| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,967,400 | G→A | 51.9% | R410R (CGC→CGT) | tktA ← | transketolase 1, thiamin‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,967,400 | 0 | G | A | 51.9% | 0.5 / 96.5 | 106 | R410R (CGC→CGT) | tktA | transketolase 1, thiamin‑binding |
| Reads supporting (aligned to +/- strand): ref base G (23/28); new base A (12/43); total (35/71) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.35e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 134 total aligned reads displayed.
GGTGTACGGCAGGAAGCCACCGTGCAGGGAGATACCGTTAGCAATCGCGGTCATACCGAACTCGCGAACACCGTAGTGGATGTAGTTACCCGCAGCATCTTCGTTGATTGCTTTAGAACCAG > minE/1967337‑1967458 | ggTGTACGGCAGGAAGCCACCGTGCAGGGAGATACCGTTAGCAATCGCGGTCATACCGAACTCGCGAac > 1:2326937/1‑69 (MQ=255)ggTGTACGGCAGGAAGCCACCGTGCAGGGAGATACCGTTAGCAATCGCGGTCATACCGAACTCGCGAac > 1:505274/1‑69 (MQ=255)ggTATACGGCAGGAAACCACCGTGCAGGGAGATACCGTTGGCAATCGCAGTCATACCGAATTcacgta < 1:1060310/68‑3 (MQ=21)ggTATACGGCAGGAAACCACCGTGCAGGGAGATACCGTTGGCAATCGCAGTCATACCGAATTcacgta < 1:2072864/68‑3 (MQ=21)ggTATACGGCAGGAAACCACCGTGCAGGGAGATACCGTTGGCAATCGCAGTCATACCGAATTcacgta < 1:3017136/68‑3 (MQ=21)ggTATACGGCAGGAAACCACCGTGCAGGGAGATACCGTTGGCAATCGCAGTCATACCGAATTcacgta < 1:2029035/68‑3 (MQ=21)ggTATACGGCAGGAAACCACCGTGCAGGGAGATACCGTTGGCAATCGCAGTCATACCGAATTcacgta < 1:3303047/68‑3 (MQ=21)ggTATACGGCAGGAAACCACCGTGCAGGGAGATACCGTTGGCAATCGCAGTCATACCGAATTcacgta < 1:1989937/68‑3 (MQ=21)ggTATACGGCAGGAAACCACCGTGCAGGGAGATACCGTTGGCAATCGCAGTCATACCGAATTcacgta < 1:384504/68‑3 (MQ=21)ggTATACGGCAGGAAACCACCGTGCAGGGAGATACCGTTGGCAATCGCAGTCATACCGAATTcacgta < 1:1917442/68‑3 (MQ=21)ggTATACGGCAGGAAACCACCGTGCAGGGAGATACCGTTGGCAATCGCAGTCATACCGAATTcacgta < 1:1768548/68‑3 (MQ=21)ggTATACGGCAGGAAACCACCGTGCAGGGAGATACCGTTGGCAATCGCAGTCATACCGAATTcacgta < 1:391417/68‑3 (MQ=21)ggTATACGGCAGGAAACCACCGTGCAGGGAGATACCGTTGGCAATCGCAGTCATACCGAATTcacgta < 1:1684888/68‑3 (MQ=21)ggTATACGGCAGGAAACCACCGTGCAGGGAGATACCGTTGGCAATCGCAGTCATACCGAATTcacgta < 1:470258/68‑3 (MQ=21)ggTATACGGCAGGAAACCACCGTGCAGGGAGATACCGTTGGCAATCGCAGTCATACCGAATTcacgta < 1:2773791/68‑3 (MQ=21)ggTATACGGCAGGAAACCACCGTGCAGGGAGATACCGTTGGCAATCGCAGTCATACCGAATTcacgta < 1:541657/68‑3 (MQ=21)ggTATACGGCAGGAAACCACCGTGCAGGGAGATACCGTTGGCAATCGCAGTCATACCGAATTcacgta < 1:582793/68‑3 (MQ=21)ggTATACGGCAGGAAACCACCGTGCAGGGAGATACCGTTGGCAATCGCAGTCATACCGAATTcacgta < 1:1303948/68‑3 (MQ=21)ggTATACGGCAGGAAACCACCGTGCAGGGAGATACCGTTGGCAATCGCAGTCATACCGAATTcacgta < 1:1569656/68‑3 (MQ=21)ggTATACGGCAGGAAACCACCGTGCAGGGAGATACCGTTGGCAATCGCAGTCATACCGAATTcacgta < 1:829233/68‑3 (MQ=21)ggTATACGGCAGGAAACCACCGTGCAGGGAGATACCGTTGGCAATCGCAGTCATACCGAATTcacgta < 1:112568/68‑3 (MQ=21) cAGGAAGCCACCGTGCAGGGAGATACCGTTAGCAATCGCGGTCATACCGAACTCGCGAACACCGTAGTg < 1:2166205/69‑1 (MQ=255) cAGGAAGCCACCGTGCAGGGAGATACCGTTAGCAATCGCGGTCATACCGAACTCGCGAACACCGTAGTg < 1:2841440/69‑1 (MQ=255) cAGGAAGCCACCGTGCAGGGAGATACCGTTAGCAATCGCGGTCATACCGAACTCGCGAACACCGTAGTg < 1:2107727/69‑1 (MQ=255) aGGAAGCCACCGTGCAGGGAGATACCGTTAGCAATCGCGGTCATACCGAACTCGCGAACACCGTAGTg < 1:268659/68‑1 (MQ=255) aaGCCACCGTGCAGGGAGATACCGTTAGCAATCGCGGTCATACCGAACTCGCGAACACCGTAGTGGa > 1:3345326/1‑67 (MQ=255) aaGCCACCGTGCAGGGAGATACCGTTAGCAATCGCGGTCATACCGAACTCGCGAACACCGTAGTGGa > 1:2206112/1‑67 (MQ=255) aaGCCACCGTGCAGGGAGATACCGTTAGCAATCGCGGTCATACCGAACTCGCGAACACCGTAGTGGa > 1:2862896/1‑67 (MQ=255) aaGCCACCGTGCAGGGAGATACCGTTAGCAATCGCGGTCATACCGAACTCGCGAACACCGTAGTGGa > 1:2257766/1‑67 (MQ=255) aaGCCACCGTGCAGGGAGATACCGTTAGCAATCGCGGTCATACCGAACTCGCGAACACCGTAGTGGa > 1:3429054/1‑67 (MQ=255) aaGCCACCGTGCAGGGAGATACCGTTAGCAATCGCGGTCATACCGAACTCGCGAACACCGTAGTGGa > 1:794175/1‑67 (MQ=255) aaGCCACCGTGCAGGGAGATACCGTTAGCAATCGCGGTCATACCGAACTCGCGAACACCGTAGTGGa > 1:1562746/1‑67 (MQ=255) cACCGTGCAGGGAGATACCGTTAGCAATCGCGGTCATACCGAACTcgc > 1:1782405/1‑48 (MQ=255) cACCGTGCAGGGAGATACCGTTAGCAATCGCGGTCATACCGAACTCGCGAACACCGTAGTGGATGTAGt > 1:12259/1‑69 (MQ=255) cACCGTGCAGGGAGATACCGTTAGCAATCGCGGTCATACCGAACTCGCGAACACCGTAGTGGATGTAGt > 1:72237/1‑69 (MQ=255) gagaTACCGTTAGCAATCGCGGTCATACCGAACTCGCGAACACCGTAGTGGATGTAGCTACCCgcagca < 1:2408366/69‑1 (MQ=255) aCCGTTAGCAATCGCGGTCATACCGAACTCGCGAACACCGTAGTGGATGTa < 1:3005394/51‑1 (MQ=255) aCCGTTAGCAATCGCGGTCATACCGAACTCGCGAACACCGTAGTGGATGTa < 1:1546590/51‑1 (MQ=255) aCCGTTAGCAATCGCGGTCATACCGAACTCGCGAACACCGTAGTGGATGTAGTTACCCGCAGCATCTTc > 1:2911363/1‑69 (MQ=255) aCCGTTAGCAATCGCGGTCATACCGAACTCGCGAACACCGTAGTGGATGTAGTTACCCGCAGCATCTTc > 1:2915444/1‑69 (MQ=255) aCCGTTAGCAATCGCGGTCATACCGAACTCGCGAACACCGTAGTGGATGTAGTTACCCGCAGCATCTTc > 1:2299892/1‑69 (MQ=255) ccGTTGGCAATCGCAGTCATACCGAATTCACGTACACCGTAGTGGATGTAGTTACCCGCAGCATCTTCg < 1:504729/69‑1 (MQ=255) ccGTTGGCAATCGCAGTCATACCGAATTCACGTACACCGTAGTGGATGTAGTTACCCGCAGCATCTTCg < 1:654429/69‑1 (MQ=255) ccGTTGGCAATCGCAGTCATACCGAATTCACGTACACCGTAGTGGATGTAGTTACCCGCAGCATCTTCg < 1:904909/69‑1 (MQ=255) ccGTTGGCAATCGCAGTCATACCGAATTCACGTACACCGTAGTGGATGTAGTTACCCGCAGCATCTTCg < 1:2645579/69‑1 (MQ=255) ccGTTGGCAATCGCAGTCATACCGAATTCACGTACACCGTAGTGGATGTAGTTACCCGCAGCATCTTCg < 1:1667559/69‑1 (MQ=255) ccGTTGGCAATCGCAGTCATACCGAATTCACGTACACCGTAGTGGATGTAGTTACCCGCAGCATCTTCg < 1:2213404/69‑1 (MQ=255) ccGTTGGCAATCGCAGTCATACCGAATTCACGTACACCGTAGTGGATGTAGTTACCCGCAGCATCTTCg < 1:1450941/69‑1 (MQ=255) tggCAATCGCAGTCATACCGAATTCACGTACACCGTAGTGGATGTAGTTACCCGCAGCATCTTCGTTGa < 1:2974079/67‑1 (MQ=255) tggCAATCGCAGTCATACCGAATTCACGTACACCGTAGTGGATGTAGTTACCCGCAGCATCTTCGTTGa < 1:2132596/67‑1 (MQ=255) tggCAATCGCAGTCATACCGAATTCACGTACACCGTAGTGGATGTAGTTACCCGCAGCATCTTCGTTGa < 1:2050818/67‑1 (MQ=255) tggCAATCGCAGTCATACCGAATTCACGTACACCGTAGTGGATGTAGTTACCCGCAGCATCTTCGTTGa < 1:3069125/67‑1 (MQ=255) tggCAATCGCAGTCATACCGAATTCACGTACACCGTAGTGGATGTAGTTACCCGCAGCATCTTCGTTGa < 1:3115126/67‑1 (MQ=255) tggCAATCGCAGTCATACCGAATTCACGTACACCGTAGTGGATGTAGTTACCCGCAGCATCTTCGTTGa < 1:138787/67‑1 (MQ=255) tggCAATCGCAGTCATACCGAATTCACGTACACCGTAGTGGATGTAGTTACCCGCAGCATCTTCGTTGa < 1:3161783/67‑1 (MQ=255) tggCAATCGCAGTCATACCGAATTCACGTACACCGTAGTGGATGTAGTTACCCGCAGCATCTTCGTTGa < 1:620063/67‑1 (MQ=255) tggCAATCGCAGTCATACCGAATTCACGTACACCGTAGTGGATGTAGTTACCCGCAGCATCTTCGTTGa < 1:3239033/67‑1 (MQ=255) tggCAATCGCAGTCATACCGAATTCACGTACACCGTAGTGGATGTAGTTACCCGCAGCATCTTCGTTGa < 1:150418/67‑1 (MQ=255) tggCAATCGCAGTCATACCGAATTCACGTACACCGTAGTGGATGTAGTTACCCGCAGCATCTTCGTTGa < 1:1529469/67‑1 (MQ=255) tggCAATCGCAGTCATACCGAATTCACGTACACCGTAGTGGATGTAGTTACCCGCAGCATCTTCGTTGa < 1:3306198/67‑1 (MQ=255) tggCAATCGCAGTCATACCGAATTCACGTACACCGTAGTGGATGTAGTTACCCGCAGCATCTTCGTTGa < 1:1580453/67‑1 (MQ=255) tggCAATCGCAGTCATACCGAATTCACGTACACCGTAGTGGATGTAGTTACCCGCAGCATCTTCGTTGa < 1:1934840/67‑1 (MQ=255) tggCAATCGCAGTCATACCGAATTCACGTACACCGTAGTGGATGTAGTTACCCGCAGCATCTTCGTTGa < 1:1607296/67‑1 (MQ=255) tggCAATCGCAGTCATACCGAATTCACGTACACCGTAGTGGATGTAGTTACCCGCAGCATCTTCGTTGa < 1:2933495/67‑1 (MQ=255) tggCAATCGCAGTCATACCGAATTCACGTACACCGTAGTGGATGTAGTTACCCGCAGCATCTTCGTTGa < 1:2434351/67‑1 (MQ=255) tggCAATCGCAGTCATACCGAATTCACGTACACCGTAGTGGATGTAGTTACCCGCAGCATCTTCGTTGa < 1:2502785/67‑1 (MQ=255) tggCAATCGCAGTCATACCGAATTCACGTACACCGTAGTGGATGTAGTTACCCGCAGCATCTTCGTTGa < 1:242233/67‑1 (MQ=255) tggCAATCGCAGTCATACCGAATTCACGTACACCGTAGTGGATGTAGTTACCCGCAGCATCTTCGTTGa < 1:2403978/67‑1 (MQ=255) tggCAATCGCAGTCATACCGAATTCACGTACACCGTAGTGGATGTAGTTACCCGCAGCATCTTCGTTGa < 1:2674111/67‑1 (MQ=255) tggCAATCGCAGTCATACCGAATTCACGTACACCGTAGTGGATGTAGTTACCCGCAGCATCTTCGTTGa < 1:889411/67‑1 (MQ=255) tggCAATCGCAGTCATACCGAATTCACGTACACCGTAGTGGATGTAGTTACCCGCAGCATCTTCGTTGa < 1:2276340/67‑1 (MQ=255) tggCAATCGCAGTCATACCGAATTCACGTACACCGTAGTGGATGTAGTTACCCGCAGCATCTTCGTTGa < 1:2861292/67‑1 (MQ=255) tggCAATCGCAGTCATACCGAATTCACGTACACCGTAGTGGATGTAGTTACCCGCAGCATCTTCGTTGa < 1:2204928/67‑1 (MQ=255) tggCAATCGCAGTCATACCGAATTCACGTACACCGTAGTGGATGTAGTTACCCGCAGCATCTTCGTTGa < 1:2925403/67‑1 (MQ=255) tAGCAATCGCGGTCATACCGAACTCGCGAACACCGTAGTg < 1:1269193/40‑1 (MQ=255) aGCAATCGCGGTCATACCGAACTCGCGAACACCGTAGTGGATGTAGTTACCCGCAGCATCTTCGTTGa > 1:1991688/1‑68 (MQ=255) gCAATCGCAGTCATACCGAATTCACGTACACCGTAGTGGATGTAGTTACCCGCAGCATCTTCGTTGa < 1:1743370/67‑1 (MQ=255) cgcgGTCATACCGAACTCGCGAACACCGTAGTGGATGt < 1:2782938/38‑1 (MQ=255) cgcgGTCATACCGAACTCGCGAACACCGTAGTGGATGt < 1:2288146/38‑1 (MQ=255) cgcgGTCATACCGAACTCGCGAACACCGTAGTGGATGt < 1:1380083/38‑1 (MQ=255) cgcgGTCATACCGAACTCGCGAACACCGTAGTGGATGt < 1:3401730/38‑1 (MQ=255) cgcgGTCATACCGAACTCGCGAACACCGTAGTGGATGt < 1:3435291/38‑1 (MQ=255) cgcgGTCATACCGAACTCGCGAACACCGTAGTGGATGt < 1:1920946/38‑1 (MQ=255) cgcgGTCATACCGAACTCGCGAACACCGTAGTGGATGt < 1:2936998/38‑1 (MQ=255) cgcgGTCATACCGAACTCGCGAACACCGTAGTGGATGt < 1:2426652/38‑1 (MQ=255) cgcgGTCATACCGAACTCGCGAACACCGTAGTGGATGTAGTTACCCGCAGCATCTTCGTTGATTGc > 1:112058/1‑66 (MQ=255) cgcgGTCATACCGAACTCGCGAACACCGTAGTGGATGTAGTTACCCGCAGCATCTTCGTTGATTGc > 1:285532/1‑66 (MQ=255) gcgGTCATACCGAACTCGCGAACACCGTAGTGGATGTAGTTACCCGCAGCATCTTCGTTGATTGCttt > 1:45477/1‑68 (MQ=255) gTCATACCGAATTCACGTACACCGTAGTGGATGTAGTTACCCGCAGCATCTTCGTTGATTGCTTTAGa > 1:1999312/1‑68 (MQ=255) gTCATACCGAATTCACGTACACCGTAGTGGATGTAGTTACCCGCAGCATCTTCGTTGATTGCTTTAGa > 1:3295299/1‑68 (MQ=255) gTCATACCGAATTCACGTACACCGTAGTGGATGTAGTTACCCGCAGCATCTTCGTTGATTGCTTTAGa > 1:5998/1‑68 (MQ=255) gTCATACCGAATTCACGTACACCGTAGTGGATGTAGTTACCCGCAGCATCTTCGTTGATTGCTTTAGa > 1:3217011/1‑68 (MQ=255) gTCATACCGAATTCACGTACACCGTAGTGGATGTAGTTACCCGCAGCATCTTCGTTGATTGCTTTAGa > 1:3130526/1‑68 (MQ=255) gTCATACCGAATTCACGTACACCGTAGTGGATGTAGTTACCCGCAGCATCTTCGTTGATTGCTTTAGa > 1:701577/1‑68 (MQ=255) gTCATACCGAATTCACGTACACCGTAGTGGATGTAGTTACCCGCAGCATCTTCGTTGATTGCTTTAGa > 1:3130017/1‑68 (MQ=255) gTCATACCGAATTCACGTACACCGTAGTGGATGTAGTTACCCGCAGCATCTTCGTTGATTGCTTTAGa > 1:2363493/1‑68 (MQ=255) gTCATACCGAATTCACGTACACCGTAGTGGATGTAGTTACCCGCAGCATCTTCGTTGATTGCTTTAGa > 1:2551578/1‑68 (MQ=255) tCATACCGAACTCGCGAGCACCGTAGTGGATGTAGTTACCCGCAGCATCTTCGTTGATTGCTTTAGAAc < 1:3313506/69‑1 (MQ=255) aCCGAACTCGCGAACACCGTAGTGGATGTAGTTACCCGCAGCATCTTCGTTGATTGCTTTAGAACCAg < 1:2193299/68‑1 (MQ=255) aCCGAACTCGCGAACACCGTAGTGGATGTAGTTACCCGCAGCATCTTCGTTGATTGCTTTAGAACCAg < 1:836238/68‑1 (MQ=255) | GGTGTACGGCAGGAAGCCACCGTGCAGGGAGATACCGTTAGCAATCGCGGTCATACCGAACTCGCGAACACCGTAGTGGATGTAGTTACCCGCAGCATCTTCGTTGATTGCTTTAGAACCAG > minE/1967337‑1967458 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |