| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,956,169 | T→G | 38.4% | intergenic (+112/+277) | ygfA → / ← serA | predicted ligase/D‑3‑phosphoglycerate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,956,169 | 0 | T | G | 38.4% | 81.6 / 106.9 | 112 | intergenic (+112/+277) | ygfA/serA | predicted ligase/D‑3‑phosphoglycerate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base T (21/48); new base G (30/13); total (51/61) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.74e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.05e-01 | |||||||||||
Only 100 of 112 total aligned reads displayed.
AATTTAGTGAGTAAGGGTAAGGGAGGATTGCTCCTCCCCTGAGACTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAGTATGATGACAAGTCGCATCATAACCCTTC > minE/1956107‑1956225 | aaTTTAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACt < 1:493810/69‑1 (MQ=21)aaTTTAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACt < 1:2141316/69‑1 (MQ=21)aaTTTAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACt < 1:2219436/69‑1 (MQ=21)aaTTTAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACt < 1:2732735/69‑1 (MQ=21)aaTTTAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACt < 1:2988063/69‑1 (MQ=21)aaTTTAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACt < 1:3041282/69‑1 (MQ=21)aaTTTAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACt < 1:1471151/69‑1 (MQ=21)aaTTTAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACt < 1:3154489/69‑1 (MQ=21)aaTTTAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACt < 1:1263475/69‑1 (MQ=21)aaTTTAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACt < 1:636111/69‑1 (MQ=21)aaTTTAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACt < 1:654515/69‑1 (MQ=21)aaTTTAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACt < 1:753942/69‑1 (MQ=21)aaTTTAGTGAGTAAGGGTAAGGGAGGATTGCTCCTCCCCTGAGACTGACTGTTAATAAGCGCTGAAACt < 1:918779/69‑1 (MQ=255) ttAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACtt > 1:152641/1‑67 (MQ=21) ttAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACtt > 1:1812320/1‑67 (MQ=21) ttAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACtt > 1:898190/1‑67 (MQ=21) ttAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACtt > 1:2392232/1‑67 (MQ=21) ttAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACtt > 1:2453531/1‑67 (MQ=21) ttAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACtt > 1:2556405/1‑67 (MQ=21) ttAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACtt > 1:256654/1‑67 (MQ=21) ttAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACtt > 1:850626/1‑67 (MQ=21) ttAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACtt > 1:2844873/1‑67 (MQ=21) ttAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACtt > 1:2854725/1‑67 (MQ=21) ttAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACtt > 1:124112/1‑67 (MQ=21) ttAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACtt > 1:112858/1‑67 (MQ=21) ttAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACtt > 1:1211094/1‑67 (MQ=21) ttAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACtt > 1:528746/1‑67 (MQ=21) ttAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACtt > 1:1271572/1‑67 (MQ=21) ttAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACtt > 1:64374/1‑67 (MQ=21) ttAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACtt > 1:1232773/1‑67 (MQ=21) ttAGTGAGTAAGGGTAAGGGAGGATTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTCGGAAACtt > 1:982191/1‑67 (MQ=25) ttAGTGAGTAAGGGTAAGGGAGGATTGCTCCTCCCCTGAGACTGACTGTTAATAAGCGCTGAAACtt < 1:1276646/67‑1 (MQ=255) ttAGTGAGTAAGGGTAAGGGAGGATTGCTCCTCCCCTGAGACTGACTGTTAATAAGCGCTGAAACtt < 1:1110299/67‑1 (MQ=255) ttAGTGAGTAAGGGTAAGGGAGGATTGCTCCTCCCCTGAGACTGACTGTTAATAAGCGCTGAAACtt < 1:2223254/67‑1 (MQ=255) gAGTAAGGGTAAGGGAGGATTGCTCCTCCCCTGAGACTGACTGTTAATAAGCGCTGAAACTTATGAGt < 1:3259641/68‑1 (MQ=255) gAGTAAGGGTAAGGGAGGATTGCTCCTCCCCTGAGACTGACTGTTAATAAGCGCTGAAACTTATGAGt < 1:659361/68‑1 (MQ=255) gAGTAAGGGTAAGGGAGGATTGCTCCTCCCCTGAGACTGACTGTTAATAAGCGCTGAAACTTATGAGt < 1:2118400/68‑1 (MQ=255) gAGTAAGGGTAAGGGAGGATTGCTCCTCCCCTGAGACTGACTGTTAATAAGCGCTGAAACTTATGAGt < 1:469255/68‑1 (MQ=255) ggaggaTTGCTCCTCCCCTGAGACTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAg > 1:3024078/1‑69 (MQ=255) ggaggaTTGCTCCTCCCCTGAGACTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAg > 1:534675/1‑69 (MQ=255) ggaggaTTGCTCCTCCCCTGAGACTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAg > 1:2527023/1‑69 (MQ=255) ggaggaTTGCTCCTCCCCTGAGACTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAg > 1:2387928/1‑69 (MQ=255) ggaggaTTGCTCCTCCCCTGAGACTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAg > 1:1474023/1‑69 (MQ=255) ggaggaTTGCTCCTCCCCTGAGACTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAg > 1:3127275/1‑69 (MQ=255) ggaggaTTGCTCCTCCCCTGAGACTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAg > 1:9255/1‑69 (MQ=255) ggaggaTTGCTCCTCCCCTGAGACTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAg > 1:397651/1‑69 (MQ=255) ggaggaTTGCTCCTCCCCTGAGACTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAg > 1:939474/1‑69 (MQ=255) aggaTTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACTTATGAGTAACAGTACAATCAGt > 1:1220807/1‑68 (MQ=18) aggaTTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACTTATGAGTAACAGTACAATCAGt > 1:3010513/1‑68 (MQ=18) aggaTTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACTTATGAGTAACAGTACAATCAGt > 1:1414714/1‑68 (MQ=18) aggaTTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACTTATGAGTAACAGTACAATCAGt > 1:1526160/1‑68 (MQ=18) aggaTTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACTTATGAGTAACAGTACAATCAGt > 1:2965056/1‑68 (MQ=18) aggaTTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACTTATGAGTAACAGTACAATCAGt > 1:3367122/1‑68 (MQ=18) aggaTTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACTTATGAGTAACAGTACAATCAGt > 1:1868031/1‑68 (MQ=18) aggaTTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACTTATGAGTAACAGTACAATCAGt > 1:2291836/1‑68 (MQ=18) aggaTTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACTTATGAGTAACAGTACAATCAGt > 1:910071/1‑68 (MQ=18) aggaTTGTTCCTCCCCTGTGACTGACTGTTAATAAGCTGGGAAACTTATGAGTAACAGTACAATCAGt > 1:2454470/1‑68 (MQ=18) gaTTGCTCCTCCCCTGAGACTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAGTatg < 1:1123261/69‑1 (MQ=255) gCTCCTCCCCTGAGACTGACTGTTAATAAGCGCTGa < 1:1861580/36‑1 (MQ=255) gCTCCTCCCCTGAGACTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTAc > 1:1561351/1‑55 (MQ=255) gCTCCTCCCCTGAGACTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTAc > 1:23404/1‑55 (MQ=255) gCTCCTCCCCTGAGACTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTAc > 1:1801411/1‑55 (MQ=255) gCTCCTCCCCTGAGACTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTAc > 1:2676539/1‑55 (MQ=255) gCTCCTCCCCTGAGACTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTAc > 1:3396765/1‑55 (MQ=255) gCTCCTCCCCTGAGACTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTAc > 1:3347772/1‑55 (MQ=255) gCTCCTCCCCTGAGACTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTAc > 1:2641591/1‑55 (MQ=255) gagaCTGACTGTTAATAAGCGCTGAAACTTATGAGTAACGGTACAATCAGTATGATGACAAGTCGcatc < 1:1242522/69‑1 (MQ=255) gagaCTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAGTATGATGACAAGTCGcatc < 1:520414/69‑1 (MQ=255) gagaCTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAGTATGATGACAAGTCGcatc < 1:1111869/69‑1 (MQ=255) gagaCTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAGTATGATGACAAGTCGcatc < 1:1311678/69‑1 (MQ=255) gagaCTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAGTATGATGACAAGTCGcatc < 1:1027410/69‑1 (MQ=255) gagaCTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAGTATGATGACAAGTCGcatc < 1:912596/69‑1 (MQ=255) gagaCTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAGTATGATGACAAGTCGcatc < 1:1096163/69‑1 (MQ=255) gagaCTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAGTATGATGACAAGTCGcatc < 1:92689/69‑1 (MQ=255) gagaCTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAGTATGATGACAAGTCGcatc < 1:1864977/69‑1 (MQ=255) gagaCTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAGTATGATGACAAGTCGcatc < 1:2081061/69‑1 (MQ=255) gagaCTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAGTATGATGACAAGTCGcatc < 1:2999838/69‑1 (MQ=255) gagaCTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAGTATGATGACAAGTCGcatc < 1:2983423/69‑1 (MQ=255) gagaCTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAGTATGATGACAAGTCGcatc < 1:2356505/69‑1 (MQ=255) gagaCTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAGTATGATGACAAGTCGcatc < 1:2692273/69‑1 (MQ=255) agaCTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTAc < 1:569138/43‑1 (MQ=255) gactgTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAGTATGATGACAAGTCGCATCATAAcc < 1:3191093/69‑1 (MQ=255) gactgTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAGTATGATGACAAGTCGCATCATAAcc < 1:2020256/69‑1 (MQ=255) gactgTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAGTATGATGACAAGTCGCATCATAAcc < 1:2316443/69‑1 (MQ=255) gactgTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAGTATGATGACAAGTCGCATCATAAcc < 1:1784980/69‑1 (MQ=255) gactgTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAGTATGATGACAAGTCGCATCATAAcc < 1:1655869/69‑1 (MQ=255) gactgTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAGTATGATGACAAGTCGCATCATAAcc < 1:1558790/69‑1 (MQ=255) gactgTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAGTATGATGACAAGTCGCATCATAAcc < 1:3263826/69‑1 (MQ=255) actgTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAGTATGATGACAAGTCGCATCATAAccc > 1:1623737/1‑69 (MQ=255) actgTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAGTATGATGACAAGTCGCATCATAAccc > 1:1849123/1‑69 (MQ=255) actgTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAGTATGATGACAAGTCGCATCATAAccc > 1:2106283/1‑69 (MQ=255) actgTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAGTATGATGACAAGTCGCATCATAAccc > 1:3004091/1‑69 (MQ=255) tgTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAGTATGATGACAAGTCGCATCATAAccc < 1:1583423/67‑1 (MQ=255) gTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAGTATGATGACAAGTCGCATCATAACCCTtc < 1:634701/69‑1 (MQ=255) gTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAGTATGATGACAAGTCGCATCATAACCCTtc < 1:1204040/69‑1 (MQ=255) gTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAGTATGATGACAAGTCGCATCATAACCCTtc < 1:2560362/69‑1 (MQ=255) gTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAGTATGATGACAAGTCGCATCATAACCCTtc < 1:1778323/69‑1 (MQ=255) gTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAGTATGATGACAAGTCGCATCATAACCCTtc < 1:52832/69‑1 (MQ=255) gTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAGTATGATGACAAGTCGCATCATAACCCTtc < 1:511952/69‑1 (MQ=255) gTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAGTATGATGACAAGTCGCATCATAACCCTtc < 1:454231/69‑1 (MQ=255) | AATTTAGTGAGTAAGGGTAAGGGAGGATTGCTCCTCCCCTGAGACTGACTGTTAATAAGCGCTGAAACTTATGAGTAACAGTACAATCAGTATGATGACAAGTCGCATCATAACCCTTC > minE/1956107‑1956225 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |