| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,959,154 | G→T | 57.5% | A306E (GCG→GAG) ‡ | fbaA ← | fructose‑bisphosphate aldolase, class II |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,959,154 | 0 | G | T | 57.5% | 19.5 / 83.0 | 80 | A306E (GCG→GAG) ‡ | fbaA | fructose‑bisphosphate aldolase, class II |
| Reads supporting (aligned to +/- strand): ref base G (14/20); new base T (24/22); total (38/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.71e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTTCTTGTTCGGCTGATCTTCGCCTTTCGGGTTACCCAGCTGACCCTGCAGATAAGCTTCGTTCGCTTTGTAGTAGTTCAGAACGCCTTCCCAGGTTGCCCATTGGGTATCGGTATCGATGTTC > minE/1959090‑1959213 | tttCTTGTTCGGCTGATCTTCGCCTTTCGGGTTACCCAGCTGACCCTGCAGATAAGCTTCGTTCGCtt > 1:1144551/1‑68 (MQ=255)tttCTTGTTCGGCTGATCTTCGCCTTTCGGGTTACCCAGCTGACCCTGCAGATAAGCTTCGTTCGCtt > 1:2225171/1‑68 (MQ=255)tttCTTGTTCGGCTGATCTTCGCCTTTCGGGTTACCCAGCTGACCCTGCAGATAAGCTTCGTTCGCtt > 1:2209122/1‑68 (MQ=255)tttCTTGTTCGGCTGATCTTCGCCTTTCGGGTTACCCAGCTGACCCTGCAGATAAGCTTCGTTCGCtt > 1:2589353/1‑68 (MQ=255)tttCTTGTTCGGCTGATCTTCGCCTTTCGGGTTACCCAGCTGACCCTGCAGATAAGCTTCGTTCGCtt > 1:3364083/1‑68 (MQ=255)tttCTTGTTCGGCTGATCTTCGCCTTTCGGGTTACCCAGCTGACCCTGCAGATAAGCTTCGTTCGCtt > 1:1772682/1‑68 (MQ=255)tttCTTGTTCGGCTGATCTTCGCCTTTCGGGTTACCCAGCTGACCCTGCAGATAAGCTTCGTTCGCtt > 1:1622108/1‑68 (MQ=255)tttCTTGTTCGGCTGATCTTCGCCTTTCGGGTTACACAGCTGACCCTGCAGATAAGCTTCGTTCGCtt > 1:1439971/1‑68 (MQ=255) tCTTGTTCGGCTGATCTTCGCCTTTCGGGTTACCCAGCTGACCCTGCAGATAAGCTTCGTTCGGTTTg < 1:2959711/68‑1 (MQ=255) tCTTGTTCGGCTGATCTTCGCCTTTCGGGTTACCCAGCTGACCCTGCAGATAAGCTTCGTTCGCTTTg < 1:1816890/68‑1 (MQ=255) tCTTGTTCGGCTGATCTTCGCCTTTCGGGTTACCCAGCTGACCCTGCAGATAAGCTTCGTTCGCTTTg < 1:2396293/68‑1 (MQ=255) tCTTGTTCGGCTGATCTTCGCCTTTCGGGTTACCCAGCTGACCCTGCAGATAAGCTTCGTTCGCTTTg < 1:1846286/68‑1 (MQ=255) tCTTGTTCGGCTGATCTTCGCCTTTCGGGTTACCCAGCTGACCCTGCAGATAAGCTTCGTTCGCTTTg < 1:1687158/68‑1 (MQ=255) cTTCGCCTTTCGGGTTTCCCAGCTGACCCTGCAGATAAGCTTCGTTCGCTTTGTAGTAGTTCAGAACg < 1:276956/68‑1 (MQ=255) cTTCGCCTTTCGGGTTACCCAGCTGACCCTGCAGATAAGCTTCGTTCGCtt < 1:2374934/51‑1 (MQ=255) cTTCGCCTTTCGGGTTACCCAGCTGACCCTGCAGATAAGCTTCGTTCGCtt < 1:1169303/51‑1 (MQ=255) cTTCGCCTTTCGGGTTACCCAGCTGACCCTGCAGATAAGCTTCGTTCGCtt < 1:489486/51‑1 (MQ=255) cTTCGCCTTTCGGGTTACCCAGCTGACCCTGCAGATAAGCTTCGTTCGCtt < 1:453229/51‑1 (MQ=255) cTTCGCCTTTCGGGTTACCCAGCTGACCCTGCAGATAAGCTTCGTTCGCtt < 1:255068/51‑1 (MQ=255) cTTCGCCTTTCGGGTTACCCAGCTGACCCTGCAGATAAGCTTCGTTCGCtt < 1:780283/51‑1 (MQ=255) cTTCGCCTTTCGGGTTACCCAGCTGACCCTGCAGATAAGCTTCGTTCGCtt < 1:1898485/51‑1 (MQ=255) cTTCGCCTTTCGGGTTACCCAGCTGACCCTGCAGATAAGCTTCGTTCGCtt < 1:2967412/51‑1 (MQ=255) cTTCGCCTTTCGGGTTACCCAGCTGACCCTGCAGATAAGCTTCGTTCGCtt < 1:2957927/51‑1 (MQ=255) cTTCGCCTTTCGGGTTACCCAGCTGACCCTGCAGATAAGCTTCGTTCGCTTTgt > 1:1239236/1‑54 (MQ=255) cTTCGCCTTTCGGGTTACCCAGCTGACCCTGCAGATAAGCTTCGTTCGCTTTgt > 1:2734838/1‑54 (MQ=255) cTTCGCCTTTCGGGTTACCCAGCTGACCCTGCAGATAAGCTTCGTTCGCTTTGTAGTAGTTCAGAACg < 1:726915/68‑1 (MQ=255) cTTCGCCTTTCGGGTTACCCAGCTGACCCTGCAGATAAGCTTCGTTCGCTTTGTAGTAGTTCAGAACg < 1:380759/68‑1 (MQ=255) cTTCGCCTTTCGGGTTACCCAGCTGACCCTGCAGATAAGCTTCGTTCGCTTTGTAGTAGTTCAGAACg < 1:1626444/68‑1 (MQ=255) tGACCCTGCAGATAAGCTTCGTTCGCTTTGTAGTAGTTCAGAACg < 1:210232/45‑1 (MQ=255) gCAGGTGAGCTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCa > 1:7250/1‑55 (MQ=255) gCAGGTAAGCTTCGTTTTCTTTTTAGTAGTTCAGAACACCTTCCCAGGTTGCCCa > 1:2980771/1‑55 (MQ=255) gCAGGTAAGCTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCa > 1:973566/1‑55 (MQ=255) gCAGGTAAGCTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCa > 1:89837/1‑55 (MQ=255) gCAGGTAAGCTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCa > 1:2929410/1‑55 (MQ=255) gCAGGTAAGCTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCa > 1:2787165/1‑55 (MQ=255) gCAGGTAAGCTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCa > 1:999224/1‑55 (MQ=255) gCAGGTAAGCTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCa > 1:3434956/1‑55 (MQ=255) gCAGGTAAGCTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCa > 1:733216/1‑55 (MQ=255) gCAGGTAAGCTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCa > 1:2529852/1‑55 (MQ=255) gCAGGTAAGCTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCa > 1:982606/1‑55 (MQ=255) gCAGGTAAGCTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCa > 1:1042252/1‑55 (MQ=255) gCAGGTAAGCTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCa > 1:2278199/1‑55 (MQ=255) gCAGGTAAGCTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCa > 1:2233008/1‑55 (MQ=255) gCAGGTAAGCTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCa > 1:1313681/1‑55 (MQ=255) gCAGGTAAGCTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCa > 1:1735115/1‑55 (MQ=255) gCAGGTAAGCTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCa > 1:1771902/1‑55 (MQ=255) gCAGGTAAGCTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCa > 1:1818750/1‑55 (MQ=255) gCAGGTAAGCTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCa > 1:1838481/1‑55 (MQ=255) gCAGGTAAGCTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCa > 1:1855275/1‑55 (MQ=255) gCAGGTAAGCTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCa > 1:1905179/1‑55 (MQ=255) gCAGGTAAGCTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCa > 1:200322/1‑55 (MQ=255) gCAGGTAAGCTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCa > 1:2029302/1‑55 (MQ=255) gCAGGTAAGCTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCa > 1:2261950/1‑55 (MQ=255) cAGGTAAGCTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCAtt < 1:218172/56‑1 (MQ=255) gATAAGCTTCGTTCGCTTTGTAGTAGTTCAGAACGCCTTCCCAGGTTGCCCATTGggtatcggtatc < 1:293455/67‑1 (MQ=255) aGCTTCGTTCGCTTTGTAGTAGTTCAGAACGCCTTCCCAGGTTGCCCATTGGGTATCGGTATCGATGtt > 1:289341/1‑69 (MQ=255) aGCTTCGTTCGCTTTGTAGTAGTTCAGAACGCCTTCCCAGGTTGCCCATTGGGTATCGGTATCGATGtt > 1:1259209/1‑69 (MQ=255) aGCTTCGTTCGCTTTGTAGTAGTTCAGAACGCCTTCCCAGGTTGCCCATTGGGTATCGGTATCGATGtt > 1:1822309/1‑69 (MQ=255) aGCTTCGTTCGCTTTGTAGTAGTTCAGAACGCCTTCCCAGGTTGCCCATTGGGTATCGGTATCGATGtt > 1:1791243/1‑69 (MQ=255) cTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCATTGGGTATCGGTATCGATGTTc < 1:1230458/68‑1 (MQ=255) cTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCATTGGGTATCGGTATCGATGTTc < 1:2600841/68‑1 (MQ=255) cTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCATTGGGTATCGGTATCGATGTTc < 1:836738/68‑1 (MQ=255) cTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCATTGGGTATCGGTATCGATGTTc < 1:1397035/68‑1 (MQ=255) cTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCATTGGGTATCGGTATCGATGTTc < 1:1526647/68‑1 (MQ=255) cTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCATTGGGTATCGGTATCGATGTTc < 1:1594137/68‑1 (MQ=255) cTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCATTGGGTATCGGTATCGATGTTc < 1:1714090/68‑1 (MQ=255) cTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCATTGGGTATCGGTATCGATGTTc < 1:618753/68‑1 (MQ=255) cTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCATTGGGTATCGGTATCGATGTTc < 1:1717966/68‑1 (MQ=255) cTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCATTGGGTATCGGTATCGATGTTc < 1:1179747/68‑1 (MQ=255) cTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCATTGGGTATCGGTATCGATGTTc < 1:2445671/68‑1 (MQ=255) cTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCATTGGGTATCGGTATCGATGTTc < 1:3422099/68‑1 (MQ=255) cTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCATTGGGTATCGGTATCGATGTTc < 1:2090839/68‑1 (MQ=255) cTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCATTGGGTATCGGTATCGATGTTc < 1:2083953/68‑1 (MQ=255) cTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCATTGGGTATCGGTATCGATGTTc < 1:1845109/68‑1 (MQ=255) cTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCATTGGGTATCGGTATCGATGTTc < 1:2584257/68‑1 (MQ=255) cTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCATTGGGTATCGGTATCGATGTTc < 1:18601/68‑1 (MQ=255) cTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCATTGGGTATCGGTATCGATGTTc < 1:29207/68‑1 (MQ=255) cTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCATTGGGTATCGGTATCGATGTTc < 1:2792009/68‑1 (MQ=255) cTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTCCCAGGTTGCCCATTGGGTATCGGTATCGATGTTc < 1:1893435/68‑1 (MQ=255) cTTCGTTTTCTTTGTAGTAGTTCAGAACACCTTACCAGGTTGCCCATTGGGTATCGGTATCGATGTTc < 1:3290422/68‑1 (MQ=255) | TTTCTTGTTCGGCTGATCTTCGCCTTTCGGGTTACCCAGCTGACCCTGCAGATAAGCTTCGTTCGCTTTGTAGTAGTTCAGAACGCCTTCCCAGGTTGCCCATTGGGTATCGGTATCGATGTTC > minE/1959090‑1959213 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |