| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,986,044 | T→A | 66.7% | E172D (GAA→GAT) | yggM ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,986,044 | 0 | T | A | 66.7% | 40.9 / 31.5 | 45 | E172D (GAA→GAT) | yggM | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (10/5); new base A (17/13); total (27/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.48e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.15e-01 | |||||||||||
TTTTCCCCAATATGCATCCGCTGCTTTCTGCGCCGCCACCATTTGCTTATCAAGCGCTTTCACTTCCTTTTCCAGCGCAGGAATCGAGTTCTTCTGGGTATTCAACGCAGATTCAAGCTGACTAAAGCGAT > minE/1985981‑1986111 | ttttCCCCAATATGCATCCGCTGCTTTCTGCGCCGCCACCATTTGCTTATCAAGCGCTTTCACTTCCt > 1:1837384/1‑68 (MQ=255) atatGCATCCGCTGCTTTCTGCGCCGCCACCATTTGCTTATCAAGCGCTTTTACATCCTTTTTCAgtgc < 1:2843065/69‑1 (MQ=255) atatGCATCCGCTGCTTTCTGCGCCGCCACCATTTGCTTATCAAGCGCTTTTACATCCTTTTTCAgtgc < 1:1830792/69‑1 (MQ=255) atatGCATCCGCTGCTTTCTGCGCCGCCACCATTTGCTTATCAAGCGCTTTTACATCCTTTTTCAgtgc < 1:1720083/69‑1 (MQ=255) atatGCATCCGCTGCTTTCTGCGCCGCCACCATTTGCTTATCAAGCGCTTTTACATCCTTTTTCAgtgc < 1:2173780/69‑1 (MQ=255) atatGCATCCGCTGCTTTCTGCGCCGCCACCATTTGCTTATCAAGCGCTTTTACATCCTTTTTCAgtgc < 1:2255670/69‑1 (MQ=255) atatGCATCCGCTGCTTTCTGCGCCGCCACCATTTGCTTATCAAGCGCTTTTACATCCTTTTTCAgtgc < 1:1460928/69‑1 (MQ=255) atatGCATCCGCTGCTTTCTGCGCCGCCACCATTTGCTTATCAAGCGCTTTTACATCCTTTTTCAgtgc < 1:2670254/69‑1 (MQ=255) atatGCATCCGCTGCTTTCTGCGCCGCCACCATTTGCTTATCAAGCGCTTTTACATCCTTTTTCAgtgc < 1:2887805/69‑1 (MQ=255) atatGCATCCGCTGCTTTCTGCGCCGCCACCATTTGCTTATCAAGCGCTTTTACATCCTTTTTCAgtgc < 1:1252575/69‑1 (MQ=255) atatGCATCCGCTGCTTTCTGCGCCGCCACCATTTGCTTATCAAGCGCTTTTACATCCTTTTTCAgtgc < 1:1191917/69‑1 (MQ=255) atatGCATCCGCTGCTTTCTGCGCCGCCACCATTTGCTTATCAAGCGCTTTTACATCCTTTTTCAgtgc < 1:942448/69‑1 (MQ=255) atatGCATCCGCTGCTTTCTGCGCCGCCACCATTTGCTTATCAAGCGCTTTTACATCCTTTTTCAgtgc < 1:1104174/69‑1 (MQ=255) tGCATCCGCTGCTTTCTGCGCCGCCACCATTTGCTTATCAAGCGCTTTCACTTCCTTTTCCAGCGCAgg > 1:2245285/1‑69 (MQ=255) tGCATCCGCTGCTTTCTGCGCCGCCACCATTTGCTTATCAAGCGCTTTCACTTCCTTTTCCAGCGCAgg > 1:3139981/1‑69 (MQ=255) tGCATCCGCTGCTTTCTGCGCCGCCACCATTTGCTTATCAAGCGCTTTCACTTCCTTTTCCAGCGCAgg > 1:2412579/1‑69 (MQ=255) tGCATCCGCTGCTTTCTGCGCCGCCACCATTTGCTTATCAAGCGCTTTCACTTCCTTTTCCAGCGCAgg > 1:599758/1‑69 (MQ=255) tGCATCCGCTGCTTTCTGCGCCGCCACCATTTGCTTATCAAGCGCTTTCACTTCCTTTTCCAGCGCAgg > 1:974991/1‑69 (MQ=255) tGCATCCGCTGCTTTCTGCGCCGCCACCATTTGCTTATCAAGCGCTTTCACTTCCTTTTCCAGCGCAgg > 1:1798388/1‑69 (MQ=255) ctgctTTCTGCGCCGCCACCATTTGCTTATCAAGCGCTTTCACTTCCTTTTCCAGCGCAGGAATCGAg < 1:2915719/68‑1 (MQ=255) gctTTCTGGGCCGCCACCATTTGCTTATCAAGCGCTTTTACATCCTTTTTCAGTGCAGGAATCGAGtta > 1:780909/1‑68 (MQ=255) gctTTCTGCGCCGCCACCATTTGCTTATCAAGCGCTTTTACATCCTTTTTCAGTGCAGGAATCGAGtta > 1:1744781/1‑68 (MQ=255) gctTTCTGCGCCGCCACCATTTGCTTATCAAGCGCTTTTACATCCTTTTTCAGTGCAGGAATCGAGtta > 1:1087351/1‑68 (MQ=255) gctTTCTGCGCCGCCACCATTTGCTTATCAAGCGCTTTTACATCCTTTTTCAGTGCAGGAATCGAGtta > 1:844404/1‑68 (MQ=255) gctTTCTGCGCCGCCACCATTTGCTTATCAAGCGCTTTTACATCCTTTTTCAGTGCAGGAATCGAGtta > 1:774779/1‑68 (MQ=255) gctTTCTGCGCCGCCACCATTTGCTTATCAAGCGCTTTTACATCCTTTTTCAGTGCAGGAATCGAGtta > 1:2372509/1‑68 (MQ=255) gctTTCTGCGCCGCCACCATTTGCTTATCAAGCGCTTTTACATCCTTTTTCAGTGCAGGAATCGAGtta > 1:2182394/1‑68 (MQ=255) gctTTCTGCGCCGCCACCATTTGCTTATCAAGCGCTTTTACATCCTTTTTCAGTGAAGGAATCGAGtt > 1:1512934/1‑68 (MQ=255) ttCTGCGCCGCCACCATTTGCTTATCAAGCGCTTTCACTTCCTTTTCCAGCGCAGGAATCGAGttct < 1:3365149/67‑1 (MQ=255) ttCTGCGCCGCCACCATTTGCTTATCAAGCGCTTTCACTTCCTTTTCCAGCGCAGGAATCGAGttct < 1:3300885/67‑1 (MQ=255) ttCTGCGCCGCCACCATTTGCTTATCAAGCGCTTTCACTTCCTTTTCCAGCGCAGGAATCGAGttct < 1:1425576/67‑1 (MQ=255) aTTTGCTTATCAAGCGCTTTCACTTCCTTTTCCAGCGCAGGAATCGAGTTCTTCTGGGTATTCaa > 1:1742798/1‑65 (MQ=255) tttGCTTATCAAGCGCTTTTACATCCTTTTTCAgtgc < 1:599951/37‑1 (MQ=25) gCTTATCAAGCGCTTTTACATCCTTTTTCAGTGCAGGAATCGAGTTATTCTGGTTATTCAACGCAGac > 1:1179797/1‑67 (MQ=255) gCTTATCAAGCGCTTTTACATCCTTTTTCAGTGCAGGAATCGAGTTATTCTGGTTATTCAACGCAGac > 1:1377346/1‑67 (MQ=255) gCTTATCAAGCGCTTTTACATCCTTTTTCAGTGCAGGAATCGAGTTATTCTGGTTATTCAACGCAGac > 1:1576701/1‑67 (MQ=255) gCTTATCAAGCGCTTTTACATCCTTTTTCAGTGCAGGAATCGAGTTATTCTGGTTATTCAACGCAGac > 1:3087762/1‑67 (MQ=255) gCTTATCAAGCGCTTTTACATCCTTTTTCAGTGCAGGAATCGAGTTATTCTGGTTATTCAACGCAGac > 1:2299802/1‑67 (MQ=255) gCTTATCAAGCGCTTTTACATCCTTTTTCAGTGCAGGAATCGAGTTATTCTGGTTATTCAACGCAGac > 1:2635548/1‑67 (MQ=255) gCTTATCAAGCGCTTTTACATCCTTTTTCAGTGCAGGAATCGAGTTATTCTGGTTATTCAACGCAGac > 1:2419848/1‑67 (MQ=255) gCTTATCAAGCGCTTTTACATCCTTTTTCAGTGCAGGAATCGAGTTATTCTGGTTATTCAACGCAGac > 1:167512/1‑67 (MQ=255) gCTTATCAAGCGCTTTTACATCCTTTTTCAGTGCAGGAATCGAGTTATTCTGGTTATTCAACGCAGac > 1:1064625/1‑67 (MQ=255) aTCAAGCGCTTTCACTTCCTTTTCCAGCGCAGGAATCGAGTTCTTCTGGGTATTCAACGCAGATTCAAg > 1:1313111/1‑69 (MQ=255) aTCAAGCGCTTTCACTTCCTTTTCCAGCGCAGGAATCGAGTTCTTCTGGGTATTCAACGCAGATTCAAg > 1:124685/1‑69 (MQ=255) gcTTTCACTTCCTTTTCCAGCGCAGGAATCGAGttct < 1:1458607/37‑1 (MQ=255) ttCCTTTTCCAGCGCAGGAATCGAGTTCTTCTGGGTATTCAACGCAGATTCAAGCTGACTAAAGCGAt < 1:2376246/68‑1 (MQ=255) | TTTTCCCCAATATGCATCCGCTGCTTTCTGCGCCGCCACCATTTGCTTATCAAGCGCTTTCACTTCCTTTTCCAGCGCAGGAATCGAGTTCTTCTGGGTATTCAACGCAGATTCAAGCTGACTAAAGCGAT > minE/1985981‑1986111 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |