| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,977,610 | G→A | 53.8% | Q90Q (CAG→CAA) | endA → | DNA‑specific endonuclease I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,977,610 | 0 | G | A | 53.8% | ‑0.3 / 30.5 | 39 | Q90Q (CAG→CAA) | endA | DNA‑specific endonuclease I |
| Reads supporting (aligned to +/- strand): ref base G (6/12); new base A (12/9); total (18/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.01e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATCAGGTGCGCAAAAATGAAAACCGCGCCAGCCGCGTAGAGTGGGAACATGTCGTTCCCGCCTGGCAGTTCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAACTGCGCTAAAGATC > minE/1977543‑1977668 | aTCAGGTGCGCAAAAATGAAAACCGCGCCAGCCGCGTAGAGTGGGAACATGTCGTTCCCGCCTGGCAGt < 1:1912733/69‑1 (MQ=255)aTCAGGTGCGCAAAAATGAAAACCGCGCCAGCCGCGTAGAGTGGGAACATGTCGTTCCCGCCTGGCAGt < 1:3318374/69‑1 (MQ=255) aaTGAAAACCGCGCCAGCCGCGTAGAGTGGGAACATGTCGTTCCCGCCTGGCAGTTCGGTCACCAgcgc < 1:1679913/69‑1 (MQ=255) aaTGAAAACCGCGCCAGCCGCGTAGAGTGGGAACATGTCGTTCCCGCCTGGCAGTTCGGTCACCAgcgc < 1:1932515/69‑1 (MQ=255) aGCCGCGTAGAGTGGGAACATGTCGTTCCCGCCTGGCAGTTCGGTCACCAGCGCCAGTGCTGGCAGGAc < 1:94058/69‑1 (MQ=255) aGCCGCGTAGAGTGGGAACATGTCGTTCCCGCCTGGCAGTTCGGGCACCAGCGCCAGTGCTGGCAGGAc < 1:649076/69‑1 (MQ=255) aGCCGCGTAGAGTGGGAACATGTCGTTCCCGCCTGGCAGTGCGGTCACCAGCGCCAGTGCTGGCAGAAc < 1:1894131/69‑1 (MQ=255) gCCGCGTAGAGTGGGAACATGTCGTTCCCGCCTGGCAGTTCGGTCACCAGCGCCAGTGCTGGCAGGAc < 1:1638988/68‑1 (MQ=255) gCCGCGTAGAGTGGGAACATGTCGTTCCCGCCTGGCAGTTCGGTCACCAGCGCCAGTGCTGGCAGGAc < 1:463941/68‑1 (MQ=255) tCGTTCCCGCCTGGCAGTTCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAACTgcgc > 1:798324/1‑67 (MQ=255) tCCTGCCTGGCAATTCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAATTGCGCCAAAg < 1:1299929/68‑1 (MQ=255) tCCTGCCTGGCAATTCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAATTGCGCCAAAg < 1:336609/68‑1 (MQ=255) tCCTGCCTGGCAATTCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAATTGCGCCAAAg < 1:3052540/68‑1 (MQ=255) tCCTGCCTGGCAATTCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAATTGCGCCAAAg < 1:2981921/68‑1 (MQ=255) tCCTGCCTGGCAATTCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAATTGCGCCAAAg < 1:277629/68‑1 (MQ=255) tCCTGCCTGGCAATTCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAATTGCGCCAAAg < 1:2190982/68‑1 (MQ=255) tCCTGCCTGGCAATTCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAATTGCGCCAAAg < 1:1016460/68‑1 (MQ=255) tCCTGCCTGGCAATTCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAATTGCGCCAAAg < 1:212477/68‑1 (MQ=255) tCCTGCCTGGCAATTCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAATTGCGCCAAAg < 1:2253654/68‑1 (MQ=255) tCCCGCCTGGCAGTTCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAACTGCGCTAAAg < 1:177081/68‑1 (MQ=255) tCCCGCCTGGCAGTTCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAACTGCGCTAAAg < 1:2478304/68‑1 (MQ=255) tCCCGCCTGGCAGTTCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAACTGCGCTAAAg < 1:3360546/68‑1 (MQ=255) cctgCCTGGCAATTCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAATTGCGCCAAAGa > 1:2815313/4‑68 (MQ=255) cctgCCTGGCAATTCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAATTGCGCCAAAGAt > 1:2287174/4‑69 (MQ=255) cctgCCTGGCAATTCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAATTGCGCCAAAGAt > 1:1515058/4‑69 (MQ=255) cctgCCTGGCAATTCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAATTGCGCCAAAGAt > 1:796518/4‑69 (MQ=255) cctgCCTGGCAATTCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAATTGCGCCAAAGAt > 1:393844/4‑69 (MQ=255) cctgCCTGGCAATTCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAATTGCGCCAAAGAt > 1:345329/4‑69 (MQ=255) cctgCCTGGCAATTCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAATTGCGCCAAAGAt > 1:2535460/4‑69 (MQ=255) cctgCCTGGCAATTCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAATTGCGCCAAAGAt > 1:3279709/4‑69 (MQ=255) cctgCCTGGCAATTCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAATTGCGCCAAAGAt > 1:1347218/4‑69 (MQ=255) cctgCCTGGCAATTCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAATTGCGCCAAAGAt > 1:2269356/4‑69 (MQ=255) cctgCCTGGCAATTCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAATTGCGCCAAAGAt > 1:2275601/4‑69 (MQ=255) cctgCCTGGCAATTCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAATTGCGCCAAAGAt > 1:234160/4‑69 (MQ=255) cccGCCTTGCAGTTCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAACTGCGCTAAAGAt > 1:559369/1‑69 (MQ=255) cccGCCTGGCAGTTCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAACTGCGCTAAAGAt > 1:2428011/1‑69 (MQ=255) cccGCCTGGCAGTTCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAACTGCGCTAAAGAt > 1:3075075/1‑69 (MQ=255) cccGCCTGGCAGTTCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAACTGCGCTAAAGAt > 1:2562089/1‑69 (MQ=255) cccGCCTGGCAGTTCGGTCACCAGCGCCAGTGCTGGCAGGA‑GGTGGACGTAAAAACTGCGCTAAAGATc > 1:56857/1‑69 (MQ=255) | ATCAGGTGCGCAAAAATGAAAACCGCGCCAGCCGCGTAGAGTGGGAACATGTCGTTCCCGCCTGGCAGTTCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAACTGCGCTAAAGATC > minE/1977543‑1977668 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |