| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,974,996 | A→T | 47.3% | intergenic (+142/‑282) | metK → / → galP | methionine adenosyltransferase 1/D‑galactose transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,974,996 | 0 | A | T | 47.3% | 2.3 / 63.0 | 55 | intergenic (+142/‑282) | metK/galP | methionine adenosyltransferase 1/D‑galactose transporter |
| Reads supporting (aligned to +/- strand): ref base A (24/5); new base T (13/13); total (37/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.00e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01 | |||||||||||
AAAACCGCCCGCACAATAACATCATTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAACCGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGTT > minE/1974935‑1975062 | aaaaCCGCCCGCACAATAACATCATTCTTCCTCAACACGTTTTACCGCAAATTATCATCACTACTGaaa > 1:2393530/1‑69 (MQ=255)aaaaCCGCCCGCACAATAACATCATTCTTCCTCAACACGTTTTACCGCAAATTATCATCACTACTGaaa > 1:1061845/1‑69 (MQ=255)aaaaCCGCCCGCACAATAACATCATTCTTCCTCAACACGTTTTACCGCAAATTATCATCACTACTGaaa > 1:938324/1‑69 (MQ=255)aaaaCCGCCCGCACAATAACATCATTCTTCCTCAACACGTTTTACCGCAAATTATCATCACTACTGaaa > 1:2544919/1‑69 (MQ=255)aaaaCCGCCCGCACAATAACATCATTCTTCCTCAACACGTTTTACCGCAAATTATCATCACTACTGaaa > 1:1983160/1‑69 (MQ=255)aaaaCCGCCCGCACAATAACATCATTCTTCCTCAACACGTTTTACCGCAAATTATCATCACTACTGaaa > 1:1664505/1‑69 (MQ=255)aaaaCCGCCCGCACAATAACATCATTCTTCCTCAACACGTTTTACCGCAAATTATCATCACTACTGaaa > 1:1903750/1‑69 (MQ=255)aaaaCCGCCCGCACAATAACATCATTCTTCCTCAACACGTTTTACCGCAAATTATCATCACTACTGaaa > 1:2769901/1‑69 (MQ=255)aaaaCCGCCCGCACAATAACATCATTCTTCCTCAACACGTTTTACCGCAAATTATCATCACTACTGaaa > 1:3112938/1‑69 (MQ=255)aaaaCCGCCCGCACAATAACATCATTCTTCCTCAACACGTTTTACCGCAAATTATCATCACTACTGaa > 1:101047/1‑68 (MQ=255)aaaaCCGCCCGCACAATAACATCATTCTTCCTCAACACGTTTTACCGCAAATTATCATCACTACTGaa > 1:2626457/1‑68 (MQ=255)aaaaCCGCCCGCACAATAACATCATTCTTCCTCAACACGTTTTACCGCAAATTATCATCACTACTGaa > 1:2895079/1‑68 (MQ=255)aaaaCCGCCCGCACAATAACATCATTCTTCCTAAACACGTTTTACCGCAAATTATCATCACTACTGaaa > 1:1789957/1‑69 (MQ=255) aTAACATCATTCTTCCTCAACACGTTTTACCGCAAATTATCATCACTACTGAAATCGATTACaccaacc < 1:3222793/69‑1 (MQ=255) aTAACATCATTCTTCCTCAACACGTTTTACCGCAAATTATCATCACTACTGAAATCGATTACaccaacc < 1:470576/69‑1 (MQ=255) aTAACATCATTCTTCCTCAACACGTTTTACCGCAAATTATCATCACTACTGAAATCGATTACaccaacc < 1:678467/69‑1 (MQ=255) aTAACATCATTCTTCCTCAACACGTTTTACCGCAAATTATCATCACTACTGAAATCGATTACaccaacc < 1:2614908/69‑1 (MQ=255) aTAACATCATTCTTCCTCAACACGTTTTACCGCAAATTATCATCACTACTGAAATCGATTACaccaacc < 1:893935/69‑1 (MQ=255) aTAACATCATTCTTCCTCAACACGTTTTACCGCAAATTATCATCACTACTGAAATCGATTACaccaacc < 1:24527/69‑1 (MQ=255) aTAACATCATTCTTCCTCAACACGTTTTACCGCAAATTATCATCACTACTGAAATCGATTACaccaacc < 1:1060156/69‑1 (MQ=255) aTAACATCATTCTTCCTCAACACGTTTTACCGCAAATTATCATCACTACTGAAATCGATTACaccaacc < 1:2239265/69‑1 (MQ=255) aTAACATCATTCTTCCTCAACACGTTTTACCGCAAATTATCATCACTACTGAAATCGATTACaccaacc < 1:1472786/69‑1 (MQ=255) aTAACATCATTCTTCCTCAACACGTTTTACCGCAAATTATCATCACTACTGAAATCGATTACaccaacc < 1:2159775/69‑1 (MQ=255) aTAACATCATTCTTCCTCAACACGTTTTACCGCAAATTATCATCACTACTGAAATCGATTACaccaacc < 1:1607541/69‑1 (MQ=255) aTAACATCATTCTTCCTCAACACGTTTTACCGCAAATTATCATCACTACTGAAATCGATTACaccaacc < 1:1810658/69‑1 (MQ=255) catTCTTCCTCAACACGTTTTACCGCAAATTATCATCACTACTGAAATCGATTACaccaacc < 1:2110184/62‑1 (MQ=255) atTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAACCGAt > 1:3281987/1‑51 (MQ=255) atTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAACCGATTACACCAACCACAACAg > 1:2188825/1‑68 (MQ=255) atTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAACCGATTACACCAACCACAACAg > 1:856574/1‑68 (MQ=255) atTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAACCGATTACACCAACCACAACAg > 1:605419/1‑68 (MQ=255) atTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAACCGATTACACCAACCACAACAg > 1:460813/1‑68 (MQ=255) atTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAACCGATTACACCAACCACAACAg > 1:3432145/1‑68 (MQ=255) atTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAACCGATTACACCAACCACAACAg > 1:3330079/1‑68 (MQ=255) atTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAACCGATTACACCAACCACAACAg > 1:3291119/1‑68 (MQ=255) atTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAACCGATTACACCAACCACAACAg > 1:1843897/1‑68 (MQ=255) atTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAACCGATTACACCAACCACAACAg > 1:2965475/1‑68 (MQ=255) atTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAACCGATTACACCAACCACAACAg > 1:2000441/1‑68 (MQ=255) atTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAACCGATTACACCAACCACAACAg > 1:2204499/1‑68 (MQ=255) atTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAACCGATTACACCAACCACAACAg > 1:2270154/1‑68 (MQ=255) cACGTTTCACCGCAGATTATCATCACAACTGAAACCGATTACACCCACCACAACAGACAAAGATTTGt > 1:1437719/1‑68 (MQ=255) cACGTTTCACCGCAGATTATCATCACAACTGAAACCGATTACACCAACCACAACAGACAAAGATTTGt > 1:3025029/1‑68 (MQ=255) cACGTTTCACCGCAGATTATCATCACAACTGAAACCGATTACACCAACCACAACAGACAAAGATTTGt > 1:3173152/1‑68 (MQ=255) cACGTTTCACCGCAGATTATCATCACAACTGAAACCGATTACACCAACCACAACAGACAAAGATTTGt > 1:96273/1‑68 (MQ=255) cACGTTTCACCGCAGATTATCATCACAACTGAAACCGATTACACCAACCACAACAGACAAAGATTTGt > 1:951399/1‑68 (MQ=255) cACGTTTCACCGCAGATTATCATCACAACTGAAACCGATTACACCAACCACAACAGACAAAGATTTGt > 1:445384/1‑68 (MQ=255) cACGTTTCACCGCAGATTATCATCACAACTGAAACCGATTACACCAACCACAACAGACAAAGATTTGt > 1:484129/1‑68 (MQ=255) tCACCGCAGATTATCATCACAACTGAAACCGATTACACCAACCACAACAGACAAAGATTTGTAATAtt > 1:748417/1‑68 (MQ=255) aCCGCAGATTATCATCACAACTGAAACCGATTACACCAACCACAACAGACAAAGATTTGTAATAtttt < 1:97645/68‑1 (MQ=255) cGCAGATTATCATCACAACTGAAACCGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCa < 1:2261932/68‑1 (MQ=255) cAGATTATCATCACAACTGAAACCGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCatat < 1:3382747/69‑1 (MQ=255) aTTATCATCACAACTGAAACCGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCAtattat < 1:2479531/69‑1 (MQ=255) aTTATCATCACAACTGAAACCGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCAtattat < 1:1746101/69‑1 (MQ=255) ttATCATCACAACTGAAACCGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCAtattatt > 1:1857146/1‑69 (MQ=255) ttATCATCACAACTGAAAACGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCAtattatt > 1:2498327/1‑69 (MQ=255) tATCATCACAACTGAAACCGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCAtattatta > 1:479132/1‑69 (MQ=255) acaACTGAAACCGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGtt > 1:404936/1‑69 (MQ=255) acaACTGAAACCGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGtt > 1:384260/1‑69 (MQ=255) acaACTGAAACCGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGtt > 1:1277624/1‑69 (MQ=255) acaACTGAAACCGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGt > 1:204252/1‑68 (MQ=255) acaACTGAAACCGATGACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGtt > 1:1937623/1‑69 (MQ=255) | AAAACCGCCCGCACAATAACATCATTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAACCGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGTT > minE/1974935‑1975062 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |