| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,973,851 | T→C | 44.8% | V51A (GTT→GCT) | metK → | methionine adenosyltransferase 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,973,851 | 0 | T | C | 44.8% | 0.2 / 20.2 | 29 | V51A (GTT→GCT) | metK | methionine adenosyltransferase 1 |
| Reads supporting (aligned to +/- strand): ref base T (8/8); new base C (4/9); total (12/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.51e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.04e-01 | |||||||||||
GCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTACGTAAAAACCGGCATGGTTTTAGTTGGCGGCGAAATCACCACCAGCGCCTGGGTAGACATCGAAGAGATCACCCGTAACACCGTT > minE/1973787‑1973918 | gcgATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTAAGTAAAAACCGGCATGGTTTTa > 1:2430401/1‑69 (MQ=255)gcgATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGAGAAACCTACGTAAAAACCGGCATGGCTTTa > 1:1056150/1‑69 (MQ=255) tCCGAAAGCACGCGTTGCTTGCGAAACCTACGTAAAAACCGGCATGGTTTTAGTTGGCGGCGAAATcac < 1:664756/69‑1 (MQ=255) tCCGAAAGCACGCGTTGCTTGCGAAACCTACGTAAAAACCGGCATGGTTTTAGTTGGCGGCGAAATcac < 1:1931702/69‑1 (MQ=255) ccGAAAGCACGCGTTGCTTGCGAAACCTACGTAAAAACCGGCATGGCTTTAGTTGGCGGCGAAATcacc > 1:1025985/1‑69 (MQ=255) ccGAAAGCACGCGTTGCTTGCGAAACCTACGTAAAAACCGGCATGGCTTTAGTTGGCGGCGAAATcacc > 1:1011749/1‑69 (MQ=255) ccGAAAGCACGCGTTGCTTGCGAAACCTACGTAAAAACCGGCATGGCTTTAGTTGGCGGCGAAATcacc > 1:465170/1‑69 (MQ=255) aaaGCACGCGTTGCTTGCGAAACCTACGTAAAAACTGGCATGGTTTTAGTTGGCGGCGAAATCaccacc > 1:2853604/1‑69 (MQ=255) aaaGCACGCGTTGCTTGCGAAACCTACGTAAAAACCGGCATGGTTTTAGTTGGCGGCGAAATCaccacc > 1:55907/1‑69 (MQ=255) aaaGCACGCGTTGCTTGCGAAACCTACGTAAAAACCGGCATGGTTTTAGTTGGCGGCGAAATCaccacc > 1:3277008/1‑69 (MQ=255) aaaGCACGCGTTGCTTGCGAAACCTACGTAAAAACCGGCATGGTTTTAGTTGGCGGCGAAATCaccacc > 1:2455565/1‑69 (MQ=255) cgTTGCTTGCGAAACCTACGTAAAAACCGGCATGGTTTTAGTTGGCGGCGAAATCACCACCAGCGCCTg < 1:3259929/69‑1 (MQ=255) gcttgcGAAACCTACGTAAAAACCGGCATGGTTTTAGTTGGCGGCGAAATCACCACCAGCGCCTGGGTa < 1:3072358/69‑1 (MQ=255) gcttgcGAAACCTACGTAAAAACCGGCATGGTTTTAGTTGGCGGCGAAATCACCACCAGCGCCTGGGTa < 1:2777578/69‑1 (MQ=255) gcttgcGAAACCTACGTAAAAACCGGCATGGTTTTAGTTGGCGGCGAAATCACCACCAGCGCCTGGGTa > 1:3243125/1‑69 (MQ=255) gcttgcGAAACCTACGTAAAAACCGGCATGGTTTTAGTTGGCGGCGAAATCACCACCAGCGCCTGGGTa < 1:1968709/69‑1 (MQ=255) gcttgcGAAACCTACGTAAAAACCGGCATGGTTTTAGTTGGCGGCGAAATCACCACCAGCGCCTGGGTa > 1:455645/1‑69 (MQ=255) gcttgcGAAACCTACGTAAAAACCGGCATGGTTTTAGTTGGCGGCGAAATCACCACCAGCGCCTGGGTa < 1:516722/69‑1 (MQ=255) aCCGGCATGGTTTTAGTTGGCGGCGAAATCACCACCAGCGCCTGGGTAGACATCGAAGAGATCACCCGt < 1:812531/69‑1 (MQ=255) ggCATGGCTTTAGTTGGCGGCGAAATCACCACCAGCGCCTGGGTAGACATCGAAGAGATCACCCGTAac < 1:2541634/69‑1 (MQ=255) ggCATGGCTTTAGTTGGCGGCGAAATCACCACCAGCGCCTGGGTAGACATCGAAGAGATCACCCGTAac < 1:2488214/69‑1 (MQ=255) ggCATGGCTTTAGTTGGCGGCGAAATCACCACCAGCGCCTGGGTAGACATCGAAGAGATCACCCGTAac < 1:244055/69‑1 (MQ=255) ggCATGGCTTTAGTTGGCGGCGAAATCACCACCAGCGCCTGGGTAGACATCGAAGAGATCACCCGTAac < 1:1961027/69‑1 (MQ=255) ggCATGGCTTTAGTTGGCGGCGAAATCACCACCAGCGCCTGGGTAGACATCGAAGAGATCACCCGTAac < 1:1812028/69‑1 (MQ=255) ggCATGGCTTTAGTTGGCGGCGAAATCACCACCAGCGCCTGGGTAGACATCGAAGAGATCACCCGTAac < 1:1695379/69‑1 (MQ=255) ggCATGGCTTTAGTTGGCGGCGAAATCACCACCAGCGCCTGGGTAGACATCGAAGAGATCACCCGTAac < 1:1185533/69‑1 (MQ=255) ggCATGGCTTTAGTTGGCGGCGAAATCACCACCAGCGCCTGGGTAGACATCGAAGAGATCACCCGTAac < 1:743670/69‑1 (MQ=255) ggCATGGCTTTAGTTGGCGGCGAAATCACCACCAGCGCCTGGGTAGACATCGAAGAGATCACCCGTAac < 1:1130292/69‑1 (MQ=255) gTTTTAGTTGGCGGCGAAATCACCACCAGCGCCTGGGTAGACATCGAAGAGATCACCCGTAACACCGtt > 1:174399/1‑69 (MQ=255) | GCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTACGTAAAAACCGGCATGGTTTTAGTTGGCGGCGAAATCACCACCAGCGCCTGGGTAGACATCGAAGAGATCACCCGTAACACCGTT > minE/1973787‑1973918 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |