| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,963,872 | C→G | 49.1% | V50V (GTG→GTC) | yggD ← | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,963,872 | 0 | C | G | 49.1% | ‑4.6 / 131.5 | 104 | V50V (GTG→GTC) | yggD | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base C (28/25); new base G (30/21); total (58/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.60e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.50e-01 | |||||||||||
Only 100 of 126 total aligned reads displayed.
CAGTGCAACATCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATCCCCTGGAGCAAAATGG > minE/1963809‑1963939 | cAGTGCAACATCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGAt > 1:1018968/1‑69 (MQ=255)cAGTGCAACATCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGAt > 1:3181262/1‑69 (MQ=255)cAGTGCAACATCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGAt > 1:1165025/1‑69 (MQ=255) aaCATCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCGACGATCTCTTc < 1:2671052/69‑1 (MQ=255) aaCATCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCGACGATCTCTTc < 1:1054551/69‑1 (MQ=255) aaCATCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCGACGATCTCTTc < 1:486047/69‑1 (MQ=255) aaCATCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCGACGATCTCTTc < 1:609038/69‑1 (MQ=255) aaCATCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCGACGATCTCTTc < 1:738752/69‑1 (MQ=255) aaCATCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCGACGATCTCTTc < 1:2577241/69‑1 (MQ=255) aaCATCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCGACGATCTCTTc < 1:1228146/69‑1 (MQ=255) aaCATCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCGACGATCTCTTc < 1:740531/69‑1 (MQ=255) aCATCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCGACGATCTCTTc < 1:2403085/68‑1 (MQ=255) aCATCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCGACGATCTCTTc < 1:1505912/68‑1 (MQ=255) aCATCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCa > 1:2172412/1‑69 (MQ=255) tCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATctct > 1:250090/1‑63 (MQ=255) tCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATc > 1:1432509/1‑68 (MQ=255) tCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATc > 1:315318/1‑68 (MQ=255) tCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATc > 1:852729/1‑68 (MQ=255) tCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATc > 1:1869197/1‑68 (MQ=255) atCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCGACGATCTCTTCATCGtt > 1:380746/1‑66 (MQ=255) atCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCGACGATCTCTTCATCGTTAt > 1:2294240/1‑68 (MQ=255) atCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCGACGATCTCTTCATCGTTATc > 1:1984226/1‑69 (MQ=255) atCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCGACGATCTCTTCATCGTTATc > 1:1925897/1‑69 (MQ=255) atCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCGACGATCTCTTCATCGTTATc > 1:1825878/1‑69 (MQ=255) atCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCGACGATCTCTTCATCGTTATc > 1:2003473/1‑69 (MQ=255) atCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCGACGATCTCTTCATCGTTATc > 1:1925204/1‑69 (MQ=255) atCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCGACGATCTCTTCATCGTTATc > 1:3391765/1‑69 (MQ=255) atCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCGACGATCTCTTCATCGTTATc > 1:1594091/1‑69 (MQ=255) atCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCGACGATCTCTTCATCGTTATc > 1:1529214/1‑69 (MQ=255) atCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCGACGATCTCTTCATCGTTATc > 1:2110837/1‑69 (MQ=255) atCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCGACGATCTCTTCATCGTTATc > 1:1463708/1‑69 (MQ=255) atCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCGACGATCTCTTCATCGTTATc > 1:2544214/1‑69 (MQ=255) atCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCGACGATCTCTTCATCGTTATc > 1:894171/1‑69 (MQ=255) atCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCGACGATCTCTACATCGTTATc > 1:1099605/1‑69 (MQ=255) aacGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCGACGATCTCTTCATCGTTATc > 1:628356/3‑69 (MQ=255) tCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATc > 1:2059762/1‑68 (MQ=255) tCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATc > 1:1009262/1‑68 (MQ=255) tCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATc > 1:765565/1‑68 (MQ=255) tCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATc > 1:1167972/1‑68 (MQ=255) tCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATc > 1:1204747/1‑68 (MQ=255) tCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATc > 1:149616/1‑68 (MQ=255) cAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCGACGATCTCTTCATCGTTATCGACaa < 1:1336983/69‑1 (MQ=255) cAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCGACGATCTCTTCATCGTTATCGACaa < 1:2460153/69‑1 (MQ=255) cAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCGACGATCTCTTCATCGTTATCGACaa < 1:1027184/69‑1 (MQ=255) cAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCGACGATCTCTTCATCGTTATCGACaa < 1:2318549/69‑1 (MQ=255) cAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCGACGATCTCTTCATCGTTATCGACaa < 1:2909345/69‑1 (MQ=255) cAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCGACGATCTCTTCATCGTTATCGACaa < 1:429789/69‑1 (MQ=255) cAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCGACGATCTCTTCATCGTTATCGACaa < 1:420700/69‑1 (MQ=255) aGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCGACGATCTCTTCATCGTTATCGACaa < 1:3357047/67‑1 (MQ=255) ggCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCg < 1:2305015/69‑1 (MQ=255) ggCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCg < 1:3055037/69‑1 (MQ=255) gCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCg < 1:2757521/68‑1 (MQ=255) cGCTTTGGGCGAGTAACGGCTTCACTGCATATTcc > 1:3125079/1‑35 (MQ=255) cGCTTTGGGCGAGTAACGGCTTAACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGAc > 1:924933/1‑68 (MQ=255) tttGGGCGAGTAACGGCTTCACTGCATATTCGACGATCTCTTCATCGTTATCGACAAACAGCGACgg > 1:285054/1‑67 (MQ=255) tttGGGCGAGTAACGGCTTCACTGCATATTCGACGATCTCTTCATCGTTATCGACAAACAGCGACGGc > 1:2414433/1‑68 (MQ=255) tttGGGCGAGTAACGGCTTCACTGCATATTCGACGATCTCTTCATCGTTATCGACAAACAGCGACGGc > 1:16365/1‑68 (MQ=255) tttGGGCGAGTAACGGCTTCACTGCATATTCGACGATCTCTTCATCGTTATCGACAAACAGCGACGGc > 1:254648/1‑68 (MQ=255) tttGGGCGAGTAACGGCTTCACTGCATATTCGACGATCTCTTCATCGTTATCGACAAACAGCGACGGc > 1:2581882/1‑68 (MQ=255) ggCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGa < 1:657277/67‑1 (MQ=255) aGTAACGGCTTCCCTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTgg < 1:282261/69‑1 (MQ=255) aGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTgg < 1:1642687/69‑1 (MQ=255) aGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTgg < 1:1480773/69‑1 (MQ=255) aGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTgg < 1:176875/69‑1 (MQ=255) aGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTgg < 1:178894/69‑1 (MQ=255) gTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTgg < 1:2756311/68‑1 (MQ=255) aaCGGCTTCTCTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGt < 1:488084/69‑1 (MQ=255) aaCGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGt < 1:135289/69‑1 (MQ=255) aaCGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGt < 1:2966662/69‑1 (MQ=255) aaCGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGt < 1:1137552/69‑1 (MQ=255) aaCGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGt < 1:2110091/69‑1 (MQ=255) aaCGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGt < 1:2582124/69‑1 (MQ=255) aaCGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGt < 1:424020/69‑1 (MQ=255) aCGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTa > 1:3083822/1‑69 (MQ=255) aCGGCTTCACCGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGt < 1:510283/68‑1 (MQ=255) tCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGt < 1:1164488/62‑1 (MQ=255) cATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATCCCCTGGAGc > 1:2357770/1‑68 (MQ=255) atTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGt < 1:1534333/53‑1 (MQ=255) ttCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATCCCCTGGAGCAAAAt > 1:879314/1‑69 (MQ=255) ttCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATCCCCTGGAGCAAAAt > 1:537536/1‑69 (MQ=255) ttCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATCCCCTGGAGCAAAAt > 1:1578701/1‑69 (MQ=255) ttCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGAGTATCCCCTGGAGCAAAAt > 1:2939898/1‑69 (MQ=255) ccACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGa > 1:1007188/1‑41 (MQ=255) ccACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGa > 1:2470025/1‑41 (MQ=255) ccACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGa > 1:1514930/1‑41 (MQ=255) ccACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGa > 1:1398769/1‑41 (MQ=255) ccACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGa > 1:1138283/1‑41 (MQ=255) ccACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGa > 1:153898/1‑41 (MQ=255) ccACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGa > 1:3262163/1‑41 (MQ=255) ccACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGa > 1:1695429/1‑41 (MQ=255) ccACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGa > 1:1884985/1‑41 (MQ=255) ccACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGa > 1:2063359/1‑41 (MQ=255) ccACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGa > 1:2718731/1‑41 (MQ=255) ccACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATCCCCTGGAGCAAAATgg < 1:407122/69‑1 (MQ=255) ccACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATCCCCTGGAGCAAAATgg < 1:579164/69‑1 (MQ=255) ccACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATCCCCTGGAGCAAAATgg < 1:1668426/69‑1 (MQ=255) ccACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATCCCCTGGAGCAAAATgg < 1:1774353/69‑1 (MQ=255) ccACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATCCCCTGGAGCAAAATgg < 1:2832704/69‑1 (MQ=255) ccACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATCCCCTGGAGCAAAATgg < 1:2744410/69‑1 (MQ=255) ccACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATCCCCTGGAGCAAAATgg < 1:2269564/69‑1 (MQ=255) | CAGTGCAACATCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATCCCCTGGAGCAAAATGG > minE/1963809‑1963939 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |