| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,956,370 | C→T | 39.0% | intergenic (+313/+76) | ygfA → / ← serA | predicted ligase/D‑3‑phosphoglycerate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,956,370 | 0 | C | T | 39.0% | 31.8 / 40.1 | 64 | intergenic (+313/+76) | ygfA/serA | predicted ligase/D‑3‑phosphoglycerate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base C (14/25); new base T (12/13); total (26/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.36e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01 | |||||||||||
AACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAG > minE/1956305‑1956437 | aaCACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAAcgc > 1:3010305/1‑68 (MQ=255) aTTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAATGCGGCAACGgtgt < 1:2975225/69‑1 (MQ=255) aTTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAATGCGGCAACGgtgt < 1:1139366/69‑1 (MQ=255) aTTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAATGCGGCAACGgtgt < 1:898893/69‑1 (MQ=255) aTTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAATGCGGCAACGgtgt < 1:2796267/69‑1 (MQ=255) aTTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAATGCGGCAACGgtgt < 1:334285/69‑1 (MQ=255) aTTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAATGCGGCAACGgtgt < 1:882493/69‑1 (MQ=255) aTTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAATGCGGCAACGgtgt < 1:1646551/69‑1 (MQ=255) aTTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAATGCGGCAACGgtgt < 1:2131134/69‑1 (MQ=255) aTTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAATGCGGCAACGgtgt < 1:2729953/69‑1 (MQ=255) aTTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAATGCGGCAACGgtgt < 1:2117385/69‑1 (MQ=255) aTTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAATGCGGCAACGgtgt < 1:1867170/69‑1 (MQ=255) aTTGATCTGACTTTGATTTATTTACTGGAGCAGACTCGCAAAGTAGAATGCGCAATGCGGCAACGgtgt < 1:1369656/69‑1 (MQ=255) ttGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAATGCGGCAACGgtgt < 1:2393865/68‑1 (MQ=255) gATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGtgtg > 1:1078006/1‑67 (MQ=255) gATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTgg > 1:2194217/1‑68 (MQ=255) gATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTgg > 1:3094117/1‑68 (MQ=255) gATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTgg > 1:3181185/1‑68 (MQ=255) gATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTgg > 1:2280904/1‑68 (MQ=255) gATCTGACTTTGATTTATTTTCTGGAGCAGAATCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTgg > 1:542384/1‑68 (MQ=255) ttatttTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATaaaaa < 1:2632099/68‑1 (MQ=255) ttatttTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATaaaaa < 1:1365390/68‑1 (MQ=255) gACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTg < 1:2642915/69‑1 (MQ=255) gACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTg < 1:2938822/69‑1 (MQ=255) gACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTg < 1:3063563/69‑1 (MQ=255) gACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTg < 1:279615/69‑1 (MQ=255) gACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTg < 1:3252674/69‑1 (MQ=255) gACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTg < 1:2244310/69‑1 (MQ=255) gACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTg < 1:2024406/69‑1 (MQ=255) gACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTg < 1:1714953/69‑1 (MQ=255) gACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTg < 1:1649372/69‑1 (MQ=255) gACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTg < 1:1342986/69‑1 (MQ=255) gACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTg < 1:1322169/69‑1 (MQ=255) gACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTg < 1:1104974/69‑1 (MQ=255) aGTAGAATGCGCAATGCGGCAACGGTGTGGAGAAGGGATaaaaaaa > 1:1549790/1‑46 (MQ=255) aGTAGAATGCGCAATGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGccc > 1:3286784/1‑68 (MQ=255) aGTAGAATGCGCAATGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGccc > 1:3330322/1‑68 (MQ=255) aGTAGAATGCGCAATGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGccc > 1:783650/1‑68 (MQ=255) aGTAGAATGCGCAATGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGccc > 1:87054/1‑68 (MQ=255) aGTAGAATGCGCAATGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGccc > 1:2684132/1‑68 (MQ=255) aGTAGAATGCGCAATGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGccc > 1:2775569/1‑68 (MQ=255) aGTAGAATGCGCAATGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGccc > 1:1674936/1‑68 (MQ=255) aGTAGAATGCGCAATGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGccc > 1:254868/1‑68 (MQ=255) aGTAGAATGCGCAATGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGccc > 1:2264034/1‑68 (MQ=255) aGTAGAATGCGCAATGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGcc > 1:1597776/1‑67 (MQ=255) aGTAGAATGCGCAATGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGcc > 1:2234603/1‑67 (MQ=255) tGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGt < 1:2395614/48‑1 (MQ=255) tGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGt < 1:694304/48‑1 (MQ=255) tGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGt < 1:644736/48‑1 (MQ=255) tGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGt < 1:874923/48‑1 (MQ=255) tGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGt < 1:115640/48‑1 (MQ=255) tGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGt < 1:2412692/48‑1 (MQ=255) tGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGt < 1:2426341/48‑1 (MQ=255) tGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGt < 1:288261/48‑1 (MQ=255) tGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGt < 1:2834635/48‑1 (MQ=255) tGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGt < 1:2939294/48‑1 (MQ=255) tGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGAtt > 1:90395/1‑68 (MQ=255) tGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGAtt > 1:1775089/1‑68 (MQ=255) tGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGAtt > 1:2120459/1‑68 (MQ=255) tGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGAtt > 1:456972/1‑68 (MQ=255) cgcAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTg > 1:2736408/1‑51 (MQ=255) cgcAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGAtt > 1:8931/1‑66 (MQ=255) cgcAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGAtt > 1:3294252/1‑66 (MQ=255) cgcAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGAt > 1:786846/1‑65 (MQ=255) aCGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCagag < 1:371018/69‑1 (MQ=255) | AACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAG > minE/1956305‑1956437 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |