| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,985,612 | T→C | 28.6% | Q316Q (CAA→CAG) | yggM ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,985,612 | 0 | T | C | 28.6% | 102.6 / 44.1 | 101 | Q316Q (CAA→CAG) | yggM | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (53/19); new base C (13/16); total (66/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.03e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 110 total aligned reads displayed.
TATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTTCATCAAAATCGCGACTTTTTTACTGTTTATTTCTGAAACTTTCAT > minE/1985544‑1985677 | tatCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCt > 1:1559974/1‑69 (MQ=255)tatCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCt > 1:793101/1‑69 (MQ=255)tatCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCt > 1:603674/1‑69 (MQ=255)tatCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCt > 1:50300/1‑69 (MQ=255)tatCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCt > 1:1999718/1‑69 (MQ=255)tatCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCt > 1:2790491/1‑69 (MQ=255)tatCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCt > 1:2806314/1‑69 (MQ=255)tatCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCt > 1:2816816/1‑69 (MQ=255) tCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTg > 1:1049070/1‑69 (MQ=255) tCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTg > 1:1654197/1‑69 (MQ=255) tCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTg > 1:275807/1‑69 (MQ=255) tCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTg > 1:42931/1‑69 (MQ=255) aaaCTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTgg > 1:1170712/1‑68 (MQ=255) aaaCTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTgg > 1:2756532/1‑68 (MQ=255) acttaATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTgg > 1:1665282/1‑62 (MQ=255) acttaATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTg > 1:1214318/1‑65 (MQ=255) acttaATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTg > 1:921089/1‑65 (MQ=255) ttaATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATc > 1:3337103/1‑68 (MQ=255) ttaATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATc > 1:2398623/1‑68 (MQ=255) ttaATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATc > 1:467352/1‑68 (MQ=255) ttaATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATc > 1:2629873/1‑68 (MQ=255) ttaATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATc > 1:1503854/1‑68 (MQ=255) ttaATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATc > 1:134277/1‑68 (MQ=255) ccccATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAAtt > 1:1135267/1‑68 (MQ=255) cccATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCCTGGTTGA‑ATCGTCAATTTtc < 1:3365198/69‑1 (MQ=255) ccATTGCGCCGCAGTTGTTCTGTATCTTTTTTCCAGCGTTCATTAGCCTGGTTGATATCGTCAATTTtc < 1:40479/69‑1 (MQ=255) ccATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCCTGGTTGATATCGTCAATTTtc < 1:2992399/69‑1 (MQ=255) ccATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCCTGGTTGATATCGTCAATTTtc < 1:529131/69‑1 (MQ=255) ccATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCCTGGTTGATATCGTCAATTTtc < 1:2913647/69‑1 (MQ=255) ccATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCCTGGTTGATATCGTCAATTTtc < 1:2688098/69‑1 (MQ=255) ccATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCCTGGTTGATATCGTCAATTTtc < 1:213984/69‑1 (MQ=255) ccATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCCTGGTTGATATCGTCAATTTtc < 1:824262/69‑1 (MQ=255) ccATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCCTGGTTGATATCGTCAATTTtc < 1:375321/69‑1 (MQ=255) ccATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCCTGGTTGATATCGTCAATTTtc < 1:1922632/69‑1 (MQ=255) ccATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCCTGGTTGATATCGTCAATTTtc < 1:1810931/69‑1 (MQ=255) ccATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCCTGGTTGATATCGTCAATTTtc < 1:3077403/69‑1 (MQ=255) ccATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCCTGGTTGATATCGTCAATTTtc < 1:1476329/69‑1 (MQ=255) cATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCCTGGTTGATATCGTCAATTTtc < 1:1850614/68‑1 (MQ=255) cATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCCTGGTTGATATCGTCAATTTtc < 1:1369242/68‑1 (MQ=255) tGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTtcatc > 1:1914037/1‑68 (MQ=255) tGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTtcatc > 1:1910183/1‑68 (MQ=255) gcgcCGCAGTTTTTCGGTATCTTTTTTCCAGCGTTCATTAGCCTGGTTGATATCGTCAATTTTCATCaa > 1:930925/1‑69 (MQ=255) gcgcCGCAGTTGTTCGGTATCTTTTTTCCTGCGTTCATTAGCCTGGTTGATATCGTCAATTTTCATCaa > 1:250745/1‑69 (MQ=255) gcgcCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTTCATCaa > 1:2832101/1‑69 (MQ=255) gcgcCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTTCATCaa > 1:368381/1‑69 (MQ=255) gcgcCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTTCATCaa > 1:116163/1‑69 (MQ=255) gcgcCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTTCATCaa > 1:1529158/1‑69 (MQ=255) gcgcCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTTCATCaa > 1:3398061/1‑69 (MQ=255) gcgcCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTTCATCaa > 1:2949848/1‑69 (MQ=255) gcgcCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTTCATCaa > 1:886951/1‑69 (MQ=255) gcgcCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTTCATCaa > 1:1685525/1‑69 (MQ=255) gcgcCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCCTGGTTGATATCGTCAATTTtcataa > 1:13994/1‑66 (MQ=255) gcgcCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCCTGGTTGATATCGTCAATTTTCATCaa > 1:599114/1‑69 (MQ=255) gcgcCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCCTGGTTGATATCGTCAATTTTCATCaa > 1:1127965/1‑69 (MQ=255) gcgcCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCCTGGTTGATATCGTCAATTTTCATCaa > 1:2258232/1‑69 (MQ=255) gcgcCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCCTGGTTGATATCGTCAATTTTCATCaa > 1:2245561/1‑69 (MQ=255) gcgcCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCCTGGTTGATATCGTCAATTTTCATCaa > 1:610098/1‑69 (MQ=255) gcgcCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCCTGGTTGATATCGTCAATTTTCATCaa > 1:82640/1‑69 (MQ=255) gcgcCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCCTGGTTGATATCGTCAATTTTCATCaa > 1:2741983/1‑69 (MQ=255) gcgcCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCCTGGTTGATATCGTCAATTTTCATCaa > 1:1809747/1‑69 (MQ=255) gcgcCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCCTGGTTGATATCGTCAATTTTCATCaa > 1:1363201/1‑69 (MQ=255) tgttCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTTCATCAAAATCGCGACt > 1:553014/1‑69 (MQ=255) tgttCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTTCATCAAAATCGCGACt > 1:2689075/1‑69 (MQ=255) tgttCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTTCATCAAAATCGCGACt > 1:142267/1‑69 (MQ=255) tgttCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTTCATCAAAATCGCGACt > 1:1962847/1‑69 (MQ=255) tgttCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTTCATCAAAATCGCGACt > 1:978102/1‑69 (MQ=255) gTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTTCATCAAAATCGCGACtttttt < 1:1642559/68‑1 (MQ=255) gTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTTCATCAAAATCGCGACtttttt < 1:3167997/68‑1 (MQ=255) gTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTTCATCAAAATCGCGACtttttt < 1:983642/68‑1 (MQ=255) gTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTTCATCAAAATCGCGACtttttt < 1:2904555/68‑1 (MQ=255) gTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTTCATCAAAATCGCGACtttttt < 1:1573848/68‑1 (MQ=255) gTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTTCATCAAAATCGCGACtttttt < 1:2491016/68‑1 (MQ=255) gTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTTCATCAAAATCGCGACtttttt < 1:27227/68‑1 (MQ=255) tATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTTCATCAAAATCGCGACtttttt < 1:2053203/67‑1 (MQ=255) aTCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTTCATCAAAATCGCGACtttttt < 1:2512241/66‑1 (MQ=255) ttttttCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTTCATCAAAATCGCGACTTTTTTACTGtt > 1:2870637/1‑69 (MQ=255) ttttttCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTTCATCAAAATCGCGACTTTTTTACTGtt > 1:1304936/1‑69 (MQ=255) ttttttCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTTCATCAAAATCGCGACTTTTTTACTGtt > 1:1455401/1‑69 (MQ=255) ttttttCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTTCATCAAAATCGCGACTTTTTTACTGtt > 1:378299/1‑69 (MQ=255) ttttttCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTTCATCAAAATCGCGACTTTTTTACTGtt > 1:1435955/1‑69 (MQ=255) ttttttCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTTCATCAAAATCGCGACTTTTTTACTGtt > 1:485248/1‑69 (MQ=255) ttttttCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTTCATCAAAATCGCGACTTTTTTACTGtt > 1:3186579/1‑69 (MQ=255) ttttttCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTTCATCAAAATCGCGACTTTTTTACTGtt > 1:2053749/1‑69 (MQ=255) aGCTTGGTTGATATCGTCAATTTTCATCAAAATCGCGACTTTTTTACTGtt < 1:2954764/51‑1 (MQ=255) aGCTTGGTTGATATCGTCAATTTTCATCAAAATCGCGACTTTTTTACTGtt < 1:1718569/51‑1 (MQ=255) aGCTTGGTTGATATCGTCAATTTTCATCAAAATCGCGACTTTTTTACTGtt < 1:1119207/51‑1 (MQ=255) aGCTTGGTTGATATCGTCAATTTTCATCAAAATCGCGACTTTTTTACTGtt < 1:737923/51‑1 (MQ=255) aGCTTGGTTGATATCGTCAATTTTCATCAAAATCGCGACTTTTTTACTGtt < 1:731207/51‑1 (MQ=255) aGCTTGGTTGATATCGTCAATTTTCATCAAAATCGCGACTTTTTTACTGtt < 1:2391190/51‑1 (MQ=255) aGCTTGGTTGATATCGTCAATTTTCATCAAAATCGCGACTTTTTTACTGtt < 1:2769293/51‑1 (MQ=255) aGCTTGGTTGATATCGTCAATTTTCATCAAAATCGCGACTTTTTTACTGTTTATTTCTGAAACTTTCAt > 1:673911/1‑69 (MQ=255) aGCTTGGTTGATATCGTCAATTTTCATCAAAATCGCGACTTTTTTACTGTTTATTTCTGAAACTTTCAt > 1:2407731/1‑69 (MQ=255) aGCTTGGTTGATATCGTCAATTTTCATCAAAATCGCGACTTTTTTACTGTTTATTTCTGAAACTTTCAt > 1:2511015/1‑69 (MQ=255) aGCTTGGTTGATATCGTCAATTTTCATCAAAATCGCGACTTTTTTACTGTTTATTTCTGAAACTTTCAt > 1:1020472/1‑69 (MQ=255) aGCTTGGTTGATATCGTCAATTTTCATCAAAATCGCGACTTTTTTACTGTTTATTTCTGAAACTTTCAt > 1:1223726/1‑69 (MQ=255) aGCTTGGTTGATATCGTCAATTTTCATCAAAATCGCGACTTTTTTACTGTTTATTTCTGAAACTTTCAt > 1:2708296/1‑69 (MQ=255) aGCTTGGTTGATATCGTCAATTTTCATCAAAATCGCGACTTTTTTACTGTTTATTTCTGAAACTTTCAt > 1:1723569/1‑69 (MQ=255) aGCTTGGTTGATATCGTCAATTTTCATCAAAATCGCGACTTTTTTACTGTTTATTTCTGAAACTTTCAt > 1:1102280/1‑69 (MQ=255) aGCTTGGTTGATATCGTCAATTTTCATCAAAATCGCGACTTTTTTACTGTTTATTTCTGAAACTTTCAt > 1:1280580/1‑69 (MQ=255) aGCTTGGTTGATATCGTCAATTTTCATCAAAATCGCGACTTTTTTACTGTTTATTTCTGAAACTTTCAt > 1:3355869/1‑69 (MQ=255) | TATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTTCATCAAAATCGCGACTTTTTTACTGTTTATTTCTGAAACTTTCAT > minE/1985544‑1985677 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |