| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,987,528 | T→C | 26.0% | K65K (AAA→AAG) | ansB ← | periplasmic L‑asparaginase II |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,987,528 | 0 | T | C | 26.0% | 103.6 / 35.6 | 92 | K65K (AAA→AAG) | ansB | periplasmic L‑asparaginase II |
| Reads supporting (aligned to +/- strand): ref base T (48/20); new base C (22/2); total (70/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.04e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01 | |||||||||||
CAGACATTATCGTTCATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACTGTGTAGTTAGATTTG > minE/1987460‑1987594 | cAGACATTATCGTTCATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCt < 1:1245805/69‑1 (MQ=255) aTTATCGTTCATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAg > 1:2434283/1‑69 (MQ=255) aTTATCGTTCATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAg > 1:843496/1‑69 (MQ=255) aTTATCGTTCATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAg > 1:3204337/1‑69 (MQ=255) aTTATCGTTCATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAg > 1:1532010/1‑69 (MQ=255) ttATCGTTCATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGt < 1:2665741/69‑1 (MQ=255) ttATCGTTCATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGt < 1:2853530/69‑1 (MQ=255) ttATCGTTCATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGt < 1:1486874/69‑1 (MQ=255) aTCGTTCATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCtttt > 1:408731/1‑64 (MQ=255) aTCGTTCATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGtt > 1:2620362/1‑68 (MQ=255) aTCGTTCATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTg > 1:996662/1‑69 (MQ=255) aTCGTTCATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTg > 1:2215833/1‑69 (MQ=255) aTCGTTCATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTg > 1:2139675/1‑69 (MQ=255) aTCGTTCATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTg > 1:1886598/1‑69 (MQ=255) aTCGTTCATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTg > 1:3208390/1‑69 (MQ=255) aTCGTTCATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTg > 1:1123337/1‑69 (MQ=255) aTCGTTCATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTg > 1:507910/1‑69 (MQ=255) aTCGTTCATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGAAATGTCTTTTAGTTg > 1:1208726/1‑69 (MQ=255) cATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCAc < 1:3050328/69‑1 (MQ=255) cATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCAc < 1:2453616/69‑1 (MQ=255) ccGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGa < 1:3395006/69‑1 (MQ=255) atTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTc < 1:156288/69‑1 (MQ=255) atTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTc < 1:222840/69‑1 (MQ=255) atTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTc < 1:3049464/69‑1 (MQ=255) atTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTc < 1:3337327/69‑1 (MQ=255) ttCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTc < 1:1440053/68‑1 (MQ=255) cACTACCTGCTCGCCTTTAACGTTCGCAATGTCtttt < 1:244699/37‑1 (MQ=255) cACTACCTGCTCGCCTTTAACGTTCGCAATGTCtttt < 1:1988819/37‑1 (MQ=255) cACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTa > 1:2689205/1‑38 (MQ=255) cACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTAc > 1:2644251/1‑69 (MQ=255) cACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTAc > 1:680615/1‑69 (MQ=255) cACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTAc > 1:612432/1‑69 (MQ=255) cACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTAc > 1:2818299/1‑69 (MQ=255) cACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTAc > 1:366330/1‑69 (MQ=255) cACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTAc > 1:2872762/1‑69 (MQ=255) cACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTAc > 1:3206587/1‑69 (MQ=255) cACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTAc > 1:1105818/1‑69 (MQ=255) cACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTAc > 1:2399513/1‑69 (MQ=255) cACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTAc > 1:1448924/1‑69 (MQ=255) cACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTAc > 1:1274082/1‑69 (MQ=255) cACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTAc > 1:1872285/1‑69 (MQ=255) cACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTAc > 1:1719500/1‑69 (MQ=255) cACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTAc > 1:1944416/1‑69 (MQ=255) cACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTAc > 1:1269966/1‑69 (MQ=255) cACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTAc > 1:234892/1‑69 (MQ=255) cACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTAc > 1:1597039/1‑69 (MQ=255) cACTACCAGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTAc > 1:3133770/1‑69 (MQ=255) cTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTc < 1:1272214/64‑1 (MQ=255) tAACGTTCGCAATGTCTTTTAGTTGCGGCAGCGCATTAACCAGATTTTCTACGCCAACTTTACCCACtg > 1:345563/1‑69 (MQ=255) tAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACtg > 1:2683782/1‑69 (MQ=255) tAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACtg > 1:1740392/1‑69 (MQ=255) tAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACtg > 1:545449/1‑69 (MQ=255) tAACGTTCGCAATGTCCTTCAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCaa < 1:2067361/57‑1 (MQ=255) tAACGTTCGCAATGTCCTTCAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCaa < 1:2628781/57‑1 (MQ=255) aaCGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACtg > 1:687544/1‑68 (MQ=255) aaCGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACtg > 1:2806792/1‑68 (MQ=255) gTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACTGTGTa < 1:2226694/69‑1 (MQ=255) cGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACt > 1:38681/1‑52 (MQ=255) cGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACt > 1:2365500/1‑52 (MQ=255) cGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACt > 1:2276528/1‑52 (MQ=255) cGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACTGTGTa > 1:560734/1‑66 (MQ=255) cAATGTCCTTCAGTTGCGGCACCGCATTAACCAGAtttt > 1:41301/1‑39 (MQ=37) cAATGTCCTTCAGTTGCGGCACCGCATTAACCAGAtttt > 1:545743/1‑39 (MQ=37) cAATGTCCTTCAGTTGCGGCACCGCATTAACCAGAtttt > 1:709168/1‑39 (MQ=37) cAATGTCCTTCAGTTGCGGCACCGCATTAACCAGAtttt > 1:836192/1‑39 (MQ=37) cAATGTCCTTCAGTTGCGGCACCGCATTAACCAGAtttt > 1:1396192/1‑39 (MQ=37) cAATGTCCTTCAGTTGCGGCACCGCATTAACCAGAtttt > 1:867009/1‑39 (MQ=37) cAATGTCCTTCAGTTGCGGCACCGCATTAACCAGAtttt > 1:3379114/1‑39 (MQ=37) cAATGTCCTTCAGTTGCGGCACCGCATTAACCAGAtttt > 1:936675/1‑39 (MQ=37) cAATGTCCTTCAGTTGCGGCACCGCATTAACCAGAtttt > 1:1697325/1‑39 (MQ=37) cAATGTCCTTCAGTTGCGGCACCGCATTAACCAGAtttt > 1:3261777/1‑39 (MQ=37) cAATGTCCTTCAGTTGCGGCACCGCATTAACCAGAtttt > 1:2387882/1‑39 (MQ=37) cAATGTCCTTCAGTTGCGGCACCGCATTAACCAGAtttt > 1:3204049/1‑39 (MQ=37) cAATGTCCTTCAGTTGCGGCACCGCATTAACCAGAtttt > 1:2424349/1‑39 (MQ=37) cAATGTCCTTCAGTTGCGGCACCGCATTAACCAGAtttt > 1:2192905/1‑39 (MQ=37) cAATGTCCTTCAGTTGCGGCACCGCATTAACCAGAtttt > 1:3202565/1‑39 (MQ=37) cAATGTCCTTCAGTTGCGGCACCGCATTAACCAGAtttt > 1:2011172/1‑39 (MQ=37) cAATGTCCTTCAGTTGCGGCACCGCATTAACCAGAtttt > 1:1982762/1‑39 (MQ=37) cAATGTCCTTCAGTTGCGGCACCGCATTAACCAGAtttt > 1:2847171/1‑39 (MQ=37) cAATGTCCTTCAGTTGCGGCACCGCATTAACCAGAtttt > 1:2936026/1‑39 (MQ=37) cAATGTCCTTCAGTTGCGGCACCGCATTAACCAGAtttt > 1:17880/1‑39 (MQ=37) cAATGTCCTTCAGTTGCGGCACCGCATTAACCAGAtttt > 1:1827816/1‑39 (MQ=37) cAATGACCTTCAGTTGCGGCACCGCATTAACCAGAtttt > 1:1509455/1‑39 (MQ=25) gaTGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACTGTGTAGTTAg < 1:132769/67‑1 (MQ=255) aaTGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACTGTGTAGTTAg < 1:2702240/68‑1 (MQ=255) aaTGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACTGTGTAGTTAg < 1:3424011/68‑1 (MQ=255) aaTGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACTGTGTAGTTAg < 1:2231626/68‑1 (MQ=255) aaTGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACTGTGTAGTTAg < 1:88299/68‑1 (MQ=255) aaTGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACTGTGTAGTTAg < 1:10188/68‑1 (MQ=255) gTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACTGTGTAGTTAg < 1:1177671/65‑1 (MQ=255) tCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCGCTGTGTAGTTAGATTTg > 1:2566263/1‑69 (MQ=255) tCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACTGTGTAGTTAGATTTg > 1:317675/1‑69 (MQ=255) tCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACTGTGTAGTTAGATTTg > 1:3194385/1‑69 (MQ=255) tCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACTGTGTAGTTAGATTTg > 1:1436163/1‑69 (MQ=255) tCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACTGTGTAGTTAGATTTg > 1:3252358/1‑69 (MQ=255) tCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACTGTGTAGTTAGATTTg > 1:977032/1‑69 (MQ=255) | CAGACATTATCGTTCATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACTGTGTAGTTAGATTTG > minE/1987460‑1987594 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |