| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,980,802 | C→A | 50.6% | A103A (GCC→GCA) | yqgF → | predicted Holliday junction resolvase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,980,802 | 0 | C | A | 50.6% | 5.5 / 90.4 | 71 | A103A (GCC→GCA) | yqgF | predicted Holliday junction resolvase |
| Reads supporting (aligned to +/- strand): ref base C (29/6); new base A (16/20); total (45/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.20e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.82e-01 | |||||||||||
GTTTCGGTGTTGAAGTAAAGCTCCATGACGAGCGTCTTAGCACTGTGGAAGCCCGTTCCGGTCTGTTTGAACAGGGCGGCTATCGGGCGCTCAACAAAGGCAAAGTTGACTCT > minE/1980750‑1980862 | gTTTCGGTGTTGAAGTAAAGCTCCATGACGAGCGTCTTAGCACCGTGGAGGCACGTTCCGGTCTGTTTg < 1:1070048/69‑1 (MQ=255)gTTTCGGTGTTGAAGTAAAGCTCCATGACGAGCGTCTTAGCACCGTGGAGGCACGTTCCGGTCTGTTTg < 1:1821940/69‑1 (MQ=255)gTTTCGGTGTTGAAGTAAAGCTCCATGACGAGCGTCTTAGCACCGTGGAGGCACGTTCCGGTCTGTTTg < 1:204000/69‑1 (MQ=255)gTTTCGGTGTTGAAGTAAAGCTCCATGACGAGCGTCTTAGCACCGTGGAGGCACGTTCCGGTCTGTTTg < 1:356246/69‑1 (MQ=255)gTTTCGGTGTTGAAGTAAAGCTCCATGACGAGCGTCTTAGCACCGTGGAGGCACGTTCCGGTCTGTTTg < 1:2352614/69‑1 (MQ=255)gTTTCGGTGTTGAAGTAAAGCTCCATGACGAGCGTCTTAGCACCGTGGAGGCACGTTCCGGTCTGTTTg < 1:2368813/69‑1 (MQ=255)gTTTCGGTGTTGAAGTAAAGCTCCATGACGAGCGTCTTAGCACCGTGGAGGCACGTTCCGGTCTGTTTg < 1:2733771/69‑1 (MQ=255)gTTTCGGTGTTGAAGTAAAGCTCCATGACGAGCGTCTTAGCACCGTGGAGGCACGTTCCGGTCTGTTTg < 1:2801677/69‑1 (MQ=255)gTTTCGGTGTTGAAGTAAAGCTCCATGACGAGCGTCTTAGCACCCTGGAGGCACGTTCCGGTCTGTTTg < 1:1211546/69‑1 (MQ=255) tCGGTGTTGAAGTAAAGCTCCATGACGAGCGTCTTAGCACTGTGGAAGCCCGTTCCGGTCTGTTTGAAc > 1:2043663/1‑69 (MQ=255) tCGGTGTTGAAGTAAAGCTCCATGACGAGCGTCTTAGCACTGTGGAAGCCCGTTCCGGTCTGTTTGAAc > 1:448685/1‑69 (MQ=255) tCGGTGTTGAAGTAAAGCTCCATGACGAGCGTCTTAGCACCGTGGAGGCACGTTcc > 1:507221/1‑56 (MQ=255) tCGGTGTTGAAGTAAAGCTCCATGACGAGCGTCTTAGCACCGTGGAGGCACGTTCCg > 1:3313173/1‑57 (MQ=255) tCGGTGTTGAAGTAAAGCTCCATGACGAGCGTCTTAGCACCGTGGAGGCACGTTCCg > 1:252705/1‑57 (MQ=255) tCGGTGTTGAAGTAAAGCTCCATGACGAGCGTCTTAGCACCGTGGAGGCACGTTCCg > 1:3234851/1‑57 (MQ=255) tCGGTGTTGAAGTAAAGCTCCATGACGAGCGTCTTAGCACCGTGGAGGCACGTTCCg > 1:2264464/1‑57 (MQ=255) tCGGTGTTGAAGTAAAGCTCCATGACGAGCGTCTTAGCACCGTGGAGGCACGTTCCg > 1:215060/1‑57 (MQ=255) tCGGTGTTGAAGTAAAGCTCCATGACGAGCGTCTTAGCACCGTGGAGGCACGTTCCg > 1:3128992/1‑57 (MQ=255) tCGGTGTTGAAGTAAAGCTCCATGACGAGCGTCTTAGCACCGTGGAGGCACGTTCCg > 1:2034083/1‑57 (MQ=255) tCGGTGTTGAAGTAAAGCTCCATGACGAGCGTCTTAGCACCGTGGAGGCACGTTCCg > 1:30019/1‑57 (MQ=255) tCGGTGTTGAAGTAAAGCTCCATGACGAGCGTCTTAGCACCGTGGAGGCACGTTCCg > 1:40150/1‑57 (MQ=255) tCGGTGTTGAAGTAAAGCTCCATGACGAGCGTCTTAGCACCGTGGAGGCACGTTCCg > 1:1861287/1‑57 (MQ=255) tCGGTGTTGAAGTAAAGCTCCATGACGAGCGTCTTAGCACCGTGGAGGCACGTTCCg > 1:1757821/1‑57 (MQ=255) tCGGTGTTGAAGTAAAGCTCCATGACGAGCGTCTTAGCACCGTGGAGGCACGTTCCg > 1:114192/1‑57 (MQ=255) tCGGTGTTGAAGTAAAGCTCCATGACGAGCGTCTTAGCACCGTGGAGGCACGTTCCg > 1:1144864/1‑57 (MQ=255) tCGGTGTTGAAGTAAAGCTCCATGACGAGCGTCTTAGCACCGTGGAGGCACGTTCCg > 1:1170746/1‑57 (MQ=255) tCGGTGTTGAAGTAAAGCTCCATGACGAGCGTCTTAGCACCGTGGAGGCACGTTCCg > 1:1961081/1‑57 (MQ=255) tCGGTGTTGAAGTAAAGCTCCATGACGAGCGTCTTAGCACCGTGGAGGCACGTTCCGGTCTGTTTg < 1:3118231/66‑1 (MQ=255) aaaGCTCCATGACGAGCGTCTTAGCACTGTGGAAGCCCGTTCCGGTCTGTTTGAACAGGGCGGCTATc > 1:1436135/1‑68 (MQ=255) aaaGCTCCATGACGAGCGTCTTAGCACTGTGGAAGCCCGTTCCGGTCTGTTTGAACAGGGCGGCTATCg > 1:1999645/1‑69 (MQ=255) aaaGCTCCATGACGAGCGTCTTAGCACTGTGGAAGCCCGTTCCGGTCTGTTTGAACAGGGCGGCTATCg > 1:1772531/1‑69 (MQ=255) aaaGCTCCATGACGAGCGTCTTAGCACTGTGGAAGCCCGTTCCGGTCTGTTTGAACAGGGCGGCTATCg > 1:2697350/1‑69 (MQ=255) aaaGCTCCATGACGAGCGTCTTAGCACCGTGGAGGCACGTTCCGGTCTGTTTGAACAGGGCGGCTATCg < 1:817833/69‑1 (MQ=255) aaaGCTCCATGACGAGCGTCTTAGCACCGTGGAGGCACGTTCCGGTCTGTTTGAACAGGGCGGCTATCg < 1:1258076/69‑1 (MQ=255) aaaGCTCCATGACGAGCGTCTTAGCACCGTGGAGGCACGTTCCGGTCTGTTTGAACAGGGCGGCTATCg < 1:2607908/69‑1 (MQ=255) aaaGCTCCATGACGAGCGTCTTAGCACCGTGGAGGCACGTTCCGGTCTGTTTGAACAGGGCGGCTATCg < 1:1380186/69‑1 (MQ=255) aaaGCTCCATGACGAGCGTCTTAGCACCGTGGAGGCACGTTCCGGTCTGTTTGAACAGGGCGGCTATCg < 1:2300372/69‑1 (MQ=255) aaaGCTCCATGACGAGCGTCTTAGCACCGTGGAGGCACGTTCCGGTCTGTTTGAACAGGGCGGCTATCg < 1:2192745/69‑1 (MQ=255) aaaGCTCCATGACGAGCGTCTTAGCACCGTGGAGGCACGTTCCGGTCTGTTTGAACAGGGCGGCTATCg < 1:1538093/69‑1 (MQ=255) aaaGCTCCATGACGAGCGTCTTAGCACCGTGGAGGCACGTTCCGGTCTGTTTGAACAGGGCGGCTATCg < 1:2123833/69‑1 (MQ=255) aaaGCTCCATGACGAGCGTCTTAGCACCGTGGAGGCACGTTCCGGTCTGTTTGAACAGGGCGGCTATCg < 1:607001/69‑1 (MQ=255) aaGCTCCATGACGAGCGTCTTAGCACTGTGGAAGCCCGTTCCGGTCTGTTTGAACAGGGCGGCTATCgg > 1:833434/1‑69 (MQ=255) aaGCTCCATGACGAGCGTCTTAGCACTGTGGAAGCCCGTTCCGGTCTGTTTGAACAGGGCGGCTATCgg > 1:3351242/1‑69 (MQ=255) aaGCTCCATGACGAGCGTCTTAGCACTGTGGAAGCCCGTTCCGGTCTGTTTGAACAGGGCGGCTATCgg > 1:876159/1‑69 (MQ=255) aaGCTCCATGACGAGCGTCTTAGCACTGTGGAAGCCCGTTCCGGTCTGTTTGAACAGGGCGGCTATCgg > 1:683999/1‑69 (MQ=255) aaGCTCCATGACGAGCGTCTTAGCACTGTGGAAGCCCGTTCCGGTCTGTTTGAACAGGGCGGCTATCgg > 1:1071063/1‑69 (MQ=255) aaGCTCCATGACGAGCGTCTTAGCACTGTGGAAGCCCGTTCCGGTCTGTTTGAACAGGGCGGCTATCgg > 1:2843291/1‑69 (MQ=255) aaGCTCCATGACGAGCGTCTTAGCACTGTGGAAGCCCGTTCCGGTCTGTTTGAACAGGGCGGCTATCgg > 1:1801789/1‑69 (MQ=255) aaGCTCCATGACGAGCGTCTTAGCACTGTGGAAGCCCGTTCCGGTCTGTTTGAACAGGGCGGCTATCgg > 1:2176445/1‑69 (MQ=255) aaGCTCCATGACGAGCGTCTTAGCACTGTGGAAGCCCGTTCCGGTCTGTTTGAACAGGGCGGCTATCgg > 1:2618930/1‑69 (MQ=255) aaGCTCCATGACGAGCGTCTTAGCACTGTGGAAGCCCGTTCCGGTCTGTTTGAACAGGGCGGCTATCgg > 1:2623164/1‑69 (MQ=255) aaGCTCCATGACGAGCGTCTTAGCACTGTGGAAGCCCGTTCCGGTCTGTTTGAACAGGGCGGCTATCg > 1:3399457/1‑68 (MQ=255) ccATGACGAGCGTCTTAGCACTGTGGAAGCCCGTTCCGGTCTGTTTGAACAGGGCGGCTATCGGgcgc > 1:2030533/1‑68 (MQ=255) ccATGACGAGCGTCTTAGCACTGTGGAAGCCCGTTCCGGTCTGTTTGAACAGGGCGGCTATCGGgcgc > 1:43384/1‑68 (MQ=255) ttAGCACTGTGGAAGCCCGTTGCGGTCTGTTTGAACAGGGCGGCTATCGGGCGCTCAACAAAGGCAAAg > 1:3346685/1‑69 (MQ=255) ttAGCACTGTGGAAGCCCGTTCCGGTCTGTTTGAACAGGGCGGCTATCGGGCGCTCAACAAAGGCAAAg > 1:3215472/1‑69 (MQ=255) ttAGCACTGTGGAAGCCCGTTCCGGTCTGTTTGAACAGGGCGGCTATCGGGCGCTCAACAAAGGCAAAg > 1:1944392/1‑69 (MQ=255) ttAGCACTGTGGAAGCCCGTTCCGGTCTGTTTGAACAGGGCGGCTATCGGGCGCTCAACAAAGGCAAAg > 1:796412/1‑69 (MQ=255) ttAGCACTGTGGAAGCCCGTTCCGGTCTGTTTGAACAGGGCGGCTATCGGGCGCTCAACAAAGGCAAAg > 1:853701/1‑69 (MQ=255) aCTGTGGAAGCCCGTTCCGGTCTGTTTGAACAGGGCGGCTATCGGGCGCTCAACAAAGGCAAAGTTGa < 1:3293470/68‑1 (MQ=255) gtgGAAGCCCGTTCCGGTCTGTTTGAACAGGGCGGCTATCGGGCGCTCAACAAAGGCAAAGTTGActct > 1:2981063/1‑69 (MQ=255) gtgGAAGCCCGTTCCGGTCTGTTTGAACAGGGCGGCTATCGGGCGCTCAACAAAGGCAAAGTTGActct > 1:1205892/1‑69 (MQ=255) gtgGAAGCCCGTTCCGGTCTGTTTGAACAGGGCGGCTATCGGGCGCTCAACAAAGGCAAAGTTGActct > 1:93313/1‑69 (MQ=255) gtgGAAGCCCGTTCCGGTCTGTTTGAACAGGGCGGCTATCGGGCGCTCAACAAAGGCAAAGTTGActct < 1:874713/69‑1 (MQ=255) gtgGAAGCCCGTTCCGGTCTGTTTGAACAGGGCGGCTATCGGGCGCTCAACAAAGGCAAAGTTGActct < 1:866591/69‑1 (MQ=255) gtgGAAGCCCGTTCCGGTCTGTTTGAACAGGGCGGCTATCGGGCGCTCAACAAAGGCAAAGTTGActct < 1:1370335/69‑1 (MQ=255) gtgGAAGCCCGTTCCGGTCTGTTTGAACAGGGCGGCTATCGGGCGCTCAACAAAGGCAAAGTTGActct > 1:140959/1‑69 (MQ=255) gtgGAAGCCCGTTCCGGTCTGTTTGAACAGGGCGGCTATCGGGCGCTCAACAAAGGCAAAGTTGActct < 1:1775332/69‑1 (MQ=255) gtgGAAGCCCGTTCCGGTCTGTTTGAACAGGGCGGCTATCGGGCGCTCAACAAAGGCAAAGTTGActct > 1:44527/1‑69 (MQ=255) gtgGAAGCCCGTTCCGGTCTGTTTGAACAGGGCGGCTATCGGGCGCTCAACAAAGGCAAAGTTGActct < 1:2619819/69‑1 (MQ=255) ggAGGCACGTTCCGGTCTGTTTGAACAGGGCGGCTATCg < 1:1439409/39‑1 (MQ=38) | GTTTCGGTGTTGAAGTAAAGCTCCATGACGAGCGTCTTAGCACTGTGGAAGCCCGTTCCGGTCTGTTTGAACAGGGCGGCTATCGGGCGCTCAACAAAGGCAAAGTTGACTCT > minE/1980750‑1980862 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |