| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,973,768 | T→C | 28.6% | I23I (ATT→ATC) | metK → | methionine adenosyltransferase 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,973,768 | 0 | T | C | 28.6% | 146.6 / 65.5 | 143 | I23I (ATT→ATC) | metK | methionine adenosyltransferase 1 |
| Reads supporting (aligned to +/- strand): ref base T (55/47); new base C (18/23); total (73/70) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.55e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
Only 100 of 150 total aligned reads displayed.
ATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTACG > minE/1973700‑1973835 | atGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAAtt > 1:588236/1‑69 (MQ=255) gCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCt < 1:699678/69‑1 (MQ=255) gCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCt < 1:3204500/69‑1 (MQ=255) ccTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGt > 1:2355623/1‑69 (MQ=255) catttttACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGt > 1:70289/3‑69 (MQ=255) tttttACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGt > 1:2266304/1‑67 (MQ=255) tttttACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGt > 1:1815616/1‑67 (MQ=255) tttttACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGt > 1:3327634/1‑67 (MQ=255) tttttACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGt > 1:2510381/1‑67 (MQ=255) tttttACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGt > 1:642115/1‑67 (MQ=255) tttttACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGt > 1:2893627/1‑67 (MQ=255) tttttACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGt > 1:1223672/1‑67 (MQ=255) tACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTa < 1:1844241/67‑1 (MQ=255) tACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTa < 1:2281351/67‑1 (MQ=255) tACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTa < 1:229833/67‑1 (MQ=255) aCGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTa < 1:925903/66‑1 (MQ=255) ccGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGa < 1:2284340/69‑1 (MQ=255) ccGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGa < 1:2442029/69‑1 (MQ=255) tCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAAtt > 1:1824732/1‑42 (MQ=255) tCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCt > 1:1916566/1‑68 (MQ=255) cGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCg > 1:95750/1‑68 (MQ=255) cGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCg > 1:2930873/1‑68 (MQ=255) cGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCg > 1:1095228/1‑68 (MQ=255) cGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCg > 1:876137/1‑68 (MQ=255) cGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCg > 1:1987504/1‑68 (MQ=255) cGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCg > 1:2074468/1‑68 (MQ=255) cGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCg > 1:278101/1‑68 (MQ=255) cGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCg > 1:3148810/1‑68 (MQ=255) ctctGAAGGGCATCCTGACAAAATTGCTGACCAAATCTCTGATGCCGTTTTAGACGCGATCCTCGAAc > 1:3225088/1‑68 (MQ=255) ctctGAAGGGCATCCTGACAAAATTGCTGACCAAATCTCTGATGCCGTTTTAGACGCGATCCTCGAAc > 1:3361734/1‑68 (MQ=255) ctctGAAGGGCATCCTGACAAAATTGCTGACCAAATCTCTGATGCCGTTTTAGACGCGATCCTCGAAc > 1:1024408/1‑68 (MQ=255) ctctGAAGGGCATCCTGACAAAATTGCTGACCAAATCTCTGATGCCGTTTTAGACGCGATCCTCGAAc > 1:2686667/1‑68 (MQ=255) ctctGAAGGGCATCCTGACAAAATTGCTGACCAAATCTCTGATGCCGTTTTAGACGCGATCCTCGAAc > 1:2823263/1‑68 (MQ=255) ctctGAAGGGCATCCTGACAAAATTGCTGACCAAATCTCTGATGCCGTTTTAGACGCGATCCTCGAAc > 1:3000177/1‑68 (MQ=255) ctctGAAGGGCATCCTGACAAAATTGCTGACCAAATCTCTGATGCCGTTTTAGACGCGATCCTCGAAc > 1:38807/1‑68 (MQ=255) ctctGAAGGGCATCCTGACAAAATTGCTGACCAAATCTCTGATGCCGTTTTAGACGCGATCCTCGAAc > 1:1747159/1‑68 (MQ=255) ctctGAAGGGCATCCTGACAAAATTGCTGACCAAATCTCTGATGCCGTTTTAGACGCGATCCTCGAAc > 1:1362765/1‑68 (MQ=255) ctctGAAGGGCATCCTGACAAAATTGCTGACCAAATCTCTGATGCCGTTTTAGACGCGATCCTCGAAc > 1:2212255/1‑68 (MQ=255) ctctGAAGGGCATCCTGACAAAATTGCTGACCAAATCTCTGATGCCGTTTTAGACGCGATCCTCGAAc > 1:470250/1‑68 (MQ=255) ctctGAAGGGCATCCTGACAAAATTGCTGACCAAATCTCTGATGCCGTTTTAGACGCGATCCTCGAAc > 1:1618708/1‑68 (MQ=255) ctctGAAGCGCATCCTGACAAAATTGCTGACCAAATCTCTGATGCCGTTTTAGACGCGATCCTCGAAc > 1:2867260/1‑68 (MQ=255) gggCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATcc > 1:2627890/1‑68 (MQ=255) gggCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATcc > 1:3005971/1‑68 (MQ=255) gggCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATcc > 1:2849832/1‑68 (MQ=255) gggCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATcc > 1:1454779/1‑68 (MQ=255) cATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGaa > 1:1403816/1‑68 (MQ=255) cATCCTGACAAAATT‑CTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAg < 1:3062740/69‑1 (MQ=255) aTCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAg < 1:1483942/69‑1 (MQ=255) aTCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAg < 1:2813108/69‑1 (MQ=255) ccTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCa < 1:2421599/69‑1 (MQ=255) ccTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCa < 1:2522463/69‑1 (MQ=255) ccTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCa < 1:3220731/69‑1 (MQ=255) ccTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCa < 1:1622244/69‑1 (MQ=255) ccTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCa < 1:1227847/69‑1 (MQ=255) cTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCa < 1:2931699/68‑1 (MQ=255) cTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCAc > 1:2134227/1‑69 (MQ=255) cTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCAc > 1:303619/1‑69 (MQ=255) cTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCAc > 1:29371/1‑69 (MQ=255) cTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCAc > 1:980592/1‑69 (MQ=255) cTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCAc > 1:1736184/1‑69 (MQ=255) cTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCAc > 1:2300289/1‑69 (MQ=255) cTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCAc > 1:908518/1‑69 (MQ=255) cTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCAc > 1:1310350/1‑69 (MQ=255) cTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCAc > 1:436680/1‑69 (MQ=255) cTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCAc > 1:2722737/1‑69 (MQ=255) cTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCAc > 1:3185096/1‑69 (MQ=255) cTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCAc > 1:137876/1‑69 (MQ=255) cTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCAc > 1:2358381/1‑69 (MQ=255) cTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCAc > 1:259157/1‑69 (MQ=255) gACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGaa < 1:1039355/69‑1 (MQ=255) gACCAAATCTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGaa < 1:2884534/69‑1 (MQ=255) gACCAAATCTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGaa < 1:1050898/69‑1 (MQ=255) gACCAAATCTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGaa < 1:1198649/69‑1 (MQ=255) gACCAAATCTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGaa < 1:1228223/69‑1 (MQ=255) gACCAAATCTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGaa < 1:923532/69‑1 (MQ=255) gACCAAATCTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGaa < 1:125719/69‑1 (MQ=255) gACCAAATCTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGaa < 1:129852/69‑1 (MQ=255) gACCAAATCTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGaa < 1:14410/69‑1 (MQ=255) gACCAAATCTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGaa < 1:1511076/69‑1 (MQ=255) gACCAAATCTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGaa < 1:164042/69‑1 (MQ=255) gACCAAATCTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGaa < 1:2095563/69‑1 (MQ=255) gACCAAATCTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGaa < 1:3429920/69‑1 (MQ=255) gACCAAATCTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGaa < 1:2422131/69‑1 (MQ=255) gACCAAATCTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGaa < 1:2472261/69‑1 (MQ=255) aCCAAATCTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGaa < 1:901139/68‑1 (MQ=255) aCCAAATCTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGaa < 1:1419366/68‑1 (MQ=255) aCCAAATCTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGaa < 1:3286913/68‑1 (MQ=255) cAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAAcc < 1:2938150/69‑1 (MQ=255) cAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAAc < 1:638202/68‑1 (MQ=255) cAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAAc < 1:1536613/68‑1 (MQ=255) cAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAAc < 1:251356/68‑1 (MQ=255) cAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAAc < 1:2526594/68‑1 (MQ=255) cAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAAc < 1:467010/68‑1 (MQ=255) cAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAAc < 1:2710996/68‑1 (MQ=255) cAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAAc < 1:2789506/68‑1 (MQ=255) cAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAAc < 1:3384725/68‑1 (MQ=255) aaTTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTa < 1:839291/69‑1 (MQ=255) aaTTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTa < 1:2583916/69‑1 (MQ=255) ttCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTACg > 1:616074/1‑68 (MQ=255) ttCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTACg > 1:125457/1‑68 (MQ=255) | ATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTACG > minE/1973700‑1973835 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |