| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 148,925 | G→T | 58.9% | G694V (GGA→GTA) | hrpB → | predicted ATP‑dependent helicase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 148,925 | 0 | G | T | 58.9% | 16.9 / 54.7 | 56 | G694V (GGA→GTA) | hrpB | predicted ATP‑dependent helicase |
| Reads supporting (aligned to +/- strand): ref base G (13/10); new base T (26/7); total (39/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.67e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATC > minE/148861‑148989 | cATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGa > 1:2904961/1‑66 (MQ=255) ggCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTg < 1:2707373/69‑1 (MQ=255) aaTCACTCGATATTTATCAGGCACTGCGTGGATTACTTGATTGGGTAATGCAGCAAc > 1:673008/1‑57 (MQ=255) aaTCACTCGATATTTATCAGGCACTGCGTGGATTACTTGATTGGGTAATGCAGCAAc > 1:2897989/1‑57 (MQ=255) aaTCACTCGATATTTATCAGGCACTGCGTGGATTACTTGATTGGGTAATGCAGCAAc > 1:1466616/1‑57 (MQ=255) aaTCACTCGATATTTATCAGGCACTGCGTGGATTACTTGATTGGGTAATGCAGCAACg > 1:211965/1‑58 (MQ=255) aaTCACTCGATATTTATCAGGCACTGCGTGGATTACTTGATTGGGTAATGCAGCAACg > 1:1099266/1‑58 (MQ=255) aaTCACTCGATATTTATCAGGCACTGCGTGGATTACTTGATTGGGTAATGCAGCAACg > 1:2239662/1‑58 (MQ=255) aaTCACTCGATATTTATCAGGCACTGCGTGGATTACTTGATTGGGTAATGCAGCAACg > 1:2265562/1‑58 (MQ=255) aaTCACTCGATATTTATCAGGCACTGCGTGGATTACTTGATTGGGTAATGCAGCAACg > 1:2785088/1‑58 (MQ=255) aaTCACTCGATATTTATCAGGCACTGCGTGGATTACTTGATTGGGTAATGCAGCAACg > 1:2796979/1‑58 (MQ=255) aaTCACTCGATATTTATCAGGCACTGCGTGGATTACTTGATTGGGTAATGCAGCAACg > 1:2883623/1‑58 (MQ=255) aaTCACTCGATATTTATCAGGCACTGCGTGGATTACTTGATTGGGTAATGCAGCAACg > 1:3190253/1‑58 (MQ=255) aaTCACTCGATATTTATCAGGCACTGCGTGGATTACTTGATTGGGTAATGCAGCAACg > 1:323742/1‑58 (MQ=255) aaTCACTCGATATTTATCAGGCACTGCGTGGATTACTTGATTGGGTAATGCAGCAACg > 1:3427916/1‑58 (MQ=255) aaTCACTCGATATTTATCAGGCACTGCGTGGATTACTTGATTGGGTAATGCAGCAACg > 1:447417/1‑58 (MQ=255) aaTCACTCGATATTTATCAGGCACTGCGTGGATTACTTGATTGGGTAATGCAGCAACg > 1:467820/1‑58 (MQ=255) aaTCACTCGATATTTATCAGGCACTGCGTGGATTACTTGATTGGGTAATGCAGCAACg > 1:907407/1‑58 (MQ=255) aaTCACTCGATATTTATCAGGCACTGCGTGGATTACTTGATTGGGTAATGCAGCAACg > 1:1821939/1‑58 (MQ=255) aaTCACTCGATATTTATCAGGCACTGCGTGGATTACTTGATTGGGTAATGCAGCAACg > 1:1065860/1‑58 (MQ=255) aaTCACTCGATATTTATCAGGCACTGCGTGGATTACTTGATTGGGTAATGCAGCAACg > 1:1753849/1‑58 (MQ=255) aaTCACTCGATATTTATCAGGCACTGCGTGGATTACTTGATTGGGTAATGCAGCAACg > 1:1110830/1‑58 (MQ=255) aaTCACTCGATATTTATCAGGCACTGCGTGGATTACTTGATTGGGTAATGCAGCAACg > 1:1182674/1‑58 (MQ=255) aaTCACTCGATATTTATCAGGCACTGCGTGGATTACTTGATTGGGTAATGCAGCAACg > 1:1576817/1‑58 (MQ=255) aaTCACTCGATATTTATCAGGCACTGCGTGGATTACTTGATTGGGTAATGCAGCAACg > 1:134512/1‑58 (MQ=255) aaTCACTCGATATTTATCAGGCACTGCGTGGATTACTTGATTGGGTAATGCAGCAACg > 1:1557250/1‑58 (MQ=255) aaTCACTCGATATTTATCAGGCACTGCGTGGATTACTTGATTGGGTAATGCAGCAACg > 1:1452912/1‑58 (MQ=255) aaTCACTCGATATTTATCAGGCACTGCGTGGATTACTTGATTGGGTAATACAGCAACg > 1:2790594/1‑58 (MQ=255) tCGATATTTATCAGGCACTGCGTGGATTACTTGATTGGGTAATGCAGCAACGTCTGGATAGTGAATTGc < 1:992673/69‑1 (MQ=255) tCGATATTTATCAGGCACTGCGTGGATTACTTGATTGGGTAATGCAGCAACGTCTGGATAGTGAATTGc < 1:1871733/69‑1 (MQ=255) tCGATATTTATCAGGCACTGCGTGGATTACTTGATTGGGTAATGCAGCAACGTCTGGATAGTGAATTGc < 1:444617/69‑1 (MQ=255) tCGATATTTATCAGGCACTGCGTGGATTACTTGATTGGGTAATGCAGCAACGTCTGGATAGTGAATTGc < 1:2932262/69‑1 (MQ=255) tCGATATTTATCAGGCACTGCGTGGATTACTTGATTGGGTAATGCAGCAACGTCTGGATAGTGAATTGc < 1:141690/69‑1 (MQ=255) tCGATATTTATCAGGCACTGCGTGGATTACTTGATTGGGTAATGCAGCAACGTCTGGATAGTGAATTGc < 1:1535134/69‑1 (MQ=255) tCGATATTTATCAGGCACTGCGTGGATTACTTGATTGGGTAATGCAGCAACGTCTGGATAGTGAATTGc < 1:2709310/69‑1 (MQ=255) aTTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTg > 1:2303631/1‑58 (MQ=255) tATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCAt > 1:3197354/1‑69 (MQ=255) tATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCAt > 1:2592408/1‑69 (MQ=255) tATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCAt > 1:1178586/1‑69 (MQ=255) tATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTACCTGCGCAt > 1:1169119/1‑69 (MQ=255) tcTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTg < 1:76544/46‑1 (MQ=255) aCGCGGATTACTTGGTTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTgcg > 1:3075882/1‑55 (MQ=255) aCGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATa > 1:1453328/1‑41 (MQ=255) aCGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTgcg > 1:79873/1‑55 (MQ=255) aCGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTgcg > 1:92673/1‑55 (MQ=255) aCGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTgcg < 1:322351/55‑1 (MQ=255) aCGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTgcg > 1:2720025/1‑55 (MQ=255) aCGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTgcg > 1:1122985/1‑55 (MQ=255) aCGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTgcg > 1:736474/1‑55 (MQ=255) aCGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGTGcc < 1:2859149/69‑1 (MQ=255) aCGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGTGcc < 1:3396871/69‑1 (MQ=255) aCGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGTGcc < 1:3276502/69‑1 (MQ=255) cgcgGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTgcg < 1:845935/54‑1 (MQ=255) ttGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCgg < 1:612233/67‑1 (MQ=255) ttGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCgg < 1:2695823/67‑1 (MQ=255) ttGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCgg < 1:2089727/67‑1 (MQ=255) ggggAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATc > 1:583318/1‑68 (MQ=255) ggggAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATc > 1:1809096/1‑68 (MQ=255) | CATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATC > minE/148861‑148989 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |