| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,971,856 | G→A | 20.7% | N350N (AAC→AAT) | speA ← | biosynthetic arginine decarboxylase, PLP‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,971,856 | 0 | G | A | 20.7% | 104.2 / 17.1 | 72 | N350N (AAC→AAT) | speA | biosynthetic arginine decarboxylase, PLP‑binding |
| Reads supporting (aligned to +/- strand): ref base G (38/19); new base A (2/13); total (40/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.86e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.69e-01 | |||||||||||
AGCACGGTGTGATGCGCAGTCACCGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTT > minE/1971791‑1971921 | aGCACGGTGTGATGCGCAGTCACCGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGttt > 1:2876539/1‑69 (MQ=255) gtgATGCGCAGTCACCGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTctca < 1:1465710/69‑1 (MQ=255) gtgATGCGCAGTCACCGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTcaca < 1:102579/69‑1 (MQ=255) gtgATGCGCAGTCACCGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTcaca < 1:533952/69‑1 (MQ=255) gtgATGCGCAGTCACCGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTcaca < 1:2165122/69‑1 (MQ=255) gtgATGCGCAGTCACCGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTcaca < 1:1431266/69‑1 (MQ=255) gtgATGCGCAGTCACCGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTcaca < 1:1426949/69‑1 (MQ=255) tgATGCGCAGTCACCGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTcaca < 1:2892257/68‑1 (MQ=255) aTGCGCAGTCACCGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGc > 1:1343666/1‑69 (MQ=255) aTGCGCAGTCACCGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGc > 1:731/1‑69 (MQ=255) aTGCGCAGTCACCGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGc > 1:489539/1‑69 (MQ=255) cgcAGTCACCGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACGCGCATc > 1:1004015/1‑69 (MQ=255) cgcAGTCACCGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATc > 1:2072249/1‑69 (MQ=255) cgcAGTCACCGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATc > 1:2006646/1‑69 (MQ=255) gTCACCGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCa < 1:1427251/69‑1 (MQ=255) tCACCGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCa < 1:2918839/68‑1 (MQ=255) acagCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATc > 1:2234186/4‑69 (MQ=255) aCCGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACCCGCATCGCCAAt > 1:2003347/1‑68 (MQ=255) aCCGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATc > 1:741371/1‑69 (MQ=255) aCCGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATc > 1:377413/1‑69 (MQ=255) aCCGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATc > 1:2641252/1‑69 (MQ=255) aCCGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATc > 1:3281380/1‑69 (MQ=255) aCCGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATc > 1:96170/1‑69 (MQ=255) aCCGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATc > 1:2829949/1‑69 (MQ=255) aCCGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATc > 1:3239238/1‑69 (MQ=255) aCCGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATc > 1:1515087/1‑69 (MQ=255) aCCGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATc > 1:3224101/1‑69 (MQ=255) cGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATc > 1:1873272/1‑67 (MQ=255) cGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCg > 1:2819737/1‑68 (MQ=255) cGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCg > 1:2417785/1‑68 (MQ=255) cGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCg > 1:2627913/1‑68 (MQ=255) cGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCg > 1:2500588/1‑68 (MQ=255) cGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCg > 1:2491250/1‑68 (MQ=255) cGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCg > 1:798861/1‑68 (MQ=255) cGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCg > 1:2165584/1‑68 (MQ=255) cGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCg > 1:1452869/1‑68 (MQ=255) cGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCg > 1:1760512/1‑68 (MQ=255) cGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCg > 1:1760810/1‑68 (MQ=255) cGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTGTTCTTCACACGCATCGCCAATc > 1:2533810/1‑67 (MQ=255) aCCCGATTCGGTGATTACCGTCGGATGCGGTAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGAt < 1:1122073/69‑1 (MQ=255) aCCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGAt < 1:738248/69‑1 (MQ=255) aCCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGAt < 1:1051191/69‑1 (MQ=255) aCCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGAt < 1:3266813/69‑1 (MQ=255) aCCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGAt < 1:3001552/69‑1 (MQ=255) aCCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGAt < 1:773605/69‑1 (MQ=255) aCCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGAt < 1:2399743/69‑1 (MQ=255) aCCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGAt < 1:2403925/69‑1 (MQ=255) aCCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCAAACGCATCGCCAATCGCCCAGAt < 1:3364/69‑1 (MQ=255) agATTCGGTGATTACCGTCGGATGTGGCAGACCATTTTCTTCACACGCATCACCAATCGCCCAgatgat < 1:2828910/68‑1 (MQ=255) agATTCGGTGATTACCGTCGGATGTGGCAGACCATTTTCTTCACACGCATCACCAATCGCCCAgatgat < 1:2128698/68‑1 (MQ=255) agATTCGGTGATTACCGTCGGATGTGGCAGACCATTTTCTTCACACGCATCACCAATCGCCCAgatgat < 1:1681679/68‑1 (MQ=255) agATTCGGTGATTACCGTCGGATGTGGCAGACCATTTTCTTCACACGCATCACCAATCGCCCAgatgat < 1:2947501/68‑1 (MQ=255) agATTCGGTGATTACCGTCGGATGTGGCAGACCATTTTCTTCACACGCATCACCAATCGCCCAgatgat < 1:2988097/68‑1 (MQ=255) agATTCGGTGATTACCGTCGGATGTGGCAGACCATTTTCTTCACACGCATCACCAATCGCCCAgatgat < 1:275733/68‑1 (MQ=255) agATTCGGTGATTACCGTCGGATGTGGCAGACCATTTTCTTCACACGCATCACCAATCGCCCAgatgat < 1:2720676/68‑1 (MQ=255) agATTCGGTGATTACCGTCGGATGTGGCAGACCATTTTCTTCACACGCATCACCAATCGCCCAgatgat < 1:3168578/68‑1 (MQ=255) agATTCGGTGATTACCGTCGGATGTGGCAGACCATTTTCTTCACACGCATCACCAATCGCCCAgatgat < 1:1075123/68‑1 (MQ=255) agATTCGGTGATTACCGTCGGATGTGGCAGACCATTTTCTTCACACGCATCACCAATCGCCCAgatgat < 1:2542734/68‑1 (MQ=255) agATTCGGTGATTACCGTCGGATGTGGCAGACCATTTTCTTCACACGCATCACCAATCGCCCAgatgat < 1:361707/68‑1 (MQ=255) gATTCGGTGATTACCGTCGGATGTGGCAGACCATTTTCTTCACACGCATCACCAATCGCCCAgatgat < 1:982124/68‑1 (MQ=255) gATTCGGTGATTACCGTCGGATGTGGCAGACCATTTTCTTCACACGCATCACCAATCGCCCAgatgat < 1:2637126/68‑1 (MQ=255) tCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAAtgttg > 1:1436253/1‑69 (MQ=255) tCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAAtgtt > 1:3333425/1‑68 (MQ=255) tACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCg > 1:1304559/1‑67 (MQ=255) tACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGt > 1:3109256/1‑68 (MQ=255) tACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGt > 1:257588/1‑68 (MQ=255) tACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGt > 1:190338/1‑68 (MQ=255) tACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGt > 1:1391267/1‑68 (MQ=255) tACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTa > 1:2424084/1‑69 (MQ=255) ggCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTAtt < 1:3362527/58‑1 (MQ=255) aCCATTTTCTTCACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGtt > 1:1743462/1‑69 (MQ=255) aCCATTTTCTTCACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGtt > 1:840556/1‑69 (MQ=255) | AGCACGGTGTGATGCGCAGTCACCGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTT > minE/1971791‑1971921 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |