| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,971,874 | G→A | 47.2% | G344G (GGC→GGT) | speA ← | biosynthetic arginine decarboxylase, PLP‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,971,874 | 0 | G | A | 47.2% | 8.1 / 77.8 | 91 | G344G (GGC→GGT) | speA | biosynthetic arginine decarboxylase, PLP‑binding |
| Reads supporting (aligned to +/- strand): ref base G (34/14); new base A (2/41); total (36/55) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.67e-11 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTCACCGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGT > minE/1971809‑1971932 | gTCACCGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCa < 1:1427251/69‑1 (MQ=255) tCACCGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCa < 1:2918839/68‑1 (MQ=255) acagCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATc > 1:2234186/4‑69 (MQ=255) aCCGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACCCGCATCGCCAAt > 1:2003347/1‑68 (MQ=255) aCCGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATc > 1:3281380/1‑69 (MQ=255) aCCGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATc > 1:2829949/1‑69 (MQ=255) aCCGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATc > 1:3224101/1‑69 (MQ=255) aCCGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATc > 1:3239238/1‑69 (MQ=255) aCCGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATc > 1:1515087/1‑69 (MQ=255) aCCGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATc > 1:96170/1‑69 (MQ=255) aCCGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATc > 1:741371/1‑69 (MQ=255) aCCGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATc > 1:2641252/1‑69 (MQ=255) aCCGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATc > 1:377413/1‑69 (MQ=255) cGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATc > 1:1873272/1‑67 (MQ=255) cGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCg > 1:1760810/1‑68 (MQ=255) cGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCg > 1:2627913/1‑68 (MQ=255) cGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCg > 1:2165584/1‑68 (MQ=255) cGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCg > 1:2417785/1‑68 (MQ=255) cGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCg > 1:798861/1‑68 (MQ=255) cGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCg > 1:1760512/1‑68 (MQ=255) cGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCg > 1:2491250/1‑68 (MQ=255) cGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCg > 1:2500588/1‑68 (MQ=255) cGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCg > 1:2819737/1‑68 (MQ=255) cGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCg > 1:1452869/1‑68 (MQ=255) cGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTGTTCTTCACACGCATCGCCAATc > 1:2533810/1‑67 (MQ=255) aCCCGATTCGGTGATTACCGTCGGATGCGGTAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGAt < 1:1122073/69‑1 (MQ=255) aCCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGAt < 1:1051191/69‑1 (MQ=255) aCCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGAt < 1:2403925/69‑1 (MQ=255) aCCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGAt < 1:2399743/69‑1 (MQ=255) aCCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGAt < 1:3001552/69‑1 (MQ=255) aCCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGAt < 1:3266813/69‑1 (MQ=255) aCCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGAt < 1:738248/69‑1 (MQ=255) aCCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGAt < 1:773605/69‑1 (MQ=255) aCCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCAAACGCATCGCCAATCGCCCAGAt < 1:3364/69‑1 (MQ=255) agATTCGGTGATTACCGTCGGATGTGGCAGACCATTTTCTTCACACGCATCACCAATCGCCCAgatgat < 1:2947501/68‑1 (MQ=255) agATTCGGTGATTACCGTCGGATGTGGCAGACCATTTTCTTCACACGCATCACCAATCGCCCAgatgat < 1:2988097/68‑1 (MQ=255) agATTCGGTGATTACCGTCGGATGTGGCAGACCATTTTCTTCACACGCATCACCAATCGCCCAgatgat < 1:3168578/68‑1 (MQ=255) agATTCGGTGATTACCGTCGGATGTGGCAGACCATTTTCTTCACACGCATCACCAATCGCCCAgatgat < 1:361707/68‑1 (MQ=255) agATTCGGTGATTACCGTCGGATGTGGCAGACCATTTTCTTCACACGCATCACCAATCGCCCAgatgat < 1:2828910/68‑1 (MQ=255) agATTCGGTGATTACCGTCGGATGTGGCAGACCATTTTCTTCACACGCATCACCAATCGCCCAgatgat < 1:275733/68‑1 (MQ=255) agATTCGGTGATTACCGTCGGATGTGGCAGACCATTTTCTTCACACGCATCACCAATCGCCCAgatgat < 1:2720676/68‑1 (MQ=255) agATTCGGTGATTACCGTCGGATGTGGCAGACCATTTTCTTCACACGCATCACCAATCGCCCAgatgat < 1:1075123/68‑1 (MQ=255) agATTCGGTGATTACCGTCGGATGTGGCAGACCATTTTCTTCACACGCATCACCAATCGCCCAgatgat < 1:2128698/68‑1 (MQ=255) agATTCGGTGATTACCGTCGGATGTGGCAGACCATTTTCTTCACACGCATCACCAATCGCCCAgatgat < 1:2542734/68‑1 (MQ=255) agATTCGGTGATTACCGTCGGATGTGGCAGACCATTTTCTTCACACGCATCACCAATCGCCCAgatgat < 1:1681679/68‑1 (MQ=255) gATTCGGTGATTACCGTCGGATGTGGCAGACCATTTTCTTCACACGCATCACCAATCGCCCAgatgat < 1:2637126/68‑1 (MQ=255) gATTCGGTGATTACCGTCGGATGTGGCAGACCATTTTCTTCACACGCATCACCAATCGCCCAgatgat < 1:982124/68‑1 (MQ=255) tCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAAtgttg > 1:1436253/1‑69 (MQ=255) tCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAAtgtt > 1:3333425/1‑68 (MQ=255) tACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCg > 1:1304559/1‑67 (MQ=255) tACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGt > 1:257588/1‑68 (MQ=255) tACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGt > 1:1391267/1‑68 (MQ=255) tACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGt > 1:190338/1‑68 (MQ=255) tACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGt > 1:3109256/1‑68 (MQ=255) tACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTa > 1:2424084/1‑69 (MQ=255) ggCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTAtt < 1:3362527/58‑1 (MQ=255) aCCATTTTCTTCACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGtt > 1:1743462/1‑69 (MQ=255) aCCATTTTCTTCACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGtt > 1:840556/1‑69 (MQ=255) tttCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCg < 1:653378/69‑1 (MQ=255) tttCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCg < 1:338206/69‑1 (MQ=255) tcttcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCGTTGAGGCCGTAGTTTAc < 1:3272108/65‑1 (MQ=255) tcttcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:14841/65‑1 (MQ=255) tcttcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:3380881/65‑1 (MQ=255) tcttcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:592336/65‑1 (MQ=255) tcttcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:688253/65‑1 (MQ=255) tcttcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:1619740/65‑1 (MQ=255) tcttcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:1350291/65‑1 (MQ=255) tcttcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:799789/65‑1 (MQ=255) tcttcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:871705/65‑1 (MQ=255) tcttcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:1125042/65‑1 (MQ=255) tcttcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:2616220/65‑1 (MQ=255) tcttcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:1960460/65‑1 (MQ=255) tcttcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:2702946/65‑1 (MQ=255) tcttcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:2577680/65‑1 (MQ=255) tcttcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:2753399/65‑1 (MQ=255) tcttcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:2758550/65‑1 (MQ=255) tcttcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:2838465/65‑1 (MQ=255) tcttcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:2873702/65‑1 (MQ=255) tcttcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:1656996/65‑1 (MQ=255) tcttcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:1957408/65‑1 (MQ=255) tcttcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:301720/65‑1 (MQ=255) tcttcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:3052200/65‑1 (MQ=255) tcttcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:3093049/65‑1 (MQ=255) tcttcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:3194919/65‑1 (MQ=255) tcttcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:1744018/65‑1 (MQ=255) cttcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:2971062/64‑1 (MQ=255) tcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:319876/62‑1 (MQ=255) cacaCGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGATCAGt > 1:2756158/1‑69 (MQ=255) cacaCGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGt > 1:1919032/1‑69 (MQ=255) cacaCGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGt > 1:255302/1‑69 (MQ=255) aTCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:1401356/54‑1 (MQ=255) | GTCACCGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGT > minE/1971809‑1971932 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |