| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,977,655 | C→T | 41.9% | N105N (AAC→AAT) | endA → | DNA‑specific endonuclease I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,977,655 | 0 | C | T | 41.9% | 63.4 / 114.2 | 155 | N105N (AAC→AAT) | endA | DNA‑specific endonuclease I |
| Reads supporting (aligned to +/- strand): ref base C (39/51); new base T (12/53); total (51/104) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.67e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01 | |||||||||||
Only 100 of 170 total aligned reads displayed.
TCGTTCCCGCCTGGCAGTTCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAACTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGATATGCATAACCTGCAGCCGTCAGTCGGTGA > minE/1977594‑1977723 | tCGTTCCCGCCTGGCAGTTCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAACTgcgc > 1:798324/1‑67 (MQ=255) tCCTGCCTGGCAATTCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAATTGCGCCAAAg < 1:2190982/68‑1 (MQ=255) tCCTGCCTGGCAATTCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAATTGCGCCAAAg < 1:336609/68‑1 (MQ=255) tCCTGCCTGGCAATTCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAATTGCGCCAAAg < 1:2253654/68‑1 (MQ=255) tCCTGCCTGGCAATTCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAATTGCGCCAAAg < 1:3052540/68‑1 (MQ=255) tCCTGCCTGGCAATTCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAATTGCGCCAAAg < 1:2981921/68‑1 (MQ=255) tCCTGCCTGGCAATTCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAATTGCGCCAAAg < 1:1299929/68‑1 (MQ=255) tCCTGCCTGGCAATTCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAATTGCGCCAAAg < 1:1016460/68‑1 (MQ=255) tCCCGCCTGGCAGTTCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAACTGCGCTAAAg < 1:2478304/68‑1 (MQ=255) cctgCCTGGCAATTCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAATTGCGCCAAAGa > 1:2815313/4‑68 (MQ=255) cctgCCTGGCAATTCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAATTGCGCCAAAGAt > 1:345329/4‑69 (MQ=255) cctgCCTGGCAATTCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAATTGCGCCAAAGAt > 1:2275601/4‑69 (MQ=255) cctgCCTGGCAATTCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAATTGCGCCAAAGAt > 1:234160/4‑69 (MQ=255) cctgCCTGGCAATTCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAATTGCGCCAAAGAt > 1:393844/4‑69 (MQ=255) cccGCCTTGCAGTTCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAACTGCGCTAAAGAt > 1:559369/1‑69 (MQ=255) cccGCCTGGCAGTTCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAACTGCGCTAAAGAt > 1:2562089/1‑69 (MQ=255) tCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAATTGCGCCAAAGATc < 1:2502014/57‑1 (MQ=255) tCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAATTGCGCCAAAGATc < 1:2621409/57‑1 (MQ=255) tCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAATTGCGCCAAAGATc < 1:2752223/57‑1 (MQ=255) tCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAATTGCGCCAAAGATc < 1:2283613/57‑1 (MQ=255) tCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAATTGCGCCAAAGATc < 1:369139/57‑1 (MQ=255) tCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAATTGCGCCAAAGATc < 1:3110976/57‑1 (MQ=255) tCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAATTGCGCCAAAGATc < 1:3272718/57‑1 (MQ=255) tCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAATTGCGCCAAAGATc < 1:2243254/57‑1 (MQ=255) tCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAATTGCGCCAAAGATc < 1:170920/57‑1 (MQ=255) tCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAATTGCGCCAAAGATc < 1:1439640/57‑1 (MQ=255) tCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAATTGCGCCAAAGATc < 1:1393544/57‑1 (MQ=255) tCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAATTGCGCCAAAGATc < 1:1385774/57‑1 (MQ=255) tCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAATTGCGCCAAAGATc < 1:734577/57‑1 (MQ=255) tCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAATTGCGCCAAAGATc < 1:777226/57‑1 (MQ=255) cGGTCACCAGCGCGAGTGCTGGCAGGACGGTGGACGTAAAAATTGCGCCAAAGATc < 1:1494632/56‑1 (MQ=255) cGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAACTGCGCTAAAGATCCGGTCTATc > 1:2641147/1‑65 (MQ=255) cGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAACTGCGCTAAAGATCCGGTCTATc > 1:1845442/1‑65 (MQ=255) cGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAACTGCGCTAAAGATCCGGTCTATc > 1:318630/1‑65 (MQ=255) cGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAACTGCGCTAAAGATCCGGTCTATc > 1:1948746/1‑65 (MQ=255) cGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAACTGCGCTAAAGATCCGGTCTATc > 1:2148174/1‑65 (MQ=255) cGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAACTGCGCTAAAGATCCGGTCAATc > 1:2799235/1‑65 (MQ=255) cAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAACTGCGCTAAAGATCCGGTCTATCGCAAGATGGaa > 1:2894710/1‑69 (MQ=255) cAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAACTGCGCTAAAGATCCGGTCTATCGCAAGATGGaa > 1:833108/1‑69 (MQ=255) cAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAACTGCGCTAAAGATCCGGTCTATCGCAAGATGGaa > 1:3382487/1‑69 (MQ=255) cAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAACTGCGCTAAAGATCCGGTCTATCGCAAGATGGaa > 1:576707/1‑69 (MQ=255) cAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAACTGCGCTAAAGATCCGGTCTATCGCAAGATGGa < 1:1767217/68‑1 (MQ=255) ccAGTGCTGGCAGGACGGTGGACGTAAAAACTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGa < 1:3414062/69‑1 (MQ=255) ccAGTGCTGGCAGGACGGTGGACGTAAAAACTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGa < 1:1341472/69‑1 (MQ=255) ccAGTGCTGGCAGGACGGTGGACGTAAAAACTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGa < 1:966673/69‑1 (MQ=255) ccAGTGCTGGCAGGACGGTGGACGTAAAAACTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGa < 1:925781/69‑1 (MQ=255) ccAGTGCTGGCAGGACGGTGGACGTAAAAACTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGa < 1:543303/69‑1 (MQ=255) ccAGTGCTGGCAGGACGGTGGACGTAAAAACTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGa < 1:2504773/69‑1 (MQ=255) ccAGTGCTGGCAGGACGGTGGACGTAAAAACTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGa < 1:478079/69‑1 (MQ=255) ccAGTGCTGGCAGGACGGTGGACGTAAAAACTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGa < 1:1612419/69‑1 (MQ=255) ccAGTGCTGGCAGGACGGTGGACGTAAAAACTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGa < 1:1649485/69‑1 (MQ=255) cAGTGCTGGCAGGACGGTGGACGTAAAAACTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGat < 1:2318534/69‑1 (MQ=255) cAGTGCTGGCAGGACGGTGGACGTAAAAACTGCGATAAAGATCCGGTCTATCGCAAGATGGAAAGCGa < 1:2995638/68‑1 (MQ=255) gTGCTGGCAGGACGGTGGACGTAAAAATTGCGCCAAAGATc < 1:2943430/41‑1 (MQ=38) gCTGGCAGGACGGTGGACGTAAAAACTGCGCTAAAg > 1:1726278/1‑36 (MQ=255) cTGGCAGGACGGTGGACGTAAAAACTGCGCTAAAGATCCGGTCt > 1:1485080/1‑44 (MQ=255) cTGGCAGGACGGTGGACGTAAAAACTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGATATGc > 1:2955833/1‑68 (MQ=255) cTGGCAGGACGGTGGACGTAAAAACTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGATATGc > 1:64577/1‑68 (MQ=255) ggCAGGACGGTGGACGTAAAAACTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGATATGCATa < 1:2212383/69‑1 (MQ=255) ggCAGGACGGTGGACGTAAAAACTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGATATGCATa < 1:151785/69‑1 (MQ=255) ggCAGGACGGTGGACGTAAAAACTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGATATGCATa < 1:3363280/69‑1 (MQ=255) ggCAGGACGGTGGACGTAAAAACTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGATATGCATa < 1:431469/69‑1 (MQ=255) gCAGGACGGTGGACGTAAAAACTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGATATGCATa < 1:2856732/68‑1 (MQ=255) gggtggtCGTAAAATCTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCgt < 1:710054/53‑2 (MQ=255) ggTGGACGTAAAAACTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAATCGa > 1:1451849/1‑53 (MQ=255) ggTGGACGTAAAAACTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGa < 1:690662/53‑1 (MQ=255) ggTGGACGTAAAAACTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGa < 1:54876/53‑1 (MQ=255) ggTGGACGTAAAAACTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGa < 1:3337365/53‑1 (MQ=255) cGTAAAAATTGCGCCAAAGATCCGGTCTATCGCAAGATGGAAAGCGatat < 1:2669695/50‑1 (MQ=38) cGTAAAAATTGCGCCAAAGATCCGGTCTATCGCAAGATGGAAAGCGatat < 1:797179/50‑1 (MQ=38) cGTAAAAATTGCGCCAAAGATCCGGTCTATCGCAAGATGGAAAGCGatat < 1:943526/50‑1 (MQ=38) cGTAAAAATTGCGCCAAAGATCCGGTCTATCGCAAGATGGAAAGCGatat < 1:577468/50‑1 (MQ=38) cGTAAAAATTGCGCCAAAGATCCGGTCTATCGCAAGATGGAAAGCGatat < 1:1349896/50‑1 (MQ=38) cGTAAAAATTGCGCCAAAGATCCGGTCTATCGCAAGATGGAAAGCGatat < 1:1366765/50‑1 (MQ=38) cGTAAAAATTGCGCCAAAGATCCGGTCTATCGCAAGATGGAAAGCGatat < 1:1677092/50‑1 (MQ=38) cGTAAAAATTGCGCCAAAGATCCGGTCTATCGCAAGATGGAAAGCGatat < 1:1889839/50‑1 (MQ=38) cGTAAAAATTGCGCCAAAGATCCGGTCTATCGCAAGATGGAAAGCGatat < 1:2003168/50‑1 (MQ=38) cGTAAAAATTGCGCCAAAGATCCGGTCTATCGCAAGATGGAAAGCGatat < 1:2013868/50‑1 (MQ=38) cGTAAAAATTGCGCCAAAGATCCGGTCTATCGCAAGATGGAAAGCGatat < 1:2062728/50‑1 (MQ=38) gTAAAAATTGCGCCAAAGATCCGGTCTATCGCAAGATGGAAAGCGatat < 1:3160122/49‑1 (MQ=38) gTAAAAATTGCGCCAAAGATCCGGTCTATCGCAAGATGGAAAGCGatat < 1:2775780/49‑1 (MQ=38) aaaaCTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGATATGCATa < 1:324234/51‑1 (MQ=255) aaaaCTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGATATGCATa < 1:1056137/51‑1 (MQ=255) aaaaCTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGATATGCATa < 1:3252523/51‑1 (MQ=255) aaaaCTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGATATGCATa < 1:2761177/51‑1 (MQ=255) aaaaCTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGATATGCATa < 1:2773752/51‑1 (MQ=255) aaaaCTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGATATGCATa < 1:3157555/51‑1 (MQ=255) aaaaCTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGATATGCATAACCTGCAGCCGTCAGTCg > 1:1027730/1‑69 (MQ=255) aaaaCTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGATATGCATAACCTGCAGCCGTCAGTCg > 1:2008063/1‑69 (MQ=255) aaaaCTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGATATGCATAACCTGCAGCCGTCAGTCg > 1:1188707/1‑69 (MQ=255) aaaaCTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGATATGCATAACCTGCAGCCGTCAGTCg > 1:128868/1‑69 (MQ=255) aCTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGATATGCATAAcc > 1:2557516/1‑51 (MQ=255) cTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGATATGCATAACCTGCAGCCGTCAGTCggtga < 1:3405892/69‑1 (MQ=255) cTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGATATGCATAACCTGCAGCCGTCAGTCggtga > 1:1506857/1‑69 (MQ=255) cTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGATATGCATAACCTGCAGCCGTCAGTCggtga < 1:1625294/69‑1 (MQ=255) cTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGATATGCATAACCTGCAGCCGTCAGTCggtga < 1:1084660/69‑1 (MQ=255) cTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGATATGCATAACCTGCAGCCGTCAGTCggtga < 1:873439/69‑1 (MQ=255) cTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGATATGCATAACCTGCAGCCGTCAGTCggtga > 1:1082361/1‑69 (MQ=255) cTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGATATGCATAACCTGCAGCCGTCAGTCggtga < 1:2685442/69‑1 (MQ=255) cTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGATATGCATAACCTGCAGCCGTCAGTCggtga < 1:2446091/69‑1 (MQ=255) | TCGTTCCCGCCTGGCAGTTCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAACTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGATATGCATAACCTGCAGCCGTCAGTCGGTGA > minE/1977594‑1977723 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |