| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,968,090 | T→C | 28.3% | A180A (GCA→GCG) | tktA ← | transketolase 1, thiamin‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,968,090 | 0 | T | C | 28.3% | 96.5 / 40.7 | 95 | A180A (GCA→GCG) | tktA | transketolase 1, thiamin‑binding |
| Reads supporting (aligned to +/- strand): ref base T (13/55); new base C (19/8); total (32/63) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.15e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCAT > minE/1968024‑1968155 | tGCGGTGTCGTCGGTAAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAACGc > 1:1052521/1‑69 (MQ=255)tGCGGTGTCGTCGGTAAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAACGc > 1:3144308/1‑69 (MQ=255)tGCGGTGTCGTCGGTAAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAACGc > 1:3086030/1‑69 (MQ=255)tGCGGTGTCGTCGGTAAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAACGc > 1:3080046/1‑69 (MQ=255)tGCGGTGTCGTCGGTAAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAACGc > 1:2935117/1‑69 (MQ=255)tGCGGTGTCGTCGGTAAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAACGc > 1:2786112/1‑69 (MQ=255)tGCGGTGTCGTCGGTAAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAACGc > 1:323185/1‑69 (MQ=255)tGCGGTGTCGTCGGTAAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAACGc > 1:2683537/1‑69 (MQ=255)tGCGGTGTCGTCGGTAAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAACGc > 1:2657359/1‑69 (MQ=255)tGCGGTGTCGTCGGTAAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAACGc > 1:368344/1‑69 (MQ=255)tGCGGTGTCGTCGGTAAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAACGc > 1:2522011/1‑69 (MQ=255)tGCGGTGTCGTCGGTAAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAACGc > 1:2368855/1‑69 (MQ=255)tGCGGTGTCGTCGGTAAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAACGc > 1:2338569/1‑69 (MQ=255)tGCGGTGTCGTCGGTAAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAACGc > 1:514886/1‑69 (MQ=255)tGCGGTGTCGTCGGTAAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAACGc > 1:2213777/1‑69 (MQ=255)tGCGGTGTCGTCGGTAAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAACGc > 1:1670750/1‑69 (MQ=255)tGCGGTGTCGTCGGTAAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAACGc > 1:1211944/1‑69 (MQ=255)tGCGGTGTCGTCGGTAAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAACGc > 1:1117582/1‑69 (MQ=255)tGCGGTGTCGTCGGTAAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAACGc > 1:1532532/1‑69 (MQ=255) gtgtCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAAt < 1:1252853/68‑1 (MQ=255) gtgtCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAAt < 1:2562002/68‑1 (MQ=255) gtgtCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAAt < 1:2550566/68‑1 (MQ=255) gtgtCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAAt < 1:875910/68‑1 (MQ=255) gtgtCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAAt < 1:2328728/68‑1 (MQ=255) gtgtCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAAt < 1:545346/68‑1 (MQ=255) tgtCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCa < 1:1675972/69‑1 (MQ=255) tgtCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCa < 1:815970/69‑1 (MQ=255) tgtCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCa < 1:2544501/69‑1 (MQ=255) tgtCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCa < 1:1994444/69‑1 (MQ=255) gtcgtcGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCa < 1:1650452/68‑1 (MQ=255) gtcgtcGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCa < 1:550554/68‑1 (MQ=255) tcgtcgGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAg > 1:3405183/1‑68 (MQ=255) tcgtcgGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAg > 1:2968659/1‑68 (MQ=255) aGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAACGCAATCAGTTTACCCAGCTTCa < 1:2108813/69‑1 (MQ=255) aGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAACGCAATCAGTTTACCCAGCTTCa < 1:1300182/69‑1 (MQ=255) aGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAACGCAATCAGTTTACCCAGCTTCa < 1:1840967/69‑1 (MQ=255) aGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAACGCAATCAGTTTACCCAGCTTCa < 1:1089070/69‑1 (MQ=255) aGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAACGCAATCAGTTTACCCAGCTTCa < 1:319913/69‑1 (MQ=255) aGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAACGCAATCAGTTTACCCAGCTTCa < 1:1446848/69‑1 (MQ=255) gCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAACGCAATCAGTTTACCCAGCTTCa < 1:1621640/68‑1 (MQ=255) gCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAACGCAATCAGTTTACCCAGCTTCa < 1:2149614/68‑1 (MQ=255) cTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCg < 1:3211731/69‑1 (MQ=255) cTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCg < 1:2780759/69‑1 (MQ=255) cTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCg < 1:1111208/69‑1 (MQ=255) cTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCg < 1:1017332/69‑1 (MQ=255) cTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCg < 1:3132050/69‑1 (MQ=255) cTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCg < 1:3142265/69‑1 (MQ=255) cTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCg < 1:3180022/69‑1 (MQ=255) cTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCg < 1:1589947/69‑1 (MQ=255) cTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCg < 1:886400/69‑1 (MQ=255) cTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCg < 1:2509555/69‑1 (MQ=255) cTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCg < 1:670607/69‑1 (MQ=255) cTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCg < 1:1409405/69‑1 (MQ=255) cTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCg < 1:659556/69‑1 (MQ=255) aCCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGc > 1:30978/1‑66 (MQ=255) aCCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGc > 1:3062588/1‑66 (MQ=255) aCCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGc > 1:3187446/1‑66 (MQ=255) gATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCa > 1:2227559/1‑69 (MQ=255) gATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCa > 1:2450667/1‑69 (MQ=255) aCCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGt > 1:1168227/1‑68 (MQ=255) gTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCGGAGAGCAAACTTCGTGGga < 1:288021/69‑1 (MQ=255) gTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTTGTGGga < 1:437163/69‑1 (MQ=255) gTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTTGga < 1:802474/69‑1 (MQ=255) gTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGga < 1:3416682/69‑1 (MQ=255) gTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGga < 1:401306/69‑1 (MQ=255) gTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGga < 1:718469/69‑1 (MQ=255) gTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGga < 1:757633/69‑1 (MQ=255) gTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGga < 1:945154/69‑1 (MQ=255) gTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGga < 1:3429885/69‑1 (MQ=255) gTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGga < 1:2022656/69‑1 (MQ=255) gTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGga < 1:1074990/69‑1 (MQ=255) gTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGga < 1:135/69‑1 (MQ=255) gTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGga < 1:1700254/69‑1 (MQ=255) gTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGga < 1:1702154/69‑1 (MQ=255) gTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGga < 1:1708131/69‑1 (MQ=255) gTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGga < 1:1732359/69‑1 (MQ=255) gTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGga < 1:1933503/69‑1 (MQ=255) gTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGga < 1:2265405/69‑1 (MQ=255) gTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGga < 1:2277292/69‑1 (MQ=255) gTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGga < 1:2457545/69‑1 (MQ=255) gTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGga < 1:2582058/69‑1 (MQ=255) gTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGga < 1:2582570/69‑1 (MQ=255) gTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGga < 1:2620425/69‑1 (MQ=255) gTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGga < 1:2721012/69‑1 (MQ=255) gTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGga < 1:2755591/69‑1 (MQ=255) gTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGga < 1:2864207/69‑1 (MQ=255) gTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGga < 1:2877343/69‑1 (MQ=255) gTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGga < 1:3020506/69‑1 (MQ=255) cATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATg < 1:741632/68‑1 (MQ=255) aGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTcc > 1:2355014/1‑68 (MQ=255) aGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTcc > 1:3176986/1‑68 (MQ=255) aGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTcc > 1:562251/1‑68 (MQ=255) aGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTcc > 1:1947811/1‑68 (MQ=255) aGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTcc > 1:1334560/1‑68 (MQ=255) gAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCcat < 1:601980/69‑1 (MQ=255) | TGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCAT > minE/1968024‑1968155 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |