| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,956,148 | A→T | 47.4% | intergenic (+91/+298) | ygfA → / ← serA | predicted ligase/D‑3‑phosphoglycerate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,956,148 | 0 | A | T | 47.4% | 37.5 / 112.2 | 95 | intergenic (+91/+298) | ygfA/serA | predicted ligase/D‑3‑phosphoglycerate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base A (23/27); new base T (32/13); total (55/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.16e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01 | |||||||||||
Only 100 of 122 total aligned reads displayed.
GATTGACATCGTTGATTCTTTGACCTAATTTAGTGAGTAAGGGTAAGGGAGGATTGCTCCTCCCCTGAGACTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAGTATGATGACAAGTCGCATC > minE/1956081‑1956215 | gATTGACATCGTTGATTCTTTGCCCTAATTTAGTGAGTAAGGGTAAGGGAGGATTGCTCCTCCCCTgag > 1:1979262/1‑69 (MQ=255)gATTGACATCGTTGATTCTTTGACCTAATTTAGTGAGTAAGGGTAAGGGAGGATTGCTCCTCCCCTtag > 1:2395242/1‑69 (MQ=255)gATTGACATCGTTGATTCTTTGACCTAATTTAGTGAGTAAGGGTAAGGGAGGATTGCTCCTCCCCTgag > 1:2110176/1‑69 (MQ=255)gATTGACATCGTTGATTCTTTGACCTAATTTAGTGAGTAAGGGTAAGGGAGGATTGCTCCTCCCCTgag > 1:3363106/1‑69 (MQ=255)gATTGACATCGTTGATTCTTTGACCTAATTTAGTGAGTAAGGGTAAGGGAGGATTGCTCCTCCCCTgag > 1:255975/1‑69 (MQ=255)gATTGACATCGTTGATTCTTTGACCTAATTTAGTGAGTAAGGGTAAGGGAGGATTGCTCCTCCCCTgag < 1:1176102/69‑1 (MQ=255)gATTGACATCGTTGATTCTTTGACCTAATTTAGTGAGTAAGGGTAAGGGAGGATTGCTCCTCCCCTgag > 1:2293049/1‑69 (MQ=255)gATTGACATCGTTGATTCTTTGACCTAATTTAGTGAGTAAGGGTAAGGGAGGATTGCTCCTCCCCTgag > 1:2328455/1‑69 (MQ=255) tGACATCGTTGATTCTTTGACCTAATTTAGTGAGTAAGGGTAAGGGAGGATTGCTCCTCCCCTGAgact > 1:461079/1‑69 (MQ=255) tGACATCGTTGATTCTTTGACCTAATTTAGTGAGTAAGGGTAAGGGAGGATTGCTCCTCCCCTGAgact > 1:2209050/1‑69 (MQ=255) tGACATCGTTGATTCTTTGACCTAATTTAGTGAGTAAGGGTAAGGGAGGATTGCTCCTCCCCTGAgact > 1:602477/1‑69 (MQ=255) tGACATCGTTGATTCTTTGACCTAATTTAGTGAGTAAGGGTAAGGGAGGATTGCTCCTCCCCTGAgact > 1:1401840/1‑69 (MQ=255) cATCTTTGATTCTTTGACCTAATTTAGTGAGTAAGGGTAAGGGAGGATTGCTCCTCCCCTGAgactga < 1:2397082/68‑1 (MQ=255) cATCGTTGATTCTTTGACCTAATTTAGTGAGTAAGGGTAAGGGAGGATTGCTCCTCCCCTGAgactga < 1:2319020/68‑1 (MQ=255) cATCGTTGATTCTTTGACCTAATTTAGTGAGTAAGGGTAAGGGAGGATTGCTCCTCCCCTGAgactga < 1:139158/68‑1 (MQ=255) cATCGTTGATTCTTTGACCTAATTTAGTGAGTAAGGGTAAGGGAGGATTGCTCCTCCCCTGAgactga < 1:1373058/68‑1 (MQ=255) cATCGTTGATTCTTTGACCTAATTTAGTGAGTAAGGGTAAGGGAGGATTGCTCCTCCCCTGAgactga < 1:288999/68‑1 (MQ=255) cATCGTTGATTCTTTGACCTAATTTAGTGAGTAAGGGTAAGGGAGGATTGCTCCTCCCCTGAgactga < 1:845511/68‑1 (MQ=255) cATCGTTGATTCTTTGACCTAATTTAGTGAGTAAGGGTAAGGGAGGATTGCTCCTCCCCTGAgactga < 1:2506543/68‑1 (MQ=255) cATCGTTGATTCTTTGACCTAATTTAGTGAGTAAGGGTAAGGGAGGATTGCTCCTCCCCTGAgactga < 1:2563286/68‑1 (MQ=255) cGTTGATTCTTTGACCTAATTTAGTGAGTAAGGGTAAGGGAGGATTGCTCCTCCCCTGAGACTGACTGt < 1:2910423/69‑1 (MQ=255) cGTTGATTCTTTGACCTAATTTAGTGAGTAAGGGTAAGGGAGGATTGCTCCTCCCCTGAGACTGACTGt < 1:461285/69‑1 (MQ=255) cGTTGATTCTTTGACCTAATTTAGTGAGTAAGGGTAAGGGAGGATTGCTCCTCCCCTGAGACTGACTGt < 1:919930/69‑1 (MQ=255) gTTGATTCTTTGACCTAATTTAGTGAGTAAGGGTAAGGGAGGATTGCTCCTCCCCTGAGACTGACTGtt > 1:826659/1‑69 (MQ=255) tGACCTAATTTAGTGAGTAAGGGTAAGGGAGGATTGCTCCTCCCCTGAGACTGACTGt < 1:979755/58‑1 (MQ=255) aaTTTAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACt < 1:2732735/69‑1 (MQ=21) aaTTTAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACt < 1:2988063/69‑1 (MQ=21) aaTTTAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACt < 1:2219436/69‑1 (MQ=21) aaTTTAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACt < 1:2141316/69‑1 (MQ=21) aaTTTAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACt < 1:3041282/69‑1 (MQ=21) aaTTTAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACt < 1:3154489/69‑1 (MQ=21) aaTTTAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACt < 1:1263475/69‑1 (MQ=21) aaTTTAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACt < 1:753942/69‑1 (MQ=21) aaTTTAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACt < 1:654515/69‑1 (MQ=21) aaTTTAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACt < 1:636111/69‑1 (MQ=21) aaTTTAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACt < 1:1947979/69‑1 (MQ=21) aaTTTAGTGAGTAAGGGTAAGGGAGGATTGCTCCTCCCCTGAGACTGACTGTTAATAAGCGCTGAAACt < 1:918779/69‑1 (MQ=255) ttAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTtaataa > 1:224935/1‑55 (MQ=39) ttAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACtt > 1:1232773/1‑67 (MQ=21) ttAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACtt > 1:256654/1‑67 (MQ=21) ttAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACtt > 1:898190/1‑67 (MQ=21) ttAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACtt > 1:2532201/1‑67 (MQ=21) ttAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACtt > 1:2844873/1‑67 (MQ=21) ttAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACtt > 1:1211094/1‑67 (MQ=21) ttAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACtt > 1:2854725/1‑67 (MQ=21) ttAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACtt > 1:850626/1‑67 (MQ=21) ttAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACtt > 1:152641/1‑67 (MQ=21) ttAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACtt > 1:2392232/1‑67 (MQ=21) ttAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACtt > 1:1271572/1‑67 (MQ=21) ttAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACtt > 1:1812320/1‑67 (MQ=21) ttAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACtt > 1:528746/1‑67 (MQ=21) ttAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACtt > 1:231328/1‑67 (MQ=21) ttAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACtt > 1:64374/1‑67 (MQ=21) ttAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTCGGAAACtt > 1:982191/1‑67 (MQ=25) ttAGTGAGTAAGGGTAAGGGAGGATTGCTCCTCCCCTGAGACTGACTGTTAATAAGCGCTGAAACtt < 1:539630/67‑1 (MQ=255) ttAGTGAGTAAGGGTAAGGGAGGATTGCTCCTCCCCTGAGACTGACTGTTAATAAGCGCTGAAACtt < 1:1110299/67‑1 (MQ=255) ttAGTGAGTAAGGGTAAGGGAGGATTGCTCCTCCCCTGAGACTGACTGTTAATAAGCGCTGAAACtt < 1:2223254/67‑1 (MQ=255) ttAGTGAGTAAGGGTAAGGGAGGATTGCTCCTCCCCTGAGACTGACTGTTAATAAGCGCTGAAACtt < 1:1276646/67‑1 (MQ=255) ttAGTGAGTAAGGGTAAGGCAGGATTGTTCCTCCCCTGTgactg > 1:1389105/1‑44 (MQ=255) gAGTAAGGGTAAGGGAGGATTGCTCCTCCCCTGAGACTGACTGTTAATAAGCGCTGAAACTTATGAGt < 1:469255/68‑1 (MQ=255) gAGTAAGGGTAAGGGAGGATTGCTCCTCCCCTGAGACTGACTGTTAATAAGCGCTGAAACTTATGAGt < 1:3259641/68‑1 (MQ=255) ggaggaTTGCTCCTCCCCTGAGACTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAg > 1:3127275/1‑69 (MQ=255) ggaggaTTGCTCCTCCCCTGAGACTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAg > 1:534675/1‑69 (MQ=255) ggaggaTTGCTCCTCCCCTGAGACTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAg > 1:1474023/1‑69 (MQ=255) ggaggaTTGCTCCTCCCCTGAGACTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAg > 1:397651/1‑69 (MQ=255) ggaggaTTGCTCCTCCCCTGAGACTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAg > 1:1226073/1‑69 (MQ=255) ggaggaTTGCTCCTCCCCTGAGACTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAg > 1:3024078/1‑69 (MQ=255) ggaggaTTGCTCCTCCCCTGAGACTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAg > 1:2387928/1‑69 (MQ=255) ggaggaTTGCTCCTCCCCTGAGACTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAg > 1:2527023/1‑69 (MQ=255) ggaggaTTGCTCCTCCCCTGAGACTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAg > 1:939474/1‑69 (MQ=255) aggaTTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACTTATGAGTAACAGTACAATCAGt > 1:1220807/1‑68 (MQ=18) aggaTTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACTTATGAGTAACAGTACAATCAGt > 1:1868031/1‑68 (MQ=18) aggaTTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACTTATGAGTAACAGTACAATCAGt > 1:910071/1‑68 (MQ=18) aggaTTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACTTATGAGTAACAGTACAATCAGt > 1:3367122/1‑68 (MQ=18) aggaTTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACTTATGAGTAACAGTACAATCAGt > 1:1414714/1‑68 (MQ=18) aggaTTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACTTATGAGTAACAGTACAATCAGt > 1:2965056/1‑68 (MQ=18) aggaTTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACTTATGAGTAACAGTACAATCAGt > 1:2454470/1‑68 (MQ=18) aggaTTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACTTATGAGTAACAGTACAATCAGt > 1:1526160/1‑68 (MQ=18) gaTTGCTCCTCCCCTGAGACTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAGTatg < 1:1123261/69‑1 (MQ=255) gCTCCTCCCCTGAGACTGACTGTTAATAAGCGCTGa < 1:1861580/36‑1 (MQ=255) gCTCCTCCCCTGAGACTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTAc > 1:2641591/1‑55 (MQ=255) gCTCCTCCCCTGAGACTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTAc > 1:1801411/1‑55 (MQ=255) gCTCCTCCCCTGAGACTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTAc > 1:3396765/1‑55 (MQ=255) gCTCCTCCCCTGAGACTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTAc > 1:2676539/1‑55 (MQ=255) gCTCCTCCCCTGAGACTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTAc > 1:3347772/1‑55 (MQ=255) gCTCCTCCCCTGAGACTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTAc > 1:1561351/1‑55 (MQ=255) gagaCTGACTGTTAATAAGCGCTGAAACTTATGAGTAACGGTACAATCAGTATGATGACAAGTCGcatc < 1:1242522/69‑1 (MQ=255) gagaCTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAGTATGATGACAAGTCGcatc < 1:2081061/69‑1 (MQ=255) gagaCTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAGTATGATGACAAGTCGcatc < 1:1027410/69‑1 (MQ=255) gagaCTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAGTATGATGACAAGTCGcatc < 1:1096163/69‑1 (MQ=255) gagaCTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAGTATGATGACAAGTCGcatc < 1:92689/69‑1 (MQ=255) gagaCTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAGTATGATGACAAGTCGcatc < 1:2356505/69‑1 (MQ=255) gagaCTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAGTATGATGACAAGTCGcatc < 1:1111869/69‑1 (MQ=255) gagaCTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAGTATGATGACAAGTCGcatc < 1:912596/69‑1 (MQ=255) gagaCTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAGTATGATGACAAGTCGcatc < 1:2983423/69‑1 (MQ=255) gagaCTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAGTATGATGACAAGTCGcatc < 1:2999838/69‑1 (MQ=255) gagaCTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAGTATGATGACAAGTCGcatc < 1:3065415/69‑1 (MQ=255) gagaCTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAGTATGATGACAAGTCGcatc < 1:1864977/69‑1 (MQ=255) gagaCTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAGTATGATGACAAGTCGcatc < 1:520414/69‑1 (MQ=255) agaCTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTAc < 1:569138/43‑1 (MQ=255) | GATTGACATCGTTGATTCTTTGACCTAATTTAGTGAGTAAGGGTAAGGGAGGATTGCTCCTCCCCTGAGACTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAGTATGATGACAAGTCGCATC > minE/1956081‑1956215 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |