| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,967,130 | C→T | 81.0% | A500A (GCG→GCA) | tktA ← | transketolase 1, thiamin‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,967,130 | 0 | C | T | 81.0% | 66.9 / 8.4 | 47 | A500A (GCG→GCA) | tktA | transketolase 1, thiamin‑binding |
| Reads supporting (aligned to +/- strand): ref base C (4/5); new base T (21/17); total (25/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.15e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.13e-01 | |||||||||||
TGCGGTCGGGCCGTCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGC > minE/1967085‑1967192 | tGCGGTCGGGCCGTCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACg < 1:125759/68‑1 (MQ=255) gTCGGGCCGTCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGc < 1:3253154/69‑1 (MQ=255) tCAACGCCGTATTTCCACGCTACTGCGGATTCAACCTTGTCACACGGACGCCATGTAGACATGTTCggg < 1:1402776/69‑1 (MQ=255) tCAACGCCGTATTTCCACGCTACTGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCggg < 1:100618/69‑1 (MQ=255) tCAACGCCGTATTTCCACGCTACTGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCggg < 1:2450057/69‑1 (MQ=255) tCAACGCCGTATTTCCACGCTACTGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCggg < 1:2459884/69‑1 (MQ=255) tCAACGCCGTATTTCCACGCTACTGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCggg < 1:2797177/69‑1 (MQ=255) tCAACGCCGTATTTCCACGCTACTGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCggg < 1:2943917/69‑1 (MQ=255) tCAACGCCGTATTTCCACGCTACTGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCggg < 1:3183661/69‑1 (MQ=255) tCAACGCCGTATTTCCACGCTACTGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCggg < 1:328527/69‑1 (MQ=255) tCAACGCCGTATTTCCACGCTACTGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCggg < 1:369489/69‑1 (MQ=255) tCAACGCCGTATTTCCACGCTACTGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCggg < 1:1615258/69‑1 (MQ=255) tCAACGCCGTATTTCCACGCTACTGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCggg < 1:1464835/69‑1 (MQ=255) tCAACGCCGTATTTCCACGCTACTGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCggg < 1:13890/69‑1 (MQ=255) tCAACGCCGTATTTCCACGCTACTGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCggg < 1:72749/69‑1 (MQ=255) tCAACGCCGTATTTCCACGCTACTGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCggg < 1:1355442/69‑1 (MQ=255) tCAACGCCGTATTTCCACGCTACTGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCggg < 1:1133656/69‑1 (MQ=255) tCAACGCCGTATTTCCACGCTACTGCGGATTCAACCTGGGCACACGGACGCCATGTAGACATGTTCggg < 1:1980384/69‑1 (MQ=255) cAACGCCGTATTTCCACGCTACTGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCggg < 1:1453415/68‑1 (MQ=255) cAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCggg > 1:51638/1‑68 (MQ=255) cAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCggg > 1:697245/1‑68 (MQ=255) cAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCggg > 1:3068782/1‑68 (MQ=255) cAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCggg > 1:2326213/1‑68 (MQ=255) aaCGCCGTATTTCCACGCTACTGCGGATTCAACCTGGTcac > 1:824840/1‑41 (MQ=37) aaCGCCGTATTTCCACGCTACTGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGt > 1:287647/1‑69 (MQ=255) aaCGCCGTATTTCCACGCTACTGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGt > 1:2963549/1‑69 (MQ=255) aaCGCCGTATTTCCACGCTACTGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGt > 1:595338/1‑69 (MQ=255) aaCGCCGTATTTCCACGCTACTGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGt > 1:522313/1‑69 (MQ=255) aaCGCCGTATTTCCACGCTACTGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGt > 1:3232132/1‑69 (MQ=255) aaCGCCGTATTTCCACGCTACTGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGt > 1:1956869/1‑69 (MQ=255) aaCGCCGTATTTCCACGCTACTGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGt > 1:1898502/1‑69 (MQ=255) acCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACg < 1:2433625/41‑1 (MQ=255) cgctACTGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTCACGCGCAGAGAAGc > 1:1131486/1‑69 (MQ=255) cgctACTGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTCACGCGCAGAGAAGc > 1:813678/1‑69 (MQ=255) cgctACTGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTCACGCGCAGAGAAGc > 1:1385953/1‑69 (MQ=255) cgctACTGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTCACGCGCAGAGAAGc > 1:678950/1‑69 (MQ=255) cgctACTGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTCACGCGCAGAGAAGc > 1:1757655/1‑69 (MQ=255) cgctACTGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTCACGCGCAGAGAAGc > 1:430837/1‑69 (MQ=255) cgctACTGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTCACGCGCAGAGAAGc > 1:420151/1‑69 (MQ=255) cgctACTGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTCACGCGCAGAGAAGc > 1:2072890/1‑69 (MQ=255) cgctACTGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTCACGCGCAGAGAAGc > 1:3160833/1‑69 (MQ=255) cgctACTGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTCACGCGCAGAGAAGc > 1:2131289/1‑69 (MQ=255) cgctACTGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTCACGCGCAGAGAAGc > 1:2272553/1‑69 (MQ=255) cgctACTGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTCACGCGCAGAGAAGc > 1:2287582/1‑69 (MQ=255) cgctACTGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTCACGCGCAGAGAAGc > 1:269419/1‑69 (MQ=255) cgcgACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGc < 1:2023194/69‑1 (MQ=255) cgcgACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGc < 1:1498204/69‑1 (MQ=255) | TGCGGTCGGGCCGTCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGC > minE/1967085‑1967192 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |