| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 157,729 | A→G | 34.2% | L84L (TTG→CTG) | pfs ← | 5'‑methylthioadenosine/S‑adenosylhomocysteine nucleosidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 157,729 | 0 | A | G | 34.2% | 50.3 / 40.4 | 73 | L84L (TTG→CTG) | pfs | 5'‑methylthioadenosine/S‑adenosylhomocysteine nucleosidase |
| Reads supporting (aligned to +/- strand): ref base A (22/26); new base G (5/20); total (27/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.12e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.01e-01 | |||||||||||
ACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCACTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAAC > minE/157661‑157793 | aCCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCACTTTCaa < 1:2974186/69‑1 (MQ=255)aCCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCACTTTCaa < 1:20468/69‑1 (MQ=255) aaaTGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCACTTTCAACGt < 1:2863678/69‑1 (MQ=255) aaaTGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCACTTTCAACGt < 1:1195611/69‑1 (MQ=255) aaaTGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCACTTTCAACGt < 1:2707928/69‑1 (MQ=255) aaaTGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCACTTTCAACGt < 1:1958100/69‑1 (MQ=255) aaTGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCACTTTCAACGtt < 1:1390358/69‑1 (MQ=255) cgcgTCGTTATAACGTGCTTCGTCCGAGACAACGATATCGCCCACTTTCAACGt < 1:2079404/54‑1 (MQ=255) cgcgTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCACTTTCAACGt < 1:1001551/54‑1 (MQ=255) cgcgTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCACTTTCAACGt < 1:3235663/54‑1 (MQ=255) cgcgTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCACTTTCAACGt < 1:3112329/54‑1 (MQ=255) cgcgTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCACTTTCAACGt < 1:3319510/54‑1 (MQ=255) cgcgTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCACTTTCAACGt < 1:2196006/54‑1 (MQ=255) cgcgTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCACTTTCAACGt < 1:1820346/54‑1 (MQ=255) cgcgTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCACTTTCAACGt < 1:1679402/54‑1 (MQ=255) cgcgTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCACTTTCAACGt < 1:1612760/54‑1 (MQ=255) cgcgTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCACTTTCAACGt < 1:621037/54‑1 (MQ=255) cgcgTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCACTTTCAACGt < 1:689066/54‑1 (MQ=255) cgtcgtGATAACGTGCTTCGTCCGAGACAACGATATCGCCCACTTTCAACGt < 1:2902999/52‑1 (MQ=255) gtGATAACGTGCTTCGTCCGAGACAACGATATCGCCCACTTTCAACGTTGGTGCCAGGCCACCg > 1:2114857/1‑64 (MQ=255) gtGATAACGTGCTTCGTCCGAGACAACGATATCGCCCACTTTCAACGTTGGTGCCAGGCCACCGGCAGa > 1:1844255/1‑69 (MQ=255) gtGATAACGTGCTTCGTCCGAGACAACGATATCGCCCACTTTCAACGTTGGTGCCAGGCCACCGGCAGa > 1:3087728/1‑69 (MQ=255) gtGATAACGTGCTTCGTCCGAGACAACGATATCGCCCACTTTCAACGTTGGTGCCAGGCCACCGGCAGa > 1:2925442/1‑69 (MQ=255) gtGATAACGTGCTTCGTCCGAGACAACGATATCGCCCACTTTCAACGTTGGTGCCAGGCCACCGGCAGa > 1:734573/1‑69 (MQ=255) aTAACGTGCTTCGTCCGAGACAACGATATCGCCCACTTTCaa < 1:3122804/42‑1 (MQ=255) gcgCTTCGTCCGAGACATCGATATCGCCCACTTTCAGCGTTCGTGCCAGGCCACCGGCAGAACCgg > 1:1260654/3‑66 (MQ=255) gcgCTTCGTCCGAGACAACGATATCGCCCACTTTCAGCGTTGGTGCCAGGCCACCGGCAGAACCGgtg > 1:321194/3‑68 (MQ=255) gcgCTTCGTCCGAGACAACGATATCGCCCACTTTCAGCGTTGGTGCCAGGCCACCGGCAGAACCGgtg > 1:2507543/3‑68 (MQ=255) gcgCTTCGTCCGAGACAACGATATCGCCCACTTTCAGCGTTGGTGCCAGGCCACCGGCAGAACCGgt > 1:1753819/3‑67 (MQ=255) gcgCTTCGTCCGAGACAACGATATCGCCCACTTTCAGCGTTGGTGCCAGGCCACCGGCAGAACCGgt > 1:3001061/3‑67 (MQ=255) tCGTCCGAGACAACGATATCGCCCACTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTtaat < 1:1583557/68‑1 (MQ=255) tCGTCCGAGACAACGATATCGCCCACTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTtaat < 1:317702/68‑1 (MQ=255) tCGTCCGAGACAACGATATCGCCCACTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTtaat < 1:1181939/68‑1 (MQ=255) agaCAACGATATCGCCCACTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATcaca < 1:744838/68‑1 (MQ=255) agaCAACGATATCGCCCACTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATcaca < 1:2147291/68‑1 (MQ=255) agaCAACGATATCGCCCACTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATcaca < 1:2680132/68‑1 (MQ=255) agaCAACGATATCGCCCACTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATcaca < 1:2628875/68‑1 (MQ=255) ctcTTTCAGCGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGtt < 1:322275/66‑1 (MQ=255) aCTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGTCTTGCAGTGTTcc < 1:3316314/69‑1 (MQ=255) aCTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGTCTTGCAGTGTTcc < 1:3312242/69‑1 (MQ=255) ttCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAg > 1:2342714/1‑60 (MQ=255) ttCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAAc > 1:2994537/1‑69 (MQ=255) ttCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAAc > 1:697848/1‑69 (MQ=255) ttCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAAc > 1:2282311/1‑69 (MQ=255) ttCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAAc > 1:1903546/1‑69 (MQ=255) ttCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAAc > 1:1081938/1‑69 (MQ=255) ttCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAAc > 1:1967525/1‑69 (MQ=255) ttCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAAc > 1:3273717/1‑69 (MQ=255) ttCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAAc > 1:2594424/1‑69 (MQ=255) ttCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAAc > 1:456551/1‑69 (MQ=255) ttCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAAc > 1:3290046/1‑69 (MQ=255) ttCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAAc > 1:2911661/1‑69 (MQ=255) ttCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAAc > 1:1412672/1‑69 (MQ=255) ttCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAAc > 1:2558182/1‑69 (MQ=255) ttCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAAc > 1:2833261/1‑69 (MQ=255) ttCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAAc > 1:2573891/1‑69 (MQ=255) ttCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAAAAATCACATCTGGCTTGCAGTGTTCCAAc > 1:3070270/1‑69 (MQ=255) tCAGCGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGtt < 1:597712/63‑1 (MQ=255) tCAGCGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGtt < 1:808327/63‑1 (MQ=255) tCAGCGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGtt < 1:350210/63‑1 (MQ=255) tCAGCGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGtt < 1:3208997/63‑1 (MQ=255) tCAGCGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGtt < 1:3198449/63‑1 (MQ=255) tCAGCGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGtt < 1:2912571/63‑1 (MQ=255) tCAGCGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGtt < 1:225440/63‑1 (MQ=255) tCAGCGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGtt < 1:2240952/63‑1 (MQ=255) tCAGCGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGtt < 1:2239927/63‑1 (MQ=255) tCAGCGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGtt < 1:2085322/63‑1 (MQ=255) tCAGCGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGtt < 1:2026004/63‑1 (MQ=255) tCAGCGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGtt < 1:2023584/63‑1 (MQ=255) tCAGCGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGtt < 1:145933/63‑1 (MQ=255) tCAGCGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGtt < 1:1332970/63‑1 (MQ=255) tCAGCGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGtt < 1:1321194/63‑1 (MQ=255) tCAGCGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGtt < 1:129603/63‑1 (MQ=255) tCAGCGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGtt < 1:1103183/63‑1 (MQ=255) tCAGCGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGtt < 1:1057948/63‑1 (MQ=255) tCAGCGTTGGTGCCAGGCCACCGGCAGAACCGGGGTTAATAATCACATCTGGCTTGCAGTGtt < 1:3075452/63‑1 (MQ=255) | ACCAAATGCCGTGACATCCGCGTCGTGATAACGTGCTTCGTCCGAGACAACGATATCGCCCACTTTCAACGTTGGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAAC > minE/157661‑157793 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |