Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	1,981,950	G→A	39.9%	P42L (CCG→CTG)	yggR ←	predicted transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,981,950	0	G	A	39.9%	20.9 / 31.3	50	P42L (CCG→CTG)	yggR	predicted transporter
Reads supporting (aligned to +/- strand):  ref base G (16/14);  new base A (11/9);  total (27/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01

CTCCAGCAATATTGCCCGCTGATCGTCATCCAGCCACTCCCGCAGTAGCTCTTCGACGTCCGGCGTATCAAACGGCGCAGCTTCCATTCTGCCGCGAATGCGCCATCGTGCGGGCCAGGCGCTG  >  minE/1981889‑1982012                                                              |                                                               ctcCAGCAATATTGCCCGCTTATCGTCATCCAGCCACTCCCGCAGTAGCTCTTCGATGTCCGGCGTAt                                                          >  1:376660/1‑68 (MQ=255) ctcCAGCAATATTGCCCGCTGATCGTCATCCAGCCACTCCCGCAGTAGCTCTTCGACGTCCGGCGTa                                                           >  1:1618404/1‑67 (MQ=255) ctcCAGCAATATTGCCCGCTGATCGTCATCCAGCCACTCCCGCAGTAGCTCTTCGACGTCCGGCGTAt                                                          >  1:2762941/1‑68 (MQ=255)     aGCAATATTGTCCGCTGATCGTCATCCAGCCACTCCCGCAGTAGCTCTTCGACGTCCAGCGCATCAAAc                                                     >  1:2655661/1‑69 (MQ=255)     aGCAATATTGTCCGCTGATCGTCATCCAGCCACTCCCGCAGTAGCTCTTCGACGTCCAGCGCATCAAAc                                                     >  1:982912/1‑69 (MQ=255)     aGCAATATTGTCCGCTGATCGTCATCCAGCCACTCCCGCAGTAGCTCTTCGACGTCCAGCGCATCAAAc                                                     >  1:77463/1‑69 (MQ=255)     aGCAATATTGTCCGCTGATCGTCATCCAGCCACTCCCGCAGTAGCTCTTCGACGTCCAGCGCATCAAAc                                                     >  1:3082087/1‑69 (MQ=255)     aGCAATATTGTCCGCTGATCGTCATCCAGCCACTCCCGCAGTAGCTCTTCGACGTCCAGCGCATCAAAc                                                     >  1:2889780/1‑69 (MQ=255)     aGCAATATTGTCCGCTGATCGTCATCCAGCCACTCCCGCAGTAGCTCTTCGACGTCCAGCGCATCAAAc                                                     >  1:1046925/1‑69 (MQ=255)     aGCAATATTGTCCGCTGATCGTCATCCAGCCACTCCCGCAGTAGCTCTTCGACGTCCAGCGCATCAAAc                                                     >  1:2389312/1‑69 (MQ=255)     aGCAATATTGTCCGCTGATCGTCATCCAGCCACTCCCGCAGTAGCTCTTCGACGTCCAGCGCATCAAAc                                                     >  1:2168160/1‑69 (MQ=255)     aGCAATATTGTCCGCTGATCGTCATCCAGCCACTCCCGCAGTAGCTCTTCGACGTCCAGCGCATCAAAc                                                     >  1:2056264/1‑69 (MQ=255)     aGCAATATTGTCCGCTGATCGTCATCCAGCCACTCCCGCAGTAGCTCTTCGACGTCCAGCGCATCAAAc                                                     >  1:2050006/1‑69 (MQ=255)     aGCAATATTGTCCGCTGATCGTCATCCAGCCACTCCCGCAGTAGCTCTTCGACGTCCAGCGCATCAAAc                                                     >  1:1756509/1‑69 (MQ=255)           atTGCCCGCTGATCGTCATCCAGCCACTCCCGCAGTAGCTCTTCGACGTCCGGCGTATCAAACGGCGCa                                               >  1:906659/1‑69 (MQ=255)           atTGCCCGCTGATCGTCATCCAGCCACTCCCGCAGTAGCTCTTCGACGTCCGGCGTATCAAACGGCGCa                                               >  1:1375543/1‑69 (MQ=255)            ttGCCCGCTGATCGTCATCCAGCCACTCCCGCAGTAGCTCTTCGACGTCCGGCGTATCAAACGgcgc                                                >  1:513583/1‑67 (MQ=255)            ttGCCCGCTGATCGTCATCCAGCCACTCCCGCAGTAGCTCTTCGACGTCCGGCGTATCAAACGGCGCAg                                              >  1:3155912/1‑69 (MQ=255)            ttGCCCGCTGATCGTCATCCAGCCACTCCCGCAGTAGCTCTTCGACGTCCGGCGTATCAAACGGCGCAg                                              >  1:1478514/1‑69 (MQ=255)            ttGCCCGCTGATCGTCATCCAGCCACTCCCGCAGTAGCTCTTCGACGTCCGGCGTATCAAACGGCGCAg                                              >  1:769989/1‑69 (MQ=255)            ttGCCCGCTGATCGTCATCCAGCCACTCCCGCAGTAGCTCTTCGACGTCCGGCGTATCAAACGGCGCAg                                              >  1:1941692/1‑69 (MQ=255)                        cGTCATCCAGCCACTCCCGCAGTAGCTCTTCGACGTCCGGCGTATCAAACGGCGCAGCTTCCATTCTGc                                  >  1:2486326/1‑69 (MQ=255)                        cGTCATCCAGCCACTCCCGCAGTAGCTCTTCGACGTCCGGCGTATCAAACGGCGCAGCTTCCATTCTGc                                  >  1:1263256/1‑69 (MQ=255)                        cGTCATCCAGCCACTCCCGCAGTAGCTCTTCGACGTCCGGCGTATCAAACGGCGCAGCTTCCATTCTGc                                  >  1:3076851/1‑69 (MQ=255)                                 gCCACTCCCGCAGTAGCTCTTCGACGTCCAGCGCATCAAACGGCGCAGCTTcc                                         <  1:163945/53‑1 (MQ=255)                                 gCCACTCCCGCAGTAGCTCTTCGACGTCCAGCGCATCAAACGGCGCAGCTTcc                                         <  1:531905/53‑1 (MQ=255)                                 gCCACTCCCGCAGTAGCTCTTCGACGTCCAGCGCATCAAACGGCGCAGCTTcc                                         <  1:871996/53‑1 (MQ=255)                                 gCCACTCCCGCAGTAGCTCTTCGACGTCCAGCGCATCAAACGGCGCAGCTTcc                                         <  1:1925096/53‑1 (MQ=255)                                 gCCACTCCCGCAGTAGCTCTTCGACGTCCAGCGCATCAAACGGCGCAGCTTcc                                         <  1:1094664/53‑1 (MQ=255)                                 gCCACTCCCGCAGTAGCTCTTCGACGTCCAGCGCATCAAACGGCGCAGCTTcc                                         <  1:3385991/53‑1 (MQ=255)                                 gCCACTCCCGCAGTAGCTCTTCGACGTCCAGCGCATCAAACGGCGCAGCTTcc                                         <  1:1155825/53‑1 (MQ=255)                                 gCCACTCCCGCAGTAGCTCTTCGACGTCCAGCGCATCAAACGGCGCAGCTTcc                                         <  1:3103233/53‑1 (MQ=255)                                    aCTCCCGCAGTAGCTCTTCGACGTCCAGCGCATCAAACGGCGCAGCTTcc                                         <  1:584654/50‑1 (MQ=255)                                      tCCCGCAGTAGCTCTTCGACGTCCGGCGTATCAAACGGCTCAGCTTCCATTCTGCCGCGAATGCGCCAt                    <  1:1055592/69‑1 (MQ=255)                                      tCCCGCAGTAGCTCTTCGACGTCCGGCGTATCAAACGGCGCAGCTTCCATTCTGCCGCGAATGCGCCAt                    <  1:3206256/69‑1 (MQ=255)                                      tCCCGCAGTAGCTCTTCGACGTCCGGCGTATCAAACGGCGCAGCTTCCATTCTGCCGCGAATGCGCCAt                    <  1:1357118/69‑1 (MQ=255)                                      tCCCGCAGTAGCTCTTCGACGTCCGGCGTATCAAACGGCGCAGCTTCCATTCTGCCGCGAATGCGCCAt                    <  1:503915/69‑1 (MQ=255)                                      tCCCGCAGTAGCTCTTCGACGTCCGGCGTATCAAACGGCGCAGCTTCCATTCTGCCGCGAATGCGCCAt                    <  1:2694067/69‑1 (MQ=255)                                      tCCCGCAGTAGCTCTTCGACGTCCGGCGTATCAAACGGCGCAGCTTCCATTCTGCCGCGAATGCGCCAt                    <  1:2226318/69‑1 (MQ=255)                                      tCCCGCAGTAGCTCTTCGACGTCCGGCGTATCAAACGGCGCAGCTTCCATTCTGCCGCGAATGCGCCAt                    <  1:550059/69‑1 (MQ=255)                                      tCCCGCAGTAGCTCTTCGACGTCCGGCGTATCAAACGGCGCAGCTTCCATTCTGCCGCGAATGCGCCAt                    <  1:2190508/69‑1 (MQ=255)                                      tCCCGCAGTAGCTCTTCGACGTCCGGCGTATCAAACGGCGCAGCTTCCATTCTGCCGCGAATGCGCCAt                    <  1:1677458/69‑1 (MQ=255)                                      tCCCGCAGTAGCTCTTCGACGTCCGGCGTATCAAACGGCGCAGCTTCCATTCTGCCGCGAATGCGCCAt                    <  1:899592/69‑1 (MQ=255)                                      tCCCGCAGTAGCTCTTCGACGTCCGGCGTATCAAACGGCGCAGCTTCCATTCTGCCGCGAATGCGCCAt                    <  1:181939/69‑1 (MQ=255)                                       cccGCAGTAGCTCTTCGACGTCCGGCGTATCAAACGGCGCAGCTTCCATTCTGCcgcg                              <  1:2764873/58‑1 (MQ=255)                                       cccGCAGTAGCTCTTCGACGTCCGGCGTATCAAACGGCGCAGCTTCCATTCTGCCGCGAATGCGCCATc                   >  1:2132210/1‑69 (MQ=255)                                       cccGCAGTAGCTCTTCGACGTCCGGCGTATCAAACGGCGCAGCTTCCATTCTGCCGCGAATGCGCCATc                   >  1:1085488/1‑69 (MQ=255)                                       cccGCAGTAGCTCTTCGACGTCCGGCGTATCAAACGGCGCAGCTTCCATTCTGCCGCGAATGCGCCATc                   >  1:901536/1‑69 (MQ=255)                                                         cGTCCGGCGTATCAAACGGCGCAGCTTCCATTCTGCCGCGAATGCGCCATCGTGCGGGCCAGGCGCTg  <  1:373038/68‑1 (MQ=255)                                                         cGTCCGGCGTATCAAACGGCGCAGCTTCCATTCTGCCGCGAATGCGCCATCGTGCGGGCCAGGCGCTg  <  1:1848010/68‑1 (MQ=255)                                                              |                                                               CTCCAGCAATATTGCCCGCTGATCGTCATCCAGCCACTCCCGCAGTAGCTCTTCGACGTCCGGCGTATCAAACGGCGCAGCTTCCATTCTGCCGCGAATGCGCCATCGTGCGGGCCAGGCGCTG  >  minE/1981889‑1982012

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑