| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,973,145 | A→G | 26.4% | intergenic (‑85/‑36) | yqgB ← / → yqgC | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,973,145 | 0 | A | G | 26.4% | 51.6 / 16.6 | 49 | intergenic (‑85/‑36) | yqgB/yqgC | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (17/19); new base G (7/6); total (24/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.54e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.76e-01 | |||||||||||
AAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATAACATCGGCAGGTATGCAAAGCAGA > minE/1973080‑1973213 | aaaGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTT‑TTCAGTCCTCAGTAACTGt < 1:2601420/69‑1 (MQ=255) aGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAACTGt < 1:3027411/68‑1 (MQ=255) aGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAACTGt < 1:2278031/68‑1 (MQ=255) aaCCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAGCTGTAACCAGCTCtt < 1:1016008/69‑1 (MQ=255) aaCCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAGCTGTAACCAGCTCtt < 1:653865/69‑1 (MQ=255) aaCCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAGCTGTAACCAGCTCtt < 1:643246/69‑1 (MQ=255) aaCCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAGCTGTAACCAGCTCtt < 1:1365559/69‑1 (MQ=255) aaCCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAGCTGTAACCAGCTCtt < 1:33375/69‑1 (MQ=255) aaCCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAGCTGTAACCAGCTCtt < 1:3016992/69‑1 (MQ=255) aCGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAACTGTAACCAGCTCTTGAAt > 1:2160945/1‑69 (MQ=255) aaCTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAACTGTAACCAGCTCTTGAATCCTgaga > 1:9954/1‑69 (MQ=255) aaCTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAACTGTAACCAGCTCTTGAATCCTgaga > 1:1003265/1‑69 (MQ=255) aaCTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAACTGTAACCAGCTCTTGAATCCTgaga > 1:2458987/1‑69 (MQ=255) aaCTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAACTGTAACCAGCTCTTGAATCCTgaga > 1:2251520/1‑69 (MQ=255) aaCTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAACTGTAACCAGCTCTTGAATCCTgaga > 1:1230735/1‑69 (MQ=255) aaCTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAACTGTAACCAGCTCTTGAATCCTga > 1:2117648/1‑67 (MQ=255) tGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAGCTGTAACCAGctc > 1:1028839/1‑50 (MQ=255) tGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAGCTGTAACCAGctc > 1:2895327/1‑50 (MQ=255) tGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAGCTGTAACCAGctc > 1:2659621/1‑50 (MQ=255) tGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAGCTGTAACCAGctc > 1:577575/1‑50 (MQ=255) tGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAGCTGTAACCAGctc > 1:2302569/1‑50 (MQ=255) tGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAGCTGTAACCAGctc > 1:3307262/1‑50 (MQ=255) tGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAGCTGTAAACAGctc > 1:1877095/1‑50 (MQ=255) ttttACAAGAGCGCCCTTGTTCAGTCCTCAGTAACTGTAACCAGCTCTTGTATCCTGAGAAGCGCCga < 1:1717033/68‑1 (MQ=255) ttttACAAGAGCGCCCTTGTTCAGTCCTCAGTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCga < 1:3215638/68‑1 (MQ=255) ttttACAAGAGCGCCCTTGTTCAGTCCTCAGTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCga < 1:1751044/68‑1 (MQ=255) ttttACAAGAGCGCCCTTGTTCAGTCCTCAGTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCga < 1:297806/68‑1 (MQ=255) ttttACAAGAGCGCCCTTGTTCAGTCCTCAGTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCga < 1:2996414/68‑1 (MQ=255) tttACAAGAGCGCCCTTGTTCAGTCCTCAGTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCgag < 1:3340665/68‑1 (MQ=255) tttACAAGAGCGCCCTTGTTCAGTCCTCAGTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCgag < 1:70219/68‑1 (MQ=255) cccTTGTTCAGTCCTCAGTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATAACAt > 1:569165/1‑69 (MQ=255) cccTTGTTCAGTCCTCAGTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATAACAt > 1:1435594/1‑69 (MQ=255) cccTTGTTCAGTCCTCAGTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATAACAt > 1:2513345/1‑69 (MQ=255) cccTTGTTCAGTCCTCAGTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATAACAt > 1:1577393/1‑69 (MQ=255) cccTTGTTCAGTCCTCAGTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGAATAACAt > 1:2334602/1‑69 (MQ=255) tGTTCAGTCCTCAGTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCgag < 1:453610/52‑1 (MQ=255) tGTTCAGTCCTCAGTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCga < 1:411861/51‑1 (MQ=255) tGTTCAGTCCTCAGTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATAACATCGGc < 1:306500/69‑1 (MQ=255) tGTTCAGTCCTCAGTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATAACATCGGc < 1:991873/69‑1 (MQ=255) tGTTCAGTCCTCAGTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATAACATCGGc < 1:2088834/69‑1 (MQ=255) tGTTCAGTCCTCAGTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATAACATCGGc < 1:1403689/69‑1 (MQ=255) cAGTCCTCAGTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATAACATCGGCAgg < 1:2500186/68‑1 (MQ=255) cAGTCCTCAGTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATAACATCGGCAGGt < 1:524339/69‑1 (MQ=255) cTCAGTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATAACATCGGCAGGTATGCa < 1:3223724/69‑1 (MQ=255) cAGTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATAACATCGGCAGGTATGCaa > 1:1748060/1‑68 (MQ=255) cAGTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATAACATCGGCAGGTATGCaa > 1:1253657/1‑68 (MQ=255) cAGTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATAACATCGGCAGGTATGCaa > 1:1809365/1‑68 (MQ=255) cAGTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATAACATCGGCAGGTATGCaa > 1:833655/1‑68 (MQ=255) aGTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATAACATCGGCAGATATGCaa > 1:1974982/1‑67 (MQ=255) aCTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATAACATCGGCAGGTATGCAAAGCagt > 1:1615917/1‑68 (MQ=255) | AAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAACTGTAACCAGCTCTTGAATCCTGAGAAGCGCCGAGATGGGTATAACATCGGCAGGTATGCAAAGCAGA > minE/1973080‑1973213 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |