| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 160,302 | T→C | 31.1% | G198G (GGT→GGC) | degP → | serine endoprotease (protease Do), membrane‑associated |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 160,302 | 0 | T | C | 31.1% | 65.6 / 36.8 | 77 | G198G (GGT→GGC) | degP | serine endoprotease (protease Do), membrane‑associated |
| Reads supporting (aligned to +/- strand): ref base T (32/21); new base C (12/12); total (44/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.60e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.06e-01 | |||||||||||
AAGATGGCGGATTCTGATGCACTGCGCGTGGGTGATTACACCGTAGCGATTGGTAACCCGTTTGGTCTGGGCGAGACGGTAACTTCCGGGATTGTCTCTGCGCTGGGGCGTAGCGGCCTGAATGCC > minE/160237‑160362 | aaGATGTCGGATTCTGATGCACTGCGCGTGGGTGATTACACCGTAGCGATTGGTAACCCGTTTGGTCTg > 1:699/1‑69 (MQ=255)aaGATGGCGGATTCTGATGCACTGCGCGTGGGTGATTACACCGTAGCGATTGGTAACCCGTTTGGTCt > 1:3059500/1‑68 (MQ=255)aaGATGGCGGATTCTGATGCACTGCGCGTGGGTGATTACACCGTAGCGATTGGTAACCCGTTTGGTCt > 1:2971326/1‑68 (MQ=255)aaGATGGCGGATTCTGATGCACTGCGCGTGGGTGATTACACCGTAGCGATTGGTAACCCGTTTGGTCTg > 1:3362245/1‑69 (MQ=255)aaGATGGCGGATTCTGATGCACTGCGCGTGGGTGATTACACCGTAGCGATTGGTAACCCGTTTGGTCTg > 1:3105240/1‑69 (MQ=255)aaGATGGCGGATTCTGATGCACTGCGCGTGGGTGATTACACCGTAGCGATTGGTAACCCGTTTGGTCTg > 1:2174646/1‑69 (MQ=255) ggCGGATTCTGATGTACTGCGCGTGGGTGATTACACCGTAGCGATTGGTAACCCGTTTGGTCTGGGCga > 1:3250915/1‑69 (MQ=255) ggCGGATTCTGATGCACTGCGCGTGGGTGATTACACCGTAGCGATTGGTAACCCGTTTGGTCTGGGc > 1:2551810/1‑67 (MQ=255) ggCGGATTCTGATGCACTGCGCGTGGGTGATTACACCGTAGCGATTGGTAACCCGTTTGGTCTGGGCga > 1:1485344/1‑69 (MQ=255) ggCGGATTCTGATGCACTGCGCGTGGGTGATTACACAGTAGCGATTGGTAACCCGTTTGGTCTGGGCga > 1:97754/1‑69 (MQ=255) ttCTGATGCACTGCGCGTGGGTGATTACACCGTAGCGATTGGTAACCCGTTTGGTCTGGGCGAGACGGt > 1:1119617/1‑69 (MQ=255) ttCTGATGCACTGCGCGTGGGTGATTACACCGTAGCGATTGGTAACCCGTTTGGTCTGGGCGAGACGGt > 1:2963544/1‑69 (MQ=255) ttCTGATGCACTGCGCGTGGGTGATTACACCGTAGCGATTGGTAACCCGTTTGGTCTGGGCGAGACGGt > 1:2763561/1‑69 (MQ=255) ttCTGATGCACTGCGCGTGGGTGATTACACCGTAGCGATTGGTAACCCGTTTGGTCTGGGCGAGACGGt > 1:2187001/1‑69 (MQ=255) ttCTGATGCACTGCGCGTGGGTGATTACACCGTAGCGATTGGTAACCCGTTTGGTCTGGGCGAGACGGt > 1:1843399/1‑69 (MQ=255) ttCTGATGCACTGCGCGTGGGTGATTACACCGTAGCGATTGGTAACCCGTTTGGTCTGGGCGAGACGGt > 1:1181201/1‑69 (MQ=255) tCTGCTGCACTGCGCGTGGGTGATTACACCGTAGCGATTGGTAACCCGTTTGGTCTGGGCGAGACGGTa > 1:2782648/1‑69 (MQ=255) tCTGATGCACTGCGCGTGGGTGATTACACCGTAGCGATTGGTAACCCGTTTGGTCTGGGCGAGACGGTa > 1:9450/1‑69 (MQ=255) tCTGATGCACTGCGCGTGGGTGATTACACCGTAGCGATTGGTAACCCGTTTGGTCTGGGCGAGACGGTa > 1:3011616/1‑69 (MQ=255) tCTGATGCACTGCGCGTGGGTGATTACACCGTAGCGATTGGTAACCCGTTTGGTCTGGGCGAGACGGTa > 1:1637256/1‑69 (MQ=255) ggTGATTACACCGTAGCAATTGGTAACCCGTTCGGCCTGGGCgaga < 1:516895/46‑1 (MQ=37) ggTGATTACACCGTAGCAATTGGTAACCCGTTCGGCCTGGGCgaga < 1:294070/46‑1 (MQ=37) ggTGATTACACCGTAGCAATTGGTAACCCGTTCGGCCTGGGCgaga < 1:2642991/46‑1 (MQ=37) ggTGATTACACCGTAGCAATTGGTAACCCGTTCGGCCTGGGCgaga < 1:3160327/46‑1 (MQ=37) ggTGATTACACCGTAGCAATTGGTAACCCGTTCGGCCTGGGCgaga < 1:3232421/46‑1 (MQ=37) ggTGATTACACCGTAGCAATTGGTAACCCGTTCGGCCTGGGCgaga < 1:2369009/46‑1 (MQ=37) ggTGATTACACCGTAGCAATTGGTAACCCGTTCGGCCTGGGCgaga < 1:1243867/46‑1 (MQ=37) ggTGATTACACCGTAGCAATTGGTAACCCGTTCGGCCTGGGCgaga < 1:699760/46‑1 (MQ=37) ggTGATTACACCGTAGCAATTGGTAACCCGTTCGGCCTGGGCgaga < 1:763701/46‑1 (MQ=37) ggTGATTACACCGTAGCAATTGGTAACCCGTTCGGCCTGGGCgaga < 1:833395/46‑1 (MQ=37) ggTGATTACACCGTAGCAATTGGTAACCCGTTCGGCCTGGGCgaga < 1:196282/46‑1 (MQ=37) ggTGATTACACCGTAGCAATTGGTAACCCGTTCGGCCTGGCCgaga < 1:86214/46‑1 (MQ=25) tACACCGTAGCGATTGGTAACCCGTTTGGTCTGGGCGAGACGGTAACTTCCGGGATTGTCTCTGCGCTg > 1:2183200/1‑69 (MQ=255) tACACCGTAGCGATTGGTAACCCGTTTGGTCTGGGCGAGACGGTAACTTCCGGGATTGTCTCTGCGCTg > 1:2363276/1‑69 (MQ=255) tACACCGTAGCGATTGGTAACCCGTTTGGTCTGGGCGAGACGGTAACTTCCGGGATTGTCTCTGCGCTg > 1:1703304/1‑69 (MQ=255) tACACCGTAGCGATTGGTAACCCGTTTGGTCTGGGCGAGACGGTAACTTCCGGGATTGTCTCTGCGCTg > 1:2915109/1‑69 (MQ=255) acacCGTAGCGATTGGTAACCCGTTTGGTCTGGGCGAGACGGTAACTTCCTGGATTGTCTCTGCGCTgg > 1:94091/1‑69 (MQ=255) acacCGTAGCGATTGGTAACCCGTTTGGTCTGGGCGAGACGGTAACTTCCGGGATTGTCTCTGCGCTgg > 1:3034272/1‑69 (MQ=255) acacCGTAGCGATTGGTAACCCGTTTGGTCTGGGCGAGACGGTAACTTCCGGGATTGTCTCTGCGCTgg > 1:1049760/1‑69 (MQ=255) acacCGTAGCGATTGGTAACCCGTTTGGTCTGGGCGAGACGGTAACTTCCGGGATTGTCTCTGCGCTgg > 1:1627781/1‑69 (MQ=255) gATTGGTAACCCGTTTGGTCTGGGCGAGACGGTAACTTCCGGGATTGTCTCTGCGCTGGGGCGTAGCg < 1:2876187/68‑1 (MQ=255) gATTGGTAACCCGTTTGGTCTGGGCGAGACGGTAACTTCCGGGATTGTCTCTGCGCTGGGGCGTAGCg < 1:2245263/68‑1 (MQ=255) gATTGGTAACCCGTTTGGTCTGGGCGAGACGGTAACTTCCGGGATTGTCTCTGCGCTGGGGCGTAGCg < 1:2186246/68‑1 (MQ=255) gATTGGTAACCCGTTTGGTCTGGGCGAGACGGTAACTTCCGGGATTGTCTCTGCGCTGGGGCGTAGCg < 1:2179985/68‑1 (MQ=255) gATTGGTAACCCGTTTGGTCTGGGCGAGACGGTAACTTCCGGGATTGTCTCTGCGCTGGGGCGTAGCg < 1:746971/68‑1 (MQ=255) gATTGGTAACCCGTTTGGTCTGGGCGAGACGGTAACTTCCGGGATTGTCTCTGCGCTGGGGCGTAGCg < 1:2136866/68‑1 (MQ=255) gATTGGTAACCCGTTTGGTCTGGGCGAGACGGTAACTTCCGGGATTGTCTCTGCGCTGGGGCGTAGCg < 1:1824118/68‑1 (MQ=255) ggTAACCCGTTTGGTCTGGGCGAGACGGTAACTTCCGTGATTGTCTCTGCGCTGGGGCGTAGCGGCCTg < 1:364121/69‑1 (MQ=255) ggTAACCCGTTTGGTCTGGGCGAGACGGTAACTTCCGGGATTGTCTCTGCGCTGGGGCGTAGCGGCCTg < 1:2098469/69‑1 (MQ=255) ggTAACCCGTTTGGTCTGGGCGAGACGGTAACTTCCGGGATTGTCTCTGCGCTGGGGCGTAGCGGCCTg < 1:842190/69‑1 (MQ=255) ggTAACCCGTTTGGTCTGGGCGAGACGGTAACTTCCGGGATTGTCTCTGCGCTGGGGCGTAGCGGCCTg < 1:2076570/69‑1 (MQ=255) ggTAACCCGTTTGGTCTGGGCGAGACGGTAACTTCCGGGATTGTCTCTGCGCTGGGGCGTAGCGGCCTg < 1:682127/69‑1 (MQ=255) ggTAACCCGTTTGGTCTGGGCGAGACGGTAACTTCCGGGATTGTCTCTGCGCTGGGGCGTAGCGGCCTg < 1:612967/69‑1 (MQ=255) ggTAACCCGTTTGGTCTGGGCGAGACGGTAACTTCCGGGATTGTCTCTGCGCTGGGGCGTAGCGGCCTg < 1:577497/69‑1 (MQ=255) ggTAACCCGTTTGGTCTGGGCGAGACGGTAACTTCCGGGATTGTCTCTGCGCTGGGGCGTAGCGGCCTg < 1:571055/69‑1 (MQ=255) ggTAACCCGTTTGGTCTGGGCGAGACGGTAACTTCCGGGATTGTCTCTGCGCTGGGGCGTAGCGGCCTg < 1:458936/69‑1 (MQ=255) ggTAACCCGTTTGGTCTGGGCGAGACGGTAACTTCCGGGATTGTCTCTGCGCTGGGGCGTAGCGGCCTg < 1:1560534/69‑1 (MQ=255) ggTAACCCGTTTGGTCTGGGCGAGACGGTAACTTCCGGGATTGTCTCTGCGCTGGGGCGTAGCGGCCTg < 1:265481/69‑1 (MQ=255) ggTAACCCGTTTGGTCTGGGCGAGACGGTAACTTCCGGGATTGTCTCTGCGCTGGGGCGTAGCGGCCTg < 1:2706649/69‑1 (MQ=255) ggTAACCCGTTTGGTCTGGGCGAGACGGTAACTTCCGGGATTGTCTCTGCGCTGGGGCGTAGCGGCCTg < 1:1375261/69‑1 (MQ=255) gTAACCCGTTTGGTCTGGGCGAGACGGTAACTTCCGGGATTGTCTCTGCGCTGGGGCGTAGCGGCCTg < 1:1361969/68‑1 (MQ=255) aCCCGTTCGGCCTGGGCGAGACAGTAACTTCCGGGATTGTCTCTGCGCTGGGACGt > 1:3241839/1‑56 (MQ=255) aCCCGTTCGGCCTGGGCGAGACAGTAACTTCCGGGATTGTCTCTGCGCTGGGACGTAGCGGCCTGaacg > 1:3132218/1‑67 (MQ=255) aCCCGTTCGGCCTGGGCGAGACAGTAACTTCCGGGATTGTCTCTGCGCTGGGACGTAGCGGCCTGaacg > 1:3128204/1‑67 (MQ=255) aCCCGTTCGGCCTGGGCGAGACAGTAACTTCCGGGATTGTCTCTGCGCTGGGACGTAGCGGCCTGaacg > 1:972563/1‑67 (MQ=255) aCCCGTTCGGCCTGGGCGAGACAGTAACTTCCGGGATTGTCTCTGCGCTGGGACGTAGCGGCCTGaacg > 1:925056/1‑67 (MQ=255) aCCCGTTCGGCCTGGGCGAGACAGTAACTTCCGGGATTGTCTCTGCGCTGGGACGTAGCGGCCTGaacg > 1:2956930/1‑67 (MQ=255) aCCCGTTCGGCCTGGGCGAGACAGTAACTTCCGGGATTGTCTATGCGCTGGGACGTAGCGGCCTGaacg > 1:261945/1‑67 (MQ=255) aCCCGTTCGGCCTGGGCGAGACAGTAACTTCCGGGATTGTCTATGCGCTGGGACGTAGCGGCCTGaacg > 1:1635896/1‑67 (MQ=255) aCCCGTTCGGCCTGGGCGAGACAGTAACTTCCGGGATTGTCTATGCGCTGGGACGTAGCGGCCTGaacg > 1:1422709/1‑67 (MQ=255) aCCCGTTCGGCCTGGGCGAGACAGTAACTTCCGGGATTGTCTATGCGCTGGGACGTAGCGGCCTGaacg > 1:14399/1‑67 (MQ=255) aCCCGTTCGGCCTGGGCGAGACAGTAACTTCCGGGATTGTCTATGCGCTGGGACGTAGCGGCCTGaacg > 1:2094666/1‑67 (MQ=255) aCCCGTTCGGCCTGGGCGAGACAGTAACTTCCGGGATTGTCTATGCGCTGGGACGTAGCGGCCTGaacg > 1:1642071/1‑67 (MQ=255) cGTTTGGTCTGGGCGAGACGGTAACTTCCGGGATTGTCTCTGCGCTGGGGCGTAGCGGCCTGAATGcc > 1:483689/1‑68 (MQ=255) cGTTTGGTCTGGGCGAGACGGTAACTTCCGGGATTGTCTCTGCGCTGGGGCGTAGCGGCCTGAATGcc > 1:967660/1‑68 (MQ=255) cGTTTGGTCTGGGCGAGACGGTAACTTCCGGGATTGTCTCTGCGCTGGGGCGTAGCGGCCTGAATGcc > 1:1426054/1‑68 (MQ=255) cGTTTGGTCTGGGCGAGACGGTAACTTCCGGGATTGTCTCTGCGCTGGGGCGTAGCGGCCTGAATGcc > 1:2640666/1‑68 (MQ=255) | AAGATGGCGGATTCTGATGCACTGCGCGTGGGTGATTACACCGTAGCGATTGGTAACCCGTTTGGTCTGGGCGAGACGGTAACTTCCGGGATTGTCTCTGCGCTGGGGCGTAGCGGCCTGAATGCC > minE/160237‑160362 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |