| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,971,889 | A→G | 73.7% | I339I (ATT→ATC) | speA ← | biosynthetic arginine decarboxylase, PLP‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,971,889 | 0 | A | G | 73.7% | 78.9 / 23.4 | 68 | I339I (ATT→ATC) | speA | biosynthetic arginine decarboxylase, PLP‑binding |
| Reads supporting (aligned to +/- strand): ref base A (13/5); new base G (21/29); total (34/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.26e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATA > minE/1971823‑1971957 | agATTCGGTGATTACCGTCGGATGTGGCAGACCATTTTCTTCACACGCATCACCAATCGCCCAgatgat < 1:275733/68‑1 (MQ=255)agATTCGGTGATTACCGTCGGATGTGGCAGACCATTTTCTTCACACGCATCACCAATCGCCCAgatgat < 1:2720676/68‑1 (MQ=255)agATTCGGTGATTACCGTCGGATGTGGCAGACCATTTTCTTCACACGCATCACCAATCGCCCAgatgat < 1:1075123/68‑1 (MQ=255)agATTCGGTGATTACCGTCGGATGTGGCAGACCATTTTCTTCACACGCATCACCAATCGCCCAgatgat < 1:2828910/68‑1 (MQ=255)agATTCGGTGATTACCGTCGGATGTGGCAGACCATTTTCTTCACACGCATCACCAATCGCCCAgatgat < 1:2947501/68‑1 (MQ=255)agATTCGGTGATTACCGTCGGATGTGGCAGACCATTTTCTTCACACGCATCACCAATCGCCCAgatgat < 1:2542734/68‑1 (MQ=255)agATTCGGTGATTACCGTCGGATGTGGCAGACCATTTTCTTCACACGCATCACCAATCGCCCAgatgat < 1:2988097/68‑1 (MQ=255)agATTCGGTGATTACCGTCGGATGTGGCAGACCATTTTCTTCACACGCATCACCAATCGCCCAgatgat < 1:2128698/68‑1 (MQ=255)agATTCGGTGATTACCGTCGGATGTGGCAGACCATTTTCTTCACACGCATCACCAATCGCCCAgatgat < 1:3168578/68‑1 (MQ=255)agATTCGGTGATTACCGTCGGATGTGGCAGACCATTTTCTTCACACGCATCACCAATCGCCCAgatgat < 1:361707/68‑1 (MQ=255)agATTCGGTGATTACCGTCGGATGTGGCAGACCATTTTCTTCACACGCATCACCAATCGCCCAgatgat < 1:1681679/68‑1 (MQ=255) gATTCGGTGATTACCGTCGGATGTGGCAGACCATTTTCTTCACACGCATCACCAATCGCCCAgatgat < 1:982124/68‑1 (MQ=255) gATTCGGTGATTACCGTCGGATGTGGCAGACCATTTTCTTCACACGCATCACCAATCGCCCAgatgat < 1:2637126/68‑1 (MQ=255) tCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAAtgttg > 1:1436253/1‑69 (MQ=255) tCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAAtgtt > 1:3333425/1‑68 (MQ=255) tACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCg > 1:1304559/1‑67 (MQ=255) tACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGt > 1:3109256/1‑68 (MQ=255) tACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGt > 1:257588/1‑68 (MQ=255) tACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGt > 1:1391267/1‑68 (MQ=255) tACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGt > 1:190338/1‑68 (MQ=255) tACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTa > 1:2424084/1‑69 (MQ=255) ggCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTAtt < 1:3362527/58‑1 (MQ=255) aCCATTTTCTTCACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGtt > 1:840556/1‑69 (MQ=255) aCCATTTTCTTCACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGtt > 1:1743462/1‑69 (MQ=255) tttCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCg < 1:653378/69‑1 (MQ=255) tttCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCg < 1:338206/69‑1 (MQ=255) tcttcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCGTTGAGGCCGTAGTTTAc < 1:3272108/65‑1 (MQ=255) tcttcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:871705/65‑1 (MQ=255) tcttcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:2758550/65‑1 (MQ=255) tcttcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:799789/65‑1 (MQ=255) tcttcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:2838465/65‑1 (MQ=255) tcttcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:2873702/65‑1 (MQ=255) tcttcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:688253/65‑1 (MQ=255) tcttcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:301720/65‑1 (MQ=255) tcttcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:3052200/65‑1 (MQ=255) tcttcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:3093049/65‑1 (MQ=255) tcttcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:592336/65‑1 (MQ=255) tcttcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:3194919/65‑1 (MQ=255) tcttcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:3380881/65‑1 (MQ=255) tcttcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:2753399/65‑1 (MQ=255) tcttcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:1125042/65‑1 (MQ=255) tcttcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:1350291/65‑1 (MQ=255) tcttcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:14841/65‑1 (MQ=255) tcttcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:1619740/65‑1 (MQ=255) tcttcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:1656996/65‑1 (MQ=255) tcttcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:1744018/65‑1 (MQ=255) tcttcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:1957408/65‑1 (MQ=255) tcttcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:1960460/65‑1 (MQ=255) tcttcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:2702946/65‑1 (MQ=255) tcttcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:2616220/65‑1 (MQ=255) tcttcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:2577680/65‑1 (MQ=255) cttcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:2971062/64‑1 (MQ=255) tcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:319876/62‑1 (MQ=255) cacaCGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGATCAGt > 1:2756158/1‑69 (MQ=255) cacaCGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGt > 1:255302/1‑69 (MQ=255) cacaCGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGt > 1:1919032/1‑69 (MQ=255) aTCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:1401356/54‑1 (MQ=255) accAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:2936259/50‑1 (MQ=255) ccAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTACGGAGCAGTCAGACTGAGaa > 1:3113155/1‑69 (MQ=255) ccAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTACGGAGCAGTCAGACTGAGaa > 1:902332/1‑69 (MQ=255) ccAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTACGGAGCAGTCAGACTGAGaa > 1:1196652/1‑69 (MQ=255) ccAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTACGGAGCAGTCAGACTGAGaa > 1:1229318/1‑69 (MQ=255) ccAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTACGGAGCAGTCAGACTGAGaa > 1:3010784/1‑69 (MQ=255) ccAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTACGGAGCAGTCAGACTGAGaa > 1:782268/1‑69 (MQ=255) ccAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTACGGAGCAGTCAGACTGAGaa > 1:68579/1‑69 (MQ=255) ccAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTACGGAGCAGTCAGACTGAGaa > 1:2784022/1‑69 (MQ=255) ccAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTACGGAGCAGTCAGACTGAGaa > 1:529345/1‑69 (MQ=255) ccAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTACGGAGCAGTCAGACTGAGaa > 1:473729/1‑69 (MQ=255) ccAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTACGGAGCAGTCAGACTGAGaa > 1:389537/1‑69 (MQ=255) ccAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTACGGAGCAGTCAGACTGAGaa > 1:380015/1‑69 (MQ=255) ccAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTACGGAGCAGTCAGACTGAGaa > 1:370606/1‑69 (MQ=255) ccAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTACGGAGCAGTCAGACTGAGaa > 1:2639000/1‑69 (MQ=255) ccAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTACGGAGCAGTCAGACTGAGaa > 1:3423026/1‑69 (MQ=255) ccAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTACGGAGCAGTCAGACTGAGaa > 1:1695219/1‑69 (MQ=255) ccAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTACGGAGCAGTCAGACTGAGaa > 1:3331415/1‑69 (MQ=255) ccAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTACGGAGCAGTCAGACTGAGaa > 1:1923788/1‑69 (MQ=255) ccAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTACGGAGCAGTCAGACTGAGaa > 1:230099/1‑69 (MQ=255) aaTCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACg < 1:1523618/69‑1 (MQ=255) aaTCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACg < 1:2124176/69‑1 (MQ=255) cGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGt > 1:2364673/1‑69 (MQ=255) cGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGt > 1:3208619/1‑69 (MQ=255) aaTGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATa < 1:2483269/69‑1 (MQ=255) aaTGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATa < 1:2487157/69‑1 (MQ=255) | CGATTCGGTGATTACCGTCGGATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATA > minE/1971823‑1971957 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |