCCTCCCCTGAgactga < 1:1373058/68‑1 (MQ=255) cGTTGATTCTTTGACCTAATTTAGTGAGTAAGGGTAAGGGAGGATTGCTCCTCCCCTGAGACTGACTGt < 1:919930/69‑1 (MQ=255) cGTTGATTCTTTGACCTAATTTAGTGAGTAAGGGTAAGGGAGGATTGCTCCTCCCCTGAGACTGACTGt < 1:461285/69‑1 (MQ=255) cGTTGATTCTTTGACCTAATTTAGTGAGTAAGGGTAAGGGAGGATTGCTCCTCCCCTGAGACTGACTGt < 1:2910423/69‑1 (MQ=255) tGACCTAATTTAGTGAGTAAGGGTAAGGGAGGATTGCTCCTCCCCTGAGACTGACTGt < 1:979755/58‑1 (MQ=255) aaTTTAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACt < 1:636111/69‑1 (MQ=21) aaTTTAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACt < 1:2988063/69‑1 (MQ=21) aaTTTAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACt < 1:1947979/69‑1 (MQ=21) aaTTTAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACt < 1:3041282/69‑1 (MQ=21) aaTTTAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACt < 1:1263475/69‑1 (MQ=21) aaTTTAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACt < 1:3154489/69‑1 (MQ=21) aaTTTAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACt < 1:2732735/69‑1 (MQ=21) aaTTTAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACt < 1:753942/69‑1 (MQ=21) aaTTTAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACt < 1:1471151/69‑1 (MQ=21) aaTTTAGTGAGTAAGGGTAAGGGAGGATTGCTCCTCCCCTGAGACTGACTGTTAATAAGCGCTGAAACt < 1:918779/69‑1 (MQ=255) ttAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTtaataa > 1:224935/1‑55 (MQ=39) ttAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACtt > 1:2844873/1‑67 (MQ=21) ttAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACtt > 1:2854725/1‑67 (MQ=21) ttAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACtt > 1:64374/1‑67 (MQ=21) ttAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACtt > 1:528746/1‑67 (MQ=21) ttAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACtt > 1:256654/1‑67 (MQ=21) ttAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACtt > 1:112858/1‑67 (MQ=21) ttAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACtt > 1:1211094/1‑67 (MQ=21) ttAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACtt > 1:124112/1‑67 (MQ=21) ttAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACtt > 1:1271572/1‑67 (MQ=21) ttAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACtt > 1:152641/1‑67 (MQ=21) ttAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACtt > 1:1812320/1‑67 (MQ=21) ttAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACtt > 1:850626/1‑67 (MQ=21) ttAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACtt > 1:2392232/1‑67 (MQ=21) ttAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACtt > 1:2556405/1‑67 (MQ=21) ttAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACtt > 1:2453531/1‑67 (MQ=21) ttAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACtt > 1:2532201/1‑67 (MQ=21) ttAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTCGGAAACtt > 1:982191/1‑67 (MQ=25) ttAGTGAGTAAGGGTAAGGGAGGATTGCTCCTCCCCTGAGACTGACTGTTAATAAGCGCTGAAACtt < 1:2223254/67‑1 (MQ=255) ttAGTGAGTAAGGGTAAGGGAGGATTGCTCCTCCCCTGAGACTGACTGTTAATAAGCGCTGAAACtt < 1:539630/67‑1 (MQ=255) ttAGTGAGTAAGGGTAAGGGAGGATTGCTCCTCCCCTGAGACTGACTGTTAATAAGCGCTGAAACtt < 1:1110299/67‑1 (MQ=255) ttAGTGAGTAAGGGTAAGGCAGGATTGTTCCTCCCCTGTgactg > 1:1389105/1‑44 (MQ=255) gAGTAAGGGTAAGGGAGGATTGCTCCTCCCCTGAGACTGACTGTTAATAAGCGCTGAAACTTATGAGt < 1:3259641/68‑1 (MQ=255) gAGTAAGGGTAAGGGAGGATTGCTCCTCCCCTGAGACTGACTGTTAATAAGCGCTGAAACTTATGAGt < 1:659361/68‑1 (MQ=255) gAGTAAGGGTAAGGGAGGATTGCTCCTCCCCTGAGACTGACTGTTAATAAGCGCTGAAACTTATGAGt < 1:469255/68‑1 (MQ=255) gAGTAAGGGTAAGGGAGGATTGCTCCTCCCCTGAGACTGACTGTTAATAAGCGCTGAAACTTATGAGt < 1:2118400/68‑1 (MQ=255) aGTAAGGGTAAGGGAGGATTGCTCCTCCCCTGAGACTGACTGTTAATAAGCGCTGAAACTTATGAGt < 1:882660/67‑1 (MQ=255) ggaggaTTGCTCCTCCCCTGAGACTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAg > 1:9255/1‑69 (MQ=255) ggaggaTTGCTCCTCCCCTGAGACTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAg > 1:534675/1‑69 (MQ=255) ggaggaTTGCTCCTCCCCTGAGACTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAg > 1:2527023/1‑69 (MQ=255) ggaggaTTGCTCCTCCCCTGAGACTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAg > 1:397651/1‑69 (MQ=255) ggaggaTTGCTCCTCCCCTGAGACTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAg > 1:3024078/1‑69 (MQ=255) ggaggaTTGCTCCTCCCCTGAGACTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAg > 1:1226073/1‑69 (MQ=255) ggaggaTTGCTCCTCCCCTGAGACTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAg > 1:1474023/1‑69 (MQ=255) ggaggaTTGCTCCTCCCCTGAGACTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAg > 1:2387928/1‑69 (MQ=255) aggaTTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACTTATGAGTAACAGTACAATCAGt > 1:3367122/1‑68 (MQ=18) aggaTTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACTTATGAGTAACAGTACAATCAGt > 1:1220807/1‑68 (MQ=18) aggaTTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACTTATGAGTAACAGTACAATCAGt > 1:1414714/1‑68 (MQ=18) aggaTTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACTTATGAGTAACAGTACAATCAGt > 1:910071/1‑68 (MQ=18) aggaTTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACTTATGAGTAACAGTACAATCAGt > 1:1868031/1‑68 (MQ=18) aggaTTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACTTATGAGTAACAGTACAATCAGt > 1:2291836/1‑68 (MQ=18) aggaTTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACTTATGAGTAACAGTACAATCAGt > 1:2454470/1‑68 (MQ=18) aggaTTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACTTATGAGTAACAGTACAATCAGt > 1:2965056/1‑68 (MQ=18) gaTTGCTCCTCCCCTGAGACTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAGTatg < 1:2941549/69‑1 (MQ=255) gaTTGCTCCTCCCCTGAGACTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAGTatg < 1:1123261/69‑1 (MQ=255) gCTCCTCCCCTGAGACTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTAc > 1:1801411/1‑55 (MQ=255) gCTCCTCCCCTGAGACTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTAc > 1:1561351/1‑55 (MQ=255) gCTCCTCCCCTGAGACTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTAc > 1:2641591/1‑55 (MQ=255) gCTCCTCCCCTGAGACTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTAc > 1:2676539/1‑55 (MQ=255) | CGCATCGCCCTGATTGACATCGTTGATTCTTTGACCTAATTTAGTGAGTAAGGGTAAGGGAGGATTGCTCCTCCCCTGAGACTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAGTATG > minE/1956070‑1956200 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |