| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,966,806 | G→A | 42.0% | R608R (CGC→CGT) | tktA ← | transketolase 1, thiamin‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,966,806 | 0 | G | A | 42.0% | 40.9 / 77.8 | 107 | R608R (CGC→CGT) | tktA | transketolase 1, thiamin‑binding |
| Reads supporting (aligned to +/- strand): ref base G (46/16); new base A (22/23); total (68/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.73e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 107 total aligned reads displayed.
CGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCA > minE/1966742‑1966863 | cgaTAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCGCTTCTACAGCAACGCGTg < 1:2050901/69‑1 (MQ=255)cgaTAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCGCTTCTACAGCAACGCGTg < 1:2405162/69‑1 (MQ=255)cgaTAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCGCTTCTACAGCAACGCGTg < 1:1746953/69‑1 (MQ=255)cgaTAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCGCTTCTACAGCAACGCGTg < 1:1750765/69‑1 (MQ=255) ttCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCt < 1:3360125/69‑1 (MQ=255) aCTTGTACCAGTAGTCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACgg < 1:2683651/69‑1 (MQ=255) cTTGTACCAGTAGTCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACgg > 1:377830/1‑68 (MQ=255) cTTGTACCAGTAGTCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGa > 1:2969400/1‑69 (MQ=255) cTTGTACCAGTAGTCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGa > 1:2338927/1‑69 (MQ=255) cTTGTACCAGTAGTCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGa > 1:537404/1‑69 (MQ=255) cTTGTACCAGTAGTCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGa > 1:801063/1‑69 (MQ=255) cTTGTACCAGTAGTCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGa > 1:1508768/1‑69 (MQ=255) gtCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACg > 1:273258/1‑55 (MQ=255) gtCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACg > 1:931623/1‑55 (MQ=255) gtCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACg > 1:277648/1‑55 (MQ=255) gtCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACg > 1:616966/1‑55 (MQ=255) gtCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACg > 1:3358979/1‑55 (MQ=255) gtCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACg > 1:1541850/1‑55 (MQ=255) gtCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCTCGGTAagc > 1:2917857/1‑69 (MQ=255) gtCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAagc < 1:2260922/69‑1 (MQ=255) gtCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAagc < 1:355885/69‑1 (MQ=255) gtCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAagc < 1:1116979/69‑1 (MQ=255) gtCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAagc < 1:2414322/69‑1 (MQ=255) gtCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAagc < 1:2614879/69‑1 (MQ=255) gtCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAagc > 1:2881214/1‑69 (MQ=255) gtCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAagc < 1:1695065/69‑1 (MQ=255) gtCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAagc > 1:1550914/1‑69 (MQ=255) gtCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAagc > 1:846259/1‑69 (MQ=255) gtCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAagc < 1:141516/69‑1 (MQ=255) cAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAgcagca < 1:548111/68‑1 (MQ=255) aaTGCCAGCTTCCACCGCAACACGAGCGGTAACCGCTTTCGGCAGTACGGATTCACgg < 1:912374/58‑1 (MQ=255) aaTGCCAGCTTCCACCGCAACACGAGCGGTAACCGCTTTCGGCAGTACGGATTCACgg < 1:1135545/58‑1 (MQ=255) aaTGCCAGCTTCCACCGCAACACGAGCGGTAACCGCTTTCGGCAGTACGGATTCACgg < 1:126422/58‑1 (MQ=255) aaTGCCAGCTTCCACCGCAACACGAGCGGTAACCGCTTTCGGCAGTACGGATTCACgg < 1:1290308/58‑1 (MQ=255) aaTGCCAGCTTCCACCGCAACACGAGCGGTAACCGCTTTCGGCAGTACGGATTCACgg < 1:302468/58‑1 (MQ=255) aaTGCCAGCTTCCACCGCAACACGAGCGGTAACCGCTTTCGGCAGTACGGATTCACgg < 1:1101994/58‑1 (MQ=255) aaTGCCAGCTTCCACCGCAACACGAGCGGTAACCGCTTTCGGCAGTACGGATTCACgg < 1:3038477/58‑1 (MQ=255) aaTGCCAGCTTCCACCGCAACACGAGCGGTAACCGCTTTCGGCAGTACGGATTCACgg < 1:3060681/58‑1 (MQ=255) aaTGCCAGCTTCCACCGCAACACGAGCGGTAACCGCTTTCGGCAGTACGGATTCACgg < 1:1372726/58‑1 (MQ=255) aaTGCCAGCTTCCACCGCAACACGAGCGGTAACCGCTTTCGGCAGTACGGATTCACgg < 1:3113316/58‑1 (MQ=255) aaTGCCAGCTTCCACCGCAACACGAGCGGTAACCGCTTTCGGCAGTACGGATTCACgg < 1:2399533/58‑1 (MQ=255) aaTGCCAGCTTCCACCGCAACACGAGCGGTAACCGCTTTCGGCAGTACGGATTCACgg < 1:3182777/58‑1 (MQ=255) aaTGCCAGCTTCCACCGCAACACGAGCGGTAACCGCTTTCGGCAGTACGGATTCACgg < 1:3324875/58‑1 (MQ=255) aaTGCCAGCTTCCACCGCAACACGAGCGGTAACCGCTTTCGGCAGTACGGATTCACgg < 1:2825118/58‑1 (MQ=255) aaTGCCAGCTTCCACCGCAACACGAGCGGTAACCGCTTTCGGCAGTACGGATTCACgg < 1:221076/58‑1 (MQ=255) aaTGCCAGCTTCCACCGCAACACGAGCGGTAACCGCTTTCGGCAGTACGGATTCACgg < 1:2194653/58‑1 (MQ=255) aaTGCCAGCTTCCACCGCAACACGAGCGGTAACCGCTTTCGGCAGTACGGATTCACgg < 1:2175773/58‑1 (MQ=255) aaTGCCAGCTTCCACCGCAACACGAGCGGTAACCGCTTTCGGCAGTACGGATTCACgg < 1:344441/58‑1 (MQ=255) aaTGCCAGCTTCCACCGCAACACGAGCGGTAACCGCTTTCGGCAGTACGGATTCACgg < 1:200444/58‑1 (MQ=255) aaTGCCAGCTTCCACCGCAACACGAGCGGTAACCGCTTTCGGCAGTACGGATTCACgg < 1:1855013/58‑1 (MQ=255) aTACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATcc > 1:2490924/1‑69 (MQ=255) aTACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATcc > 1:2072594/1‑69 (MQ=255) cccGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTa > 1:2730152/1‑43 (MQ=255) cccGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGAtt > 1:2869574/1‑49 (MQ=255) cccGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTg > 1:1454706/1‑68 (MQ=255) cccGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTg > 1:2080867/1‑68 (MQ=255) cccGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGc > 1:2916187/1‑69 (MQ=255) cccGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGc > 1:2590009/1‑69 (MQ=255) cccGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGc > 1:888251/1‑69 (MQ=255) cccGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGc > 1:845724/1‑69 (MQ=255) cccGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGc > 1:714199/1‑69 (MQ=255) cccGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGc > 1:3333983/1‑69 (MQ=255) cccGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGc > 1:350275/1‑69 (MQ=255) cccGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGc > 1:2273467/1‑69 (MQ=255) cccGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGc > 1:1732384/1‑69 (MQ=255) cccGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGc > 1:1815986/1‑69 (MQ=255) cccGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGc > 1:1982753/1‑69 (MQ=255) cccGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGc > 1:2400430/1‑69 (MQ=255) cccGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGc > 1:145970/1‑69 (MQ=255) cccGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGc > 1:2561034/1‑69 (MQ=255) cccGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGc > 1:1271673/1‑69 (MQ=255) cccGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGc > 1:2642203/1‑69 (MQ=255) cccGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGc > 1:1192939/1‑69 (MQ=255) cccGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGc > 1:1188079/1‑69 (MQ=255) cccGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGAATCACGGTAAGCAGCATCCTGc > 1:337571/1‑69 (MQ=255) cccGCTTCTACAACAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAACATCCTGc > 1:1476233/1‑69 (MQ=255) gCTTCCACCGCAACACGAGCGGTAACCGCTTTCGGCAGTACGGATTCCCGGTAagccgcg > 1:1243044/1‑59 (MQ=255) gCTTCCACCGCAACACGAGCGGTAACCGCTTTCGGCAGTACGGATTCACGGTAagccgcg > 1:1455748/1‑59 (MQ=255) gCTTCCACCGCAACACGAGCGGTAACCGCTTTCGGCAGTACGGATTCACGGTAagccgcg > 1:94475/1‑59 (MQ=255) gCTTCCACCGCAACACGAGCGGTAACCGCTTTCGGCAGTACGGATTCACGGTAagccgcg > 1:77829/1‑59 (MQ=255) gCTTCCACCGCAACACGAGCGGTAACCGCTTTCGGCAGTACGGATTCACGGTAagccgcg > 1:769279/1‑59 (MQ=255) gCTTCCACCGCAACACGAGCGGTAACCGCTTTCGGCAGTACGGATTCACGGTAagccgcg > 1:726337/1‑59 (MQ=255) gCTTCCACCGCAACACGAGCGGTAACCGCTTTCGGCAGTACGGATTCACGGTAagccgcg > 1:706339/1‑59 (MQ=255) gCTTCCACCGCAACACGAGCGGTAACCGCTTTCGGCAGTACGGATTCACGGTAagccgcg > 1:68858/1‑59 (MQ=255) gCTTCCACCGCAACACGAGCGGTAACCGCTTTCGGCAGTACGGATTCACGGTAagccgcg > 1:1807444/1‑59 (MQ=255) gCTTCCACCGCAACACGAGCGGTAACCGCTTTCGGCAGTACGGATTCACGGTAagccgcg > 1:361510/1‑59 (MQ=255) gCTTCCACCGCAACACGAGCGGTAACCGCTTTCGGCAGTACGGATTCACGGTAagccgcg > 1:184328/1‑59 (MQ=255) gCTTCCACCGCAACACGAGCGGTAACCGCTTTCGGCAGTACGGATTCACGGTAagccgcg > 1:1854725/1‑59 (MQ=255) gCTTCCACCGCAACACGAGCGGTAACCGCTTTCGGCAGTACGGATTCACGGTAagccgcg > 1:1980205/1‑59 (MQ=255) gCTTCCACCGCAACACGAGCGGTAACCGCTTTCGGCAGTACGGATTCACGGTAagccgcg > 1:3065635/1‑59 (MQ=255) gCTTCCACCGCAACACGAGCGGTAACCGCTTTCGGCAGTACGGATTCACGGTAagccgcg > 1:2565933/1‑59 (MQ=255) gCTTCCACCGCAACACGAGCGGTAACCGCTTTCGGCAGTACGGATTCACGGTAagccgcg > 1:2597659/1‑59 (MQ=255) gCTTCCACCGCAACACGAGCGGTAACCGCTTTCGGCAGTACGGATTCACGGTAagccgcg > 1:2965961/1‑59 (MQ=255) gCTTCCACCGCAACACGAGCGGTAACCGCTTTCGGCAGTACGGATTCACGGTAagccgcg > 1:2671674/1‑59 (MQ=255) gCTTCCACCGCAACACGAGCGGTAACCGCTTTCGGCAGTACGGATTCACGGTAagccgcg > 1:2863331/1‑59 (MQ=255) gCTTCCACCGCAACACGAGCGGTAACCGCTTTCGGCAGTACGGATTCACAGTAagccgcg > 1:1516350/1‑59 (MQ=255) tCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCa < 1:195973/69‑1 (MQ=255) tCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCa < 1:2871655/69‑1 (MQ=255) gCAACACGAGCGGTAACCGCTTTCGGCAGTACGGATTCACgg < 1:152796/42‑1 (MQ=37) aaCACGAGCGGTAACCGCTTTCGGCAGTACGGATTCACgg < 1:2272232/40‑1 (MQ=37) | CGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCA > minE/1966742‑1966863 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |