| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 21,895 | T→C | 24.7% | N609N (AAT→AAC) | ileS → | isoleucyl‑tRNA synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 21,895 | 0 | T | C | 24.7% | 136.6 / 41.1 | 113 | N609N (AAT→AAC) | ileS | isoleucyl‑tRNA synthetase |
| Reads supporting (aligned to +/- strand): ref base T (24/61); new base C (11/17); total (35/78) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.46e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 118 total aligned reads displayed.
GTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAA > minE/21828‑21962 | gTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAAt < 1:2223315/68‑1 (MQ=255)gTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAAt < 1:811233/68‑1 (MQ=255)gTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAAt < 1:593613/68‑1 (MQ=255)gTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAAt < 1:885783/68‑1 (MQ=255) gTACTGACCCACGCCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAATACCGt < 1:2336118/68‑1 (MQ=255) aCTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAATACCGttt > 1:2226355/1‑68 (MQ=255) aCTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAATACCGTTTc > 1:2609097/1‑69 (MQ=255) cGGCTTTACCGTGGATTGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGa < 1:2329408/69‑1 (MQ=255) cGGCTTTACCGTGGATGGTGAGGGCCGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGa < 1:3268413/69‑1 (MQ=255) cGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGa < 1:646438/69‑1 (MQ=255) cGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGa < 1:2205219/69‑1 (MQ=255) cGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGa < 1:2322897/69‑1 (MQ=255) cGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGa < 1:2592417/69‑1 (MQ=255) cGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGa < 1:1515363/69‑1 (MQ=255) cGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGa < 1:3300091/69‑1 (MQ=255) cGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGa < 1:1265071/69‑1 (MQ=255) ggCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGa < 1:50195/68‑1 (MQ=255) tttACCGTGGATGGTTAGGGCCGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGAtgtg < 1:398903/69‑1 (MQ=255) tttACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGAtgtg < 1:2070071/69‑1 (MQ=255) tttACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGAtgtg < 1:1022603/69‑1 (MQ=255) tttACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGAtgtg < 1:3357151/69‑1 (MQ=255) tttACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGAtgtg < 1:2852286/69‑1 (MQ=255) tttACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGAtgtg < 1:165338/69‑1 (MQ=255) tttACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGAtgtg < 1:1270440/69‑1 (MQ=255) tttACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGAtgtg < 1:390798/69‑1 (MQ=255) gTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTGATGaa > 1:925657/1‑68 (MQ=255) gTGGATGGTCAGGGC‑‑CAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTGATGAACaa > 1:3156640/1‑69 (MQ=255) ggATGGTCAGGTCCGCTAGATGTCTAAATCCATCGGCAATACCTTTTCGCCGCATGa < 1:2561992/57‑1 (MQ=255) gATGGTCAGGGCCGCAAGATGTCTAAATCCTTCGGCAATACCGTTTCCCCGCAGGAggtg > 1:3327924/1‑60 (MQ=255) gATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTGATGAACaaa > 1:1728378/1‑69 (MQ=255) gATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTGATGAACaaa > 1:210114/1‑69 (MQ=255) ggTCAGGGCCGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTGATGAACAAACTg < 1:3208254/69‑1 (MQ=255) ggTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTcg > 1:595417/1‑45 (MQ=255) ggTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTg > 1:785747/1‑69 (MQ=255) ggTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTg > 1:2801611/1‑69 (MQ=255) ggTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTg > 1:2706172/1‑69 (MQ=255) ggTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTg > 1:1031934/1‑69 (MQ=255) ggTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTg > 1:952981/1‑69 (MQ=255) ggTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTg > 1:1787049/1‑69 (MQ=255) gTCAGGGCCGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTGATGAACAAACTgg > 1:753773/1‑69 (MQ=255) gTCAGGGCCGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTGATGAACAAACTgg > 1:1381945/1‑69 (MQ=255) gTCAGGGCCGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTGATGAACAAACTg > 1:2204608/1‑68 (MQ=255) gCCGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGa < 1:1466083/46‑1 (MQ=255) gCCGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGAtgtg < 1:154793/50‑1 (MQ=255) gCCGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGcgcg > 1:1124839/1‑68 (MQ=255) gCCGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGcgcg > 1:1207276/1‑68 (MQ=255) gCCGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGcgcg > 1:2272489/1‑68 (MQ=255) gCCGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGcgcg > 1:2998975/1‑68 (MQ=255) gCCGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGcgcg > 1:2035141/1‑68 (MQ=255) ccGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGa < 1:2117816/69‑1 (MQ=255) ccGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGa < 1:377321/69‑1 (MQ=255) ccGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGa < 1:2750868/69‑1 (MQ=255) ccGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGa < 1:1561059/69‑1 (MQ=255) ccGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGa < 1:412877/69‑1 (MQ=255) ccGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGa < 1:831151/69‑1 (MQ=255) ccGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGa < 1:802247/69‑1 (MQ=255) ccGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGa < 1:1806111/69‑1 (MQ=255) ccGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGa < 1:2267924/69‑1 (MQ=255) ccGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGa < 1:1886267/69‑1 (MQ=255) ccGCAAGATGTCTAAATCCATCGGCAATACCGTTTCCCCGCAGGATGTGATGAACAAACTGGGCGCGGa < 1:808492/69‑1 (MQ=255) gTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCg < 1:158848/69‑1 (MQ=255) gTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCg < 1:1059762/69‑1 (MQ=255) gTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCg < 1:1207163/69‑1 (MQ=255) gTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCg < 1:926907/69‑1 (MQ=255) gTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCg < 1:128794/69‑1 (MQ=255) gTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCg < 1:2389665/69‑1 (MQ=255) gTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCg < 1:831850/69‑1 (MQ=255) gTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCg < 1:1738554/69‑1 (MQ=255) tCTAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCg < 1:2027573/68‑1 (MQ=255) cTAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCg < 1:991646/67‑1 (MQ=255) tAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGt > 1:3325650/1‑67 (MQ=255) tAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCt > 1:1121872/1‑69 (MQ=255) tAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCt > 1:1457222/1‑69 (MQ=255) tAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCt > 1:493688/1‑69 (MQ=255) aaTCCATCGGCAATACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCtgt > 1:749987/1‑69 (MQ=255) aaTCCATCGGCAATACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCtgt > 1:2472645/1‑69 (MQ=255) tCCATCGGCAATACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCtgtg < 1:2480674/68‑1 (MQ=255) tCCATCGGCAATACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCtgtg < 1:2113688/68‑1 (MQ=255) tCCATCGGCAATACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCtgtg < 1:2134183/68‑1 (MQ=255) tCCATCGGCAATACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCtgtg < 1:8689/68‑1 (MQ=255) tCCATCGGCAATACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCtg < 1:2580234/66‑1 (MQ=255) tCCATCGGCAATACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCtg < 1:70833/66‑1 (MQ=255) tCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCtgt < 1:1079369/67‑1 (MQ=255) tCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCtgt < 1:1669038/67‑1 (MQ=255) tCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCtgt < 1:1991530/67‑1 (MQ=255) tCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCtgt < 1:1309733/67‑1 (MQ=255) tCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCtgt < 1:1241383/67‑1 (MQ=255) tCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCtgt < 1:963753/67‑1 (MQ=255) tCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCtgt < 1:2020185/67‑1 (MQ=255) tCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCtgt < 1:2161562/67‑1 (MQ=255) tCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCtgt < 1:2290694/67‑1 (MQ=255) tCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCtgt < 1:510140/67‑1 (MQ=255) tCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCtgt < 1:2772584/67‑1 (MQ=255) tCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCtgt < 1:3391240/67‑1 (MQ=255) ccATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGGGTCtgt < 1:4164/66‑1 (MQ=255) cATCGGCAATACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTgtgggt > 1:3081214/1‑69 (MQ=255) cGGCAATACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGc < 1:2960576/69‑1 (MQ=255) ggCAATACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGatat < 1:2022761/50‑1 (MQ=255) cAATACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCAt < 1:1378564/68‑1 (MQ=255) aTACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCaa < 1:2682137/69‑1 (MQ=255) | GTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAA > minE/21828‑21962 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |