| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,971,922 | C→T | 52.2% | V328V (GTG→GTA) | speA ← | biosynthetic arginine decarboxylase, PLP‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,971,922 | 0 | C | T | 52.2% | 1.6 / 82.1 | 92 | V328V (GTG→GTA) | speA | biosynthetic arginine decarboxylase, PLP‑binding |
| Reads supporting (aligned to +/- strand): ref base C (15/29); new base T (19/29); total (34/58) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.68e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCA > minE/1971858‑1971990 | tttCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCg < 1:653378/69‑1 (MQ=255)tttCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCg < 1:338206/69‑1 (MQ=255) tcttcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCGTTGAGGCCGTAGTTTAc < 1:3272108/65‑1 (MQ=255) tcttcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:3093049/65‑1 (MQ=255) tcttcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:2577680/65‑1 (MQ=255) tcttcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:2616220/65‑1 (MQ=255) tcttcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:2702946/65‑1 (MQ=255) tcttcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:2753399/65‑1 (MQ=255) tcttcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:2758550/65‑1 (MQ=255) tcttcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:1960460/65‑1 (MQ=255) tcttcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:1957408/65‑1 (MQ=255) tcttcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:2838465/65‑1 (MQ=255) tcttcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:2873702/65‑1 (MQ=255) tcttcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:1744018/65‑1 (MQ=255) tcttcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:301720/65‑1 (MQ=255) tcttcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:3052200/65‑1 (MQ=255) tcttcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:1656996/65‑1 (MQ=255) tcttcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:1619740/65‑1 (MQ=255) tcttcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:3194919/65‑1 (MQ=255) tcttcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:14841/65‑1 (MQ=255) tcttcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:3380881/65‑1 (MQ=255) tcttcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:1350291/65‑1 (MQ=255) tcttcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:592336/65‑1 (MQ=255) tcttcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:688253/65‑1 (MQ=255) tcttcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:799789/65‑1 (MQ=255) tcttcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:871705/65‑1 (MQ=255) tcttcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:1125042/65‑1 (MQ=255) cttcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:2971062/64‑1 (MQ=255) tcACACGCATCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:319876/62‑1 (MQ=255) cacaCGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGATCAGt > 1:2756158/1‑69 (MQ=255) cacaCGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGt > 1:1919032/1‑69 (MQ=255) cacaCGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGt > 1:255302/1‑69 (MQ=255) aTCACCAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:1401356/54‑1 (MQ=255) accAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTAc < 1:2936259/50‑1 (MQ=255) ccAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTACGGAGCAGTCAGACTGAGaa > 1:1923788/1‑69 (MQ=255) ccAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTACGGAGCAGTCAGACTGAGaa > 1:230099/1‑69 (MQ=255) ccAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTACGGAGCAGTCAGACTGAGaa > 1:3010784/1‑69 (MQ=255) ccAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTACGGAGCAGTCAGACTGAGaa > 1:1695219/1‑69 (MQ=255) ccAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTACGGAGCAGTCAGACTGAGaa > 1:3113155/1‑69 (MQ=255) ccAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTACGGAGCAGTCAGACTGAGaa > 1:3331415/1‑69 (MQ=255) ccAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTACGGAGCAGTCAGACTGAGaa > 1:3423026/1‑69 (MQ=255) ccAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTACGGAGCAGTCAGACTGAGaa > 1:370606/1‑69 (MQ=255) ccAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTACGGAGCAGTCAGACTGAGaa > 1:380015/1‑69 (MQ=255) ccAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTACGGAGCAGTCAGACTGAGaa > 1:389537/1‑69 (MQ=255) ccAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTACGGAGCAGTCAGACTGAGaa > 1:473729/1‑69 (MQ=255) ccAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTACGGAGCAGTCAGACTGAGaa > 1:529345/1‑69 (MQ=255) ccAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTACGGAGCAGTCAGACTGAGaa > 1:1229318/1‑69 (MQ=255) ccAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTACGGAGCAGTCAGACTGAGaa > 1:68579/1‑69 (MQ=255) ccAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTACGGAGCAGTCAGACTGAGaa > 1:782268/1‑69 (MQ=255) ccAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTACGGAGCAGTCAGACTGAGaa > 1:1196652/1‑69 (MQ=255) ccAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTACGGAGCAGTCAGACTGAGaa > 1:902332/1‑69 (MQ=255) ccAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTACGGAGCAGTCAGACTGAGaa > 1:2784022/1‑69 (MQ=255) ccAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTACGGAGCAGTCAGACTGAGaa > 1:2639000/1‑69 (MQ=255) aaTCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACg < 1:2124176/69‑1 (MQ=255) aaTCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACg < 1:1523618/69‑1 (MQ=255) cGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGt > 1:2364673/1‑69 (MQ=255) cGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGt > 1:3208619/1‑69 (MQ=255) aaTGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATa < 1:2483269/69‑1 (MQ=255) aaTGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATa < 1:2487157/69‑1 (MQ=255) aTGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATaa < 1:283719/69‑1 (MQ=255) aTGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATaa < 1:853436/69‑1 (MQ=255) aTGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATaa < 1:1300562/69‑1 (MQ=255) aTGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATaa < 1:420101/69‑1 (MQ=255) aTGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATaa < 1:3107842/69‑1 (MQ=255) aTGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATaa < 1:2939213/69‑1 (MQ=255) aTGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATaa < 1:2054038/69‑1 (MQ=255) aTGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATa < 1:419807/68‑1 (MQ=255) aTGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATa < 1:2373790/68‑1 (MQ=255) gttgttGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATAATc > 1:622461/1‑69 (MQ=255) gttgttGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATAATc > 1:2524258/1‑69 (MQ=255) gttgttGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATAATc > 1:2045850/1‑69 (MQ=255) gttgttGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATAATc > 1:967167/1‑69 (MQ=255) ttgttgGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCTGACTGCTAACGAGTACCTTCATaa < 1:2907334/66‑1 (MQ=255) gttgGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATAATCCa < 1:2688682/68‑1 (MQ=255) gttgGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATAATCCa < 1:3430517/68‑1 (MQ=255) gttgGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATAATCCa < 1:3241055/68‑1 (MQ=255) gttgGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATAATCCa < 1:403994/68‑1 (MQ=255) aTTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATAATCCACGCCCAGAc < 1:1679931/69‑1 (MQ=255) gAGGCCGTAGTTCACCGAACAGTCGGACTGCTAACGAGTACCTTCATAATCCACGCCCAGAccgccgcc < 1:217942/69‑1 (MQ=255) gAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATAATCCACGCCCAGAccgccgcc < 1:2430607/69‑1 (MQ=255) gAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATAATCCACGCCCAGAccgccgcc < 1:4162/69‑1 (MQ=255) gAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATAATCCACGCCCAGAccgccgcc < 1:121414/69‑1 (MQ=255) gAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATAATCCACGCCCAGAccgccgcc < 1:2111334/69‑1 (MQ=255) gAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATAATCCACGCCCAGAccgccgcc < 1:3354413/69‑1 (MQ=255) gAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATAATCCACGCCCAGAccgccgcc < 1:1514907/69‑1 (MQ=255) gAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATAATCCACGCCCAGAccgccgcc < 1:2667815/69‑1 (MQ=255) gTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGa > 1:2475707/1‑68 (MQ=255) gTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGa > 1:1087814/1‑68 (MQ=255) gTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGa > 1:1183401/1‑68 (MQ=255) gTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGa > 1:2278498/1‑68 (MQ=255) gTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGa > 1:927662/1‑68 (MQ=255) gTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATAATCCACGCCCAGACAGCCGCCGACGTCGa > 1:1796948/1‑68 (MQ=255) cACCGAACAGTCGGACTGCGAACGAGTAGCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCa > 1:1649022/1‑69 (MQ=255) cACCGAACAGTCGGACTGCGAACGAGTACCTTCATAATCCACGCCCAGAccgccgcc > 1:2519722/1‑57 (MQ=255) cACCGAACAGTCGGACTGCGAACGAGTACCTTCATAATCCACGCCCAGAccgccgcc > 1:515480/1‑57 (MQ=255) cACCGAACAGTCGGACTGCGAACGAGTACCTTCATAATCCACGCCCAGAccgccgcc > 1:1460357/1‑57 (MQ=255) cACCGAACAGTCGGACTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCa > 1:2626575/1‑69 (MQ=255) cACCGAACAGGCGGACTGCGAACGAGTACCTTCATAATCCACGCCCAGAccgccgcc < 1:1597310/57‑1 (MQ=255) | TTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCA > minE/1971858‑1971990 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |