Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | minE | 1,959,701 | A→G | 58.3% | L124L (TTG→CTG) | fbaA ← | fructose‑bisphosphate aldolase, class II |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | minE | 1,959,701 | 0 | A | G | 58.3% | 24.0 / 47.1 | 67 | L124L (TTG→CTG) | fbaA | fructose‑bisphosphate aldolase, class II |
Reads supporting (aligned to +/- strand): ref base A (4/24); new base G (21/18); total (25/42) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.01e-03 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.43e-01 |
CAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGTTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGG > minE/1959633‑1959761 | cAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGTTTTTCACCCGCGTCCaa < 1:1074329/69‑1 (MQ=255) cAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGTTTTTCACCCGCGTCCaa < 1:346601/69‑1 (MQ=255) gTCAATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCAGCGAAGTGTTTTTCACCCGCGTCcagcag > 1:923993/1‑69 (MQ=255) gTCAATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCAGCGAAGTGTTTTTCACCCGCGTCcagcag > 1:865327/1‑69 (MQ=255) gTCAATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCAGCGAAGTGTTTTTCACCCGCGTCcagcag > 1:751493/1‑69 (MQ=255) gTCAATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCAGCGAAGTGTTTTTCACCCGCGTCcagcag > 1:67051/1‑69 (MQ=255) gTCAATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCAGCGAAGTGTTTTTCACCCGCGTCcagcag > 1:3421315/1‑69 (MQ=255) gTCAATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCAGCGAAGTGTTTTTCACCCGCGTCcagcag > 1:3330456/1‑69 (MQ=255) gTCAATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCAGCGAAGTGTTTTTCACCCGCGTCcagcag > 1:323625/1‑69 (MQ=255) gTCAATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCAGCGAAGTGTTTTTCACCCGCGTCcagcag > 1:2884708/1‑69 (MQ=255) gTCAATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCAGCGAAGTGTTTTTCACCCGCGTCcagcag > 1:2200534/1‑69 (MQ=255) gTCAATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCAGCGAAGTGTTTTTCACCCGCGTCcagcag > 1:2151425/1‑69 (MQ=255) gTCAATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCAGCGAAGTGTTTTTCACCCGCGTCcagcag > 1:2042699/1‑69 (MQ=255) gTCAATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCAGCGAAGTGTTTTTCACCCGCGTCcagcag > 1:1957190/1‑69 (MQ=255) gTCAATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCAGCGAAGTGTTTTTCACCCGCGTCcagcag > 1:1710557/1‑69 (MQ=255) gTCAATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCAGCGAAGTGTTTTTCACCCGCGTCcagcag > 1:1685713/1‑69 (MQ=255) gTCAATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCAGCGAAGTGTTTTTCACCCGCGTCcagcag > 1:157061/1‑69 (MQ=255) gTCAATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCAGCGAAGTGTTTTTCACCCGCGTCcagcag > 1:148948/1‑69 (MQ=255) gTCAATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCAGCGAAGTGTTTTTCACCCGCGTCcagcag > 1:1110419/1‑69 (MQ=255) aGCGGCTTACCGGTAGCTGCGAAGTGTTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGcagcag > 1:2014005/1‑68 (MQ=255) aGCGGCTTACCGGTAGCTGCGAAGTGTTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGcagcag > 1:213137/1‑68 (MQ=255) aGCGGCTTACCGGTAGCTGCGAAGTGTTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGt < 1:3166381/69‑1 (MQ=255) aGCGGCTTACCGGTAGCTGCGAAGTGTTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGt > 1:3210599/1‑69 (MQ=255) aGCGGCTTACCGGTAGCTGCGAAGTGTTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGt < 1:1218713/69‑1 (MQ=255) aGCGGCTTACCGGTAGCTGCGAAGTGTTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGt < 1:554378/69‑1 (MQ=255) aGCGGCTTACCGGTAGCTGCGAAGTGTTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGt < 1:221088/69‑1 (MQ=255) aGCGGCTTACCGGTAGCTGCGAAGTGTTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGt < 1:1500760/69‑1 (MQ=255) aGCGGCTTACCGGTAGCTGCGAAGTGTTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGt < 1:1948541/69‑1 (MQ=255) gTAGCAGCGAAGTGTTTTTCACCCGCGTCCAGCAGAcc > 1:545768/1‑38 (MQ=38) gTAGCAGCGAAGTGTTTTTCACCCGCGTCCAGCAGACCGTCGATc > 1:275398/1‑45 (MQ=255) gTAGCAGCGAAGTGTTTTTCACCCGCGTCCAGCAGACCGTCGATCCACGGCAGCAGTTTCTTCgcgc > 1:661879/1‑67 (MQ=255) gTAGCAGCGAAGTGTTTTTCACCCGCGTCCAGCAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAg > 1:293131/1‑69 (MQ=255) gTAGCAGCGAAGTGTTTTTCACCCGCGTCCAGCAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAg > 1:2962035/1‑69 (MQ=255) gTAGCAGCGAAGTGTTTTTCACCCGCGTCCAGCAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAg > 1:1461455/1‑69 (MQ=255) gTAGCAGCGAAGTGTTTTTCACCCGCGTCCAGCAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAg > 1:2971926/1‑69 (MQ=255) gTAGCAGCGAAGTGTTTTTCACCCGCGTCCAGCAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAg > 1:316625/1‑69 (MQ=255) gTAGCAGCGAAGTGTTTTTCACCCGCGTCCAGCAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAg > 1:2602726/1‑69 (MQ=255) gTAGCAGCGAAGTGTTTTTCACCCGCGTCCAGCAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAg > 1:1439370/1‑69 (MQ=255) gTAGCAGCGAAGTGTTTTTCACCCGCGTCCAGCAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAg > 1:2665900/1‑69 (MQ=255) gTAGCAGCGAAGTGTTTTTCACCCGCGTCCAGCAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAg > 1:3306210/1‑69 (MQ=255) gTAGCAGCGAAGTGTTTTTCACCCGCGTCCAGCAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAg > 1:3312311/1‑69 (MQ=255) gTAGCAGCGAAGTGTTTTTCACCCGCGTCCAGCAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAg > 1:2553749/1‑69 (MQ=255) gTAGCAGCGAAGTGTTTTTCACCCGCGTCCAGCAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAg > 1:792299/1‑69 (MQ=255) gTAGCAGCGAAGTGTTTTTCACCCGCGTCCAGCAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAg > 1:818848/1‑69 (MQ=255) gTAGCAGCGAAGTGTTTTTCACCCGCGTCCAGCAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAg > 1:874143/1‑69 (MQ=255) gTAGCAGCGAAGTGTTTTTCACCCGCGTCCAGCAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAg > 1:394451/1‑69 (MQ=255) gTAGCAGCGAAGTGTTTTTCACCCGCGTCCAGCAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAg > 1:1547576/1‑69 (MQ=255) gTAGCAGCGAAGTGTTTTTCACCCGCGTCCAGCAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAg > 1:1346895/1‑69 (MQ=255) gTAGCAGCGAAGTGTTTTTCACCCGCGTCCAGCAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAg > 1:367294/1‑69 (MQ=255) aGCTGCGAAGTGTTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTg < 1:68388/69‑1 (MQ=255) aGCTGCGAAGTGTTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTg < 1:542291/69‑1 (MQ=255) gCTGCGAAGTGTTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTg < 1:2724682/68‑1 (MQ=255) tGCGAAGTGTTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTc < 1:1704185/69‑1 (MQ=255) tGCGAAGTGTTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTc < 1:1427371/69‑1 (MQ=255) tGCGAAGTGTTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTc < 1:518294/69‑1 (MQ=255) tGCGAAGTGTTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTc > 1:3170259/1‑69 (MQ=255) tGCGAAGTGTTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTc < 1:2977282/69‑1 (MQ=255) tGCGAAGTGTTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTc < 1:2129388/69‑1 (MQ=255) aaGTGTTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAg < 1:2608092/67‑1 (MQ=255) aaGTGTTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAg < 1:2088061/67‑1 (MQ=255) tttCACCCGCGTCCAGCAGACCGTCGATCCACGGcaggag < 1:1266574/40‑1 (MQ=38) tttCACCCGCGTCCAGCAGACCGTCGATCCACGGcagcag < 1:637247/40‑1 (MQ=255) tttCACCCGCGTCCAGCAGACCGTCGATCCACGGcagcag < 1:742353/40‑1 (MQ=255) tttCACCCGCGTCCAGCAGACCGTCGATCCACGGcagcag < 1:499662/40‑1 (MQ=255) tttCACCCGCGTCCAGCAGACCGTCGATCCACGGcagcag < 1:914374/40‑1 (MQ=255) tttCACCCGCGTCCAGCAGACCGTCGATCCACGGcagcag < 1:1009329/40‑1 (MQ=255) tttCACCCGCGTCCAGCAGACCGTCGATCCACGGcagcag < 1:1626979/40‑1 (MQ=255) tttCACCCGCGTCCAGCAGACCGTCGATCCACGGcagcag < 1:3389813/40‑1 (MQ=255) tttCACCCGCGTCCAGCAGACCGTCGATCCACGGcagcag < 1:3209256/40‑1 (MQ=255) tttCACCCGCGTCCAGCAGACCGTCGATCCACGGcagcag < 1:3138557/40‑1 (MQ=255) tttCACCCGCGTCCAGCAGACCGTCGATCCACGGcagcag < 1:310459/40‑1 (MQ=255) tttCACCCGCGTCCAGCAGACCGTCGATCCACGGcagcag < 1:2860359/40‑1 (MQ=255) tttCACCCGCGTCCAGCAGACCGTCGATCCACGGcagcag < 1:2700942/40‑1 (MQ=255) tttCACCCGCGTCCAGCAGACCGTCGATCCACGGcagcag < 1:2182773/40‑1 (MQ=255) tttCACCCGCGTCCAGCAGACCGTCGATCCACGGcagcag < 1:2657840/40‑1 (MQ=255) tttCACCCGCGTCCAGCAGACCGTCGATCCACGGcagcag < 1:2521361/40‑1 (MQ=255) tttCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGa < 1:1419358/69‑1 (MQ=255) tttCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGa < 1:2113367/69‑1 (MQ=255) tttCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGa < 1:844849/69‑1 (MQ=255) tttCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGa < 1:348889/69‑1 (MQ=255) ttCACCCGCGTCCAGCAGACCGTCGATCCACGGcagcag < 1:782191/39‑1 (MQ=255) ttCACCCGCGTCCAGCAGACCGTCGATCCACGGcagcag < 1:2168416/39‑1 (MQ=255) ttCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGa < 1:1806081/68‑1 (MQ=255) cgcgTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCgg < 1:2499682/69‑1 (MQ=255) cgcgTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCgg < 1:2662456/69‑1 (MQ=255) cgcgTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCgg < 1:2764623/69‑1 (MQ=255) aCAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTg < 1:2761387/40‑1 (MQ=255) | CAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGTTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGG > minE/1959633‑1959761 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |