| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,984,551 | T→G | 100% | T97T (ACT→ACG) | yggW → | predicted oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,984,551 | 0 | T | G | 90.4% | 67.2 / ‑0.5 | 31 | T97T (ACT→ACG) | yggW | predicted oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base T (2/1); new base G (19/9); total (21/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CGCTGCTGGACGGCGTGCGTGCGCGTTTGCCGCTGGCAGCGGATGCAGAAATTACTATGGAAGCGAACCCTGGCACGGTAGAAGCCGATCGCTTTGTCGATTATCAGCGTGCTGGTGTG > minE/1984496‑1984614 | cGCTGCTGGACGGCGTGCGTGCGCGTTTGCCGCTGGCAGCGGATGCAGAAATTACTATGGAAGCGAAcc < 1:599404/69‑1 (MQ=255) atacgtgcgCGTTTGCCGCTGGCAGCGGATGCAGAAATTACGATGGAAGCGAATCCTGGTACGGTTGa < 1:1684878/65‑1 (MQ=255) gTTTGCCGCTGGCAGCGGATGCAGAAATTACGATGGAAGCGa > 1:2564378/1‑42 (MQ=255) gTTTGCCGCTGGCAGCGGATGCAGAAATTACGATGGAAGCGAAtcc > 1:2274733/1‑46 (MQ=38) gTTTGCCGCTGGCAGCGGATGCAGAAATTACGATGGAAGCGAATCCTGGTACGGTTGAAGCCGATCGc > 1:982328/1‑68 (MQ=255) gTTTGCCGCTGGCAGCGGATGCAGAAATTACGATGGAAGCGAATCCTGGTACGGTTGAAGCCGATCGc > 1:1348131/1‑68 (MQ=255) gTTTGCCGCTGGCAGCGGATGCAGAAATTACGATGGAAGCGAATCCTGGTACGGTTGAAGCCGATCGCt > 1:3348558/1‑69 (MQ=255) gTTTGCCGCTGGCAGCGGATGCAGAAATTACGATGGAAGCGAATCCTGGTACGGTTGAAGCCGATCGCt > 1:3340718/1‑69 (MQ=255) gTTTGCCGCTGGCAGCGGATGCAGAAATTACGATGGAAGCGAATCCTGGTACGGTTGAAGCCGATCGCt > 1:2986658/1‑69 (MQ=255) gTTTGCCGCTGGCAGCGGATGCAGAAATTACGATGGAAGCGAATCCTGGTACGGTTGAAGCCGATCGCt > 1:3356916/1‑69 (MQ=255) gTTTGCCGCTGGCAGCGGATGCAGAAATTACGATGGAAGCGAATCCTGGTACGGTTGAAGCCGATCGCt > 1:380003/1‑69 (MQ=255) gTTTGCCGCTGGCAGCGGATGCAGAAATTACGATGGAAGCGAATCCTGGTACGGTTGAAGCCGATCGCt > 1:2714020/1‑69 (MQ=255) gTTTGCCGCTGGCAGCGGATGCAGAAATTACGATGGAAGCGAATCCTGGTACGGTTGAAGCCGATCGCt > 1:1058121/1‑69 (MQ=255) gTTTGCCGCTGGCAGCGGATGCAGAAATTACGATGGAAGCGAATCCTGGTACGGTTGAAGCCGATCGCt > 1:2402524/1‑69 (MQ=255) gTTTGCCGCTGGCAGCGGATGCAGAAATTACGATGGAAGCGAATCCTGGTACGGTTGAAGCCGATCGCt > 1:2363537/1‑69 (MQ=255) gTTTGCCGCTGGCAGCGGATGCAGAAATTACGATGGAAGCGAATCCTGGTACGGTTGAAGCCGATCGCt > 1:2328701/1‑69 (MQ=255) gTTTGCCGCTGGCAGCGGATGCAGAAATTACGATGGAAGCGAATCCTGGTACGGTTGAAGCCGATCGCt > 1:2197560/1‑69 (MQ=255) gTTTGCCGCTGGCAGCGGATGCAGAAATTACGATGGAAGCGAATCCTGGTACGGTTGAAGCCGATCGCt > 1:434735/1‑69 (MQ=255) gTTTGCCGCTGGCAGCGGATGCAGAAATTACGATGGAAGCGAATCCTGGTACGGTTGAAGCCGATCGCt > 1:911447/1‑69 (MQ=255) gTTTGCCGCTGGCAGCGGATGCAGAAATTACGATGGAAGCGAATCCTGGTACGGTTGAAGCCGATCGCt > 1:1433944/1‑69 (MQ=255) gTTTGCCGCTGGCAGCGGATGCAGAAATTACGATGGAAGCGAATCCTGGTACGGTTGAAGCCGATCGCt > 1:1123495/1‑69 (MQ=255) aTTACTATGGAAGCGAACCCTGGCACGGTAGAAGCCGATCGCTTTGTCGATTATCAGCGTGCTGGtgtg > 1:2688569/1‑69 (MQ=255) aTTACTATGGAAGCGAACCCTGGCACGGTAGAAGCCGATCGCTTTGTCGATTATCAGCGTGCTGGtgtg > 1:1792503/1‑69 (MQ=255) aTTACGATGGAAGCGAATCCTGGTACGGTTGAAGCCGATCGCTTTGTTGATTATCAGCGTGCTGGtgtg < 1:2937181/69‑1 (MQ=255) aTTACGATGGAAGCGAATCCTGGTACGGTTGAAGCCGATCGCTTTGTTGATTATCAGCGTGCTGGtgtg < 1:3361234/69‑1 (MQ=255) aTTACGATGGAAGCGAATCCTGGTACGGTTGAAGCCGATCGCTTTGTTGATTATCAGCGTGCTGGtgtg < 1:281897/69‑1 (MQ=255) aTTACGATGGAAGCGAATCCTGGTACGGTTGAAGCCGATCGCTTTGTTGATTATCAGCGTGCTGGtgtg < 1:584979/69‑1 (MQ=255) aTTACGATGGAAGCGAATCCTGGTACGGTTGAAGCCGATCGCTTTGTTGATTATCAGCGTGCTGGtgtg < 1:654522/69‑1 (MQ=255) aTTACGATGGAAGCGAATCCTGGTACGGTTGAAGCCGATCGCTTTGTTGATTATCAGCGTGCTGGtgtg < 1:1121181/69‑1 (MQ=255) ttACGATGGAAGCGAATCCTGGTACGGTTGAAGCCGATCGCTTTGTTGATTATCAGCGTGCTGGtgtg < 1:1782563/68‑1 (MQ=255) tACGATGGAAGCGAATCCTGGTACGGTTGAAGCCGATCGCTTTGTTGATTATCAGCGTGCTGGtgtg < 1:1998473/67‑1 (MQ=255) | CGCTGCTGGACGGCGTGCGTGCGCGTTTGCCGCTGGCAGCGGATGCAGAAATTACTATGGAAGCGAACCCTGGCACGGTAGAAGCCGATCGCTTTGTCGATTATCAGCGTGCTGGTGTG > minE/1984496‑1984614 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |