| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,971,412 | T→C | 70.6% | A498A (GCA→GCG) | speA ← | biosynthetic arginine decarboxylase, PLP‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,971,412 | 0 | T | C | 70.6% | 59.8 / 24.7 | 61 | A498A (GCA→GCG) | speA | biosynthetic arginine decarboxylase, PLP‑binding |
| Reads supporting (aligned to +/- strand): ref base T (13/5); new base C (27/16); total (40/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.64e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.10e-01 | |||||||||||
CGACGTTCCGGCACTTGATCCAGCCCTTCCAGCGGCAGAACCGGGAACAACTGGTCGATCCCCCATGCGTCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTCCGCCA > minE/1971347‑1971464 | cgACGTTCCGGCACTTGATCCAGCCCTTCCAGCGGCAGAACCGGGAACAACTGGTCGATCCCCCATGCg < 1:2266865/69‑1 (MQ=255)cgACGTTCCGGCACTTGATCCAGCCCTTCCAGCGGCAGAACCGGGAACAACTGGTCGATCCCCCATGCg < 1:3013807/69‑1 (MQ=255)cgACGTTCCGGCACTTGATCCAGCCCTTCCAGCGGCAGAACCGGGAACAACTGGTCGATCCCCCATGCg < 1:3376070/69‑1 (MQ=255) ccGGCACTTGATCCAGCCCTTCCAGCGGCAGAACCGGGAACAACTGGTCGATCCCCCATGCGTCCGGCa > 1:828735/1‑69 (MQ=255) ccGGCACTTGATCCAGCCCTTCCAGCGGCAGAACCGGGAACAACTGGTCGATCCCCCATGCGTCCGGCa > 1:2697373/1‑69 (MQ=255) ccGGCACTTGATCCAGCCCTTCCAGCGGCAGAACCGGGAACAACTGGTCGATCCCCCATGCGTCCGGCa > 1:2266270/1‑69 (MQ=255) ccGGCACTTGATCCAGCCCTTCCAGCGGCAGAACCGGGAACAACTGGTCGATCCCCCATGCGTCCGGCa > 1:2856913/1‑69 (MQ=255) ccGGCACTTGATCCAGCCCTTCCAGCGGCAGAACCGGGAACAACTGGTCGATCCCCCATGCGTCCGGCa > 1:1882600/1‑69 (MQ=255) aCCGGGAACAACTGGTCGATCCCCCATGCGTCCGGCATCGTCTGGAACAGCGAGAAGTTGACGTACa > 1:1963343/1‑67 (MQ=255) aCCGGGAACAACTGGTCGATCCCCCATGCGTCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACAtt > 1:2470712/1‑69 (MQ=255) aCCGGGAACAACTGGTCGATCCCCCATGCGTCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACAtt > 1:1231988/1‑69 (MQ=255) aCCGGGAACAACTGGTCGATCCCCCATGCGTCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACAtt > 1:3311384/1‑69 (MQ=255) aCCGGGAACAACTGGTCGATCCCCCATGCGTCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACAt > 1:2405570/1‑68 (MQ=255) gggAACAACTGGTCGATCCCCCATGCGTCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTg < 1:1515656/69‑1 (MQ=255) gggAACAACTGGTCGATCCCCCATGCGTCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTg < 1:1542740/69‑1 (MQ=255) acTGGTCGATCCCCCATGCGTCCGGCATCGCCTGGAACAGCGAGAAGTTGACGTACATTTTGTCCGc > 1:891378/1‑67 (MQ=255) acTGGTCGATCCCCCATGCGTCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTCCGCCa > 1:702439/1‑69 (MQ=255) acTGGTCGATCCCACATGCGTCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTCCGCCa > 1:2612549/1‑69 (MQ=255) tggtggATCCCCCACGCATCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTc > 1:1712701/1‑62 (MQ=255) tGGTCGATCCCCCACGCATCCGTCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTc > 1:1697590/1‑62 (MQ=255) tGGTCGATCCCCCACGCATCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGt > 1:1516608/1‑61 (MQ=255) tGGTCGATCCCCCACGCATCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTc > 1:3017142/1‑62 (MQ=255) tGGTCGATCCCCCACGCATCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTc > 1:2990494/1‑62 (MQ=255) tGGTCGATCCCCCACGCATCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTc > 1:3120919/1‑62 (MQ=255) tGGTCGATCCCCCACGCATCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTc > 1:2904992/1‑62 (MQ=255) tGGTCGATCCCCCACGCATCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTc > 1:2717943/1‑62 (MQ=255) tGGTCGATCCCCCACGCATCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTc > 1:361814/1‑62 (MQ=255) tGGTCGATCCCCCACGCATCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTc > 1:440515/1‑62 (MQ=255) tGGTCGATCCCCCACGCATCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTc > 1:2713576/1‑62 (MQ=255) tGGTCGATCCCCCACGCATCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTc > 1:267348/1‑62 (MQ=255) tGGTCGATCCCCCACGCATCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTc > 1:468621/1‑62 (MQ=255) tGGTCGATCCCCCACGCATCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTc > 1:885330/1‑62 (MQ=255) tGGTCGATCCCCCACGCATCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTc > 1:896602/1‑62 (MQ=255) tGGTCGATCCCCCACGCATCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTc > 1:2381396/1‑62 (MQ=255) tGGTCGATCCCCCACGCATCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTc > 1:1640097/1‑62 (MQ=255) tGGTCGATCCCCCACGCATCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTc > 1:1523980/1‑62 (MQ=255) tGGTCGATCCCCCACGCATCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTc > 1:1479302/1‑62 (MQ=255) tGGTCGATCCCCCACGCATCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTc > 1:1934168/1‑62 (MQ=255) tGGTCGATCCCCCACGCATCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTc > 1:1461241/1‑62 (MQ=255) tGGTCGATCCCCCACGCATCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTc > 1:1940206/1‑62 (MQ=255) tGGTCGATCCCCCACGCATCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTc > 1:128282/1‑62 (MQ=255) tGGTCGATCCCCCACGCATCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTc > 1:20276/1‑62 (MQ=255) tGGTCGATCCCCCACGCATCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTc > 1:1034882/1‑62 (MQ=255) tGGTCGATCCCCCACGCATCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTc > 1:2671614/1‑62 (MQ=255) tGGTCCATCCCCCACGCATCCCGCATCCACTCGAACAGCGAGAAGTTGACGTACaat > 1:2841238/1‑55 (MQ=255) gTCTATCCCCCACGCATCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTCCGc < 1:1619242/63‑1 (MQ=255) gTCGATCCCCCACGCATCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTCCGc < 1:1306123/63‑1 (MQ=255) gTCGATCCCCCACGCATCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTCCGc < 1:42378/63‑1 (MQ=255) gTCGATCCCCCACGCATCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTCCGc < 1:416832/63‑1 (MQ=255) gTCGATCCCCCACGCATCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTCCGc < 1:1536393/63‑1 (MQ=255) gTCGATCCCCCACGCATCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTCCGc < 1:1027727/63‑1 (MQ=255) gTCGATCCCCCACGCATCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTCCGc < 1:3220777/63‑1 (MQ=255) gTCGATCCCCCACGCATCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTCCGc < 1:1772704/63‑1 (MQ=255) gTCGATCCCCCACGCATCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTCCGc < 1:1775285/63‑1 (MQ=255) gTCGATCCCCCACGCATCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTCCGc < 1:1776092/63‑1 (MQ=255) gTCGATCCCCCACGCATCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTCCGc < 1:2899256/63‑1 (MQ=255) gTCGATCCCCCACGCATCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTCCGc < 1:2853059/63‑1 (MQ=255) gTCGATCCCCCACGCATCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTCCGc < 1:2812811/63‑1 (MQ=255) gTCGATCCCCCACGCATCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTCCGc < 1:227226/63‑1 (MQ=255) gTCGATCCCCCACGCATCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTCCGc < 1:2278009/63‑1 (MQ=255) tCGATCCCCCACGCATCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTCCGc < 1:1873075/62‑1 (MQ=255) | CGACGTTCCGGCACTTGATCCAGCCCTTCCAGCGGCAGAACCGGGAACAACTGGTCGATCCCCCATGCGTCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTCCGCCA > minE/1971347‑1971464 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |