| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,977,661 | T→C | 37.3% | A107A (GCT→GCC) | endA → | DNA‑specific endonuclease I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,977,661 | 0 | T | C | 37.3% | 102.4 / 114.5 | 174 | A107A (GCT→GCC) | endA | DNA‑specific endonuclease I |
| Reads supporting (aligned to +/- strand): ref base T (48/61); new base C (12/53); total (60/114) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.51e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01 | |||||||||||
Only 100 of 174 total aligned reads displayed.
TCCCGCCTGGCAGTTCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAACTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGATATGCATAACCTGCAGCCGTCAGTCGGTGAG > minE/1977598‑1977724 | tCCTGCCTGGCAATTCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAATTGCGCCAAAg < 1:2190982/68‑1 (MQ=255)tCCTGCCTGGCAATTCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAATTGCGCCAAAg < 1:1016460/68‑1 (MQ=255)tCCTGCCTGGCAATTCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAATTGCGCCAAAg < 1:2253654/68‑1 (MQ=255)tCCTGCCTGGCAATTCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAATTGCGCCAAAg < 1:1299929/68‑1 (MQ=255)tCCTGCCTGGCAATTCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAATTGCGCCAAAg < 1:212477/68‑1 (MQ=255)tCCCGCCTGGCAGTTCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAACTGCGCTAAAg < 1:3360546/68‑1 (MQ=255) cctgCCTGGCAATTCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAATTGCGCCAAAGAt > 1:3279709/4‑69 (MQ=255) cctgCCTGGCAATTCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAATTGCGCCAAAGAt > 1:393844/4‑69 (MQ=255) cctgCCTGGCAATTCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAATTGCGCCAAAGAt > 1:2275601/4‑69 (MQ=255) cctgCCTGGCAATTCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAATTGCGCCAAAGAt > 1:1347218/4‑69 (MQ=255) cctgCCTGGCAATTCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAATTGCGCCAAAGAt > 1:234160/4‑69 (MQ=255) cctgCCTGGCAATTCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAATTGCGCCAAAGAt > 1:2287174/4‑69 (MQ=255) cccGCCTGGCAGTTCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAACTGCGCTAAAGAt > 1:2562089/1‑69 (MQ=255) cccGCCTGGCAGTTCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAACTGCGCTAAAGAt > 1:3075075/1‑69 (MQ=255) cccGCCTGGCAGTTCGGTCACCAGCGCCAGTGCTGGCAGGA‑GGTGGACGTAAAAACTGCGCTAAAGATc > 1:56857/1‑69 (MQ=255) tCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAATTGCGCCAAAGATc < 1:170920/57‑1 (MQ=255) tCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAATTGCGCCAAAGATc < 1:3110976/57‑1 (MQ=255) tCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAATTGCGCCAAAGATc < 1:2502014/57‑1 (MQ=255) tCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAATTGCGCCAAAGATc < 1:2621409/57‑1 (MQ=255) tCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAATTGCGCCAAAGATc < 1:2752223/57‑1 (MQ=255) tCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAATTGCGCCAAAGATc < 1:777226/57‑1 (MQ=255) tCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAATTGCGCCAAAGATc < 1:1385774/57‑1 (MQ=255) tCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAATTGCGCCAAAGATc < 1:1247536/57‑1 (MQ=255) cGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAACTGCGCTAAAGATCCGGTCTAt > 1:740173/1‑64 (MQ=255) cGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAACTGCGCTAAAGATCCGGTCTATc > 1:2986777/1‑65 (MQ=255) cGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAACTGCGCTAAAGATCCGGTCTATc > 1:1845442/1‑65 (MQ=255) cGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAACTGCGCTAAAGATCCGGTCTATc > 1:1948746/1‑65 (MQ=255) cGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAACTGCGCTAAAGATCCGGTCTATc > 1:2641147/1‑65 (MQ=255) cGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAACTGCGCTAAAGATCCGGTCAATc > 1:2799235/1‑65 (MQ=255) cAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAACTGCGCTAAAGATCCGGTCTATCGCAAGATGGaa < 1:3176150/69‑1 (MQ=255) cAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAACTGCGCTAAAGATCCGGTCTATCGCAAGATGGaa > 1:2894710/1‑69 (MQ=255) cAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAACTGCGCTAAAGATCCGGTCTATCGCAAGATGGaa > 1:833108/1‑69 (MQ=255) cAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAACTGCGCTAAAGATCCGGTCTATCGCAAGATGGa < 1:1767217/68‑1 (MQ=255) cAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAACTGCGCTAAAGATCCGGTCTATCGCAAGATGGa < 1:2462552/68‑1 (MQ=255) aGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAATTGCGCCAAAGATc < 1:2202912/48‑1 (MQ=39) ccAGTGCTGGCAGGACGGTGGACGTAAAAACTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGa < 1:925781/69‑1 (MQ=255) ccAGTGCTGGCAGGACGGTGGACGTAAAAACTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGa < 1:2504773/69‑1 (MQ=255) ccAGTGCTGGCAGGACGGTGGACGTAAAAACTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGa < 1:1649485/69‑1 (MQ=255) ccAGTGCTGGCAGGACGGTGGACGTAAAAACTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGa < 1:966673/69‑1 (MQ=255) ccAGTGCTGGCAGGACGGTGGACGTAAAAACTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGa < 1:1612419/69‑1 (MQ=255) cAGTGCTGGCAGGACGGTGGACGTAAAAACTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGat < 1:1362580/69‑1 (MQ=255) gCTGGCAGGACGGTGGACGTAAAAACTGCGCTAAAg > 1:1726278/1‑36 (MQ=255) cTGGCAGGACGGTGGACGTAAAAACTGCGCTAAAGATCCGGTCt > 1:1485080/1‑44 (MQ=255) cTGGCAGGACGGTGGACGTAAAAACTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGATATGc > 1:64577/1‑68 (MQ=255) cTGGCAGGACGGTGGACGTAAAAACTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGATATGc > 1:2955833/1‑68 (MQ=255) cTGGCAGGACGGTGGACGTAAAAACTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGATATGc > 1:199950/1‑68 (MQ=255) cTGGCAGGACGGTGGACGTAAAAACTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGATATGc > 1:1142324/1‑68 (MQ=255) ggCAGGACGGTGGACGTAAAAACTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGATATGCATa < 1:1386682/69‑1 (MQ=255) ggCAGGACGGTGGACGTAAAAACTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGATATGCATa < 1:3363280/69‑1 (MQ=255) ggCAGGACGGTGGACGTAAAAACTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGATATGCATa < 1:151785/69‑1 (MQ=255) gCAGGACGGTGGACGTAAAAACTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGATATGCATa < 1:2856732/68‑1 (MQ=255) gggtggtCGTAAAATCTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCgt < 1:710054/53‑2 (MQ=255) ggTGGACGTAAAAACTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAATCGa > 1:1451849/1‑53 (MQ=255) ggTGGACGTAAAAACTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGa < 1:3425322/53‑1 (MQ=255) ggTGGACGTAAAAACTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGa < 1:690662/53‑1 (MQ=255) ggTGGACGTAAAAACTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGa < 1:2282314/53‑1 (MQ=255) ggTGGACGTAAAAACTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGa < 1:3262266/53‑1 (MQ=255) ggTGGACGTAAAAACTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGa < 1:2853439/53‑1 (MQ=255) ggTGGACGTAAAAACTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGa > 1:352012/1‑53 (MQ=255) ggTGGACGTAAAAACTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGa < 1:54876/53‑1 (MQ=255) tGGACGTAAAAACTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGa < 1:374649/51‑1 (MQ=255) cGTAAAAATTGCGCCAAAGATCCGGTCTATCGCAAGATGGAAAGCGatat < 1:577468/50‑1 (MQ=38) cGTAAAAATTGCGCCAAAGATCCGGTCTATCGCAAGATGGAAAGCGatat < 1:943526/50‑1 (MQ=38) cGTAAAAATTGCGCCAAAGATCCGGTCTATCGCAAGATGGAAAGCGatat < 1:3240102/50‑1 (MQ=38) cGTAAAAATTGCGCCAAAGATCCGGTCTATCGCAAGATGGAAAGCGatat < 1:797179/50‑1 (MQ=38) cGTAAAAATTGCGCCAAAGATCCGGTCTATCGCAAGATGGAAAGCGatat < 1:53134/50‑1 (MQ=38) cGTAAAAATTGCGCCAAAGATCCGGTCTATCGCAAGATGGAAAGCGatat < 1:2669695/50‑1 (MQ=38) cGTAAAAATTGCGCCAAAGATCCGGTCTATCGCAAGATGGAAAGCGatat < 1:2971750/50‑1 (MQ=38) cGTAAAAATTGCGCCAAAGATCCGGTCTATCGCAAGATGGAAAGCGatat < 1:1403409/50‑1 (MQ=38) cGTAAAAATTGCGCCAAAGATCCGGTCTATCGCAAGATGGAAAGCGatat < 1:1677092/50‑1 (MQ=38) cGTAAAAATTGCGCCAAAGATCCGGTCTATCGCAAGATGGAAAGCGatat < 1:1889839/50‑1 (MQ=38) cGTAAAAATTGCGCCAAAGATCCGGTCTATCGCAAGATGGAAAGCGatat < 1:2061628/50‑1 (MQ=38) gTAAAAATTGCGCCAAAGATCCGGTCTATCGCAAGATGGAAAGCGatat < 1:2775780/49‑1 (MQ=38) aaaaCTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGATATGCATa < 1:3157555/51‑1 (MQ=255) aaaaCTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGATATGCATa < 1:324234/51‑1 (MQ=255) aaaaCTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGATATGCATa < 1:1056137/51‑1 (MQ=255) aaaaCTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGATATGCATa < 1:2773752/51‑1 (MQ=255) aaaaCTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGATATGCATa < 1:2761177/51‑1 (MQ=255) aaaaCTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGATATGCATa < 1:3371602/51‑1 (MQ=255) aaaaCTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGATATGCATAACCTGCAGCCGTCAGTCg > 1:1027730/1‑69 (MQ=255) aaaaCTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGATATGCATAACCTGCAGCCGTCAGTCg > 1:149088/1‑69 (MQ=255) aaaaCTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGATATGCATAACCTGCAGCCGTCAGTCg > 1:2008063/1‑69 (MQ=255) aaaaCTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGATATGCATAACCTGCAGCCGTCAGTCg > 1:2219334/1‑69 (MQ=255) aCTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGATATGCATAAcc > 1:2557516/1‑51 (MQ=255) cTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGATATGCATAACCTGCAGCCGTCAGTc > 1:338694/1‑64 (MQ=255) cTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGATATGCATAACCTGCAGCCGTCAGTCggtga > 1:1506857/1‑69 (MQ=255) cTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGATATGCATAACCTGCAGCCGTCAGTCggtga < 1:873439/69‑1 (MQ=255) cTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGATATGCATAACCTGCAGCCGTCAGTCggtga > 1:1082361/1‑69 (MQ=255) cTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGATATGCATAACCTGCAGCCGTCAGTCggtga < 1:1303868/69‑1 (MQ=255) cTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGATATGCATAACCTGCAGCCGTCAGTCggtga < 1:3405892/69‑1 (MQ=255) cTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGATATGCATAACCTGCAGCCGTCAGTCggtga > 1:46979/1‑69 (MQ=255) cTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGATATGCATAACCTGCAGCCGTCAGTCggtga < 1:1622531/69‑1 (MQ=255) cTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGATATGCATAACCTGCAGCCGTCAGTCggtga < 1:2685442/69‑1 (MQ=255) cTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGATATGCATAACCTGCAGCCGTCAGTCggtga < 1:2004816/69‑1 (MQ=255) cTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGATATGCATAACCTGCAGCCGTCAGTCggtga < 1:551398/69‑1 (MQ=255) cTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGATATGCATAACCTGCAGCCGTCAGTCggtga < 1:2446091/69‑1 (MQ=255) tGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGATATGCATa > 1:640856/1‑46 (MQ=255) tGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGATATGCATAACCTGCAGCCGTCAGTCggtgag > 1:1068386/1‑69 (MQ=255) tGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGATATGCATAACCTGCAGCCGTCAGTCggtgag > 1:2907667/1‑69 (MQ=255) tGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGATATGCATAACCTGCAGCCGTCAGTCggtgag > 1:3033827/1‑69 (MQ=255) | TCCCGCCTGGCAGTTCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAACTGCGCTAAAGATCCGGTCTATCGCAAGATGGAAAGCGATATGCATAACCTGCAGCCGTCAGTCGGTGAG > minE/1977598‑1977724 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |