| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,967,334 | G→T | 27.2% | S432S (TCC→TCA) | tktA ← | transketolase 1, thiamin‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,967,334 | 0 | G | T | 27.2% | 203.4 / 64.6 | 128 | S432S (TCC→TCA) | tktA | transketolase 1, thiamin‑binding |
| Reads supporting (aligned to +/- strand): ref base G (50/43); new base T (17/18); total (67/61) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.92e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 136 total aligned reads displayed.
GACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCAGGGAGATACCGTTAGCAATCGCGGTCATACCGAA > minE/1967267‑1967396 | gACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTgg > 1:3410964/1‑69 (MQ=255)gACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTgg > 1:3048372/1‑69 (MQ=255)gACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTgg > 1:456307/1‑69 (MQ=255)gACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTgg > 1:871822/1‑69 (MQ=255)gACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTgg > 1:1345938/1‑69 (MQ=255)gACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTgg > 1:1375132/1‑69 (MQ=255)gACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTgg > 1:2368127/1‑69 (MQ=255)gACGCTGTTTCATCAGCGCAGCCATACGTACGGAGTTACGTGCGTATTCCACGAACATCAGGAAGGTgg > 1:3375279/1‑69 (MQ=255) gCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGa < 1:288571/67‑1 (MQ=255) gCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGa < 1:2896500/67‑1 (MQ=255) gCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGa < 1:2144938/67‑1 (MQ=255) gCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGa < 1:1209623/67‑1 (MQ=255) gCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGa < 1:3319299/67‑1 (MQ=255) tcatcaGCGCAGCCATACGTACGGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGgtg > 1:79461/1‑65 (MQ=255) tcatcaGCGCAGCCATACGTACGGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGgtg > 1:2049774/1‑65 (MQ=255) atcaGCGCAGCCATACGTACGGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACgg < 1:638361/68‑1 (MQ=255) atcaGCGCAGCCATACGTACGGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACgg < 1:3145879/68‑1 (MQ=255) atcaGCGCAGCCATACGTACGGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACgg < 1:1959942/68‑1 (MQ=255) atcaGCGCAGCCATACGTACGGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACgg < 1:1242708/68‑1 (MQ=255) atcaGCGCAGCCATACGTACGGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACgg < 1:3098766/68‑1 (MQ=255) atcaGCGCAGCCATACGTACGGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACgg < 1:1280915/68‑1 (MQ=255) atcaGCGCAGCCATACGTACGGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACgg < 1:1666147/68‑1 (MQ=255) tcaGCGCAGCCATACGTACGGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGc < 1:898456/68‑1 (MQ=255) cAGCCATACGTACGGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACgg < 1:2060797/61‑1 (MQ=255) ccATACGTACGGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCAc < 1:1501774/69‑1 (MQ=255) aTACGTACGGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGa < 1:12187/47‑1 (MQ=255) aTACGTACGGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCg < 1:467976/69‑1 (MQ=255) aTACGTACGGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCg < 1:1186083/69‑1 (MQ=255) cgtacgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGaa > 1:3241669/1‑59 (MQ=255) cgtacgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGc > 1:2714963/1‑69 (MQ=255) cgtacgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGc > 1:2393314/1‑69 (MQ=255) cgtacgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGc > 1:1879283/1‑69 (MQ=255) cgtacgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGc > 1:301095/1‑69 (MQ=255) cgtacgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGc > 1:739081/1‑69 (MQ=255) cgtacgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGc > 1:2632517/1‑69 (MQ=255) cgtacgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGc > 1:481113/1‑69 (MQ=255) cgtacgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGc > 1:2013352/1‑69 (MQ=255) cgtacgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGc > 1:1517445/1‑69 (MQ=255) cgtacgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGc > 1:1485918/1‑69 (MQ=255) cgtacgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGc > 1:1463850/1‑69 (MQ=255) cgtacgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGc > 1:456071/1‑69 (MQ=255) cgtacgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGc > 1:3430074/1‑69 (MQ=255) cgtacgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGc > 1:1044099/1‑69 (MQ=255) cgtacgGCGTTACGTGCGTATTCCACGAACATAAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGc > 1:1310410/1‑69 (MQ=255) cgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCa > 1:1009842/1‑66 (MQ=255) cgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCa > 1:3287133/1‑66 (MQ=255) cgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCa > 1:2955332/1‑66 (MQ=255) cgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCa > 1:3351895/1‑66 (MQ=255) cgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCa > 1:549293/1‑66 (MQ=255) cgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCa > 1:559052/1‑66 (MQ=255) cgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCa > 1:395066/1‑66 (MQ=255) cgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCa > 1:624917/1‑66 (MQ=255) cgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCa > 1:26556/1‑66 (MQ=255) cgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCa > 1:2391771/1‑66 (MQ=255) cgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCa > 1:684896/1‑66 (MQ=255) cgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCa > 1:1432079/1‑66 (MQ=255) cgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCa > 1:2809697/1‑66 (MQ=255) cgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCa > 1:194863/1‑66 (MQ=255) cgGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCa > 1:864522/1‑66 (MQ=255) cgGAGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCa > 1:1743768/1‑66 (MQ=255) ggCATTACGTGCGTATTCCACGAACATCAGGAAGGTTGAGGTATACGGCAGGAACCCACCGTGCa > 1:2062800/1‑65 (MQ=25) ggCATTACGTGCGTATTCCACGAACATCAGGAAGGTTGAGGTATACGGCAGGAAACCa > 1:1128751/1‑58 (MQ=21) ggCATTACGTGCGTATTCCACGAACATCAGGAAGGTTGAGGTATACGGCAGGAAACCACCGTGCa > 1:825122/1‑65 (MQ=25) ggCATTACGTGCGTATTCCACGAACATCAGGAAGGTTGAGGTATACGGCAGGAAACCACCGTGCa > 1:106018/1‑65 (MQ=25) ggCATTACGTGCGTATTCCACGAACATCAGGAAGGTTGAGGTATACGGCAGGAAACCACCGTGCa > 1:1160558/1‑65 (MQ=25) ggCATTACGTGCGTATTCCACGAACATCAGGAAGGTTGAGGTATACGGCAGGAAACCACCGTGCa > 1:1754035/1‑65 (MQ=25) ggCATTACGTGCGTATTCCACGAACATCAGGAAGGTTGAGGTATACGGCAGGAAACCACCGTGCa > 1:3199734/1‑65 (MQ=25) ggCATTACGTGCGTATTCCACGAACATCAGGAAGGTTGAGGTATACGGCAGGAAACCACCGTGCa > 1:2231823/1‑65 (MQ=25) ggCATTACGTGCGTATTCCACGAACATCAGGAAGGTTGAGGTATACGGCAGGAAACCACCGTGCa > 1:3038353/1‑65 (MQ=25) ggCATTACGTGCGTATTCCACGAACATCAGGAAGGTTGAGGTATACGGCAGGAAACCACCGTGCa > 1:3024757/1‑65 (MQ=25) ggCATTACGTGCGTATTCCACGAACATCAGGAAGGTTGAGGTATACGGCAGGAAACCACCGTGCAggg > 1:2326529/1‑68 (MQ=25) gCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCAGGga < 1:942403/68‑1 (MQ=255) gCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCAGGga < 1:2258935/68‑1 (MQ=255) cGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCAGGga < 1:1012078/67‑1 (MQ=255) cGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCAGGGAGATACCGTTa > 1:1551449/1‑68 (MQ=255) cGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCAGGGAGATACCGTTa > 1:2942826/1‑68 (MQ=255) cGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCAGGGAGATACCGTTa > 1:2939075/1‑68 (MQ=255) cGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCAGGGAGATACCGTTa > 1:730589/1‑68 (MQ=255) cGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCAGGGAGATACCGTTa > 1:2703656/1‑68 (MQ=255) aTTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCAGGGAGATACCGTTAGCaa < 1:509513/69‑1 (MQ=255) aTTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCAGGGAGATACCGTTAGCaa < 1:2413867/69‑1 (MQ=255) aTTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCAGGGAGATACCGTTAGCaa < 1:2351170/69‑1 (MQ=255) ttCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCAGGGAGATACCGTTAGCAAt > 1:1395638/1‑69 (MQ=255) ttCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCAGGGAGATACCGTTAGCAAt > 1:873325/1‑69 (MQ=255) ttCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCAGGGAGATACCGTTAGCAAt > 1:2183218/1‑69 (MQ=255) ttCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCAGGGAGATACCGTTAGCAAt > 1:770545/1‑69 (MQ=255) ccACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCAGGGAGATACCGTTAGCAATcg > 1:2292928/1‑69 (MQ=255) aaCATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCTGGGAGATACCGTTAGCaa < 1:3328905/61‑1 (MQ=255) aGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCAGGGAGATACCGTTAGCAATCGCGGTCATACCg < 1:712426/69‑1 (MQ=255) gAAGGTTGAGGTATACGGCAGGAAACCACCGTGCAGGGAGATACCGTTGGCAATCGCAGTCATACCGaa < 1:617727/69‑1 (MQ=255) gAAGGTTGAGGTATACGGCAGGAAACCACCGTGCAGGGAGATACCGTTGGCAATCGCAGTCATACCGaa < 1:520789/69‑1 (MQ=255) gAAGGTTGAGGTATACGGCAGGAAACCACCGTGCAGGGAGATACCGTTGGCAATCGCAGTCATACCGaa < 1:501522/69‑1 (MQ=255) gAAGGTTGAGGTATACGGCAGGAAACCACCGTGCAGGGAGATACCGTTGGCAATCGCAGTCATACCGaa < 1:296722/69‑1 (MQ=255) gAAGGTTGAGGTATACGGCAGGAAACCACCGTGCAGGGAGATACCGTTGGCAATCGCAGTCATACCGaa < 1:1996775/69‑1 (MQ=255) gAAGGTTGAGGTATACGGCAGGAAACCACCGTGCAGGGAGATACCGTTGGCAATCGCAGTCATACCGaa < 1:1889498/69‑1 (MQ=255) gAAGGTTGAGGTATACGGCAGGAAACCACCGTGCAGGGAGATACCGTTGGCAATCGCAGTCATACCGaa < 1:1671513/69‑1 (MQ=255) gAAGGTTGAGGTATACGGCAGGAAACCACCGTGCAGGGAGATACCGTTGGCAATCGCAGTCATACCGaa < 1:2998896/69‑1 (MQ=255) gAAGGTTGAGGTATACGGCAGGAAACCACCGTGCAGGGAGATACCGTTGGCAATCGCAGTCATACCGaa < 1:3280343/69‑1 (MQ=255) gAAGGTTGAGGTATACGGCAGGAAACCACCGTGCAGGGAGATACCGTTGGCAATCGCAGTCATACCGaa < 1:406813/69‑1 (MQ=255) gAAGGTTGAGGTATACGGCAGGAAACCACCGTGCAGGGAGATACCGTTGGCAATCGCAGTCATACCGaa < 1:944055/69‑1 (MQ=255) | GACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCGTATTCCACGAACATCAGGAAGGTGGAGGTGTACGGCAGGAAGCCACCGTGCAGGGAGATACCGTTAGCAATCGCGGTCATACCGAA > minE/1967267‑1967396 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |