| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,967,301 | G→A | 20.8% | N443N (AAC→AAT) | tktA ← | transketolase 1, thiamin‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,967,301 | 0 | G | A | 20.8% | 213.3 / 39.6 | 143 | N443N (AAC→AAT) | tktA | transketolase 1, thiamin‑binding |
| Reads supporting (aligned to +/- strand): ref base G (64/49); new base A (17/13); total (81/62) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.66e-01 | |||||||||||
Only 100 of 143 total aligned reads displayed.
GAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCAGGGA > minE/1967242‑1967366 | gAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCATTACGTGCg < 1:3378603/69‑1 (MQ=255)gAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCATTACGTGCg < 1:3122008/69‑1 (MQ=255)gAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCATTACGTGCg < 1:2738253/69‑1 (MQ=255)gAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCATTACGTGCg < 1:269923/69‑1 (MQ=255)gAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCATTACGTGCg < 1:198128/69‑1 (MQ=255)gAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCATTACGTGCg < 1:2479849/69‑1 (MQ=255)gAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCATTACGTGCg < 1:1136711/69‑1 (MQ=255)gAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCATTACGTGCg < 1:2444473/69‑1 (MQ=255)gAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCATTACGTGCg < 1:2360904/69‑1 (MQ=255)gAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCATTACGTGCg < 1:768507/69‑1 (MQ=255) ggcGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCGt < 1:2156557/66‑1 (MQ=255) gTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCGt < 1:3363798/68‑1 (MQ=255) gTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCGt < 1:2626089/68‑1 (MQ=255) gtAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCGTATTCCACGaa < 1:1168365/69‑1 (MQ=255) aCCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCGt < 1:2041417/55‑1 (MQ=255) aCCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCGTATTCCACGAACATc > 1:1490218/1‑69 (MQ=255) aCCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCGTATTCCACGAACATc > 1:277926/1‑69 (MQ=255) aCCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCGTATTCCACGAACATc > 1:1538275/1‑69 (MQ=255) ccATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCGTATTCCACGAACATCa < 1:2071970/69‑1 (MQ=255) ccATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCGTATTCCACGAACATCa < 1:1794324/69‑1 (MQ=255) aCCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCGTATTCCACGAACATCAGGAAg > 1:1486836/1‑69 (MQ=255) aCCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCGTATTCCACGAACATCAGGAAg > 1:2282488/1‑69 (MQ=255) aCCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCGTATTCCACGAACATCAGGAAg > 1:2311964/1‑69 (MQ=255) aCCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCGTATTCCACGAACATCAGGAAg > 1:500915/1‑69 (MQ=255) aCCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCGTATTCCACGAACATCAGGAAg > 1:370053/1‑69 (MQ=255) aCCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCGTATTCCACGAACATCAGGAAg > 1:1256459/1‑69 (MQ=255) aCCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCGTATTCCACGAACATCAGGAAg > 1:2737966/1‑69 (MQ=255) aCCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCGTATTCCACGAACATCAGGAAg > 1:3293296/1‑69 (MQ=255) aCCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCGTATTCCACGAACATCAGGAAg > 1:2845609/1‑69 (MQ=255) gACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTgg > 1:1345938/1‑69 (MQ=255) gACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTgg > 1:1375132/1‑69 (MQ=255) gACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTgg > 1:456307/1‑69 (MQ=255) gACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTgg > 1:3048372/1‑69 (MQ=255) gACGCTGTTTCATCAGCGCAGCCATACGTACGGAGTTACGTGCGTATTCCACGAACATCAGGAAGGTgg > 1:3375279/1‑69 (MQ=255) gCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGa < 1:2712218/67‑1 (MQ=255) gCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGa < 1:3319299/67‑1 (MQ=255) gCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGa < 1:36107/67‑1 (MQ=255) gCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGa < 1:238439/67‑1 (MQ=255) gCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGa < 1:2896500/67‑1 (MQ=255) gCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGa < 1:921454/67‑1 (MQ=255) gCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGa < 1:644712/67‑1 (MQ=255) gCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGa < 1:2144938/67‑1 (MQ=255) gCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGa < 1:1952468/67‑1 (MQ=255) gCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGa < 1:1050743/67‑1 (MQ=255) tGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGgtg < 1:1680033/69‑1 (MQ=255) atcaGCGCAGCCATACGTACGGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACgg < 1:1280915/68‑1 (MQ=255) atcaGCGCAGCCATACGTACGGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACgg < 1:1666147/68‑1 (MQ=255) atcaGCGCAGCCATACGTACGGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACgg < 1:638361/68‑1 (MQ=255) atcaGCGCAGCCATACGTACGGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACgg < 1:516776/68‑1 (MQ=255) atcaGCGCAGCCATACGTACGGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACgg < 1:441632/68‑1 (MQ=255) tcaGCGCAGCCATACGTACGGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGc < 1:898456/68‑1 (MQ=255) gcAGCCATACGTACGGCGTTACGTGCGTATTCCACGaa < 1:2305669/38‑1 (MQ=255) cAGCCATACGTACGGCGTTACGTGCGTATTCCACGAACATCa < 1:565531/42‑1 (MQ=255) cAGCCATACGTACGGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACgg < 1:2060797/61‑1 (MQ=255) ccATACGTACGGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCAc < 1:3237480/69‑1 (MQ=255) ccATACGTACGGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCAc < 1:225568/69‑1 (MQ=255) ccATACGTACGGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCAc < 1:1532550/69‑1 (MQ=255) aTACGTACGGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGa < 1:12187/47‑1 (MQ=255) aTACGTACGGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCg < 1:467976/69‑1 (MQ=255) aTACGTACGGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCg < 1:1186083/69‑1 (MQ=255) acgtacgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACgg < 1:2047933/54‑1 (MQ=255) cgtacgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGc > 1:2834233/1‑69 (MQ=255) cgtacgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGc > 1:1232464/1‑69 (MQ=255) cgtacgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGc > 1:481113/1‑69 (MQ=255) cgtacgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGc > 1:774761/1‑69 (MQ=255) cgtacgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGc > 1:739081/1‑69 (MQ=255) cgtacgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGc > 1:2013352/1‑69 (MQ=255) cgtacgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGc > 1:1463850/1‑69 (MQ=255) cgtacgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGc > 1:2632517/1‑69 (MQ=255) cgtacgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGc > 1:1044099/1‑69 (MQ=255) cgtacgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGc > 1:1517445/1‑69 (MQ=255) cgtacgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGc > 1:1879283/1‑69 (MQ=255) cgtacgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGc > 1:3430074/1‑69 (MQ=255) cgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGc > 1:72575/1‑65 (MQ=255) cgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCa > 1:3287133/1‑66 (MQ=255) cgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCa > 1:395066/1‑66 (MQ=255) cgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCa > 1:559052/1‑66 (MQ=255) cgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCa > 1:684896/1‑66 (MQ=255) cgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCa > 1:864522/1‑66 (MQ=255) cgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCa > 1:1009842/1‑66 (MQ=255) cgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCa > 1:3351895/1‑66 (MQ=255) cgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCa > 1:1321400/1‑66 (MQ=255) cgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCa > 1:1432079/1‑66 (MQ=255) cgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCa > 1:2391771/1‑66 (MQ=255) cgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCa > 1:194863/1‑66 (MQ=255) cgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCa > 1:2955332/1‑66 (MQ=255) ggCATTACGTGCGTATTCCACGAACATCAGGAAGGTTGAGGTATACGGCAGGAACCCACCGTGCa > 1:2062800/1‑65 (MQ=25) ggCATTACGTGCGTATTCCACGAACATCAGGAAGGTTGAGGTATACGGCAGGAAACCa > 1:1128751/1‑58 (MQ=21) ggCATTACGTGCGTATTCCACGAACATCAGGAAGGTTGAGGTATACGGCAGGAAACCACCGTGc > 1:2418803/1‑64 (MQ=21) ggCATTACGTGCGTATTCCACGAACATCAGGAAGGTTGAGGTATACGGCAGGAAACCACCGTGCa > 1:2231823/1‑65 (MQ=25) ggCATTACGTGCGTATTCCACGAACATCAGGAAGGTTGAGGTATACGGCAGGAAACCACCGTGCa > 1:825122/1‑65 (MQ=25) ggCATTACGTGCGTATTCCACGAACATCAGGAAGGTTGAGGTATACGGCAGGAAACCACCGTGCa > 1:1754035/1‑65 (MQ=25) ggCATTACGTGCGTATTCCACGAACATCAGGAAGGTTGAGGTATACGGCAGGAAACCACCGTGCa > 1:3024757/1‑65 (MQ=25) ggCATTACGTGCGTATTCCACGAACATCAGGAAGGTTGAGGTATACGGCAGGAAACCACCGTGCa > 1:3038353/1‑65 (MQ=25) ggCATTACGTGCGTATTCCACGAACATCAGGAAGGTTGAGGTATACGGCAGGAAACCACCGTGCa > 1:3117644/1‑65 (MQ=25) ggCATTACGTGCGTATTCCACGAACATCAGGAAGGTTGAGGTATACGGCAGGAAACCACCGTGCa > 1:3199734/1‑65 (MQ=25) ggCATTACGTGCGTATTCCACGAACATCAGGAAGGTTGAGGTATACGGCAGGAAACCACCGTGCa > 1:106018/1‑65 (MQ=25) ggCATTACGTGCGTATTCCACGAACATCAGGAAGGTTGAGGTATACGGCAGGAAACCACCGTGCa > 1:1160558/1‑65 (MQ=25) ggCATTACGTGCGTATTCCACGAACATCAGGAAGGTTGAGGTATACGGCAGGAAACCACCGTGCAggg > 1:2326529/1‑68 (MQ=25) cGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCAGGga < 1:1012078/67‑1 (MQ=255) | GAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCAGGGA > minE/1967242‑1967366 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |