| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,967,112 | A→G | 48.1% | G506G (GGT→GGC) | tktA ← | transketolase 1, thiamin‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,967,112 | 0 | A | G | 48.1% | ‑3.5 / 44.9 | 52 | G506G (GGT→GGC) | tktA | transketolase 1, thiamin‑binding |
| Reads supporting (aligned to +/- strand): ref base A (11/16); new base G (8/17); total (19/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.74e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTCGTTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGT > minE/1967045‑1967177 | ttcgttcCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAacac < 1:872078/69‑1 (MQ=255) cgttcCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGCCGCTCAACACCg > 1:1713725/1‑69 (MQ=255) cgttcCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCg > 1:2827431/1‑69 (MQ=255) cgttcCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCg > 1:2296874/1‑69 (MQ=255) cgttcCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCg > 1:2249607/1‑69 (MQ=255) cgttcCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCg > 1:671451/1‑69 (MQ=255) cgttcCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCg > 1:445993/1‑69 (MQ=255) cgttcCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCg > 1:1864108/1‑69 (MQ=255) ccAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCTTCCTGACGCTCAACACCGTATTTCCAcgcg < 1:1168562/68‑1 (MQ=255) ccAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCGTATTTCCAcgcg < 1:1816989/68‑1 (MQ=255) ccAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCGTATTTCCAcgcg < 1:3121681/68‑1 (MQ=255) ccAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCGTATTTCCAcgcg < 1:3153931/68‑1 (MQ=255) ccAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCGTATTTCCAcgcg < 1:2129643/68‑1 (MQ=255) ccAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCGTATTTCCAcgcg < 1:333217/68‑1 (MQ=255) ccAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCGTATTTCCAcgcg < 1:390970/68‑1 (MQ=255) ccAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCGTATTTCCAcgcg < 1:1813800/68‑1 (MQ=255) ccAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCGTATTTCCAcgcg < 1:395459/68‑1 (MQ=255) ccAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCGTATTTCCAcgcg < 1:1459535/68‑1 (MQ=255) ccAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCGTATTTCCAcgcg < 1:1302308/68‑1 (MQ=255) ccAGGTTCTGACGGGAGAGGAGCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCGTATTTCCAcgcg < 1:1610706/68‑1 (MQ=255) gATCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCGTATTTCCAcgcg < 1:867034/49‑1 (MQ=255) tGCGGTCGGGCCGTCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACg < 1:125759/68‑1 (MQ=255) gCGGTCGGGCCGTCCTGACGCTCAACACCGTATTTCCAcgcg < 1:2161110/42‑1 (MQ=255) gTCGGGCCGTCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGc < 1:3253154/69‑1 (MQ=255) tCAACGCCGTATTTCCACGCTACTGCGGATTCAACCTTGTCACACGGACGCCATGTAGACATGTTCggg < 1:1402776/69‑1 (MQ=255) tCAACGCCGTATTTCCACGCTACTGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCggg < 1:2459884/69‑1 (MQ=255) tCAACGCCGTATTTCCACGCTACTGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCggg < 1:369489/69‑1 (MQ=255) tCAACGCCGTATTTCCACGCTACTGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCggg < 1:72749/69‑1 (MQ=255) tCAACGCCGTATTTCCACGCTACTGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCggg < 1:328527/69‑1 (MQ=255) tCAACGCCGTATTTCCACGCTACTGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCggg < 1:3183661/69‑1 (MQ=255) tCAACGCCGTATTTCCACGCTACTGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCggg < 1:2943917/69‑1 (MQ=255) tCAACGCCGTATTTCCACGCTACTGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCggg < 1:1133656/69‑1 (MQ=255) tCAACGCCGTATTTCCACGCTACTGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCggg < 1:2797177/69‑1 (MQ=255) tCAACGCCGTATTTCCACGCTACTGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCggg < 1:100618/69‑1 (MQ=255) tCAACGCCGTATTTCCACGCTACTGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCggg < 1:2450057/69‑1 (MQ=255) tCAACGCCGTATTTCCACGCTACTGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCggg < 1:1355442/69‑1 (MQ=255) tCAACGCCGTATTTCCACGCTACTGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCggg < 1:13890/69‑1 (MQ=255) tCAACGCCGTATTTCCACGCTACTGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCggg < 1:1615258/69‑1 (MQ=255) tCAACGCCGTATTTCCACGCTACTGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCggg < 1:1464835/69‑1 (MQ=255) tCAACGCCGTATTTCCACGCTACTGCGGATTCAACCTGGGCACACGGACGCCATGTAGACATGTTCggg < 1:1980384/69‑1 (MQ=255) cAACGCCGTATTTCCACGCTACTGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCggg < 1:1453415/68‑1 (MQ=255) cAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCggg > 1:3068782/1‑68 (MQ=255) cAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCggg > 1:2326213/1‑68 (MQ=255) cAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCggg > 1:697245/1‑68 (MQ=255) cAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCggg > 1:51638/1‑68 (MQ=255) aaCGCCGTATTTCCACGCTACTGCGGATTCAACCTGGTcac > 1:824840/1‑41 (MQ=37) aaCGCCGTATTTCCACGCTACTGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGt > 1:1898502/1‑69 (MQ=255) aaCGCCGTATTTCCACGCTACTGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGt > 1:595338/1‑69 (MQ=255) aaCGCCGTATTTCCACGCTACTGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGt > 1:522313/1‑69 (MQ=255) aaCGCCGTATTTCCACGCTACTGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGt > 1:287647/1‑69 (MQ=255) aaCGCCGTATTTCCACGCTACTGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGt > 1:1956869/1‑69 (MQ=255) aaCGCCGTATTTCCACGCTACTGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGt > 1:3232132/1‑69 (MQ=255) aaCGCCGTATTTCCACGCTACTGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGt > 1:2963549/1‑69 (MQ=255) acCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACg < 1:2433625/41‑1 (MQ=255) | TTCGTTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGT > minE/1967045‑1967177 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |