| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,971,940 | C→A | 39.5% | S322S (TCG→TCT) | speA ← | biosynthetic arginine decarboxylase, PLP‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,971,940 | 0 | C | A | 39.5% | 67.1 / 76.2 | 91 | S322S (TCG→TCT) | speA | biosynthetic arginine decarboxylase, PLP‑binding |
| Reads supporting (aligned to +/- strand): ref base C (17/38); new base A (19/17); total (36/55) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.90e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.79e-01 | |||||||||||
CCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCC > minE/1971875‑1972005 | ccAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTACGGAGCAGTCAGACTGAGaa > 1:902332/1‑69 (MQ=255)ccAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTACGGAGCAGTCAGACTGAGaa > 1:2639000/1‑69 (MQ=255)ccAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTACGGAGCAGTCAGACTGAGaa > 1:230099/1‑69 (MQ=255)ccAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTACGGAGCAGTCAGACTGAGaa > 1:2784022/1‑69 (MQ=255)ccAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTACGGAGCAGTCAGACTGAGaa > 1:3010784/1‑69 (MQ=255)ccAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTACGGAGCAGTCAGACTGAGaa > 1:1923788/1‑69 (MQ=255)ccAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTACGGAGCAGTCAGACTGAGaa > 1:3113155/1‑69 (MQ=255)ccAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTACGGAGCAGTCAGACTGAGaa > 1:3331415/1‑69 (MQ=255)ccAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTACGGAGCAGTCAGACTGAGaa > 1:1695219/1‑69 (MQ=255)ccAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTACGGAGCAGTCAGACTGAGaa > 1:3423026/1‑69 (MQ=255)ccAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTACGGAGCAGTCAGACTGAGaa > 1:370606/1‑69 (MQ=255)ccAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTACGGAGCAGTCAGACTGAGaa > 1:380015/1‑69 (MQ=255)ccAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTACGGAGCAGTCAGACTGAGaa > 1:389537/1‑69 (MQ=255)ccAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTACGGAGCAGTCAGACTGAGaa > 1:1196652/1‑69 (MQ=255)ccAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTACGGAGCAGTCAGACTGAGaa > 1:473729/1‑69 (MQ=255)ccAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTACGGAGCAGTCAGACTGAGaa > 1:529345/1‑69 (MQ=255)ccAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTACGGAGCAGTCAGACTGAGaa > 1:1229318/1‑69 (MQ=255)ccAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTACGGAGCAGTCAGACTGAGaa > 1:68579/1‑69 (MQ=255)ccAATCGCCCAGATGATGTTGTTGGCGTATTCATTGAGGCCGTAGTTTACGGAGCAGTCAGACTGAGaa > 1:782268/1‑69 (MQ=255) aaTCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACg < 1:1523618/69‑1 (MQ=255) aaTCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACg < 1:2124176/69‑1 (MQ=255) cGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGt > 1:3208619/1‑69 (MQ=255) cGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGt > 1:2364673/1‑69 (MQ=255) aaTGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATa < 1:2487157/69‑1 (MQ=255) aaTGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATa < 1:2483269/69‑1 (MQ=255) aTGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATaa < 1:1300562/69‑1 (MQ=255) aTGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATaa < 1:3107842/69‑1 (MQ=255) aTGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATaa < 1:2054038/69‑1 (MQ=255) aTGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATaa < 1:420101/69‑1 (MQ=255) aTGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATaa < 1:2939213/69‑1 (MQ=255) aTGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATaa < 1:283719/69‑1 (MQ=255) aTGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATaa < 1:853436/69‑1 (MQ=255) aTGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATa < 1:419807/68‑1 (MQ=255) aTGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATa < 1:2373790/68‑1 (MQ=255) gttgttGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATAATc > 1:622461/1‑69 (MQ=255) gttgttGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATAATc > 1:967167/1‑69 (MQ=255) gttgttGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATAATc > 1:2524258/1‑69 (MQ=255) gttgttGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATAATc > 1:2045850/1‑69 (MQ=255) ttgttgGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCTGACTGCTAACGAGTACCTTCATaa < 1:2907334/66‑1 (MQ=255) gttgGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATAATCCa < 1:3241055/68‑1 (MQ=255) gttgGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATAATCCa < 1:403994/68‑1 (MQ=255) gttgGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATAATCCa < 1:3430517/68‑1 (MQ=255) gttgGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATAATCCa < 1:2688682/68‑1 (MQ=255) aTTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATAATCCACGCCCAGAc < 1:1679931/69‑1 (MQ=255) gAGGCCGTAGTTCACCGAACAGTCGGACTGCTAACGAGTACCTTCATAATCCACGCCCAGAccgccgcc < 1:217942/69‑1 (MQ=255) gAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATAATCCACGCCCAGAccgccgcc < 1:2667815/69‑1 (MQ=255) gAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATAATCCACGCCCAGAccgccgcc < 1:2111334/69‑1 (MQ=255) gAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATAATCCACGCCCAGAccgccgcc < 1:3354413/69‑1 (MQ=255) gAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATAATCCACGCCCAGAccgccgcc < 1:4162/69‑1 (MQ=255) gAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATAATCCACGCCCAGAccgccgcc < 1:1514907/69‑1 (MQ=255) gAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATAATCCACGCCCAGAccgccgcc < 1:121414/69‑1 (MQ=255) gAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATAATCCACGCCCAGAccgccgcc < 1:2430607/69‑1 (MQ=255) gTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGa > 1:1087814/1‑68 (MQ=255) gTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGa > 1:1183401/1‑68 (MQ=255) gTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGa > 1:927662/1‑68 (MQ=255) gTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGa > 1:2475707/1‑68 (MQ=255) gTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGa > 1:2278498/1‑68 (MQ=255) gTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATAATCCACGCCCAGACAGCCGCCGACGTCGa > 1:1796948/1‑68 (MQ=255) cACCGAACAGTCGGACTGCGAACGAGTAGCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCa > 1:1649022/1‑69 (MQ=255) cACCGAACAGTCGGACTGCGAACGAGTACCTTCATAATCCACGCCCAGAccgccgcc > 1:2519722/1‑57 (MQ=255) cACCGAACAGTCGGACTGCGAACGAGTACCTTCATAATCCACGCCCAGAccgccgcc > 1:1460357/1‑57 (MQ=255) cACCGAACAGTCGGACTGCGAACGAGTACCTTCATAATCCACGCCCAGAccgccgcc > 1:515480/1‑57 (MQ=255) cACCGAACAGTCGGACTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCa > 1:2626575/1‑69 (MQ=255) cACCGAACAGGCGGACTGCGAACGAGTACCTTCATAATCCACGCCCAGAccgccgcc < 1:1597310/57‑1 (MQ=255) aCCGAACAGTCTGACTGCGAACGAGTACCTTCATa < 1:370074/35‑1 (MQ=255) gagcAGTCAGACTGAGAACGGGTGCCTTCATAGTCCACGCCCAGACCGCCGCCGACGTCGAAGCACTg < 1:1375786/65‑1 (MQ=255) gagcAGTCAGACTGAGAACGGGTGCCTTCATAGTCCACGCCCAGACCGCCGCCGACGTCGAAGCACTg < 1:531889/65‑1 (MQ=255) gagcAGTCAGACTGAGAACGGGTGCCTTCATAGTCCACGCCCAGACCGCCGCCGACGTCGAAGCACTg < 1:1458448/65‑1 (MQ=255) gagcAGTCAGACTGAGAACGGGTGCCTTCATAGTCCACGCCCAGACCGCCGCCGACGTCGAAGCACTg < 1:2618990/65‑1 (MQ=255) gagcAGTCAGACTGAGAACGGGTGCCTTCATAGTCCACGCCCAGACCGCCGCCGACGTCGAAGCACTg < 1:854288/65‑1 (MQ=255) gagcAGTCAGACTGAGAACGGGTGCCTTCATAGTCCACGCCCAGACCGCCGCCGACGTCGAAGCACTg < 1:856614/65‑1 (MQ=255) gagcAGTCAGACTGAGAACGGGTGCCTTCATAGTCCACGCCCAGACCGCCGCCGACGTCGAAGCACTg < 1:2578519/65‑1 (MQ=255) gagcAGTCAGACTGAGAACGGGTGCCTTCATAGTCCACGCCCAGACCGCCGCCGACGTCGAAGCACTg < 1:1527328/65‑1 (MQ=255) gagcAGTCAGACTGAGAACGGGTGCCTTCATAGTCCACGCCCAGACCGCCGCCGACGTCGAAGCACTg < 1:2637931/65‑1 (MQ=255) gagcAGTCAGACTGAGAACGGGTGCCTTCATAGTCCACGCCCAGACCGCCGCCGACGTCGAAGCACTg < 1:3027568/65‑1 (MQ=255) gagcAGTCAGACTGAGAACGGGTGCCTTCATAGTCCACGCCCAGACCGCCGCCGACGTCGAAGCACTg < 1:1830043/65‑1 (MQ=255) gagcAGTCAGACTGAGAACGGGTGCCTTCATAGTCCACGCCCAGACCGCCGCCGACGTCGAAGCACTg < 1:3002854/65‑1 (MQ=255) gagcAGTCAGACTGAGAACGGGTGCCTTCATAGTCCACGCCCAGACCGCCGCCGACGTCGAAGCACTg < 1:3262574/65‑1 (MQ=255) gagcAGTCAGACTGAGAACGGGTGCCTTCATAGTCCACGCCCAGACCGCCGCCGACGTCGAAGCACTg < 1:335122/65‑1 (MQ=255) gagcAGTCAGACTGAGAACGGGTGCCTTCATAGTCCACGCCCAGACCGCCGCCGACGTCGAAGCACTg < 1:2245832/65‑1 (MQ=255) gagcAGTCAGACTGAGAACGGGTGCCTTCATAGTCCACGCCCAGACCGCCGCCGACGTCGAAGCACTg < 1:1653784/65‑1 (MQ=255) gagcAGTCAGACTGAGAACGGGTGCCTTCATAGTCCACGCCCAGACCGCCGCCGACGTCGAAGCACTg < 1:2406956/65‑1 (MQ=255) aaCAGTCGGACTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGaa < 1:1393149/69‑1 (MQ=255) aaCAGTCGGACTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGaa < 1:688541/69‑1 (MQ=255) aaCAGTCGGACTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGaa < 1:836408/69‑1 (MQ=255) aaCAGTCGGACTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGaa < 1:1760149/69‑1 (MQ=255) aaCAGTCGGACTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGaa < 1:2539935/69‑1 (MQ=255) gTCGGACTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATAtt < 1:1428845/69‑1 (MQ=255) gTCGGACTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATAtt < 1:62339/69‑1 (MQ=255) gACTGCGAACGAGTACCTTCATAATCCACGCCCAGAccgccgcc < 1:3086174/44‑1 (MQ=255) cTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGcc < 1:2837928/69‑1 (MQ=255) | CCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCC > minE/1971875‑1972005 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |